LOCUS       NM_001112711            3014 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus G protein-coupled receptor kinase 6 (Grk6), transcript
            variant 3, mRNA.
ACCESSION   NM_001112711
VERSION     NM_001112711.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Glaser,K.M., Tarrant,T.K. and Lammermann,T.
  TITLE     Combinatorial depletions of G-protein coupled receptor kinases in
            immune cells identify pleiotropic and cell type-specific functions
  JOURNAL   Front Immunol 13, 1039803 (2022)
   PUBMED   36451830
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Varney,M.J., Steyaert,W., Coucke,P.J., Delanghe,J.R., Uehling,D.E.,
            Joseph,B., Marcellus,R., Al-Awar,R. and Benovic,J.L.
  TITLE     G protein-coupled receptor kinase 6 (GRK6) regulates insulin
            processing and secretion via effects on proinsulin conversion to
            insulin
  JOURNAL   J Biol Chem 298 (10), 102421 (2022)
   PUBMED   36030052
  REMARK    GeneRIF: G protein-coupled receptor kinase 6 (GRK6) regulates
            insulin processing and secretion via effects on proinsulin
            conversion to insulin.
REFERENCE   3  (bases 1 to 3014)
  AUTHORS   Chaudhary,P.K., Kim,S., Jee,Y., Lee,S.H., Park,K.M. and Kim,S.
  TITLE     Role of GRK6 in the Regulation of Platelet Activation through
            Selective G Protein-Coupled Receptor (GPCR) Desensitization
  JOURNAL   Int J Mol Sci 21 (11), 3932 (2020)
   PUBMED   32486261
  REMARK    GeneRIF: Role of GRK6 in the Regulation of Platelet Activation
            through Selective G Protein-Coupled Receptor (GPCR)
            Desensitization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3014)
  AUTHORS   Wu,W., Sung,C.C., Yu,P., Li,J. and Chung,K.K.K.
  TITLE     S-Nitrosylation of G protein-coupled receptor kinase 6 and Casein
            kinase 2 alpha modulates their kinase activity toward
            alpha-synuclein phosphorylation in an animal model of Parkinson's
            disease
  JOURNAL   PLoS One 15 (4), e0232019 (2020)
   PUBMED   32343709
  REMARK    Erratum:[PLoS One. 2020 Jun 18;15(6):e0235296. doi:
            10.1371/journal.pone.0235296.. PMID: 32555609]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3014)
  AUTHORS   Serafin,D.S., Allyn,B., Sassano,M.F., Timoshchenko,R.G., Mattox,D.,
            Brozowski,J.M., Siderovski,D.P., Truong,Y.K., Esserman,D.,
            Tarrant,T.K. and Billard,M.J.
  TITLE     Chemerin-activated functions of CMKLR1 are regulated by G
            protein-coupled receptor kinase 6 (GRK6) and beta-arrestin 2 in
            inflammatory macrophages
  JOURNAL   Mol Immunol 106, 12-21 (2019)
   PUBMED   30576947
  REMARK    GeneRIF: findings show that chemerin-activated CMKLR1 regulation in
            inflammatory macrophages is largely GRK6 and beta-arrestin
            mediated, which may impact innate immunity and have therapeutic
            implications in rheumatic disease.
REFERENCE   6  (bases 1 to 3014)
  AUTHORS   Fong,A.M., Premont,R.T., Richardson,R.M., Yu,Y.R., Lefkowitz,R.J.
            and Patel,D.D.
  TITLE     Defective lymphocyte chemotaxis in beta-arrestin2- and
            GRK6-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 99 (11), 7478-7483 (2002)
   PUBMED   12032308
  REMARK    GeneRIF: deficiency causes defective lymphocyte chemotaxis
REFERENCE   7  (bases 1 to 3014)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   8  (bases 1 to 3014)
  AUTHORS   Premont,R.T., Macrae,A.D., Aparicio,S.A., Kendall,H.E., Welch,J.E.
            and Lefkowitz,R.J.
  TITLE     The GRK4 subfamily of G protein-coupled receptor kinases.
            Alternative splicing, gene organization, and sequence conservation
  JOURNAL   J Biol Chem 274 (41), 29381-29389 (1999)
   PUBMED   10506199
REFERENCE   9  (bases 1 to 3014)
  AUTHORS   Moepps,B., Vatter,P., Frodl,R., Waechter,F., Dixkens,C.,
            Hameister,H. and Gierschik,P.
  TITLE     Alternative splicing produces transcripts encoding four variants of
            mouse G-protein-coupled receptor kinase 6
  JOURNAL   Genomics 60 (2), 199-209 (1999)
   PUBMED   10486211
REFERENCE   10 (bases 1 to 3014)
  AUTHORS   Hall,R.A., Spurney,R.F., Premont,R.T., Rahman,N., Blitzer,J.T.,
            Pitcher,J.A. and Lefkowitz,R.J.
  TITLE     G protein-coupled receptor kinase 6A phosphorylates the Na(+)/H(+)
            exchanger regulatory factor via a PDZ domain-mediated interaction
  JOURNAL   J Biol Chem 274 (34), 24328-24334 (1999)
   PUBMED   10446210
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009762.8, Y15799.1, AK051405.1 and AW048460.1.
            
            On Oct 29, 2013 this sequence version replaced NM_001112711.1.
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the 3' coding region, which results in a frameshift, compared to
            variant 1. The encoded isoform (c) has a shorter and distinct
            C-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF040747.1, SRR7652917.910421.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               CT009762.8         87287-87459         c
            174-1979            Y15799.1           1-1806
            1980-2994           AK051405.1         1964-2978
            2995-3014           AW048460.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.06 cM"
     gene            1..3014
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="G protein-coupled receptor kinase 6"
                     /db_xref="GeneID:26385"
                     /db_xref="MGI:MGI:1347078"
     exon            1..230
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1909
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /EC_number="2.7.11.16"
                     /note="isoform c is encoded by transcript variant 3; g
                     protein-coupled receptor kinase GRK6"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 6 isoform c"
                     /protein_id="NP_001106182.1"
                     /db_xref="CCDS:CCDS88457.1"
                     /db_xref="GeneID:26385"
                     /db_xref="MGI:MGI:1347078"
                     /translation="MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEE
                     LRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCTAFLDGVSEYEVTPDEKRKACG
                     RRLMQNFLSHTGPDLIPEVPRQLVSNCAQRLEQGPCKDLFQELTRLTHEYLSTAPFAD
                     YLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLE
                     KKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIY
                     HMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAV
                     HVPEGQTIKGRVGTVGYMAPEVVRNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKI
                     KREEVERLVKEVAEEYTDRFSSQARSLCSQLLSKDPAERLGCRGGGAREVKEHPLFKK
                     LNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVDLEPTDQDFYQKFATGSVS
                     IPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPTAPPKKGLLQRLFSRQDCCGNCS
                     DSEEELPTRL"
     exon            231..326
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            327..439
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            440..517
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            518..618
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            619..711
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            712..775
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            776..916
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1107
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1145
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1235
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1444
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1582
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1720
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1855
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..2999
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2965..2970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="hexamer: AATACA"
     polyA_site      2999
                     /gene="Grk6"
                     /gene_synonym="Gprk6"
                     /note="major polyA site"
ORIGIN      
        1 agagtacgcg gcgggcggcg gcggcccgga ggccggtggg gcccggaggc cgcggcgcgg
       61 tcagtcgagc cgagccgagc cgagccgagc cgagccgcgc cgatcgccat cggctccggc
      121 tctcgtgggc ggtcccggcc ggcggcgcgg cctggcgggc cgggcggcgc tgcagcccat
      181 ggagctcgag aacatcgtag cgaacacggt gctactcaag gcccgggaag gtggtggcgg
      241 gaatcgcaaa ggcaagagca agaaatggcg ccagatgctg cagttccccc atatcagcca
      301 gtgtgaggag cttcgactca gccttgagcg tgactaccac agcctatgtg agcgccagcc
      361 cattgggcgc ctgttatttc gtgagttctg tgctacgaga cctgagctga cccggtgtac
      421 tgccttcctg gatggggtgt ctgaatatga ggtgacccct gatgagaagc ggaaagcatg
      481 tgggcgccga ctaatgcaga actttctgag ccacacgggt cctgacctca tccctgaagt
      541 tccacggcag ctggtgagta actgtgccca gcggctagag cagggaccct gcaaagacct
      601 cttccaggag ctgacccggc tgacccacga gtacctgagc acggcccctt ttgccgacta
      661 cctcgacagc atctacttca accgttttct gcagtggaag tggctggaaa ggcaaccagt
      721 gaccaaaaac accttcaggc agtaccgagt cctgggcaaa ggtggctttg gggaggtatg
      781 tgcctgccag gtgcgggcaa caggcaagat gtacgcatgc aagaaactgg aaaagaagcg
      841 gataaagaag cgaaaggggg aggccatggc tctcaacgag aaacagatct tggagaaagt
      901 gaacagtagg tttgtagtga gcttagccta cgcctatgag accaaggatg cactgtgcct
      961 ggtgctgaca ttgatgaatg gaggtgacct aaagttccac atctaccaca tgggccaggc
     1021 tggctttcct gaagcacgtg ctgtcttcta tgctgctgag atctgctgtg gcctggaaga
     1081 cctgcaccgg gaacgcattg tgtacaggga tctaaagcca gagaatatcc ttctggatga
     1141 ccatggccac attcggatct cggacctggg actggccgtg catgtgcctg agggccagac
     1201 catcaaaggc cgtgtgggca ctgtgggcta catggctcca gaggtggtga ggaatgagcg
     1261 ctacacgttc agtcctgact ggtgggcgct aggctgcctc ctgtacgaga tgatcgcagg
     1321 tcagtcgccc ttccagcaga ggaagaagaa gataaagcgc gaagaggtgg agcggctggt
     1381 caaggaagtg gccgaggagt acacagaccg cttttcctca caggcgcgct cactctgttc
     1441 tcagcttctt agcaaggacc ctgctgagcg cctggggtgt cgtggaggtg gcgcccgtga
     1501 ggtaaaggag cacccccttt tcaagaaact gaatttcaag cggctgggag ctggcatgct
     1561 agagccacct tttaagcctg atccccaggc tatttattgc aaggatgtcc tggacattga
     1621 acagttctct acagttaaag gtgtggatct ggagcccaca gaccaagact tctaccagaa
     1681 gtttgccaca ggcagtgtgt ccatcccctg gcagaatgag atggtggaga ccgagtgctt
     1741 ccaggaactc aatgtctttg ggctggatgg gtctgttccc ccagacctgg actggaaggg
     1801 ccagcccact gcacccccca agaagggatt gctacagaga ctcttcagtc gccaagattg
     1861 ctgtgggaac tgcagcgaca gtgaggaaga gctccccacc cgcctctagc ccccaggccg
     1921 aggcccccac cggcggttgg cggtagcagc tactctcagt gacgtttaca gttttgcaca
     1981 gtggtgttcc ccattttcca cgcaagccgt ggattgtgga acacagccgg aactgtcccc
     2041 agtgtcctcc gttcctcagc cactggccta gctgagtttg acaaggcctg ggccatcttg
     2101 ggacaaaggt gcgtcccttc agctctctgt ggagctcggg gctttatgta tttatgtatt
     2161 tgtatgaatg tatatagcta ctagagcgtt cttaattcca ccctggacct ggacacccca
     2221 gggcaattag ccctggccag gaaagccagg agctggcaag aggagccagt gccaaactcc
     2281 aggcttctag ggcccagtgg agatgagcaa gcagagagtg ggccaggtca gacctctgcc
     2341 cacagcactg cactggccac caccaacctc caagtgagac ttgtgcctgc ctgttgtgga
     2401 ctgaggccac caccttctgg ttgcctaatg ctttccctct ttctcagcat ttgtcagggt
     2461 tggagctggg gtctttgtat cctctaggac tgggacatag tttaacccca gacttagctg
     2521 agtgggaccc atcaaaccac tgcccgagat gggtcgcttt ctgccttcca gctcccagag
     2581 cacagtagtc cttattctgg gtcatatcct gaagacacct ttttcagaaa tgccccagtc
     2641 caggccatag gaggagttag aattagccct ccaacattcc agaatccctc ataaaagtag
     2701 acacatgccc agcaataatc cacccttccc tgtgtgtgcc tggtgggggt gggggtgagt
     2761 gtatatcatt gtgtgaaaag agtagggtaa ggctgtgcct aagcccccgg accctagccc
     2821 tggcctgtcc cagactgtga tggctgtcct gatgctagtt aggacagcat gggaccataa
     2881 ggccctggct tttccatatt taaagccaat gtaagctgcc atgtgtctct atgtgaccaa
     2941 tgtgagctgc cacgtgtctg tgcgaataca ttctgagcac acggctggcc agccaccaca
     3001 aaaaaaaaaa aaaa
//
