LOCUS       NM_021296               4065 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus GrpE-like 2, mitochondrial (Grpel2), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_021296
VERSION     NM_021296.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Yang,R., Zhang,X., Zhang,Y., Wang,Y., Li,M., Meng,Y., Wang,J.,
            Wen,X., Yu,J. and Chang,P.
  TITLE     Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy
            through DLST-mediated mitochondrial dysfunction: a proof-of-concept
            study
  JOURNAL   J Transl Med 21 (1), 200 (2023)
   PUBMED   36927450
  REMARK    GeneRIF: Grpel2 maintains cardiomyocyte survival in diabetic
            cardiomyopathy through DLST-mediated mitochondrial dysfunction: a
            proof-of-concept study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   3  (bases 1 to 4065)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   4  (bases 1 to 4065)
  AUTHORS   Naylor,D.J., Stines,A.P., Hoogenraad,N.J. and Hoj,P.B.
  TITLE     Evidence for the existence of distinct mammalian cytosolic,
            microsomal, and two mitochondrial GrpE-like proteins, the
            Co-chaperones of specific Hsp70 members
  JOURNAL   J Biol Chem 273 (33), 21169-21177 (1998)
   PUBMED   9694873
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY249944.1, AK145493.1 and BC094583.1.
            
            On Feb 11, 2006 this sequence version replaced NM_021296.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068232.1, AK145493.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BY249944.1         27-34
            9-3379              AK145493.1         1-3371
            3380-4065           BC094583.1         3347-4032
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 34.69 cM"
     gene            1..4065
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="GrpE-like 2, mitochondrial"
                     /db_xref="GeneID:17714"
                     /db_xref="MGI:MGI:1334416"
     exon            1..106
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..707
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="grpE protein homolog 2, mitochondrial"
                     /codon_start=1
                     /product="grpE protein homolog 2, mitochondrial precursor"
                     /protein_id="NP_067271.1"
                     /db_xref="CCDS:CCDS29288.1"
                     /db_xref="GeneID:17714"
                     /db_xref="MGI:MGI:1334416"
                     /translation="MAARSLWAVQRLQRLLASGAMSESRGWLHPFSTATQRTAGEDCS
                     SEDPPDGLGPSLAEQALRLKAVKLEKEVQDLTLRYQRAVADCENIRRRTQRCVEDAKI
                     FGIQSFCKDLVEVADILEKTAKCCSEGAEPEDHRRTLEKVFQGLSLLEARLKSVFTKH
                     GLEKMTPIGDKYDPHEHELICHMPAGVGVQPGTVALVRQDGYKLHGRTIRLAQVEVAV
                     ESQRRL"
     exon            107..260
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /inference="alignment:Splign:2.1.0"
     exon            261..342
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /inference="alignment:Splign:2.1.0"
     exon            343..4046
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1438..1443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="hexamer: AACAAA"
     polyA_site      1459
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="major polyA site"
     regulatory      4028..4033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="hexamer: ATTAAA"
     polyA_site      4046
                     /gene="Grpel2"
                     /gene_synonym="mt-GrpE#2; mt-Grpel2"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgtag tgttggcgct ctactctgga acatggcggc gcgttcgctg tgggcggtgc
       61 agcggctgca gcgcctcctg gcctcggggg ctatgtcaga gagcagggga tggctccacc
      121 cattcagcac tgccacccaa agaactgctg gggaagactg cagttccgag gaccctcctg
      181 atggactggg accctccctt gctgagcagg ccttaaggct caaagctgtt aaactggaaa
      241 aggaagtcca ggatttaacc ctgagatacc agagagctgt cgctgattgt gaaaacataa
      301 ggagaagaac ccagagatgt gtggaagacg cgaagatatt tgggatccag agcttctgca
      361 aggacctggt ggaggtggca gacattttgg agaagactgc caagtgctgt tcagaagggg
      421 cagaacctga ggaccacagg cgcactctgg aaaaagtctt ccaagggctg tccctgctag
      481 aagccagact gaaaagcgta tttaccaagc atggcctaga gaaaatgaca cccatcggtg
      541 acaaatacga ccctcacgag catgaactca tctgtcacat gccagcaggt gttggggtac
      601 agcctggcac cgtggcatta gttcggcaag atggctacaa acttcatggc cgcaccatta
      661 gacttgcgca agtggaagtg gcagtagagt ctcagagaag actctgaaca ggtcactgga
      721 accaaacctt ctcccagagt caggcaccta tgtctccttt atttattaag ctggtttgta
      781 ttatacacga gtccttcgtg tgctccgttt cggatttagt cacattggct gtatctctac
      841 agcgtcttat tgggtttgtg tgacctgttt ggtctcatcg ggagtcctgc cactaggcat
      901 ttgaacaata ggacacgtgt cgtggccttt atcaaaatgt ttacaaaaat catttaaatt
      961 ggtactctga tgattgaatc caaaatcttc ttacctgtgt tttcatctaa ctgctggaat
     1021 tagtgcagca ccttgtatct tatattttgg gaagagggaa aaggtaggga ttggtttgac
     1081 gtttaggtac gtagatgacg atgccttacc cttgtatatg tttaagtcat gtgggctggc
     1141 gtgtagagag atttcctaag actcctatct catgcttgta gtccttgcca gggtcacact
     1201 cacacccctt tctggtccct tttggaaggg ctaaagggtc aggccagcca gtcttccttt
     1261 cttgttggta aggtgaatat atttattact aaactggtct cgagtctaag aacaggcaag
     1321 ccctggttgc cgtgacaata caactcaatc ccagagagac taaaagcctc catacatctc
     1381 aagttggaga aatagacttt ggggacttgg catgatggct gtcaattcaa aaataaaaac
     1441 aaaatattaa ggtggataat attttttaca gtttataatt tacacaaaag taattgcctc
     1501 ccttgcatgg actcagaatg aaccaggcta tcctcctttc tgggttggag aggacaagag
     1561 acttcatagc agcaacaaca aaaatgaata caatcagagt cctcgggcgc attgtaaagc
     1621 agttgctgtc accttcacca ttccagtttt taaatcctga gtcaaaagca ccaagaaaaa
     1681 gaaatttttt tgtatattta ttattgacaa aaagaccaaa gttctctcag ttctgcatat
     1741 gttttgttaa gaaaaggtgt ggtatagctc caaccctgtc cacctaaaat catttgcagt
     1801 gcacagagga agggccatat tccttgtgtt cttccttggt gaactgtatg tatctcctgg
     1861 aagatggaca gtaaggctaa ggtgcacctg tccatccaca cttgtactgg gggagcaagg
     1921 atgttgattt tcatggtcct ggaagccaga ggagtgatct gcatcttgta ggaaccagtg
     1981 cctgagttca tggggtcacc accagacaga cagcactggg ttggcactga agtctttgct
     2041 gacattgaca tcacacttag agatggaatg gaaggtaagt tcatcgtatc ccacacccag
     2101 aaagaaaggt ggaagaaccc ctgggggcag cagagttggt tcttgccagt aacaatgacc
     2161 tggccatcgg gcagctccta actcttctcc agggagaagg atgcagcagc aggcatgtct
     2221 tatgggaagt cccaggcaac atggcacagg ttccccttga tgttaacagt tggccgtggt
     2281 gaggctgtag cttgctcagg agcttcatga gtagtctaga aggtcttgtc caacaggatc
     2341 acataaggaa ggatatagtc cctgtagatg agcaatgtgg gtgaccctgt ctccagagtc
     2401 cattgggatg caagtggtac tatgaagaca gcacagcctt ggtggctatg tgcatggctg
     2461 ggtgttgatg atctgggtca tctattcatg actggcttag ggttcgggga gcagcaccaa
     2521 gcgctcctac gggaccacac atgtcacata gtataaagta tggtggtgtg tcttgtcatt
     2581 caagttggtg acagttaaat cctccatgga tacctcaggg tcaggactct tctcttgctc
     2641 taggccttgt tacccaccca tgtaggaaac atggctaacg ccaccatcac accccaatac
     2701 attgggctac cccaccatga agcaggagac agacttggaa gcatgctcac ctatgaagcc
     2761 agctctgtta gtgccagtga aacaatatct gcctccatgg caattggtga caggtggacc
     2821 cggcagcgga ggatctgaga gaagcctgtg cttgccaggt aaatgtggat accacagagc
     2881 atcttccctt ctacgtaagg agctattttt acagctcctt gggtatgaag caattttagg
     2941 cagcgccaac agcaaatgac ccaaataaca ttcgtatagc taattaccta cagtggctgg
     3001 ttctaccagc cctgattctt ggccttcgtc agattggctc tgggatccta tattagatta
     3061 tatggggaga aaacaaaaat tctggactct taacaatttc tcattaaata aggctgatgc
     3121 gtgccttttt tccccgtcat aggggctgac aagttactac ctctcacaac aacaaaactt
     3181 gggtttttag agcattgggg attggatgca aggtctcatg aatgccaggt aagctctcta
     3241 cagctcccag ccctggcaag ctttgtttcc tcgtttctgt atccgcctta ggccttgttt
     3301 gtatgtacat ccacttatgt attatccttg taaattctat gaacccgtat agtttaagtc
     3361 tcaataactg atgatgtgta aaagatttgc ccagtaaata ccttaatgcg cgaataatac
     3421 acaaggtaaa cttgataaag gttttagcat aagaggtctt atataggaga aaatgggaat
     3481 gagaaattga tgtaaataaa cttgcttagg aaaaatacta ggtagaattt ggcaaaagca
     3541 gccgtctgaa tctcttagca taattctgcc aggcaggcag gacggcctgg gttaaaggat
     3601 ctttcccttt attaatctct taagagatgg agtgacttct ccctgggaag gtctatggat
     3661 tctataacat cacccattct gctgcttctg gagcttaaag atgtctattc taggactaga
     3721 ctcagagaca gcccttctac agactttgac tggactacaa attaaactgg caccaactgg
     3781 gtcactagaa gaaccaaaat ctgttgtcca cataaataca agaatcccac aaaatctgag
     3841 actgtcagaa aattggtgct tagatcccac cctcatccac aggacagggc ttaggtctcc
     3901 tagaaactgg aagagcagag aaagctataa tgaataaagg tggtcttgta gacattgtct
     3961 gggaggccct ttgatacata aaactggaaa ttgtaaatat acaggtatac agattttcaa
     4021 cttctgtatt aaaaaagaga aaaacaaaaa aaaaaaaaaa aaaaa
//
