LOCUS       NM_001286065            2912 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus G protein-coupled receptor kinase 6 (Grk6), transcript
            variant 6, mRNA.
ACCESSION   NM_001286065
VERSION     NM_001286065.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Glaser,K.M., Tarrant,T.K. and Lammermann,T.
  TITLE     Combinatorial depletions of G-protein coupled receptor kinases in
            immune cells identify pleiotropic and cell type-specific functions
  JOURNAL   Front Immunol 13, 1039803 (2022)
   PUBMED   36451830
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Varney,M.J., Steyaert,W., Coucke,P.J., Delanghe,J.R., Uehling,D.E.,
            Joseph,B., Marcellus,R., Al-Awar,R. and Benovic,J.L.
  TITLE     G protein-coupled receptor kinase 6 (GRK6) regulates insulin
            processing and secretion via effects on proinsulin conversion to
            insulin
  JOURNAL   J Biol Chem 298 (10), 102421 (2022)
   PUBMED   36030052
  REMARK    GeneRIF: G protein-coupled receptor kinase 6 (GRK6) regulates
            insulin processing and secretion via effects on proinsulin
            conversion to insulin.
REFERENCE   3  (bases 1 to 2912)
  AUTHORS   Chaudhary,P.K., Kim,S., Jee,Y., Lee,S.H., Park,K.M. and Kim,S.
  TITLE     Role of GRK6 in the Regulation of Platelet Activation through
            Selective G Protein-Coupled Receptor (GPCR) Desensitization
  JOURNAL   Int J Mol Sci 21 (11), 3932 (2020)
   PUBMED   32486261
  REMARK    GeneRIF: Role of GRK6 in the Regulation of Platelet Activation
            through Selective G Protein-Coupled Receptor (GPCR)
            Desensitization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2912)
  AUTHORS   Wu,W., Sung,C.C., Yu,P., Li,J. and Chung,K.K.K.
  TITLE     S-Nitrosylation of G protein-coupled receptor kinase 6 and Casein
            kinase 2 alpha modulates their kinase activity toward
            alpha-synuclein phosphorylation in an animal model of Parkinson's
            disease
  JOURNAL   PLoS One 15 (4), e0232019 (2020)
   PUBMED   32343709
  REMARK    Erratum:[PLoS One. 2020 Jun 18;15(6):e0235296. doi:
            10.1371/journal.pone.0235296.. PMID: 32555609]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2912)
  AUTHORS   Serafin,D.S., Allyn,B., Sassano,M.F., Timoshchenko,R.G., Mattox,D.,
            Brozowski,J.M., Siderovski,D.P., Truong,Y.K., Esserman,D.,
            Tarrant,T.K. and Billard,M.J.
  TITLE     Chemerin-activated functions of CMKLR1 are regulated by G
            protein-coupled receptor kinase 6 (GRK6) and beta-arrestin 2 in
            inflammatory macrophages
  JOURNAL   Mol Immunol 106, 12-21 (2019)
   PUBMED   30576947
  REMARK    GeneRIF: findings show that chemerin-activated CMKLR1 regulation in
            inflammatory macrophages is largely GRK6 and beta-arrestin
            mediated, which may impact innate immunity and have therapeutic
            implications in rheumatic disease.
REFERENCE   6  (bases 1 to 2912)
  AUTHORS   Fong,A.M., Premont,R.T., Richardson,R.M., Yu,Y.R., Lefkowitz,R.J.
            and Patel,D.D.
  TITLE     Defective lymphocyte chemotaxis in beta-arrestin2- and
            GRK6-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 99 (11), 7478-7483 (2002)
   PUBMED   12032308
  REMARK    GeneRIF: deficiency causes defective lymphocyte chemotaxis
REFERENCE   7  (bases 1 to 2912)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   8  (bases 1 to 2912)
  AUTHORS   Premont,R.T., Macrae,A.D., Aparicio,S.A., Kendall,H.E., Welch,J.E.
            and Lefkowitz,R.J.
  TITLE     The GRK4 subfamily of G protein-coupled receptor kinases.
            Alternative splicing, gene organization, and sequence conservation
  JOURNAL   J Biol Chem 274 (41), 29381-29389 (1999)
   PUBMED   10506199
REFERENCE   9  (bases 1 to 2912)
  AUTHORS   Moepps,B., Vatter,P., Frodl,R., Waechter,F., Dixkens,C.,
            Hameister,H. and Gierschik,P.
  TITLE     Alternative splicing produces transcripts encoding four variants of
            mouse G-protein-coupled receptor kinase 6
  JOURNAL   Genomics 60 (2), 199-209 (1999)
   PUBMED   10486211
REFERENCE   10 (bases 1 to 2912)
  AUTHORS   Hall,R.A., Spurney,R.F., Premont,R.T., Rahman,N., Blitzer,J.T.,
            Pitcher,J.A. and Lefkowitz,R.J.
  TITLE     G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+)
            exchanger regulatory factor via a PDZ domain-mediated interaction
  JOURNAL   J Biol Chem 274 (34), 24328-24334 (1999)
   PUBMED   10446210
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009762.8, Y15799.1, AK051405.1 and AW048460.1.
            
            Transcript Variant: This variant (6) uses an alternate in-frame
            splice site in the central coding region, and uses an alternate
            splice site in the 3' coding region, which results in a frameshift,
            compared to variant 1. The encoded isoform (f) is shorter and has a
            distinct C-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146119.1, SRR13422590.194757.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               CT009762.8         87287-87459         c
            174-916             Y15799.1           1-743
            917-1877            Y15799.1           846-1806
            1878-2892           AK051405.1         1964-2978
            2893-2912           AW048460.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.06 cM"
     gene            1..2912
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="G protein-coupled receptor kinase 6"
                     /db_xref="GeneID:26385"
                     /db_xref="MGI:MGI:1347078"
     exon            1..230
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1807
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /EC_number="2.7.11.16"
                     /note="isoform f is encoded by transcript variant 6; g
                     protein-coupled receptor kinase GRK6"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 6 isoform f"
                     /protein_id="NP_001272994.1"
                     /db_xref="CCDS:CCDS88458.1"
                     /db_xref="GeneID:26385"
                     /db_xref="MGI:MGI:1347078"
                     /translation="MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEE
                     LRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCTAFLDGVSEYEVTPDEKRKACG
                     RRLMQNFLSHTGPDLIPEVPRQLVSNCAQRLEQGPCKDLFQELTRLTHEYLSTAPFAD
                     YLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLE
                     KKRIKKRKGEAMALNEKQILEKVNSRFVAGFPEARAVFYAAEICCGLEDLHRERIVYR
                     DLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVRNERYTFSPDW
                     WALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVAEEYTDRFSSQARSLCSQLLSK
                     DPAERLGCRGGGAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFS
                     TVKGVDLEPTDQDFYQKFATGSVSIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQ
                     PTAPPKKGLLQRLFSRQDCCGNCSDSEEELPTRL"
     exon            231..326
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            327..439
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            440..517
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            518..618
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            619..711
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            712..775
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            776..916
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1005
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1043
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1133
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1342
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1480
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1618
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1753
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..2897
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2863..2868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="hexamer: AATACA"
     polyA_site      2897
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="major polyA site"
ORIGIN      
        1 agagtacgcg gcgggcggcg gcggcccgga ggccggtggg gcccggaggc cgcggcgcgg
       61 tcagtcgagc cgagccgagc cgagccgagc cgagccgcgc cgatcgccat cggctccggc
      121 tctcgtgggc ggtcccggcc ggcggcgcgg cctggcgggc cgggcggcgc tgcagcccat
      181 ggagctcgag aacatcgtag cgaacacggt gctactcaag gcccgggaag gtggtggcgg
      241 gaatcgcaaa ggcaagagca agaaatggcg ccagatgctg cagttccccc atatcagcca
      301 gtgtgaggag cttcgactca gccttgagcg tgactaccac agcctatgtg agcgccagcc
      361 cattgggcgc ctgttatttc gtgagttctg tgctacgaga cctgagctga cccggtgtac
      421 tgccttcctg gatggggtgt ctgaatatga ggtgacccct gatgagaagc ggaaagcatg
      481 tgggcgccga ctaatgcaga actttctgag ccacacgggt cctgacctca tccctgaagt
      541 tccacggcag ctggtgagta actgtgccca gcggctagag cagggaccct gcaaagacct
      601 cttccaggag ctgacccggc tgacccacga gtacctgagc acggcccctt ttgccgacta
      661 cctcgacagc atctacttca accgttttct gcagtggaag tggctggaaa ggcaaccagt
      721 gaccaaaaac accttcaggc agtaccgagt cctgggcaaa ggtggctttg gggaggtatg
      781 tgcctgccag gtgcgggcaa caggcaagat gtacgcatgc aagaaactgg aaaagaagcg
      841 gataaagaag cgaaaggggg aggccatggc tctcaacgag aaacagatct tggagaaagt
      901 gaacagtagg tttgtagctg gctttcctga agcacgtgct gtcttctatg ctgctgagat
      961 ctgctgtggc ctggaagacc tgcaccggga acgcattgtg tacagggatc taaagccaga
     1021 gaatatcctt ctggatgacc atggccacat tcggatctcg gacctgggac tggccgtgca
     1081 tgtgcctgag ggccagacca tcaaaggccg tgtgggcact gtgggctaca tggctccaga
     1141 ggtggtgagg aatgagcgct acacgttcag tcctgactgg tgggcgctag gctgcctcct
     1201 gtacgagatg atcgcaggtc agtcgccctt ccagcagagg aagaagaaga taaagcgcga
     1261 agaggtggag cggctggtca aggaagtggc cgaggagtac acagaccgct tttcctcaca
     1321 ggcgcgctca ctctgttctc agcttcttag caaggaccct gctgagcgcc tggggtgtcg
     1381 tggaggtggc gcccgtgagg taaaggagca cccccttttc aagaaactga atttcaagcg
     1441 gctgggagct ggcatgctag agccaccttt taagcctgat ccccaggcta tttattgcaa
     1501 ggatgtcctg gacattgaac agttctctac agttaaaggt gtggatctgg agcccacaga
     1561 ccaagacttc taccagaagt ttgccacagg cagtgtgtcc atcccctggc agaatgagat
     1621 ggtggagacc gagtgcttcc aggaactcaa tgtctttggg ctggatgggt ctgttccccc
     1681 agacctggac tggaagggcc agcccactgc accccccaag aagggattgc tacagagact
     1741 cttcagtcgc caagattgct gtgggaactg cagcgacagt gaggaagagc tccccacccg
     1801 cctctagccc ccaggccgag gcccccaccg gcggttggcg gtagcagcta ctctcagtga
     1861 cgtttacagt tttgcacagt ggtgttcccc attttccacg caagccgtgg attgtggaac
     1921 acagccggaa ctgtccccag tgtcctccgt tcctcagcca ctggcctagc tgagtttgac
     1981 aaggcctggg ccatcttggg acaaaggtgc gtcccttcag ctctctgtgg agctcggggc
     2041 tttatgtatt tatgtatttg tatgaatgta tatagctact agagcgttct taattccacc
     2101 ctggacctgg acaccccagg gcaattagcc ctggccagga aagccaggag ctggcaagag
     2161 gagccagtgc caaactccag gcttctaggg cccagtggag atgagcaagc agagagtggg
     2221 ccaggtcaga cctctgccca cagcactgca ctggccacca ccaacctcca agtgagactt
     2281 gtgcctgcct gttgtggact gaggccacca ccttctggtt gcctaatgct ttccctcttt
     2341 ctcagcattt gtcagggttg gagctggggt ctttgtatcc tctaggactg ggacatagtt
     2401 taaccccaga cttagctgag tgggacccat caaaccactg cccgagatgg gtcgctttct
     2461 gccttccagc tcccagagca cagtagtcct tattctgggt catatcctga agacaccttt
     2521 ttcagaaatg ccccagtcca ggccatagga ggagttagaa ttagccctcc aacattccag
     2581 aatccctcat aaaagtagac acatgcccag caataatcca cccttccctg tgtgtgcctg
     2641 gtgggggtgg gggtgagtgt atatcattgt gtgaaaagag tagggtaagg ctgtgcctaa
     2701 gcccccggac cctagccctg gcctgtccca gactgtgatg gctgtcctga tgctagttag
     2761 gacagcatgg gaccataagg ccctggcttt tccatattta aagccaatgt aagctgccat
     2821 gtgtctctat gtgaccaatg tgagctgcca cgtgtctgtg cgaatacatt ctgagcacac
     2881 ggctggccag ccaccacaaa aaaaaaaaaa aa
//
