LOCUS       NM_008170              15521 bp    mRNA    linear   ROD 19-MAR-2025
DEFINITION  Mus musculus glutamate receptor, ionotropic, NMDA2A (epsilon 1)
            (Grin2a), mRNA.
ACCESSION   NM_008170 XM_006521794 XM_358759
VERSION     NM_008170.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Huikang,W., Shiya,C., Di,P., Kiani,F.A., Hao,L., Sha,N., Xuan,L.,
            Abouelfetouh,M.M., Ahmed,Z., Mingxing,D. and Yi,D.
  TITLE     Elucidation of Dexmedetomidine-Induced Analgesic Tolerance
            Mechanisms in Neuropathic Pain With Modulation of SGK1, NR2A, and
            NR2B Expression via the Spinal SGK1/NF-kappaB Signalling Pathway
  JOURNAL   J Cell Mol Med 29 (6), e70372 (2025)
   PUBMED   40099662
REFERENCE   2  (bases 1 to 15521)
  AUTHORS   Song,Q.X., Zhang,Y.Y., Li,Y.L., Liu,F., Liu,Y.J., Li,Y.K., Li,C.J.,
            Zhou,C. and Shen,J.F.
  TITLE     The crucial role of NR2A mediating the activation of satellite
            glial cells in the trigeminal ganglion contributes to orofacial
            inflammatory pain during TMJ inflammation
  JOURNAL   Neuropharmacology 261, 110173 (2024)
   PUBMED   39357737
  REMARK    GeneRIF: The crucial role of NR2A mediating the activation of
            satellite glial cells in the trigeminal ganglion contributes to
            orofacial inflammatory pain during TMJ inflammation.
REFERENCE   3  (bases 1 to 15521)
  AUTHORS   Keith,R.E., Wild,G.A., Keith,M.J., Chen,D., Pack,S. and Dumas,T.C.
  TITLE     Individual NMDA receptor GluN2 subunit signaling domains
            differentially regulate the postnatal maturation of hippocampal
            excitatory synaptic transmission and plasticity but not dendritic
            morphology
  JOURNAL   Synapse 78 (4), e22292 (2024)
   PUBMED   38813758
  REMARK    GeneRIF: Individual NMDA receptor GluN2 subunit signaling domains
            differentially regulate the postnatal maturation of hippocampal
            excitatory synaptic transmission and plasticity but not dendritic
            morphology.
REFERENCE   4  (bases 1 to 15521)
  AUTHORS   Deep,S.N., Seelig,S., Paul,S. and Poddar,R.
  TITLE     Homocysteine-induced sustained GluN2A NMDA receptor stimulation
            leads to mitochondrial ROS generation and neurotoxicity
  JOURNAL   J Biol Chem 300 (5), 107253 (2024)
   PUBMED   38569938
  REMARK    GeneRIF: Homocysteine-induced sustained GluN2A NMDA receptor
            stimulation leads to mitochondrial ROS generation and
            neurotoxicity.
REFERENCE   5  (bases 1 to 15521)
  AUTHORS   Lu,Y., Mu,L., Elstrott,J., Fu,C., Sun,C., Su,T., Ma,X., Yan,J.,
            Jiang,H., Hanson,J.E., Geng,Y. and Chen,Y.
  TITLE     Differential depletion of GluN2A induces heterogeneous
            schizophrenia-related phenotypes in mice
  JOURNAL   EBioMedicine 102, 105045 (2024)
   PUBMED   38471394
  REMARK    GeneRIF: Differential depletion of GluN2A induces heterogeneous
            schizophrenia-related phenotypes in mice.
REFERENCE   6  (bases 1 to 15521)
  AUTHORS   Watanabe,M., Mishina,M. and Inoue,Y.
  TITLE     Differential distributions of the NMDA receptor channel subunit
            mRNAs in the mouse retina
  JOURNAL   Brain Res 634 (2), 328-332 (1994)
   PUBMED   7510577
REFERENCE   7  (bases 1 to 15521)
  AUTHORS   Watanabe,M., Inoue,Y., Sakimura,K. and Mishina,M.
  TITLE     Developmental changes in distribution of NMDA receptor channel
            subunit mRNAs
  JOURNAL   Neuroreport 3 (12), 1138-1140 (1992)
   PUBMED   1493227
REFERENCE   8  (bases 1 to 15521)
  AUTHORS   Ikeda,K., Nagasawa,M., Mori,H., Araki,K., Sakimura,K., Watanabe,M.,
            Inoue,Y. and Mishina,M.
  TITLE     Cloning and expression of the epsilon 4 subunit of the NMDA
            receptor channel
  JOURNAL   FEBS Lett 313 (1), 34-38 (1992)
   PUBMED   1385220
REFERENCE   9  (bases 1 to 15521)
  AUTHORS   Kutsuwada,T., Kashiwabuchi,N., Mori,H., Sakimura,K., Kushiya,E.,
            Araki,K., Meguro,H., Masaki,H., Kumanishi,T., Arakawa,M. et al.
  TITLE     Molecular diversity of the NMDA receptor channel
  JOURNAL   Nature 358 (6381), 36-41 (1992)
   PUBMED   1377365
REFERENCE   10 (bases 1 to 15521)
  AUTHORS   Meguro,H., Mori,H., Araki,K., Kushiya,E., Kutsuwada,T.,
            Yamazaki,M., Kumanishi,T., Arakawa,M., Sakimura,K. and Mishina,M.
  TITLE     Functional characterization of a heteromeric NMDA receptor channel
            expressed from cloned cDNAs
  JOURNAL   Nature 357 (6373), 70-74 (1992)
   PUBMED   1374164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164163.2, AC154190.2, AC121974.3 and AC126022.4.
            
            On Jun 19, 2019 this sequence version replaced NM_008170.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.176123.1,
                                           ERR3363660.655756.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-991               AC164163.2         44977-45967
            992-1182            AC164163.2         46541-46731
            1183-1614           AC164163.2         48239-48670
            1615-2207           AC154190.2         38681-39273
            2208-2322           AC154190.2         92946-93060
            2323-2528           AC121974.3         20036-20241
            2529-2697           AC121974.3         26339-26507
            2698-2851           AC121974.3         26612-26765
            2852-2977           AC121974.3         32147-32272
            2978-3207           AC121974.3         36321-36550
            3208-3368           AC121974.3         45687-45847
            3369-3556           AC121974.3         77189-77376
            3557-3795           AC121974.3         104682-104920
            3796-6395           AC121974.3         110320-112919
            6396-15521          AC126022.4         1-9126
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 5.28 cM"
     gene            1..15521
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /note="glutamate receptor, ionotropic, NMDA2A (epsilon 1)"
                     /db_xref="GeneID:14811"
                     /db_xref="MGI:MGI:95820"
     exon            1..991
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1182
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1614
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             1201..5595
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /note="glutamate [NMDA] receptor subunit epsilon-1;
                     N-methyl D-aspartate receptor subtype 2A; glutamate
                     receptor ionotropic, NMDA 2A"
                     /codon_start=1
                     /product="glutamate receptor ionotropic, NMDA 2A
                     precursor"
                     /protein_id="NP_032196.2"
                     /db_xref="CCDS:CCDS27943.1"
                     /db_xref="GeneID:14811"
                     /db_xref="MGI:MGI:95820"
                     /translation="MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSH
                     DVTERELRNLWGPEQATGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGD
                     DTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLK
                     IMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQ
                     LKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGL
                     ISVSYDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTL
                     HQFMVNVTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPR
                     YKSFSDCEPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGM
                     NVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMA
                     VGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLL
                     IVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKG
                     TTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRF
                     GTVPNGSTERNIRNNYPYMHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAG
                     RDEGCKLVTIGSGYIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTG
                     ICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDR
                     PGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNMNSSR
                     MDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHK
                     DSLSNYVFQGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNP
                     VSQRDEKTAENRTHSLKSPRYLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKR
                     SMASKYPKDCSEVERTYVKTKASSPRDKIYTIDGEKEPSFHLDPPQFIENIVLPENVD
                     FPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKHFTLKDKGSPHSEGS
                     DRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPAT
                     REEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLL
                     EGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLV
                     IGRCPSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKN
                     NMYSTPRVLNSCSNRRVYKKMPSIESDV"
     exon            1615..2207
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2322
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2528
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2697
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2851
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2977
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3207
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3208..3368
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3369..3556
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3557..3795
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..15521
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10177..10182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /note="hexamer: ATTAAA"
     polyA_site      10215
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
     regulatory      15489..15494
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /note="hexamer: AATAAA"
     polyA_site      15521
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /note="major polyA site"
ORIGIN      
        1 ctctgacatc agagtgggtt cccatatcag ggtggtgagg cattgagaga agcatcctgg
       61 gcgggtgtgt gcgtgtcggc gtgccttgcg tggaacagag attgctgcac ccagcgccag
      121 gcactggagg aggaggaatc cgtagcagag atctgaataa ttgattcttc agcagcacca
      181 gacgcccggg agcgggcgga gagcgtggtt tcagcaccaa ggttgcgcgc caccgcaagc
      241 tccccccgtc accagccaga cacctaggtc ggccttgttc cgctgccgcc gtgcgctcca
      301 gaagagggtg gccgcgctcc gcgtcgccgc ctagggactg cggggtctgc cttggcccgc
      361 ctccggggtg ggcggctgca ggcggaccag agctccaaac aacgtcggat tgcgcggggg
      421 ttgctgcgtt gcgtctcgct ggctgagaac aagtcctaag ttgctgtggt gggcgcctct
      481 gctttcctct atcctcctag gcgaccgcag cagaagcgtg ccctaggatc ctggcttctc
      541 cagtgttgtg aaccgcggtg gccaggctct ttcctgggct cccgctgacc aagtgaggaa
      601 ccgaacgccc agctcggagc tggcccttca gcgaaagggc caggagggag ctgcggtcct
      661 ctgcctgcgc ccttctccgc cagggaccgc ggggatcccc ggccacacgc gcgcgcgcgc
      721 tcccacactc actcacacac acacactcac acactcacgc acgccaagca ggagcaggac
      781 tgcggagagg gtgtccaggc gcgctcagag gacctggcag ccgctgtccg gagtggagca
      841 taaagctgag ccagggctct aggagagagg ctcctggggt gctgtgcctg agcatggggc
      901 cggatgagct ctgagagtcg cggcggcagc aatcagccct ggagatgacc aggggtggcc
      961 actgctgaga actatgtgga gagaggctgc gagccctgct gcagagcctc cggctgggat
     1021 agccgccccc cgtgggggtg atgcggacag cgcgggacag ccaggggagc acgcaggggc
     1081 cgcagcatgc gggaacccgc taaacctggt ggctgctgag gcggccgaga tgctcgtgcg
     1141 cgcagcacgc cccattgcat cctccacctt ctccggctac agggacccta agtggcgacc
     1201 atgggcagac tgggctactg gaccttgctg gtattgccgg cccttctggt ctggcacggt
     1261 ccggcgcaga acgcggcggc ggagaagggt actccagcgc tgaacattgc ggtgctgctg
     1321 ggtcacagcc acgacgtgac agaacgcgaa cttcgaaatc tgtggggccc ggagcaggca
     1381 accggcttgc ccctggatgt gaacgtggtg gcgttattga tgaaccgcac tgaccctaag
     1441 agcctcatca cgcatgtgtg cgacctcatg tccggggcgc gcatccatgg cttggtgttt
     1501 ggagatgata cggaccaaga ggctgtggct cagatgctgg attttatctc ctcacagact
     1561 ttcatcccca tcttgggcat tcatgggggt gcatctatga tcatggctga caaggatccg
     1621 acatccacgt tcttccagtt tggagcttcc atccagcagc aagccacggt catgctgaag
     1681 atcatgcagg actatgactg gcatgtcttc tccttggtca ccaccatctt ccctggctac
     1741 agagacttca tcagcttcat caagacaaca gtggacaaca gctttgtggg ctgggatatg
     1801 cagaacgtga tcacactgga cacctctttt gaggacgcca agacacaggt ccagctgaag
     1861 aagatccact cctctgtcat cctgctctac tgctccaagg atgaggctgt cctcatcctg
     1921 agcgaggctc gctctcttgg cctcaccggc tacgatttct tctggattgt ccccagtttg
     1981 gtctccggga acacagagct catccccaaa gagtttccat cgggtctcat ttcagtctct
     2041 tacgacgact gggactacag tctggaggca agagtgagag acggtcttgg gatcttaacc
     2101 actgccgcat cttccatgtt ggagaaattc tcctacattc ccgaggccaa ggccagctgc
     2161 tacgggcaga cagagaagcc ggagaccccg ctacacactc tgcaccaatt tatggtcaat
     2221 gtgacttggg atggcaagga cttgtccttc actgaggaag gctatcaggt gcaccccagg
     2281 cttgtggtga tcgtgctgaa taaggaccgg gaatgggaaa aggtgggcaa gtgggagaat
     2341 cagactctga gcctgcggca tgctgtgtgg ccaaggtata agtccttttc tgactgtgag
     2401 ccagatgaca accacctcag cattgtcacc ttggaggaag cccccttcgt catcgtagaa
     2461 gacatagacc cactgactga gacctgcgtc aggaacacgg taccctgtcg gaagtttgtc
     2521 aagatcaaca attcaaccaa cgaagggatg aatgtgaaga agtgctgcaa ggggttctgc
     2581 atcgacatcc ttaagaaact gtccagaact gtaaagttca cctatgacct ctacctggtg
     2641 accaatggga agcatgggaa aaaggttaac aatgtgtgga atggaatgat aggcgaagtg
     2701 gtctatcaac gagcagttat ggccgtgggc tccctcacca tcaatgagga gcgttcagaa
     2761 gtggtggact tctccgtgcc ctttgtggag acaggaatca gtgtcatggt ctccaggagt
     2821 aatggcactg tttccccttc tgctttcctt gaacccttca gcgcctctgt ctgggtgatg
     2881 atgttcgtga tgctgctcat tgtctctgcc attgctgtct tcgtttttga atacttcagt
     2941 cctgttggat acaacagaaa cttagccaaa gggaaagctc cccatgggcc ttcttttacc
     3001 attggaaaag ctatatggct cctctggggc ctggtcttca acaattctgt gcccgtccag
     3061 aatcctaaag gcacaaccag caagataatg gtatcagtgt gggccttctt tgccgtcatc
     3121 ttccttgcaa gttacacagc caacctggct gccttcatga tccaggagga gtttgtggac
     3181 caagtgactg gcctcagtga caagaagttc cagagacctc atgactattc tccgcctttc
     3241 cgatttggga cagtacccaa tggaagtaca gaaaggaata ttcgtaacaa ctacccctat
     3301 atgcaccagt acatgaccaa attcaaccag aggggcgtag aggatgcctt ggtcagcttg
     3361 aaaactggga agttggacgc tttcatctat gacgcagctg tcttgaacta caaggccggg
     3421 agggatgaag gctgtaaact ggtgaccatt gggagcgggt acatctttgc caccacaggc
     3481 tatggaattg ctctgcagaa gggctcaccc tggaagaggc agattgacct cgctctgctc
     3541 cagtttgttg gtgacggtga gatggaagag ctggagacac tgtggcttac gggcatctgc
     3601 cacaacgaga agaatgaggt gatgagcagc cagctggaca tcgacaacat ggcaggagtt
     3661 ttctacatgc tggctgcagc tatggccctc agcctcatca ccttcatctg ggagcacctc
     3721 ttctactgga agctgcgctt ctgcttcaca ggcgtgtgtt ctgaccggcc cgggctgctc
     3781 ttctccatca gcaggggcat ctacagttgc atccatggag tgcacattga agaaaagaag
     3841 aagtctccag acttcaatct gactgggtca cagagcaaca tgctaaagct tctccgctca
     3901 gctaaaaaca tctccaacat gtccaacatg aactcctcgc gaatggactc acccaaaaga
     3961 gctgctgact tcatccaaag aggctcactt attgtggaca tggtttcaga caagggaaat
     4021 ttgatatact cagataacag gtcctttcaa gggaaggaca gtatatttgg agaaaacatg
     4081 aatgaactgc aaacatttgt ggccaacagg cacaaggata gtctcagtaa ctatgtgttt
     4141 cagggacagc atcctctcac tctcaatgag tccaacccca acacagtgga ggtggctgtc
     4201 agcactgaat ccaaagggaa ctcccgaccc cggcagcttt ggaagaaatc catggagtct
     4261 ctacgccagg attctctaaa ccagaaccca gtctcccaga gggatgagaa gactgcagag
     4321 aataggaccc actccctaaa gagtcctagg tatcttccag aagaggtagc ccattctgac
     4381 atttctgaaa cctcaagccg ggccacatgc cacagggagc cagataataa taagaaccac
     4441 aagaccaagg ataacttcaa aaggtcaatg gcctctaaat accccaagga ctgtagtgag
     4501 gttgaacgta cctacgtgaa aaccaaagca agttctccca gggataagat ctacaccatc
     4561 gatggtgaga aggagcccag cttccactta gatcctccac agttcattga aaacatagtc
     4621 ttgcctgaga atgtggactt cccagatacc taccaagatc acaatgagaa tttccgcaag
     4681 ggggactcca cactgcccat gaacaggaac ccactacaca atgaagatgg gcttcccaac
     4741 aatgaccagt ataaactcta tgccaagcac tttaccttga aagacaaggg ttccccacat
     4801 agtgagggca gtgatcgata tcggcagaac tccacgcatt gcagaagctg cctctcaaac
     4861 ctgcccacct actcaggcca ctttaccatg agatctcctt tcaagtgtga tgcctgtctg
     4921 cggatgggga acctctatga cattgatgaa gaccagatgc ttcaggagac aggcaaccca
     4981 gctactcgtg aggaggccta ccagcaggac tggtcacaga acaacgccct ccagttccag
     5041 aagaacaagc taaagattaa tcgacagcac tcctatgata acattctcga caaacccagg
     5101 gagatagacc ttagcaggcc ctctcgtagc ataagcctca aggacaggga aaggctactg
     5161 gagggcaact tatacgggag cctgttcagt gtcccctcaa gcaaactctt ggggaacaaa
     5221 agctcccttt tcccccaagg tctggaggac agcaagagga gcaaatctct cttgccagac
     5281 catacctctg ataatccttt cctccacacg tacggggatg accaacgctt agttattggg
     5341 agatgtccct cggaccctta caaacactca ttgccatcac aggcagtaaa tgacagctat
     5401 cttcggtcat ccttgaggtc aacagcatca tattgctcca gggacagtcg gggccacagt
     5461 gatgtgtata tttcagagca tgttatgcct tatgctgcaa ataagaataa catgtactct
     5521 acccccaggg ttttaaattc ctgcagcaat agacgtgtgt acaagaaaat gcctagtatt
     5581 gaatctgatg tctaagatct tccatcagtg tttatctata aggaaacatg tagaatggcc
     5641 aacattatag agggtaaacg ttggatgtcc aatagcaccc tactaggagg aagaggatat
     5701 agggaggtat ttttttgttg gctcttttgc acatggctcc atgccataat cttccactca
     5761 aggaatctta tgaggtatgt gctgagcaca gcacatacca gataggtgaa tccttaacca
     5821 aaaacaaatg aatacatgtg ggcaggtctc ccagacatgg tgactgggta tggcggcaat
     5881 aatggtgcat cagacggtga tggttatatt gtggttgcct atattccaaa ttccatgaag
     5941 atcaatccac catgtaactt ccccatcaga aatgcctcac agtttctcta atacagaata
     6001 agcaatatgg tatgcatgta agtctgaccc agacaataag acagttaaga atgcatctgc
     6061 actgtaataa gattgacatg tgcaaggttt aagaagtttg gcttgtaaca gttctcagct
     6121 ttcttgttat gccttccatt acaaccctgg atcagccccc acaaactcca ggttcccccc
     6181 caaagaatag caaatcagtg tgtttgtggt acctacacta ctttcattgt agtcttcccc
     6241 caagacacac ctggaacaaa tagccacagg gactctcagg tggagaactg taagaatctc
     6301 tttggatgtt aatgtgtgtg tatctctcac cctcagcttc agtggtcttg ttcggagctg
     6361 cataaacact tttatgtctt cgatatctaa gtgtggatct tctgtctatg acatagtggc
     6421 cgttgtagtt tatcttgaag actcttatgt acgtaagttt gcattcctcc cttctggtga
     6481 tgactcaggg ttgtatagta tctcttaccc gatccctccc agagtaattg taacatgttc
     6541 tgtccccaga cagccacgat ggtgtctaat tagctatgtg ttgtgttccc agagtcgtta
     6601 atgtggtgac atgtgccagc agccatgtgt aatctagaag aagtacaatg ccatctgtgt
     6661 gccccaggct agcatggttt tacatttatg tcccttaatg tcaggaaact ctattagaca
     6721 cttatttcta tacaaatcac ctcaaatcaa aagctttcag acatcagcca cttgtgcatc
     6781 tccccacccc caaccccagc accctcccaa ctatggataa actcagtttt aaaaaggggg
     6841 gatttctttc ctctgaaatg tgcaccttct tggaggtgca cctcttgccc agcaccaaca
     6901 gaaaccaatg agaagtcttc aaagcatttg tcattcctga gctgcctgcg ttgtcctctt
     6961 cttggcccac taagcctgcg catatatgat agaacagata gtcattagtt taaaaaaatg
     7021 aagccagtga ggggaatatt cgtgcatgga aaatttctta gcacataatt ttttaccaca
     7081 tttttaaaaa gactcaaaat gtagttatga tactttatta tacaaggaca taccacagaa
     7141 acagaaaata atatgaatta aacattttat atggttcttt tctagcattt agtatatttc
     7201 aaacgcacta ttgtccttga aatagcctgg ctagctggcc atggtatttt gcatgagact
     7261 ttagtcagtt gggtaactct ctcccatgaa gaattctaac catttcttgt gttttctctt
     7321 actgaatcct aggttgggtt cttggagaaa gggacagcgt gacaggttga aatttcatca
     7381 catttctttt cctcatgctg taatgttttg taggtggaat tatcctgtag ttgagcaggg
     7441 aagggtctct tagatcttgg ggctgcttca gacgaagcta gcagtggaaa agttcatttg
     7501 acctacagtg ttctacaatg acaaaaagtg aggcaataga ctagagggcc agtggtggag
     7561 tggcataggg gtgaagcatc tctcttcctg tgctccttca gaacacagag ggggctaggg
     7621 tggagctaga gggcttgggt ggaacttgag gttgataagc tttaaagtca cattttatcc
     7681 cttggatgga agcatctttt ccttccccaa gtcccagaga tggagaaatg tgcgttagag
     7741 aacctggaat agtctcccca gttttttacc ttaaaactgg gcttcagtgt ttgtagtgtc
     7801 ttttaagccc ttcactttgt ttctctattg catcccaaac tgtatttctg tattcctcct
     7861 gctagggcca ctgctgtgtc atcacagaac tgactcttaa gcctccctgg aatacagaat
     7921 ggtgacttgt cacaaatcct cgaagcattc tgcttttcta gattgtcctc acagcccagg
     7981 gtgctggtga atgcccacat ttcagtggga gtacagatga tcctcctact gcctcctcaa
     8041 acccttctct ggaaaattca gagatcattt ctgctattac ttctatacaa aagaagttaa
     8101 aatgaggctt ttggaagaat cagccacata atgggaaaga atgcatttgt tataacacca
     8161 agatatttac aaaaataagt cttggtcatt aggggctgta tgataaacat ttgcttttcc
     8221 ccctccacta atccactgac tcttataatt gatgatttca aagaagaaaa cgaggtggtc
     8281 aggttccaag tgaaaggtaa ccatgtgggc ttgtttgaag gagtcctctc aaaaactaat
     8341 ccagggagtg gcaaatggca cctaaggtgg tgatgaggac tggacaggag gccctggcct
     8401 gagcatcagg tagaggaccc ctgtgttaga cccaacctct gagttcttag actcacagat
     8461 gctaagaagc cagtgtcttt gaatccaagg cctgaccttt ccccggacta agtaagggca
     8521 gctgctcttg gcagttgtct tggcagctct tgtgctttcc actttggaaa ggagcagcgt
     8581 ggtcctcaga tgggaatacg tggaggcaaa tagccatgct tctgtcctta cacccgaact
     8641 gatttacata gtctgcataa gcgaagctcc ccgagcgagc atgaccctca ctctgccaag
     8701 cctcagtcct ctgggaaacg ggtccaccat cctaacacct gtaggtttta atatacttct
     8761 ggcttcaatg ctgcaagaag gcaagcccct atggctctgt cttcctgcat gtgactcaag
     8821 ggatgatgac ctcagcactg gtgacaattt cctgccatgc tctgggttct tctttccctg
     8881 cctcaaagga ttcttgagaa gagttaatac ttagctttct ggaggaagca tcaaaatcta
     8941 tccatttcag caaaaactgt caaaccaatt ctcacttagg ttctgggcag aaaaaaagaa
     9001 aattacacct tgtttacaaa tgaaatagtt tcatttaaat gggactttag gcaaggcagt
     9061 taattagtca aaagatccat ggtagtcaca agtctctcgg tgtttattcc atttggtatg
     9121 tctactgctg ttatgtattt aatattctgc atccttctca ggaaatgaca aatgccctga
     9181 attctttgca aatagaattg ctaatgaaaa agaaagttgg tgttaattaa atgatgatta
     9241 aaaatttatt tcccttaaac ctaaaaagta aagctttaat aattgcttgt catataaata
     9301 aataccttgc actgaggttt cttgtggttt aagacctaaa tagatactga ttcaagaaac
     9361 caaaatctct ttcaaaggag ttgaaggcac ccaatagcat tatgcgcatt cctggactgt
     9421 ttctttgtgt ggagctgtct tgtgtgaatc cttcagcaaa ggacagaagc tgtttcaagg
     9481 tggagctgtt tggcactaac gttggctata atagacttac ctggacttca agaaataaaa
     9541 tttaaacagg aggaagccaa atttatcggt ccatatttaa tagtaattca agctctaaag
     9601 gaaagagccc tagggctgga gagatggctc agtggctgct cttccagagg tcctgagttc
     9661 aattccctgc aaccacatgg tggctcacaa ctgtctgtaa ctttagttcc aggggatctg
     9721 gcacctcaca cagacacata tgcaggcaaa gcatcaatgc acacaaaaat aataaatctt
     9781 taaaaaacga aagaagaaag aaagacagaa aagagacctt ccttccaaaa gcattgttca
     9841 gttgcacttg agtcaagaat tggcctgtct gtgcctccct cttcctttgg ttaaataggg
     9901 tcaaaatgtt cattgagagt tttggggtct ctacttgaaa agtttttcca atgcagagtt
     9961 gccctttttc agtcagccat tttcctatgt actgtgttca tctctaattg tctctgatga
    10021 ggtcagcttg atacagatca gagatgattg aatgcacttc tgtgaataca gttgctcatg
    10081 tacacatgta tatattatac atgcttgatt tctacagctc acacttaagt gtattatcca
    10141 agcaaacctg tacatattgt aaagtttatg ttaaaaatta aatggtggcg gtggtgtgaa
    10201 cacacaggaa aaaaattctg attattttga taggagggta tttatgtaga gaaggcatta
    10261 tattttttta actatcagct ctaggaagga tgatagggct cagggggatt tgagcactga
    10321 tgctctttgt ccccttaagg aacaaaatta tggggagaag agtgtgcaat atatgctgct
    10381 tatgttggct ttggaatatg atactggtgt ggacttatat gtgacttgtg taaacccatc
    10441 aaaagacata gattttctat ttaaatttcg tttctctttt ggctcattct gtgcctagaa
    10501 gtgctatcag catagaaaaa gttcaaggta ccttcttgga agggtcttag attaaatgga
    10561 tttgtggaat gataccaaga ctttaggaaa ggggaataag ggagggtgac aagggacagg
    10621 agtctgtcta agatgaagtc agaaagcatt ggagttgggg ctggagccca agactcagtt
    10681 ctgattccta gcttggtacc ctttactagg ctgacctatg tctgtgtttc ttttaggaaa
    10741 acacccgcca tgcattcctc tggagtaaga gtcagtcaca gaactgacct ggctctcatt
    10801 gcctcctgct ctttgccgat tcaggcatgg ctctttgaat ttggtttttc tttaagctcc
    10861 tttgcgatcc ctcattctct caagatctcg aggaagaggg gatctttttt cagggatcta
    10921 tcctctagat aagatccaca aaggacacag tttaaagctt caaaccaaag attttttaaa
    10981 aagctgtcag ccatcagtga ttatactgtg ctgttggaca tgtgacacca atctgcctgt
    11041 agggaacggg cactgtgaca ctttcctcta atgtccagtt tggcactgag ggcatgcaaa
    11101 tggactggaa agtagaacct ctttctgtaa gcagtccctg gtttgaaaag tggattccaa
    11161 aatacaaaag ataagccaca actgtaaaac aaaaacagat taaatgctga aaattagaat
    11221 cagactgtaa tgctattgta aggagaccaa aaattaattg atactgaaat caaacacctt
    11281 tgaaaagtcc tttcctgagt tttcagggac tttggaaaac gctgttagtg tcactggaaa
    11341 tagtcttgat ttatttaaac cttctgagat ggtgaggcag agcacaggag cagctaaaca
    11401 aagggggaaa cctcatcctt cctttttttt tttttttttt tttttttttt gcatgagtag
    11461 tcttgggtct ggctccaacc aaccctgcat gacttaccaa agcatgcctc aaatgcttca
    11521 aagggttgaa ataatccttc aaaggacaaa ggattggaaa atagactgtg atgtaagaga
    11581 aaaagggaaa aaaagttaag ggaggaagat gaagaggggg ggaggagaag gagggggagg
    11641 aggaggaggg gaaggaggag gtgggggggg aggaggagga ggaggaggag gaggaggagt
    11701 atggaaagct ttcccagttg ggtcatttat aacttgtaaa aatgtttaat acttcagggt
    11761 gtggatacgt caggggaaag agtaggaaat gcactttgta atgtcccaag tgcctatgtg
    11821 tggcaaaaag aagtgtcagg ttcgctagtg aaaacagagg tcaggaatgt acagaagaga
    11881 agggaaaccg gtgtgtgtct ggaggacaga gggatgtaga tggtgtggaa gatgttcaca
    11941 cactgtgtgt gccagtctgt gcatatgaac ttgttgtagc ttctgcttgt cacaagtatg
    12001 caccatgatc agcatctgaa tcttcaaata cccagctatg taaactctga atggagttta
    12061 gaatttaagt atgattgccc ccattttgaa gataagggga ctgaggctaa tgctgttata
    12121 agcctcacca gcaacaagga caacaaggct gataaatact catgtcgcat gtaccctagg
    12181 aatgtctgct cttctccagg gaggtgagac tggaagcaga aagagatttg ctaatttgag
    12241 gtccccagga ctttccctaa gtgtcctatg caggctgcct ggtttggggt atatcaagag
    12301 ctcctgggga ccccttgtct gtcgcagtcc cttcacagac tagagcctgc tcatgcctct
    12361 ccccgcctgg tgcttgcact cagcagtagc ttttcatttc tttttttttc cttttttttt
    12421 ttttttcttt ttgcttagga aacttacctt ctctaattag catgtgccta atcttaaata
    12481 tctctttccc aatctccttt cttctttaac agttggcatc agggctagta ccattaaacg
    12541 tatgtatata gtatactttc acagatgtgt ggacacagtc acacacatac atatgcacac
    12601 actgccttgt ggtcctcaat caaagctctc ttaaacacca tgaccccttt ctaaaaagga
    12661 tgctatttgc aaattcactg gtcctcctgc tcatctgcat tggtttattt ttgccaggca
    12721 gacagaatga ggttcagaga tagggcaggg aatggggagt agttttgcat acatgattga
    12781 gtgctttagt gtcagagtga ttgagggcca gctattaaaa tgctgttgtt gtagttgttg
    12841 tagttgttgt tgtttcattt gtttaattgc cttagagatg cgctcctggt cattagcatc
    12901 aggataattg ttgatgcacc aacatttgtt aactttatcc ctaggaagag tgttgatctg
    12961 agatgcttcc atagcacttt ataggagcag agagtggcat ggtgtctaac atgtcactca
    13021 ggtgatgctc cgaagcacac aagtgtcatc tgtgtatccc ctcccaccca tgttccaaac
    13081 cacacttctt ttctggcatt tatgtttaca gtgaggtggg acacactagg cccattttgt
    13141 agatgcaaag attgagctgg gatcacaagg gaggttgttt tggaattaca ttttcataga
    13201 aaaatccagt cattgttctc aatgcttctg aggagtcttc aaacagtgcc cgcagcttga
    13261 tttctgagaa taattctctt ggtattgtca agctgatggt caaaagcctc ataacataga
    13321 ggttactaga gctagggata cagctcaact agtagagtgt ttgcacagca cacgtgtagt
    13381 ctgggttgca tccccagcat ggcaagcata aaccaggtgt gctagcacat gcctgtgatt
    13441 ctggaactcg ggaagcagag gcaggaggat tatgagttca aggttatgcc caggcaatat
    13501 tgtgagttgg aggcctacaa gagacctagt cttgaaaaac taaaacaaac cacagaggtc
    13561 attattatat aatagctatt ctgagactat gtgtttctgc aagaggcgag gagactccca
    13621 gggatgctga actatgcaga aagtggagtg cctcaatcaa cagggccagg agcttcacct
    13681 ctttggagct gtatttcaaa gcacacaaat ctcctgtgaa ccagaaccaa actattcaca
    13741 atggactcct tcctcagatt aacttcccaa ataaggcatc agcaggcttc aggaagaagc
    13801 aagacagaag caatcacccc tcaccagctc cttttagata tcatatcagc agatcgatga
    13861 gtctgcattt ttacaatgct agatcatttc tcatctcctg tagccctggg tcatggcctc
    13921 ttccacttaa ttctctttca cacctttggg aaggattgaa aatgaacctt caaaaggata
    13981 ttagcataac acctagccaa taccacagat gccttttaaa ttaataaggt taattgttct
    14041 ccagctaaca taagtttgtc tttggcattt tcaatatttc tcattgatct gacatttttc
    14101 tgtttcaagc ttttaaaggg ctccaatcaa agactattga taacagagca aatagcgaag
    14161 gttcagaaat gtactaatac attgtctgtt tttccatgag aaacaattac aaactttgaa
    14221 ttcattgacg gtttttcata ttagccatgt ataatacatt gaattgtttc tcttcccagt
    14281 caggctaatt ctaatgaaca aacagtattg acttttaaaa cctgttttcc tttttaaagt
    14341 atcttaaatg tattaagtct gtagacacag ctctgaggcc gcggccatta gggaagaacc
    14401 ccatgtcaga acagaagaat cagacagggt tttgtggctc atctgagcaa tcatctttgg
    14461 aaatttgatg gatttggaaa gctcaaactg acaactctaa cattcacatt accctgatat
    14521 tttcactggg taggatgtaa atgaataaaa atttttaaag aagatattaa gtcagggtta
    14581 tgtaggaaac atggttgttg taaaacacaa aaagaaatgt ggctcggatg cttcccaagc
    14641 ctgagggtcc ccaaagatgt ttttatggtt gtcttcagag tatgaatgtt agccttgttc
    14701 attcccctga taagacatat taatcggtag aattcatcat agtttatatg gtcctgggac
    14761 atttgtgtat gtgtgcttat tatattttga actgaatatc agacagaatt aggtgagtta
    14821 attggtggct tgaataagaa ataggtcaca aaagtcataa ataatgttct tctcaaacag
    14881 taaaagaagg taaactattc attttctagc catgctttaa gactcaaatt atgtacaagc
    14941 tacccattgc ccacaggaga gttattgaat tagatagctt attcataaag ggtttccata
    15001 tgagtccatc tttaacccag tgtgaatggg attgaactca tgcagagggc ttccatccac
    15061 aactacatta atctgcatct aaaactcacg ttaggactgc cttgacgccg tgtacgatgg
    15121 atccaaggat agctgtgtgc tccatcagtg gtcctcattt gggagtagat attcacctcc
    15181 atgtcacagc agtttatact gtcatgtctg atccgctgca tgaggaagtg ctaatgactg
    15241 tattaactat ttccaagcat agctacaaaa ctacacgttt gcttaacacc tttcgatgat
    15301 agaggaagta tatactatgg ttgtcttctt gtagtatgtc tttttaattt tgttcaatgt
    15361 gatatcaact gttttaagga atgggatttg acatgtgtgt gcaaactgat atgtacataa
    15421 ctcccggggc aacacaagtg cccatcatac agtgtaaaaa atgtaaatag aggaaatttc
    15481 atttttctaa taaagataat ttctgccact tgatatgaaa a
//
