LOCUS       NM_026960               1776 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus gasdermin D (Gsdmd), mRNA.
ACCESSION   NM_026960
VERSION     NM_026960.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Wright,S.S., Kumari,P., Fraile-Agreda,V., Wang,C., Shivcharan,S.,
            Kappelhoff,S., Margheritis,E.G., Matz,A., Vasudevan,S.O., Rubio,I.,
            Bauer,M., Zhou,B., Vanaja,S.K., Cosentino,K., Ruan,J. and
            Rathinam,V.A.
  TITLE     Transplantation of gasdermin pores by extracellular vesicles
            propagates pyroptosis to bystander cells
  JOURNAL   Cell 188 (2), 280-291 (2025)
   PUBMED   39742811
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Jena,K.K., Mambu,J., Boehmer,D., Sposito,B., Millet,V., de Sousa
            Casal,J., Muendlein,H.I., Spreafico,R., Fenouil,R., Spinelli,L.,
            Wurbel,S., Riquier,C., Galland,F., Naquet,P., Chasson,L.,
            Elkins,M., Mitsialis,V., Ketelut-Carneiro,N., Bugda Gwilt,K.,
            Thiagarajah,J.R., Ruan,H.B., Lin,Z., Lien,E., Shao,F., Chou,J.,
            Poltorak,A., Ordovas-Montanes,J., Fitzgerald,K.A., Snapper,S.B.,
            Broggi,A. and Zanoni,I.
  TITLE     Type III interferons induce pyroptosis in gut epithelial cells and
            impair mucosal repair
  JOURNAL   Cell 187 (26), 7533-7550 (2024)
   PUBMED   39500322
REFERENCE   3  (bases 1 to 1776)
  AUTHORS   Grin,P.M., Baid,K., de Jesus,H.C.R., Kozarac,N., Bell,P.A.,
            Jiang,S.Z., Kappelhoff,R., Butler,G.S., Leborgne,N.G.F., Pan,C.,
            Pablos,I., Machado,Y., Vederas,J.C., Kim,H., Benarafa,C.,
            Banerjee,A. and Overall,C.M.
  TITLE     SARS-CoV-2 3CLpro (main protease) regulates caspase activation of
            gasdermin-D/E pores leading to secretion and extracellular activity
            of 3CLpro
  JOURNAL   Cell Rep 43 (12), 115080 (2024)
   PUBMED   39673710
REFERENCE   4  (bases 1 to 1776)
  AUTHORS   Wang,Y., Zhang,Y., Kim,K., Han,J., Okin,D., Jiang,Z., Yang,L.,
            Subramaniam,A., Means,T.K., Nestle,F.O., Fitzgerald,K.A.,
            Randolph,G.J., Lesser,C.F., Kagan,J.C., Mathis,D. and Benoist,C.
  TITLE     A pan-family screen of nuclear receptors in immunocytes reveals
            ligand-dependent inflammasome control
  JOURNAL   Immunity 57 (12), 2737-2754 (2024)
   PUBMED   39571575
REFERENCE   5  (bases 1 to 1776)
  AUTHORS   Chen,P., Zhang,Z., Sakai,L., Xu,Y., Wang,S., Lee,K.E., Geng,B.,
            Kim,J., Zhao,B., Wang,Q., Wen,H., Chandler,H.L. and Zhu,H.
  TITLE     Neutrophil pyroptosis regulates corneal wound healing and
            post-injury neovascularisation
  JOURNAL   Clin Transl Med 14 (11), e1762 (2024)
   PUBMED   39496510
REFERENCE   6  (bases 1 to 1776)
  AUTHORS   Shi,J., Zhao,Y., Wang,K., Shi,X., Wang,Y., Huang,H., Zhuang,Y.,
            Cai,T., Wang,F. and Shao,F.
  TITLE     Cleavage of GSDMD by inflammatory caspases determines pyroptotic
            cell death
  JOURNAL   Nature 526 (7575), 660-665 (2015)
   PUBMED   26375003
  REMARK    GeneRIF: identification of gasdermin D (Gsdmd) by genome-wide
            clustered regularly interspaced palindromic repeat (CRISPR)-Cas9
            nuclease screens of caspase-11- and caspase-1-mediated pyroptosis
            in mouse bone marrow macrophages
REFERENCE   7  (bases 1 to 1776)
  AUTHORS   Fujii,T., Tamura,M., Tanaka,S., Kato,Y., Yamamoto,H., Mizushina,Y.
            and Shiroishi,T.
  TITLE     Gasdermin D (Gsdmd) is dispensable for mouse intestinal epithelium
            development
  JOURNAL   Genesis 46 (8), 418-423 (2008)
   PUBMED   18693275
  REMARK    GeneRIF: This study clearly shows that Gsdmd is not essential for
            development of mouse intestinal tract or epithelial cell
            differentiation.
REFERENCE   8  (bases 1 to 1776)
  AUTHORS   Tamura,M., Tanaka,S., Fujii,T., Aoki,A., Komiyama,H., Ezawa,K.,
            Sumiyama,K., Sagai,T. and Shiroishi,T.
  TITLE     Members of a novel gene family, Gsdm, are expressed exclusively in
            the epithelium of the skin and gastrointestinal tract in a highly
            tissue-specific manner
  JOURNAL   Genomics 89 (5), 618-629 (2007)
   PUBMED   17350798
REFERENCE   9  (bases 1 to 1776)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of human DFNA5L, mouse Dfna5l,
            and rat Dfna5l genes in silico
  JOURNAL   Int J Oncol 25 (3), 765-770 (2004)
   PUBMED   15289881
REFERENCE   10 (bases 1 to 1776)
  AUTHORS   Leimeister,C., Bach,A., Woolf,A.S. and Gessler,M.
  TITLE     Screen for genes regulated during early kidney morphogenesis
  JOURNAL   Dev Genet 24 (3-4), 273-283 (1999)
   PUBMED   10322635
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC116520.14.
            
            On Oct 19, 2007 this sequence version replaced NM_026960.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165858.1, AK007710.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-143               AC116520.14        51184-51326
            144-367             AC116520.14        52250-52473
            368-560             AC116520.14        53089-53281
            561-729             AC116520.14        53794-53962
            730-832             AC116520.14        54268-54370
            833-883             AC116520.14        54605-54655
            884-973             AC116520.14        54932-55021
            974-1149            AC116520.14        55141-55316
            1150-1294           AC116520.14        55391-55535
            1295-1368           AC116520.14        55635-55708
            1369-1776           AC116520.14        55842-56249
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.0 cM"
     gene            1..1776
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /note="gasdermin D"
                     /db_xref="GeneID:69146"
                     /db_xref="MGI:MGI:1916396"
     exon            1..143
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            144..367
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..1611
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /note="gasdermin domain containing 1; gasdermin
                     domain-containing protein 1; gasderminD-1"
                     /codon_start=1
                     /product="gasdermin-D"
                     /protein_id="NP_081236.1"
                     /db_xref="CCDS:CCDS27551.1"
                     /db_xref="GeneID:69146"
                     /db_xref="MGI:MGI:1916396"
                     /translation="MPSAFEKVVKNVIKEVSGSRGDLIPVDSLRNSTSFRPYCLLNRK
                     FSSSRFWKPRYSCVNLSIKDILEPSAPEPEPECFGSFKVSDVVDGNIQGRVMLSGMGE
                     GKISGGAAVSDSSSASMNVCILRVTQKTWETMQHERHLQQPENKILQQLRSRGDDLFV
                     VTEVLQTKEEVQITEVHSQEGSGQFTLPGALCLKGEGKGHQSRKKMVTIPAGSILAFR
                     VAQLLIGSKWDILLVSDEKQRTFEPSSGDRKAVGQRHHGLNVLAALCSIGKQLSLLSD
                     GIDEEELIEAADFQGLYAEVKACSSELESLEMELRQQILVNIGKILQDQPSMEALEAS
                     LGQGLCSGGQVEPLDGPAGCILECLVLDSGELVPELAAPIFYLLGALAVLSETQQQLL
                     AKALETTVLSKQLELVKHVLEQSTPWQEQSSVSLPTVLLGDCWDEKNPTWVLLEECGL
                     RLQVESPQVHWEPTSLIPTSALYASLFLLSSLGQKPC"
     exon            368..560
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            561..729
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            730..832
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            833..883
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            884..973
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1149
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1294
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1368
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1776
                     /gene="Gsdmd"
                     /gene_synonym="1810036L03Rik; DF5L; Dfna5l; GsdmD-1;
                     Gsdmdc1; M2-4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgtcctgcc gcctgagttc cgctcttggt cgtggctccc gttgctcccg ggttgagcag
       61 acaatagacc cctccccggc atcccagcag gtcctcgctt cgcttggtgg acccagatac
      121 ctcggcaggg gtgaaaaatc gaggaccatg ccatcggcct ttgagaaagt ggtcaagaat
      181 gtgatcaagg aggtaagcgg cagcagaggc gatctcattc cggtggacag cctgcggaac
      241 tccaccagct tcaggcccta ctgccttctg aacaggaaat tttcaagctc aaggttctgg
      301 aaaccccgtt attcatgtgt caacctgtca atcaaggaca tcctggagcc cagtgctcca
      361 gaaccagaac cggagtgttt tggctccttc aaagtctctg atgtcgtcga tgggaacatt
      421 cagggcagag tgatgttgtc aggcatggga gaagggaaaa tttctggtgg ggctgcagtg
      481 tctgacagtt ccagtgcctc catgaatgtg tgtatactgc gtgtgactca gaagacctgg
      541 gagaccatgc agcatgaaag gcaccttcag cagcctgaga acaaaatcct gcaacagctt
      601 cggagtcgtg gggatgacct gtttgtggtg accgaggtgc tgcagacaaa ggaggaagtg
      661 cagatcactg aggtccacag ccaagagggc tcaggccagt ttacgctgcc tggagcttta
      721 tgcttgaagg gtgaaggcaa gggccaccaa agccggaaga agatggtgac cattcctgca
      781 ggcagcatcc tggcattccg agtggcccaa ctgcttattg gctctaaatg ggatatcctt
      841 ctcgtctcag atgagaaaca gaggaccttt gagccctcct caggtgacag aaaagcagtg
      901 ggccagaggc accatggcct caatgtgctt gctgcgcttt gttccatcgg aaagcagctc
      961 agtctcctgt cagatgggat tgatgaggag gaattaattg aggcggcaga cttccagggc
     1021 ctgtatgctg aggtgaaggc ttgctcctca gaactggaga gcttggaaat ggagttgaga
     1081 caacagatac tggtgaacat cggaaagatt ttacaggacc agcccagcat ggaagcctta
     1141 gaggcctcac tagggcaggg cctgtgcagt ggcggccagg tggagcctct ggacggccca
     1201 gctggctgca tccttgagtg tctggtgctt gactctggag aactggtgcc ggaactcgca
     1261 gcccctatct tctacctgct gggagcactg gctgtgctga gtgaaaccca gcagcagctg
     1321 ctagctaagg ctctggagac aacggtgctg tcaaagcagc tggagttggt gaagcacgtc
     1381 ttggaacaga gcaccccgtg gcaggagcag agttctgtgt ccctgcccac cgtgctcctt
     1441 ggggactgct gggatgaaaa gaatcccacc tgggtcttgc tagaagaatg tggcctaagg
     1501 ctgcaggtag aatcccccca ggtgcactgg gaaccaacgt ctctgatccc cacaagtgcg
     1561 ctctatgcct ccctgttcct attgtcaagt ctaggccaga aaccttgtta gcctgtgggc
     1621 ctcccttccc acaacatctc catgtcctac cctccagcca aggtagaatc ttgccaagcc
     1681 tagcctttgg gaagccaaga accatactca gtcacagggt tataatgcac tgagatccag
     1741 aagttgggaa aactcaataa atgtacaaag gaaagc
//
