LOCUS       NM_031391               5704 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus general transcription factor II A, 1 (Gtf2a1),
            transcript variant 1, mRNA.
ACCESSION   NM_031391
VERSION     NM_031391.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   El-Saafin,F., Bergamasco,M.I., Chen,Y., May,R.E., Esakky,P.,
            Hediyeh-Zadeh,S., Dixon,M., Wilcox,S., Davis,M.J., Strasser,A.,
            Smyth,G.K., Thomas,T. and Voss,A.K.
  TITLE     Loss of TAF8 causes TFIID dysfunction and p53-mediated apoptotic
            neuronal cell death
  JOURNAL   Cell Death Differ 29 (5), 1013-1027 (2022)
   PUBMED   35361962
REFERENCE   3  (bases 1 to 5704)
  AUTHORS   Niizuma,H., Searleman,A.C., Takeda,S., Armstrong,S.A., Park,C.Y.,
            Cheng,E.H. and Hsieh,J.J.
  TITLE     Taspase1 orchestrates fetal liver hematopoietic stem cell and
            vertebrae fates by cleaving TFIIA
  JOURNAL   JCI Insight 6 (15), e149382 (2021)
   PUBMED   34156981
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5704)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5704)
  AUTHORS   Takeda,S., Sasagawa,S., Oyama,T., Searleman,A.C., Westergard,T.D.,
            Cheng,E.H. and Hsieh,J.J.
  TITLE     Taspase1-dependent TFIIA cleavage coordinates head morphogenesis by
            limiting Cdkn2a locus transcription
  JOURNAL   J Clin Invest 125 (3), 1203-1214 (2015)
   PUBMED   25664857
  REMARK    GeneRIF: TFIIA is the principal TASP1 substrate that orchestrates
            craniofacial morphogenesis.
REFERENCE   6  (bases 1 to 5704)
  AUTHORS   Nakadai,T., Shimada,M., Shima,D., Handa,H. and Tamura,T.A.
  TITLE     Specific interaction with transcription factor IIA and localization
            of the mammalian TATA-binding protein-like protein (TLP/TRF2/TLF)
  JOURNAL   J Biol Chem 279 (9), 7447-7455 (2004)
   PUBMED   14570910
REFERENCE   7  (bases 1 to 5704)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 5704)
  AUTHORS   Han,S.Y., Zhou,L., Upadhyaya,A., Lee,S.H., Parker,K.L. and
            DeJong,J.
  TITLE     TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene
            whose expression is up-regulated with other general transcription
            factors during spermatogenesis in the mouse
  JOURNAL   Biol Reprod 64 (2), 507-517 (2001)
   PUBMED   11159353
REFERENCE   9  (bases 1 to 5704)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 5704)
  AUTHORS   Roeder,R.G.
  TITLE     The role of general initiation factors in transcription by RNA
            polymerase II
  JOURNAL   Trends Biochem Sci 21 (9), 327-335 (1996)
   PUBMED   8870495
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            EL956882.1, AK048711.1, BC079909.1, AW552430.1 and BX526091.1.
            
            On Apr 18, 2009 this sequence version replaced NM_031391.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.106173.1,
                                           SRR1660819.166022.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-370               EL956882.1         26-395              c
            371-1645            AK048711.1         359-1633
            1646-2926           BC079909.1         1579-2859
            2927-4230           BC079909.1         2861-4164
            4231-5059           BC079909.1         4166-4994
            5060-5649           AW552430.1         55-644              c
            5650-5704           BX526091.1         361-415
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 45.02 cM"
     gene            1..5704
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /note="general transcription factor II A, 1"
                     /db_xref="GeneID:83602"
                     /db_xref="MGI:MGI:1933277"
     exon            1..115
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1222
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /note="isoform 1 is encoded by transcript variant 1;
                     general transcription factor IIa, 1 (37kD and 19kD
                     subunits); transcription initiation factor IIA subunit 1;
                     TFIIA alpha/beta; general transcription factor IIA subunit
                     1"
                     /codon_start=1
                     /product="transcription initiation factor IIA subunit 1
                     isoform 1"
                     /protein_id="NP_113568.2"
                     /db_xref="CCDS:CCDS26089.1"
                     /db_xref="GeneID:83602"
                     /db_xref="MGI:MGI:1933277"
                     /translation="MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKT
                     LWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHQHQQAQPQQTVPQQAQ
                     TQQVLIPASQQATAPQVIVPDSKLLQHMNASSITSAAATAATLALPAGVTPVQQLLTN
                     SGQLLQVVRAANGAQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQ
                     TGVIIQPQQILFTGNKTQVIPTTVAAPAPAQAPMPAAGQQQPQAQPAQQQAPLVLQVD
                     GTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTE
                     NVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW"
     exon            116..217
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            218..425
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            426..493
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            494..569
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            570..703
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            704..1024
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1114
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..5704
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5677..5682
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /note="hexamer: AATAAA"
     polyA_site      5704
                     /gene="Gtf2a1"
                     /gene_synonym="6330549H03Rik; Tfiia1; TfIIAa/b"
                     /note="major polyA site"
ORIGIN      
        1 tttttttttt cttttttttt ttttttttaa attttttttt cctttctttc tatctcgttc
       61 cctcttgttt gtgttgtgtg tggaaatggc gaactcggca aacacaaaca ccgtgcctaa
      121 attgtataga tctgtgattg aagatgttat taatgacgta cgagacattt ttctggatga
      181 cggagtggat gaacaagtcc tgatggaact aaaaactttg tgggagaata aactaatgca
      241 gtctagggca gtagatggat ttcattcaga agaacagcag cttttgttgc aagttcagca
      301 gcagcatcaa ccccagcagc agcagcatca ccaccaccac caccagcacc agcaagcgca
      361 acctcagcaa acggtacctc agcaagcaca gacccagcag gtcctcattc ctgcatccca
      421 gcaagctaca gcaccacaag ttattgttcc tgattctaag ttgctacaac atatgaatgc
      481 atcaagtatt acgagtgctg ctgctacagc tgctaccttg gcactgcctg caggagtgac
      541 tccggttcaa cagctactaa caaattcagg ccagctgctt caggtggtca gagcagccaa
      601 tggtgcccag tatatcttgc agcctcagca gtcagttgtt ctacaacaac aagttatacc
      661 acaaatgcag cctggtggag tccaagctcc tgttatccag caggtcttag ccccacttcc
      721 tggagggatt tcaccacaga caggcgtcat catccagcca cagcaaatct tatttacagg
      781 aaataagact caggtcattc ctacaacagt ggcagcaccc gccccggcac aagcgccgat
      841 gcctgcagcc ggacagcagc agccacaggc ccagcctgcc caacagcaag ctccgttggt
      901 gctgcaagtt gatggaacag gggatacatc ttctgaggaa gatgaagatg aagaagaaga
      961 ctatgatgat gatgaggaag aagataaaga gaaagatgga gctgaagatg ggcaggtgga
     1021 agaagagccg ctcaacagcg aggatgacgt cagtgacgag gaagggcagg aactctttga
     1081 cactgagaat gttgtcgtct gccagtatga taagatacac agaagtaaaa ataaatggaa
     1141 atttcatctc aaggatggca ttatgaatct taacggaaga gattatatat tttccaaagc
     1201 cattggagat gcagagtggt gaagagttgc tcctttcctc ttataaatga gacaaagact
     1261 taaaaagtta aaatggacgg tttgaaactt gggacatcta tcagccaaaa cttcacttct
     1321 gcatgtcaga aaggcgcagt agaagcaaag ctcctggagc agagctaggc cttgaccaca
     1381 tagacactac ttcagaccgg caagccgtgg aactgtagca cctggggttg ctgggcgggg
     1441 ggaggggttg gtgtaggaaa actgtaactg tcgattttaa atacaggaac ctgccattgg
     1501 taattagcat gtaaagcttt gtctatattt gtttctccaa gttgggctca atcagaagat
     1561 atttgttgga atgacccgaa gaattaccct tttgatagac tgaactgaaa ttgcttattg
     1621 tatgtggtta tatttggtaa aaattgcgtg tgagcttttt tcagaagggc aagaattatg
     1681 gtgttgaatt ttaattcact tgtataagaa aatttaaaac tcataatagg tcttaaacac
     1741 atttttactt gtcactctcc tcagtactca tacctctcgc ttccctgctg tctgaacatc
     1801 tacgtaagat ttaaagaaaa ggatcgatgg gcacagcttg gagacgaagt tgacccagga
     1861 atggatgctt ggtttaatta ggttttcctg cttgttaaat gcaacttaaa gacttgggtc
     1921 ttacctaggt tgactagaat taaagtgaca gcttaatttc tgtacagaaa aactgctcag
     1981 tcccactcta taagaggaaa ccccaaatgg gctctcagga atctgttttc atctcacaaa
     2041 ggtcccatgg agctgcagtt gtacagcaca cgtgagatgt ggaggttttc cttctgggct
     2101 gcgtgtgtgc ttactgtgcc cgagtctgtg tgagccctgg gatgacagtc tgtgcgttgg
     2161 tctaagcata agggcaacgg atgaggactg gatttttatt aaacaattta gtaattataa
     2221 agtttcttgt gtttactagt aaaggatttt taaaattatt ttaattaaac aaaattttta
     2281 aaaaaaactt tgtaatttga gggaaaagtt tgagggtgga gggggtagag gaaagtgggc
     2341 cactaaatac aaatttacct ctttttcatt gggtggccct tccaggccat tggtgatgta
     2401 gacttgggct ccaatttgca cattggaaag aaaatacaga gatttgactt gtatatcaga
     2461 aattctagac atttactgtg tgttgagaat gtgtagctct gttcctgtaa ggggctccac
     2521 agaacaaaga ggcagcctcc tgggacctgc agggactctc gctcacacag ctcacttctg
     2581 ttctagtgac taacaacatt tgcattcgtt acattcattc gataacttgc ctgttgactt
     2641 tttttttttt ttgtcttcca tttttgtctt cctttatttt gactttatat agttctggaa
     2701 gtaacagata aagtatatca caaaagaaaa actgtgagct atattgtgct tttttaagaa
     2761 catattttaa attttgttaa tgcagttgat ttctcctccc cacttcgcac ccttctgaac
     2821 caatttatgt tgtgaaattt ggcatcagtg gtggggtcct cacatgatag gagttagagt
     2881 ttgcttgtat tccattactg gtaatacagg tttttttttt ttttttaatt ttgtttgttt
     2941 tcttaatgta gctttttgag gggaagttag tttaatctct tattctcaaa gcacacagca
     3001 gttctggttt aagtatagaa cttggcgaac aacagaatcc caggagttgc tgagttgtcc
     3061 ctccctcagt atagtctcta gtctgtgttt actcagtgag atcagtaacc tgaactacct
     3121 atggcagccc agcagtgctg ctccagtgaa acttgggcta atcagtagtt cttatgctaa
     3181 ttcagcacag gccctgcctc cctgtgtagt cagacatact gaaggttagc attgcccact
     3241 ggcctgtttc ttctagctgt acattgtcaa acatgaacaa ccacttcttt cagagtgaca
     3301 caaagggggc ttgtcttgaa gcaggaactg accatttata tttgtaccta tgtattgcct
     3361 aataatgaca gcgtcctgga ctaatccttt gatctgggaa agctctgaaa ggtggctcca
     3421 gcagccccgc actgtgctct tacgtgctgt tctgttgtgt tccatttaaa tcaagtagca
     3481 tgcggtgttc tatcgtagta tcgtagcttt tgctgtgtaa atgtcacttg ctttgcattc
     3541 tcaatcatgc tttcgccata acatttaact gatgatgtca tgaagttcta ataaagtgtc
     3601 attcctttgg aaagcaaggg attcccactt ggagagtttt aggatgaaag tagcaagttt
     3661 atccaaaagg tggaaccagg gacgcaagca gtgtcttcag aaactgatcc gtttcgacgt
     3721 gtacccagag tgacggcacc ttttatagga cacgatgcca gtagtgtgcc aaggtcagtt
     3781 cacatagatg actaacatgg aagtgtgtgc ctttcagtag atgagctctt gcctggtggt
     3841 catttgtatt ctcacaccac cggaatgccc gcgaacacac gcactttctt agtgacacaa
     3901 ctttgtcccg tgcagtttct agagtcaaac aggataggag gggatattta tatgaggcgt
     3961 ttgtatttga aatgttgaaa tttctgttaa ataagtctca catttacact gttgcagttt
     4021 taaaagtcag gctggcatca aaattcacga agcgtgtacc gagattgaaa tgtgttattg
     4081 acaagcatat tctggcttta gaagacaatg taaaaaagta ctagttttta tataaaggct
     4141 ttcttttgct ctattgaaaa tcaagttttt cttttggtgg cttgtatgga cgaatgcaga
     4201 gttgtctccc tctgagagtt tttttttttt atattatagc aataatgtca ccatattaga
     4261 ggataagtat accgtgtata agaacagacc tatctcctct tagcagtttt ccctttataa
     4321 cagcattttc ttccacttga gaaggctctt gtattttcat attacatcat atgagaatat
     4381 gaagcttttg agcttttgtg caatgtgtta ttttgttgat ctgtgtacat tttcatttca
     4441 tgtctccttc ctacctgtct gaatcagctg tatcagatgt ggaggttttg gttaatggtt
     4501 tgtgtgtttt ctcatcctta ttgttcagtt tagcttttca tctgttaccc ttatggaaat
     4561 tattctggtt tatattcttc ctcctggaag cccacctttg cccttctgtt atcttccttt
     4621 ggtgcatgat ttaaggaaga ctgcatcaaa tttaatattt ttagttttga agaataaaag
     4681 tttcctcccg tttgcgggga gaacaaacca ccattgaata aaccattggt ctttcagtca
     4741 agcttcttta gatcagttca gtgtgcatcg gttacaccgg gtgggagggg ccggccatag
     4801 ctgttcggag ctactattgt cgactgctgt tgtacagact gttctgtgta agggcgtgaa
     4861 tatatttatt atgtttgtaa aattcactct gtacacttgc agaccaaggt tgctcttctg
     4921 tgatatatcg ggtgttaagt tttaaagacc atcttggaat acaattaaag agcttagtat
     4981 tttgatgtac taagactatt ttaagtttta atattttacc ttgcaaaaac tttaattaaa
     5041 gatgttattt aaaaaaaaag taatgttgct tgctttgctt actagtcagc gacattggta
     5101 tatggacaac tgagtaaaac catacagttt agacaggaag ttcaacaaaa ataggatatc
     5161 gttaaatttt gtacccttag ttaaattctg tacctataag gcagtgggtt tggggtgcca
     5221 gactgaattg ttaaaactgt gttagaaatc cttaagcgct ttcaaaagtt gtattccagg
     5281 cacagaagca tttaggatgg gtaaattcac accaactaaa tgtaaattaa ggttaatata
     5341 attaacattg tattaaatga gtatttctac tcaaaaagct tagattacat aggatgaagt
     5401 tattaagaac attgtttcat ttgtttggct aaactgtttt ttctcagttc agagtttgta
     5461 aataattgct tattaggaga tttggattat tgaggtttgt attacatatt gaatatattt
     5521 tgtgttactt tagaagatat taaaattact agcagtattt tgattttata gttaattcaa
     5581 atgaatcatt aagagcttgc ctttttgtaa ttttttatcc tgttataaac tactttatct
     5641 aaggaaacat gcagatttta actattaaat aatcataata aagatatttt atttattgcc
     5701 agca
//
