LOCUS       NP_032196               1464 aa            linear   ROD 12-JUN-2025
DEFINITION  glutamate receptor ionotropic, NMDA 2A precursor [Mus musculus].
ACCESSION   NP_032196 XP_006521857 XP_358759
VERSION     NP_032196.2
DBSOURCE    REFSEQ: accession NM_008170.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1464)
  AUTHORS   Barnes,S.A., Thomazeau,A., Finnie,P.S.B., Heinrich,M.J.,
            Heynen,A.J., Komiyama,N.H., Grant,S.G.N., Menniti,F.S.,
            Osterweil,E.K. and Bear,M.F.
  TITLE     Non-ionotropic signaling through the NMDA receptor GluN2B
            carboxy-terminal domain drives dendritic spine plasticity and
            reverses fragile X phenotypes
  JOURNAL   Cell Rep 44 (3), 115311 (2025)
   PUBMED   39983718
REFERENCE   2  (residues 1 to 1464)
  AUTHORS   Topolski,M.A., Gilmore,B.L., Khondaker,R., Michniak,J.A.,
            Studtmann,C., Chen,Y., Wagner,G.N., Pozo-Aranda,A.E., Farris,S. and
            Swanger,S.A.
  TITLE     Input-Specific Localization of NMDA Receptor GluN2 Subunits in
            Thalamocortical Neurons
  JOURNAL   J Neurochem 169 (3), e70049 (2025)
   PUBMED   40123534
REFERENCE   3  (residues 1 to 1464)
  AUTHORS   Huikang,W., Shiya,C., Di,P., Kiani,F.A., Hao,L., Sha,N., Xuan,L.,
            Abouelfetouh,M.M., Ahmed,Z., Mingxing,D. and Yi,D.
  TITLE     Elucidation of Dexmedetomidine-Induced Analgesic Tolerance
            Mechanisms in Neuropathic Pain With Modulation of SGK1, NR2A, and
            NR2B Expression via the Spinal SGK1/NF-kappaB Signalling Pathway
  JOURNAL   J Cell Mol Med 29 (6), e70372 (2025)
   PUBMED   40099662
REFERENCE   4  (residues 1 to 1464)
  AUTHORS   Wang,J., Zhang,J., Li,J., Gao,Q., Chen,J., Jia,C. and Gu,X.
  TITLE     Cortex-Specific Tmem169 Deficiency Induces Defects in Cortical
            Neuron Development and Autism-Like Behaviors in Mice
  JOURNAL   J Neurosci 45 (9), e1072242024 (2025)
   PUBMED   39779369
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1464)
  AUTHORS   Kang,M., Yoon,S.H., Kang,M., Park,S.P., Song,W.S., Kim,J., Lee,S.,
            Park,D.H., Song,J.M., Kim,B., Park,K.H., Joe,E.H., Woo,H.G.,
            Park,S.H., Kaang,B.K., Han,D., Lee,Y.S., Kim,M.H. and Suh,Y.H.
  TITLE     Cd99l2 regulates excitatory synapse development and restrains
            immediate-early gene activation
  JOURNAL   Cell Rep 44 (1), 115155 (2025)
   PUBMED   39808524
REFERENCE   6  (residues 1 to 1464)
  AUTHORS   Watanabe,M., Mishina,M. and Inoue,Y.
  TITLE     Differential distributions of the NMDA receptor channel subunit
            mRNAs in the mouse retina
  JOURNAL   Brain Res 634 (2), 328-332 (1994)
   PUBMED   7510577
REFERENCE   7  (residues 1 to 1464)
  AUTHORS   Watanabe,M., Inoue,Y., Sakimura,K. and Mishina,M.
  TITLE     Developmental changes in distribution of NMDA receptor channel
            subunit mRNAs
  JOURNAL   Neuroreport 3 (12), 1138-1140 (1992)
   PUBMED   1493227
REFERENCE   8  (residues 1 to 1464)
  AUTHORS   Ikeda,K., Nagasawa,M., Mori,H., Araki,K., Sakimura,K., Watanabe,M.,
            Inoue,Y. and Mishina,M.
  TITLE     Cloning and expression of the epsilon 4 subunit of the NMDA
            receptor channel
  JOURNAL   FEBS Lett 313 (1), 34-38 (1992)
   PUBMED   1385220
REFERENCE   9  (residues 1 to 1464)
  AUTHORS   Kutsuwada,T., Kashiwabuchi,N., Mori,H., Sakimura,K., Kushiya,E.,
            Araki,K., Meguro,H., Masaki,H., Kumanishi,T., Arakawa,M. et al.
  TITLE     Molecular diversity of the NMDA receptor channel
  JOURNAL   Nature 358 (6381), 36-41 (1992)
   PUBMED   1377365
REFERENCE   10 (residues 1 to 1464)
  AUTHORS   Meguro,H., Mori,H., Araki,K., Kushiya,E., Kutsuwada,T.,
            Yamazaki,M., Kumanishi,T., Arakawa,M., Sakimura,K. and Mishina,M.
  TITLE     Functional characterization of a heteromeric NMDA receptor channel
            expressed from cloned cDNAs
  JOURNAL   Nature 357 (6373), 70-74 (1992)
   PUBMED   1374164
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164163.2, AC154190.2, AC121974.3 and AC126022.4.
            
            On or before Apr 18, 2018 this sequence version replaced
            XP_006521857.1, XP_358759.2, NP_032196.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.176123.1,
                                           ERR3363660.655756.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 5.28 cM"
     Protein         1..1464
                     /product="glutamate receptor ionotropic, NMDA 2A
                     precursor"
                     /note="glutamate [NMDA] receptor subunit epsilon-1;
                     N-methyl D-aspartate receptor subtype 2A; glutamate
                     receptor ionotropic, NMDA 2A"
                     /calculated_mol_wt=162521
     sig_peptide     1..27
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2919
     Region          32..387
                     /region_name="PBP1_iGluR_NMDA_NR2"
                     /note="N-terminal leucine-isoleucine-valine-binding
                     protein (LIVBP)-like domain of the NR2 subunit of NMDA
                     receptor family; cd06378"
                     /db_xref="CDD:380601"
     Site            order(32,78..80,115,136,176,178,193,208..209,310..311)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:380601"
     Site            order(76..81,83..84,119..120,176,180,207,209,310..316,
                     320..322,333)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:380601"
     Region          403..802
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(511..514,516,518..520,666,688..689,693,731,756..758,
                     761,767,783..786,789)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(511..513,518,688..690,730..731)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     Region          839..1464
                     /region_name="NMDAR2_C"
                     /note="N-methyl D-aspartate receptor 2B3 C-terminus;
                     pfam10565"
                     /db_xref="CDD:463148"
     CDS             1..1464
                     /gene="Grin2a"
                     /gene_synonym="GluN2A; GluRepsilon1; NMDAR2A; NR2A"
                     /coded_by="NM_008170.4:1201..5595"
                     /db_xref="CCDS:CCDS27943.1"
                     /db_xref="GeneID:14811"
                     /db_xref="MGI:MGI:95820"
ORIGIN      
        1 mgrlgywtll vlpallvwhg paqnaaaekg tpalniavll ghshdvtere lrnlwgpeqa
       61 tglpldvnvv allmnrtdpk slithvcdlm sgarihglvf gddtdqeava qmldfissqt
      121 fipilgihgg asmimadkdp tstffqfgas iqqqatvmlk imqdydwhvf slvttifpgy
      181 rdfisfiktt vdnsfvgwdm qnvitldtsf edaktqvqlk kihssvilly cskdeavlil
      241 searslgltg ydffwivpsl vsgntelipk efpsglisvs yddwdyslea rvrdglgilt
      301 taassmlekf syipeakasc ygqtekpetp lhtlhqfmvn vtwdgkdlsf teegyqvhpr
      361 lvvivlnkdr ewekvgkwen qtlslrhavw pryksfsdce pddnhlsivt leeapfvive
      421 didpltetcv rntvpcrkfv kinnstnegm nvkkcckgfc idilkklsrt vkftydlylv
      481 tngkhgkkvn nvwngmigev vyqravmavg sltineerse vvdfsvpfve tgisvmvsrs
      541 ngtvspsafl epfsasvwvm mfvmllivsa iavfvfeyfs pvgynrnlak gkaphgpsft
      601 igkaiwllwg lvfnnsvpvq npkgttskim vsvwaffavi flasytanla afmiqeefvd
      661 qvtglsdkkf qrphdysppf rfgtvpngst ernirnnypy mhqymtkfnq rgvedalvsl
      721 ktgkldafiy daavlnykag rdegcklvti gsgyifattg ygialqkgsp wkrqidlall
      781 qfvgdgemee letlwltgic hneknevmss qldidnmagv fymlaaamal slitfiwehl
      841 fywklrfcft gvcsdrpgll fsisrgiysc ihgvhieekk kspdfnltgs qsnmlkllrs
      901 aknisnmsnm nssrmdspkr aadfiqrgsl ivdmvsdkgn liysdnrsfq gkdsifgenm
      961 nelqtfvanr hkdslsnyvf qgqhpltlne snpntvevav steskgnsrp rqlwkksmes
     1021 lrqdslnqnp vsqrdektae nrthslkspr ylpeevahsd isetssratc hrepdnnknh
     1081 ktkdnfkrsm askypkdcse vertyvktka ssprdkiyti dgekepsfhl dppqfieniv
     1141 lpenvdfpdt yqdhnenfrk gdstlpmnrn plhnedglpn ndqyklyakh ftlkdkgsph
     1201 segsdryrqn sthcrsclsn lptysghftm rspfkcdacl rmgnlydide dqmlqetgnp
     1261 atreeayqqd wsqnnalqfq knklkinrqh sydnildkpr eidlsrpsrs islkdrerll
     1321 egnlygslfs vpsskllgnk sslfpqgled skrsksllpd htsdnpflht ygddqrlvig
     1381 rcpsdpykhs lpsqavndsy lrsslrstas ycsrdsrghs dvyisehvmp yaanknnmys
     1441 tprvlnscsn rrvykkmpsi esdv
//
