LOCUS       NM_175437               3740 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus gamma-secretase activating protein (Gsap), transcript
            variant 1, mRNA.
ACCESSION   NM_175437
VERSION     NM_175437.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Xu,P., Chang,J.C., Zhou,X., Wang,W., Bamkole,M., Wong,E.,
            Bettayeb,K., Jiang,L.L., Huang,T., Luo,W., Xu,H., Nairn,A.C.,
            Flajolet,M., Ip,N.Y., Li,Y.M. and Greengard,P.
  TITLE     GSAP regulates lipid homeostasis and mitochondrial function
            associated with Alzheimer's disease
  JOURNAL   J Exp Med 218 (8) (2021)
   PUBMED   34156424
  REMARK    GeneRIF: GSAP regulates lipid homeostasis and mitochondrial
            function associated with Alzheimer's disease.
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Chu,J., Li,J.G., Hoffman,N.E., Stough,A.M., Madesh,M. and
            Pratico,D.
  TITLE     Regulation of gamma-secretase activating protein by the
            5Lipoxygenase: in vitro and in vivo evidence
  JOURNAL   Sci Rep 5, 11086 (2015)
   PUBMED   26076991
  REMARK    GeneRIF: GSAP cleavage via caspase-3 is regulated and depend upon
            the availability of 5-Lipoxygenase in Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3740)
  AUTHORS   Hussain,I., Fabregue,J., Anderes,L., Ousson,S., Borlat,F.,
            Eligert,V., Berger,S., Dimitrov,M., Alattia,J.R., Fraering,P.C. and
            Beher,D.
  TITLE     The role of gamma-secretase activating protein (GSAP) and imatinib
            in the regulation of gamma-secretase activity and amyloid-beta
            generation
  JOURNAL   J Biol Chem 288 (4), 2521-2531 (2013)
   PUBMED   23209290
  REMARK    GeneRIF: gamma-secretase activating protein (GSAP) and imatinib
            have roles in the regulation of gamma-secretase activity and
            amyloid-beta generation
REFERENCE   4  (bases 1 to 3740)
  AUTHORS   He,G., Luo,W., Li,P., Remmers,C., Netzer,W.J., Hendrick,J.,
            Bettayeb,K., Flajolet,M., Gorelick,F., Wennogle,L.P. and
            Greengard,P.
  TITLE     Gamma-secretase activating protein is a therapeutic target for
            Alzheimer's disease
  JOURNAL   Nature 467 (7311), 95-98 (2010)
   PUBMED   20811458
  REMARK    GeneRIF: Study reports discovery of a novel gamma-secretase
            activating protein (GSAP) that selectively increases amyloid-beta
            production through interactions with gamma-secretase and its
            substrate, the amyloid precursor protein carboxy-terminal fragment.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113052.10.
            
            On Feb 17, 2022 this sequence version replaced NM_175437.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033694.1, AK170515.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC113052.10        34237-34401
            166-242             AC113052.10        42342-42418
            243-299             AC113052.10        43987-44043
            300-369             AC113052.10        55183-55252
            370-423             AC113052.10        55530-55583
            424-512             AC113052.10        65332-65420
            513-582             AC113052.10        69168-69237
            583-632             AC113052.10        70332-70381
            633-737             AC113052.10        74186-74290
            738-797             AC113052.10        74716-74775
            798-841             AC113052.10        75966-76009
            842-927             AC113052.10        77103-77188
            928-1005            AC113052.10        90747-90824
            1006-1107           AC113052.10        94841-94942
            1108-1200           AC113052.10        98683-98775
            1201-1418           AC113052.10        98881-99098
            1419-1488           AC113052.10        101312-101381
            1489-1571           AC113052.10        101901-101983
            1572-1625           AC113052.10        105812-105865
            1626-1754           AC113052.10        117844-117972
            1755-1813           AC113052.10        118498-118556
            1814-1845           AC113052.10        119159-119190
            1846-1907           AC113052.10        120924-120985
            1908-2003           AC113052.10        125987-126082
            2004-2074           AC113052.10        128938-129008
            2075-2169           AC113052.10        129450-129544
            2170-2289           AC113052.10        135619-135738
            2290-2351           AC113052.10        136530-136591
            2352-2453           AC113052.10        137731-137832
            2454-2553           AC113052.10        137987-138086
            2554-3740           AC113052.10        138480-139666
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 9.83 cM"
     gene            1..3740
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /note="gamma-secretase activating protein"
                     /db_xref="GeneID:212167"
                     /db_xref="MGI:MGI:2442259"
     exon            1..165
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..2645
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein pigeon homolog"
                     /codon_start=1
                     /product="gamma-secretase-activating protein isoform 1"
                     /protein_id="NP_780646.2"
                     /db_xref="CCDS:CCDS39021.1"
                     /db_xref="GeneID:212167"
                     /db_xref="MGI:MGI:2442259"
                     /translation="MALRLVTHFDVLEDVLPSLLTQAATTDEGDRAGVLETTYGSLRV
                     LNIERNGNIIYTYKDNKGNAVFGLYDCQTRQNEHLYTFEKDMQAVSCSVNSERTVLAA
                     SFIQYTTEGVKNDLQPGSKCLTLLVEIHPVNNVKVLKAVDSCVWVQFLYPQAESHLLP
                     QNHLLLISEEKYIERFHIQITREDGDRVVIRNSSHLPRDRLAEDFVWAQWDLSEQRLY
                     YIELKESRSILKCIQFRADESFNLMFEMPLDITLTGLRFKLVNFGYDYRQDREKLCNQ
                     PSLCIFTNHTGSLCMCYSPKSDSREEITYSVFYLHKGYRKIFTAAPGSADSQVTNGAD
                     SQVTDGIAFLNLGYFVAVYSPGHFLHLLNIQHPDLVCHSLFLTGNNKIAAVLPPSPLQ
                     SLPGSLVLDCYSGKVYRVTLDQSYLLRFLWNAHLDCERMAALHCILSCSQDPGFPEEQ
                     IIQWISEHVSACHSFDLIQEFLIASSYWSVYAELDDMGMLLQYSSVLTWNTEIPGIKF
                     TTEELPLPLMKVYGLKGYWAKLNSNLEYIKYTKPHLHYHNSVVRREWHNLISEERTGK
                     RRSTMYVRNILENAMKVIASMETRTLEPRLIPFLQEEDRHQRLLMGLMVSELRDHLLR
                     HLQGVEKKKIEQMVLDYISKLLDLIWCLLETSWRKHSMHPLVLHLNSHCSAADFEVFH
                     LMTRILDAASSLCLPLPPGFHSLHTILGVHCLPLYSLLHYIDNGVLLLTETAVTRLMK
                     DLDNSEKNEQLKFSIIVRLPPLIGQKVCRLWDHPMSSNIISRNHVARLLKNYRKEPRN
                     SMIDKSSFPVEFLPLNYFIEILMGLESSNQALYGFEGHDNVDAEFVEEAALKHTTMLL
                     GL"
     exon            166..242
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            243..299
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            300..369
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            370..423
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            424..512
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            513..582
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            583..632
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            633..737
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            738..797
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            798..841
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            842..927
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1005
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1107
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1200
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1418
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1488
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1571
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1625
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1754
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1813
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1845
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1907
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2003
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2074
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2169
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2289
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2351
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2453
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2553
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     exon            2554..3740
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3185..3190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /note="hexamer: ATTAAA"
     polyA_site      3210
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
     regulatory      3716..3721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /note="hexamer: AATAAA"
     polyA_site      3740
                     /gene="Gsap"
                     /gene_synonym="A530088I07Rik; Pion"
                     /note="major polyA site"
ORIGIN      
        1 aggagaggtg gttgtaggct gagaggtgta agcccgcccg gaatgcccgc cgctgctgct
       61 tgggagccat ggctctgcgg ctggtcactc acttcgatgt gctggaggac gtgctcccga
      121 gtctgctcac acaggcggcg acaacggacg aaggagacag agcaggtgtt ttagaaacaa
      181 cttatgggag cttacgtgta ctaaacatcg aaagaaatgg aaacattatt tatacctata
      241 aggacaataa gggaaacgct gtttttggat tatatgattg ccaaaccaga cagaatgagc
      301 atctgtacac ctttgagaag gacatgcaag ctgtcagttg ttcagtcaac agtgagagaa
      361 cagtgcttgc tgcgagtttc attcagtata ccacagaggg agtaaagaat gaccttcagc
      421 caggatcgaa gtgcctaact ttgctggtag aaatacaccc tgttaacaac gtgaaggttc
      481 tgaaagcagt ggatagctgt gtttgggtac agttcctcta cccacaagct gaaagtcatc
      541 ttcttccaca gaaccacctg ctactgatct cagaagagaa atatattgag cggtttcata
      601 tccagatcac cagagaagat ggagacagag tggtgattag aaattctagc catctcccaa
      661 gagacagact agctgaagac tttgtttggg ctcagtggga tctgtcagag cagagactat
      721 actacattga actaaaggag tcaaggagta tcttgaaatg catccagttt agggcagatg
      781 aaagttttaa cttaatgttt gaaatgccct tggacataac gttaactggc ttgagattta
      841 aacttgtcaa ctttggatat gactatcgtc aagaccgtga gaagctgtgc aatcagcctt
      901 ctctctgcat ttttaccaac catacaggga gtttgtgtat gtgttacagc cctaagtctg
      961 actcacggga agaaatcaca tattcagtgt tctacctcca taaaggatac agaaagatct
     1021 tcactgcagc tcctggaagt gctgactcac aggtcacaaa cggtgctgac tcacaggtca
     1081 cagacggcat cgctttcctc aaccttggct actttgtggc tgtttactcc cctggccatt
     1141 tccttcattt acttaacatt cagcatccag acttggtctg ccacagtctg tttctgacag
     1201 gaaacaacaa aatagctgct gtgctacctc caagtccttt gcagtccctg ccggggtccc
     1261 tggttctgga ctgctattct ggaaaggtct atagagtaac ccttgaccag tcatacttgt
     1321 tgcggttcct atggaacgcc catctcgact gtgagagaat ggctgcactg cactgtatac
     1381 tatcctgtag tcaagaccca ggtttcccgg aagaacagat tatccagtgg atttcggagc
     1441 atgtctctgc ttgccattca tttgacctca ttcaagaatt tctaattgct tcctcctatt
     1501 ggagtgtgta tgcagagctg gacgacatgg gcatgctcct acaatactcc tctgtgctga
     1561 cttggaacac agaaattccc ggaataaagt ttacgacaga ggaactccca ttgcctctta
     1621 tgaaggtcta cggcttgaag gggtactggg caaagctgaa ctccaacctt gagtacatta
     1681 agtatacaaa acctcacttg cactatcaca acagcgtcgt gaggagggaa tggcataacc
     1741 tgatctctga agagagaacg ggcaaaagga gatccacaat gtacgtgagg aacattcttg
     1801 aaaacgcaat gaaggtgatt gctagcatgg aaacgaggac actggagcca agattaatac
     1861 cattcttaca agaggaagac cggcaccaga ggctgctcat gggcttgatg gtgtctgagc
     1921 taagagacca cctactgaga cacctgcagg gtgtagaaaa gaagaaaatt gaacagatgg
     1981 ttctggacta catttcaaaa ctgctggatc tcatttggtg cttgctggaa accagttgga
     2041 ggaaacacag tatgcatcca ttggttctcc acctcaatag ccattgcagt gctgcagatt
     2101 ttgaagtctt tcatctcatg accaggattt tggatgctgc tagcagtttg tgtttgcctc
     2161 tgcctcctgg tttccactct ctgcacacga tccttggggt ccactgcctc cctttgtaca
     2221 gccttctgca ctacattgat aacggagtgc tgctgcttac tgagaccgcg gtcacgaggc
     2281 ttatgaaaga tctggacaat tcagagaaaa atgaacaact gaaattcagc atcattgtcc
     2341 ggctccctcc gcttattggc cagaaggtct gccggctttg ggaccacccc atgagttcca
     2401 atatcatttc acggaaccat gtggcccggc tgctgaaaaa ctacaggaaa gagcctcgga
     2461 actccatgat tgacaaatca tcatttccgg tagaatttct gcctctgaac tacttcatcg
     2521 aaattctgat gggcctggag tcctccaacc aagcactgta tggctttgaa ggacatgaca
     2581 atgtggacgc agaatttgta gaggaagctg ctctgaaaca caccaccatg cttttgggct
     2641 tatgaaagtg gacgtgcaga cggacgattt caatattttc acctcccttt tctggaaact
     2701 ttcccactgt ctttaagatt gggaacaggt cagaatatac tgcagagctg ttggcttcgg
     2761 tacagttctt attccgaggc agctgctcct tctctcagtt caagcagcca gtaaacagcc
     2821 aagaggaaag aacaagttca aggccgtgtg ctgcggcagg aacagtgccc tccttggtgg
     2881 cccagtaggc ctggagctgg ttctgttgca ctaaagaatc taatacaaac acaggggaca
     2941 atgcctagaa cagtgagagc caggcggtca ctggaaagga cataggacag gcaggaaagg
     3001 tcagtgctgg gaagagcatc tcaggagaac ctgaaactga ggaagcaaag cctgtcacgt
     3061 ccagtttgga ggaactggac aaataaccat ttgtccatac agggcttaga ccagcctttg
     3121 cctttgtggt ggctcagata gagccatatt taattttata tttatgattt agtactatct
     3181 tgtcattaaa taccacctca aatactgaaa cctttggtct gactctccct aagttttggg
     3241 tatagcctta tttgcataag gcatcttgag cccgtcagtg tgactaggca gaggcccctg
     3301 acacctgatg aggtgttcgt tcggtccttt ctcatctccc tcctgcagtg acatcatgct
     3361 gacccctaag gctcggcttt ccaggttccg ttctgcttct ctgtcctcat gggttcctgt
     3421 tcacatggcg agattttaat gattctacat gtcactaact cacatgaaat ccaccgtatt
     3481 ttcagcaatc tttgctgtgc tctagaaact gtcctagcct atagaatttc ccttttgctc
     3541 caaatagtca gccaggctcg gaaccttgcc accaagtcag caatgctgca atacggtatt
     3601 ttcacttttt tttttttttc tgtctgttca tattgcttgt gttacaaatt actctgtact
     3661 ggagacagca ggttttattt agttcaaatt aaatttttaa aaatcctttt ttgctaataa
     3721 aactctgaca gtacacttaa
//
