LOCUS       NM_025652               1315 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus general transcription factor III A (Gtf3a), mRNA.
ACCESSION   NM_025652
VERSION     NM_025652.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Wiese,C.B., Ireland,S., Fleming,N.L., Yu,J., Valerius,M.T.,
            Georgas,K., Chiu,H.S., Brennan,J., Armstrong,J., Little,M.H.,
            McMahon,A.P. and Southard-Smith,E.M.
  TITLE     A genome-wide screen to identify transcription factors expressed in
            pelvic Ganglia of the lower urinary tract
  JOURNAL   Front Neurosci 6, 130 (2012)
   PUBMED   22988430
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Guo,G., Huss,M., Tong,G.Q., Wang,C., Li Sun,L., Clarke,N.D. and
            Robson,P.
  TITLE     Resolution of cell fate decisions revealed by single-cell gene
            expression analysis from zygote to blastocyst
  JOURNAL   Dev Cell 18 (4), 675-685 (2010)
   PUBMED   20412781
REFERENCE   3  (bases 1 to 1315)
  AUTHORS   Hanas,J.S., Hocker,J.R., Cheng,Y.G., Lerner,M.R., Brackett,D.J.,
            Lightfoot,S.A., Hanas,R.J., Madhusudhan,K.T. and Moreland,R.J.
  TITLE     cDNA cloning, DNA binding, and evolution of mammalian transcription
            factor IIIA
  JOURNAL   Gene 282 (1-2), 43-52 (2002)
   PUBMED   11814676
REFERENCE   4  (bases 1 to 1315)
  AUTHORS   Moorefield,B. and Roeder,R.G.
  TITLE     Purification and characterization of human transcription factor
            IIIA
  JOURNAL   J Biol Chem 269 (33), 20857-20865 (1994)
   PUBMED   8063702
REFERENCE   5  (bases 1 to 1315)
  AUTHORS   Kozak,M.
  TITLE     Downstream secondary structure facilitates recognition of initiator
            codons by eukaryotic ribosomes
  JOURNAL   Proc Natl Acad Sci U S A 87 (21), 8301-8305 (1990)
   PUBMED   2236042
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BE656279.1, AF391799.1,
            AC124828.14 and BI202484.1.
            
            On Feb 8, 2008 this sequence version replaced NM_025652.2.
            
            Summary: The product of this gene is a zinc finger protein with
            nine Cis[2]-His[2] zinc finger domains. It functions as an RNA
            polymerase III transcription factor to induce transcription of the
            5S rRNA genes. The protein binds to a 50 bp internal promoter in
            the 5S genes called the internal control region (ICR), and
            nucleates formation of a stable preinitiation complex. This complex
            recruits the TFIIIC and TFIIIB transcription factors and RNA
            polymerase III to form the complete transcription complex. The
            protein is thought to be translated using a non-AUG translation
            initiation site in mammals based on sequence analysis, protein
            homology, and the size of the purified protein. [provided by
            RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF391799.1, AK008247.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: inferred from conservation
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BE656279.1         1-26
            27-514              AF391799.1         11-498
            515-515             AC124828.14        180354-180354
            516-1291            AF391799.1         500-1275
            1292-1315           BI202484.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 86.57 cM"
     gene            1..1315
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /note="general transcription factor III A"
                     /db_xref="GeneID:66596"
                     /db_xref="MGI:MGI:1913846"
     exon            1..319
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1219
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /note="non-AUG (CUG) translation initiation codon; TFIIIA;
                     factor A"
                     /codon_start=1
                     /product="transcription factor IIIA"
                     /protein_id="NP_079928.2"
                     /db_xref="CCDS:CCDS19873.2"
                     /db_xref="GeneID:66596"
                     /db_xref="MGI:MGI:1913846"
                     /translation="MEPRVSVAEAVSSLTIADAFVGACEGPAPPRPALPSRFICSFPD
                     CSASYNKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHVLIHTGEKPFVCAD
                     DGCNQKFNTKSNLKKHIERKHGNPQKQYVCSYEGCKKAFKKHQQLRTHQCQHTSEPLF
                     RCTHEGCGKHFASPSRLKRHGKVHEGYLCQKGCSFMGKTWTELLKHMREAHKEDITCN
                     VCQRMFKRRDYLKQHMKTHAPERDVYRCPRQGCGRTYTTVFNLQSHILSFHEEKRPFV
                     CEHAGCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRAPRERRSLASRLSGYFPPKRKQE
                     PDYSLPNASAESSSSPEAQLPPPAALLTVC"
     exon            320..420
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            421..517
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            518..606
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            607..680
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            681..761
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            762..991
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1051
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1298
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1271..1276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1291
                     /gene="Gtf3a"
                     /gene_synonym="2010015D03Rik; 2610111I01Rik;
                     5330403M05Rik"
                     /note="major polyA site"
ORIGIN      
        1 cctggagcct tagcgggccg cgttcccgga agttcaaagt ctccgggcgc cagtggcttg
       61 cgtcccgcgt tcccggggac ggcacgtggc tgccccgcgt cagcccgggc ccgatcgtgt
      121 cgccctggag ccgcgggtgt cagtcgcgga agcggtgtcg tcgctgacca tcgcggatgc
      181 gttcgtcggg gcctgtgagg gccccgcgcc gccgcgcccc gcgctgccca gcaggttcat
      241 ctgctccttt cccgactgca gcgccagtta caacaaagcc tggaagctag acgcgcacct
      301 atgcaaacac acgggggaga ggccatttgt ttgcgactat gagggctgtg gcaaggcctt
      361 catcagagac taccatctga gccggcatgt cctgattcac accggggaaa agccgtttgt
      421 ttgtgcagat gatggctgta atcagaaatt caacacaaaa tcaaacttga agaaacacat
      481 tgaacgcaaa catggaaacc cacaaaaaca gtatgtgtgc agttatgagg gttgcaagaa
      541 ggcctttaag aagcaccagc agctgagaac ccatcagtgc cagcacacca gcgagccgct
      601 cttcaggtgt acccacgagg gatgcgggaa gcactttgcc tcgcccagca ggctgaaacg
      661 gcatgggaaa gttcacgagg gctacctgtg tcaaaaggga tgttctttca tgggaaaaac
      721 gtggacagag ctcctgaaac acatgagaga agcccataaa gaggacataa cctgcaatgt
      781 atgtcagagg atgttcaagc gcagagatta ccttaagcag cacatgaaga ctcacgcccc
      841 ggaaagggat gtgtaccgct gtccgcggca aggctgcgga agaacctaca caaccgtgtt
      901 caacctgcag agccacattc tctccttcca cgaggaaaag cgcccatttg tgtgtgagca
      961 cgctggctgt ggcaagacat tcgcaatgaa acagagtctc atgaggcaca gtgtcgtgca
     1021 cgatcccgac aagaagagga tgaagctcaa agtaagagcc cctcgggaga gacgcagctt
     1081 ggcctctcgc ctcagtgggt acttccctcc taagaggaaa caagagcccg actactcctt
     1141 gcctaacgcc agcgcagagt ccagcagcag cccagaggcc cagctgcccc cgccagccgc
     1201 cttactcact gtctgctagg cgggaagact tcagggcggc cagcagaggc tcctggcctg
     1261 cacttttcct attaaagtca ctgacacaca acatccgaaa aaaaaaaaaa aaaaa
//
