LOCUS       NM_008180               1978 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus glutathione synthetase (Gss), transcript variant 1,
            mRNA.
ACCESSION   NM_008180
VERSION     NM_008180.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhu,H., Cheng,Y., Wang,X., Yang,X., Liu,M., Liu,J., Liu,S.,
            Wang,H., Zhang,A., Li,R., Ye,C., Zhang,J., Gao,J., Fu,X. and Wu,B.
  TITLE     Gss deficiency causes age-related fertility impairment via
            ROS-triggered ferroptosis in the testes of mice
  JOURNAL   Cell Death Dis 14 (12), 845 (2023)
   PUBMED   38114454
  REMARK    GeneRIF: Gss deficiency causes age-related fertility impairment via
            ROS-triggered ferroptosis in the testes of mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1978)
  AUTHORS   Orlowska,K., Fling,R.R., Nault,R., Schilmiller,A.L. and
            Zacharewski,T.R.
  TITLE     Cystine/Glutamate Xc- Antiporter Induction Compensates for
            Transsulfuration Pathway Repression by
            2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for
            Hepatic Glutathione Biosynthesis
  JOURNAL   Chem Res Toxicol 36 (6), 900-915 (2023)
   PUBMED   37184393
  REMARK    GeneRIF: Cystine/Glutamate Xc[-] Antiporter Induction Compensates
            for Transsulfuration Pathway Repression by
            2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for
            Hepatic Glutathione Biosynthesis.
REFERENCE   4  (bases 1 to 1978)
  AUTHORS   Celaya,A.M., Sanchez-Perez,I., Bermudez-Munoz,J.M., Rodriguez-de la
            Rosa,L., Pintado-Berninches,L., Perona,R., Murillo-Cuesta,S. and
            Varela-Nieto,I.
  TITLE     Deficit of mitogen-activated protein kinase phosphatase 1 (DUSP1)
            accelerates progressive hearing loss
  JOURNAL   Elife 8, e39159 (2019)
   PUBMED   30938680
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1978)
  AUTHORS   Wu,Y., Wei,F.Y., Kawarada,L., Suzuki,T., Araki,K., Komohara,Y.,
            Fujimura,A., Kaitsuka,T., Takeya,M., Oike,Y., Suzuki,T. and
            Tomizawa,K.
  TITLE     Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is
            Required for Mitochondrial Translation and Is Involved in
            Reversible Infantile Liver Injury
  JOURNAL   PLoS Genet 12 (9), e1006355 (2016)
   PUBMED   27689697
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1978)
  AUTHORS   Meixner,A., Karreth,F., Kenner,L., Penninger,J.M. and Wagner,E.F.
  TITLE     Jun and JunD-dependent functions in cell proliferation and stress
            response
  JOURNAL   Cell Death Differ 17 (9), 1409-1419 (2010)
   PUBMED   20300111
REFERENCE   7  (bases 1 to 1978)
  AUTHORS   Jackson,R.M. and Gupta,C.
  TITLE     Hypoxia and kinase activity regulate lung epithelial cell
            glutathione
  JOURNAL   Exp Lung Res 36 (1), 45-56 (2010)
   PUBMED   20128681
  REMARK    GeneRIF: Hypoxia decreased 2 key enzyme activities that regulate
            GSH synthesis, glutamate cysteine ligase (GCL) (E.C. 6.3.2.2) and
            glutathione synthase (GS) (E.C. 6.3.2.3)
REFERENCE   8  (bases 1 to 1978)
  AUTHORS   Rzucidlo,S.J. and Brackett,B.G.
  TITLE     Developmental patterns of zygotes from transgenic female mice with
            elevated tissue glutathione
  JOURNAL   J Exp Zool 286 (2), 173-180 (2000)
   PUBMED   10617859
REFERENCE   9  (bases 1 to 1978)
  AUTHORS   Sun,Y.
  TITLE     Induction of glutathione synthetase by 1,10-phenanthroline
  JOURNAL   FEBS Lett 408 (1), 16-20 (1997)
   PUBMED   9180259
REFERENCE   10 (bases 1 to 1978)
  AUTHORS   Shi,Z.Z., Carter,B.Z., Habib,G.M., He,X., Sazer,S., Lebovitz,R.M.
            and Lieberman,M.W.
  TITLE     A single mouse glutathione synthetase gene encodes six mRNAs with
            different 5' ends
  JOURNAL   Arch Biochem Biophys 331 (2), 215-224 (1996)
   PUBMED   8660701
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844852.11, AK169096.1 and AI846976.1.
            
            On Mar 26, 2014 this sequence version replaced NM_008180.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003784.1, AK169096.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AL844852.11        157283-157362       c
            81-1955             AK169096.1         1-1875
            1956-1978           AI846976.1         1-23                c
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 77.26 cM"
     gene            1..1978
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /note="glutathione synthetase"
                     /db_xref="GeneID:14854"
                     /db_xref="MGI:MGI:95852"
     exon            1..117
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            118..252
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..1548
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /EC_number="6.3.2.3"
                     /note="isoform a is encoded by transcript variant 1; GSH
                     synthetase; glutathione synthase"
                     /codon_start=1
                     /product="glutathione synthetase isoform a"
                     /protein_id="NP_032206.1"
                     /db_xref="CCDS:CCDS16951.1"
                     /db_xref="GeneID:14854"
                     /db_xref="MGI:MGI:95852"
                     /translation="MATSWGSILQDEKQLEELAKQAIDRALAEGVLLRSAQHPSSSDV
                     VTYAPFTLFPSPVPSALLEQAYAVQMDFNILVDAVSQNPAFLEQTLSSTIKKDDYTAR
                     LFDIYKQVLKEGIAQTVFLGLNRSDYMFQCGADGSKALKQIEINTISASFGGLASRTP
                     AVHRHVLNVLNKTKEASKILSNNPSKGLALGIAKAWELYGSANAVVLLIAQEKERNIF
                     DQRAVENELLDRKIHVIRGRFEDVSERGSLDQNRRLFMDDQEVAVVYFRDGYMPSQYN
                     SQNWEARLMLERSRAAKCPDIAIQLAGTKKVQQELSRVGLLEALLPGQPEAVARLRAT
                     FAGLYSLDMGEEGDQAIAEALAAPSHFVLKPQREGGGNNLYGEEMVQALEQLKDSEER
                     ASYILMEKIEPEPFRNCLLRPGSPAQVVQCISELGIFGVYVRQGTTLVMNKHVGHLLR
                     TKAVEHADGGVAAGVAVLDNPYPV"
     exon            253..398
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            399..474
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            475..614
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            615..731
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            732..812
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            813..890
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            891..957
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1152
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1234
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1424
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1963
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1940..1945
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /note="hexamer: AATAAA"
     polyA_site      1958
                     /gene="Gss"
                     /gene_synonym="GS-A/GS-B; GSH-S"
                     /note="major polyA site"
ORIGIN      
        1 ctttagcctt gcggggcggt ctgcgcaggc gcactgggtc gtaccgaagc cccgcccccc
       61 gagcccgggt aacggcgtgc ggagtttgag cttggcgagc agctggacaa cgagcgagtt
      121 gggatggcta ccagctgggg cagcatcttg caggatgaga agcagctcga agaactggca
      181 aagcaggcca tagacagggc cctggccgag ggcgtgttgc tgaggtccgc acagcatccc
      241 agctcctccg acgtggtgac gtatgcccca ttcacgcttt tcccctcacc agtacccagt
      301 gctctgctgg agcaggccta tgctgtgcag atggacttca acatactggt ggatgctgtc
      361 agccagaacc cagccttcct ggagcaaaca ctgtctagca ccatcaaaaa ggacgactat
      421 actgcccgtc tctttgatat ctacaagcaa gtcctgaaag agggcattgc ccagaccgtg
      481 ttcctgggcc tgaatcgctc agattacatg ttccagtgcg gcgcagacgg ctccaaagcc
      541 ctgaaacaga tcgagatcaa cactatctct gccagctttg ggggcctggc ctcccggact
      601 ccagctgtgc accgacacgt tctcaatgtc ctgaataaga ccaaagaagc ttccaagatc
      661 ctgtccaata accccagcaa gggactggcc ctggggatcg ccaaagcctg ggagctctat
      721 ggctcagcta atgcggtggt gctactgatt gctcaagaga aggaaaggaa catatttgac
      781 cagcgtgccg tagagaacga gctgctagac aggaagatcc atgtcatccg tggaagattt
      841 gaagatgtct ctgaaagggg ttctctggac caaaaccgaa ggctgtttat ggatgaccag
      901 gaagttgctg tggtgtactt ccgagatggc tacatgccca gtcagtataa ttcacagaac
      961 tgggaagcac gcctgatgct agagagatct cgtgctgcca agtgtccaga cattgccata
     1021 cagctggctg ggactaagaa ggtgcagcag gaactgagca gggtgggtct gctggaagcg
     1081 ctgctcccgg gccagcctga ggctgtggcc cgcctccgag ccacctttgc tggcctctat
     1141 tcactggaca tgggtgaaga aggggaccag gccattgctg aggcccttgc tgctcctagc
     1201 cactttgtgc tgaagcccca gagagagggt ggaggtaaca acttatacgg ggaggaaatg
     1261 gtacaagctc tggagcagct gaaggacagt gaggagagag cctcctacat cctcatggag
     1321 aagattgaac ctgagccttt taggaattgc ttgctacggc ctggcagccc tgcccaagtg
     1381 gtccagtgta tctcggagct gggtattttt ggagtctatg tcagacaggg aacaacactg
     1441 gtgatgaaca agcatgtggg gcacctgctt cgaaccaaag ccgtggagca tgcagacgga
     1501 ggtgtggcgg caggagtggc agtcctggac aacccctacc ctgtgtgaag acgccatctg
     1561 gacttcactc aggaggcctt ctatcccctg tacttggcac tcctcttctg aggggttgcc
     1621 cctgtcccta tcttagggga gcttgtctct tccatagacc tccaaagctt cagggaaggg
     1681 aaaacccagg gtatcttccc tcagcagcct tccagccgag gaccagaaaa gctatgattc
     1741 cattagaaga cttctggagg tccccagatc tttggagtgt gggaatggaa gctgctttga
     1801 ggcaaaggct cataaaccct gcaagtcttc atggtcttct caccagcctt tccagcaggt
     1861 tctagtgcct tgacctgggg taggaccgag tgaaggagga agagggtaaa agggcacaga
     1921 cttccccagc tctgccctaa ataaaataac aatgctgatt caaaaaaaaa aaaaaaaa
//
