LOCUS       NM_001083806           10057 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus glutamate receptor, ionotropic, AMPA2 (alpha 2)
            (Gria2), transcript variant 1, mRNA.
ACCESSION   NM_001083806
VERSION     NM_001083806.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Hong,I., Kim,J., Hainmueller,T., Kim,D.W., Keijser,J.,
            Johnson,R.C., Park,S.H., Limjunyawong,N., Yang,Z., Cheon,D.,
            Hwang,T., Agarwal,A., Cholvin,T., Krienen,F.M., McCarroll,S.A.,
            Dong,X., Leopold,D.A., Blackshaw,S., Sprekeler,H., Bergles,D.E.,
            Bartos,M., Brown,S.P. and Huganir,R.L.
  TITLE     Calcium-permeable AMPA receptors govern PV neuron feature
            selectivity
  JOURNAL   Nature 635 (8038), 398-405 (2024)
   PUBMED   39358515
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   Ratsika,A., Codagnone,M.G., Bastiaanssen,T.F.S., Hoffmann
            Sarda,F.A., Lynch,C.M.K., Ventura-Silva,A.P., Rosell-Cardona,C.,
            Caputi,V., Stanton,C., Fulling,C., Clarke,G. and Cryan,J.F.
  TITLE     Maternal high-fat diet-induced microbiota changes are associated
            with alterations in embryonic brain metabolites and adolescent
            behaviour
  JOURNAL   Brain Behav Immun 121, 317-330 (2024)
   PUBMED   39032541
REFERENCE   3  (bases 1 to 10057)
  AUTHORS   He,X., Hu,X.Y., Yin,X.Y., Wu,X.M., Liu,Q.R. and Shen,J.C.
  TITLE     GluR2 can Drive Neuroinflammation and Cognitive Impairments
            Following Peripherally Repeated Lipopolysaccharide Exposures
  JOURNAL   Neurochem Res 49 (9), 2393-2407 (2024)
   PUBMED   38837093
  REMARK    GeneRIF: GluR2 can Drive Neuroinflammation and Cognitive
            Impairments Following Peripherally Repeated Lipopolysaccharide
            Exposures.
REFERENCE   4  (bases 1 to 10057)
  AUTHORS   Lebedeva,M., Kubistova,A., Spisska,V., Filipovska,E., Pacesova,D.,
            Svobodova,I., Kuchtiak,V., Balik,A. and Bendova,Z.
  TITLE     The disruption of circadian rhythmicity of gene expression in the
            hippocampus and associated structures in Gria2R/R mice; a
            comparison with C57BL/6J and Adar2-/- mice strains
  JOURNAL   Brain Res 1826, 148739 (2024)
   PUBMED   38157956
  REMARK    GeneRIF: The disruption of circadian rhythmicity of gene expression
            in the hippocampus and associated structures in Gria2[R/R] mice; a
            comparison with C57BL/6J and Adar2[-/-] mice strains.
REFERENCE   5  (bases 1 to 10057)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 10057)
  AUTHORS   Brusa,R., Zimmermann,F., Koh,D.S., Feldmeyer,D., Gass,P.,
            Seeburg,P.H. and Sprengel,R.
  TITLE     Early-onset epilepsy and postnatal lethality associated with an
            editing-deficient GluR-B allele in mice
  JOURNAL   Science 270 (5242), 1677-1680 (1995)
   PUBMED   7502080
REFERENCE   7  (bases 1 to 10057)
  AUTHORS   McNamara,J.O., Eubanks,J.H., McPherson,J.D., Wasmuth,J.J.,
            Evans,G.A. and Heinemann,S.F.
  TITLE     Chromosomal localization of human glutamate receptor genes
  JOURNAL   J Neurosci 12 (7), 2555-2562 (1992)
   PUBMED   1319477
REFERENCE   8  (bases 1 to 10057)
  AUTHORS   Sakimura,K., Morita,T., Kushiya,E. and Mishina,M.
  TITLE     Primary structure and expression of the gamma 2 subunit of the
            glutamate receptor channel selective for kainate
  JOURNAL   Neuron 8 (2), 267-274 (1992)
   PUBMED   1310861
REFERENCE   9  (bases 1 to 10057)
  AUTHORS   Sommer,B., Kohler,M., Sprengel,R. and Seeburg,P.H.
  TITLE     RNA editing in brain controls a determinant of ion flow in
            glutamate-gated channels
  JOURNAL   Cell 67 (1), 11-19 (1991)
   PUBMED   1717158
  REMARK    GeneRIF: Gria2 transcripts undergo RNA editing.
REFERENCE   10 (bases 1 to 10057)
  AUTHORS   Sakimura,K., Bujo,H., Kushiya,E., Araki,K., Yamazaki,M.,
            Yamazaki,M., Meguro,H., Warashina,A., Numa,S. and Mishina,M.
  TITLE     Functional expression from cloned cDNAs of glutamate receptor
            species responsive to kainate and quisqualate
  JOURNAL   FEBS Lett 272 (1-2), 73-80 (1990)
   PUBMED   1699805
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC121908.3 and AC163349.3.
            
            On Apr 16, 2020 this sequence version replaced NM_001083806.2.
            
            Summary: Glutamate receptors are the predominant excitatory
            neurotransmitter receptors in the mammalian brain and are activated
            in a variety of normal neurophysiologic processes. This gene
            product belongs to a family of glutamate receptors that are
            sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate
            (AMPA), and function as ligand-activated cation channels. These
            channels are assembled from 4 related subunits, Gria1-4. The
            subunit encoded by this gene (Gria2) is subject to RNA editing
            (CAG->CGG; Q->R) within the second transmembrane domain, which is
            thought to render the channel impermeable to Ca(2+). Alternative
            splicing, resulting in transcript variants encoding different
            isoforms, (including the flip and flop isoforms that vary in their
            signal transduction properties), has been noted for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1 (also known
            as the flip isoform).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.178563.1,
                                           SRR1660819.131684.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            undergoes RNA editing  :: PMID: 1717158, 12045112
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-397               AC121908.3         36966-37362         c
            398-538             AC121908.3         36314-36454         c
            539-778             AC163349.3         210192-210431       c
            779-975             AC163349.3         201271-201467       c
            976-1029            AC163349.3         196065-196118       c
            1030-1191           AC163349.3         191265-191426       c
            1192-1359           AC163349.3         179910-180077       c
            1360-1464           AC163349.3         179523-179627       c
            1465-1575           AC163349.3         178611-178721       c
            1576-1782           AC163349.3         176932-177138       c
            1783-2153           AC163349.3         176121-176491       c
            2154-2352           AC163349.3         171691-171889       c
            2353-2600           AC163349.3         161518-161765       c
            2601-2715           AC163349.3         159637-159751       c
            2716-2964           AC163349.3         158336-158584       c
            2965-10057          AC163349.3         150855-157947       c
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 35.5 cM"
     gene            1..10057
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="glutamate receptor, ionotropic, AMPA2 (alpha 2)"
                     /db_xref="GeneID:14800"
                     /db_xref="MGI:MGI:95809"
     exon            1..397
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     CDS             310..2961
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; AMPA selective glutamate receptor; glutamate
                     receptor B; glutamate receptor 2; AMPA-selective glutamate
                     receptor 2; glutamate receptor ionotropic, AMPA 2"
                     /codon_start=1
                     /product="glutamate receptor 2 isoform 1 precursor"
                     /protein_id="NP_001077275.2"
                     /db_xref="CCDS:CCDS38459.1"
                     /db_xref="GeneID:14800"
                     /db_xref="MGI:MGI:95809"
                     /translation="MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAF
                     RVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITS
                     FCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLST
                     LQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVND
                     IVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIER
                     WSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANP
                     AVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEV
                     DKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLA
                     AEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREE
                     VIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSR
                     FSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGG
                     VWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRR
                     SKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCD
                     TMKVGGNLDSKGYGIATPKGSSLRTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKD
                     SGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNAQNIN
                     PSSSQNSQNFATYKEGYNVYGIESVKI"
     exon            398..538
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..778
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            779..975
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1029
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1191
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1359
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1464
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1575
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1782
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..2153
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2154..2352
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2600
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2715
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2964
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..10057
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3864..3869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="hexamer: AATAAA"
     polyA_site      3888
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="major polyA site"
     regulatory      6627..6632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="hexamer: ATTAAA"
     polyA_site      6647
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="major polyA site"
     polyA_site      6798
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
     regulatory      7057..7062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="hexamer: ATTAAA"
     polyA_site      7076
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
     regulatory      10029..10034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
                     /note="hexamer: ATTAAA"
     polyA_site      10057
                     /gene="Gria2"
                     /gene_synonym="GluA2; Glur-2; GluR-B; gluR-K2; Glur2"
ORIGIN      
        1 gattgtgtga gtgcacggga gggtgctgat attcccagac accgggacca cagcagcagc
       61 tcagctaaaa actgcattca gccagtcctc ggacttcagg agcagggaca ggacgcaggc
      121 atcaacagcc accagctaaa cctgggagat aaggattctt ctgccttcac ttcgtggttt
      181 tagcagctcc ttgctaaatt tcgacctcaa aatgcagagg atctaatttg ctgaggaaaa
      241 cggtcaaaga aggaaaagga ggaaagggaa acgaggggat attttgtgga tgctctactt
      301 ttcttggaaa tgcaaaagat tatgcatatt tctgtcctcc tttctcctgt tttatgggga
      361 ctgatttttg gtgtctcttc taacagcata cagatagggg ggctatttcc aaggggcgct
      421 gatcaagaat acagtgcatt tcgggtaggg atggttcagt tttccacttc ggagttcaga
      481 ctgacacccc atatcgacaa tttggaggta gccaacagtt tcgcagtcac caatgctttc
      541 tgctcccagt tttcaagagg cgtctatgcg atttttgggt tttacgacaa gaagtctgta
      601 aataccatca catcattctg tgggacactg catgtatcct tcatcacacc aagcttccca
      661 acagatggca cgcatccatt tgtcatccag atgcgacctg acctcaaagg agcactcctt
      721 agcttgattg agtactacca atgggataag ttcgcatacc tctatgacag tgacagaggc
      781 ttatcaacac tgcaagctgt gctggattct gctgcggaga agaagtggca ggtgactgct
      841 atcaatgtgg ggaacattaa caatgacaag aaagatgaga cctacagatc actctttcaa
      901 gatctggagt taaaaaaaga acggcgtgta atccttgact gcgaaaggga taaagtcaat
      961 gacattgtgg accaggttat taccattgga aagcatgtta aagggtacca ttatatcatt
     1021 gcaaatctgg gatttactga tggagacctg ctgaaaattc agtttggagg agcaaatgtc
     1081 tctggatttc agattgtaga ctacgacgac tccctggtgt ctaaatttat agaaagatgg
     1141 tcaacactcg aagagaaaga ataccctgga gcacacacag cgacaattaa gtatacttcg
     1201 gccctgactt atgatgctgt ccaagtgatg actgaagcat tccgcaatct tcggaagcag
     1261 aggattgaaa tctccaggag aggaaatgca ggagattgtt tggccaaccc agctgtgcct
     1321 tggggacaag gcgtggaaat agaaagggcc ctcaagcagg ttcaagttga aggtctctct
     1381 ggaaatataa aatttgacca gaacggaaaa cgaataaact acacaattaa catcatggag
     1441 ctcaaaacaa atggaccccg gaagattggg tactggagtg aagtggataa aatggttgtc
     1501 accctaaccg agctcccctc tggaaatgac acatctgggc ttgaaaacaa aactgtggtt
     1561 gtcaccacaa tattggaatc tccatatgtt atgatgaaga aaaatcatga aatgcttgaa
     1621 gggaatgagc gttatgaggg ctactgtgtt gacttagctg cagaaattgc caaacattgt
     1681 ggattcaagt acaagctgac tattgttggg gatggcaagt atggggccag ggatgcagac
     1741 accaaaattt ggaatggtat ggttggagaa cttgtatatg ggaaagctga tattgccatt
     1801 gctccattaa ctatcactct cgtgagagaa gaggtgattg acttctcgaa gccattcatg
     1861 agccttggaa tctctatcat gatcaagaag cctcagaagt ccaaaccagg agtgttttcc
     1921 tttcttgatc ctttagccta tgagatctgg atgtgcattg tgtttgccta cattggggtc
     1981 agtgtagttt tattcctggt cagcagattt agcccctacg agtggcacac tgaggaattt
     2041 gaagatggaa gagaaacaca aagtagtgaa tcaactaatg aatttgggat ttttaatagt
     2101 ctctggtttt ccttgggtgc ctttatgcag caaggatgcg atatttcgcc aagatctctc
     2161 tctgggcgca ttgttggagg tgtgtggtgg ttctttaccc tcatcatcat ctcctcctac
     2221 acggctaact tagctgcctt cctgactgta gagaggatgg tgtcgcccat cgaaagtgct
     2281 gaggatctgt ctaagcaaac agaaattgct tatggaacat tagactctgg ctccactaaa
     2341 gagtttttca ggagatctaa aattgcagtg tttgataaaa tgtggactta tatgaggagt
     2401 gcagagccct ctgtgtttgt gaggactacg gcagaaggag tagccagagt caggaaatcc
     2461 aaagggaagt atgcctactt gctggagtcc acaatgaatg agtacatcga gcagaggaag
     2521 ccttgcgaca ccatgaaagt gggcggcaac ctggattcca aaggctacgg catcgccaca
     2581 cctaaaggat cctcattaag aaccccagta aatcttgcag tattgaaact cagtgagcaa
     2641 ggcgtcttag acaagctgaa aaacaaatgg tggtacgata aaggtgaatg tggagccaag
     2701 gactcgggaa gtaaggaaaa gaccagtgcc ctcagtctga gcaacgttgc tggagtattc
     2761 tacatccttg tcgggggcct tggtttggca atgctggtgg ctttgattga gttctgttac
     2821 aagtcaaggg ccgaggcgaa acgaatgaag gtggcaaaga atgcacagaa tattaaccca
     2881 tcttcctcgc agaattccca gaattttgca acttataagg aaggttacaa cgtatatggc
     2941 atcgagagtg ttaaaattta ggggatgacc ttgagcgctg ccacgaggaa caaggcaagg
     3001 ctgtcaatta caggaagtac tggagaaaat ggacgtgtta tgactccaga atttcccaaa
     3061 gcagtgcatg ctgtccctta cgtgagtcct ggcatgggaa tgaatgtcag tgtgactgat
     3121 ctctcgtgat tgataggaac cttctgagtg ccttacacaa tggtttcctt gtgtgtttat
     3181 tgtcaaagtg gtgagaggca tccgatatct tgaaggcttt tctttcagcc aagaattctt
     3241 aactatgtgg agttcacctt gaattgtaag gaaagattaa ttacaaacag agcatccttt
     3301 tctacttcga tgtcagagga agcgtggtgg acatgcacag ctaacatgga agtactatca
     3361 cttaactgaa gtctttgtac agacaacaaa cccgtttccg cagccactat tgttagtctc
     3421 ttgattcata atgactaagc acacttgaca tcaactgcat caagatgtga cctgttttat
     3481 aaaaaaaaag aggaaaaaaa aacatttaaa ataaaaaaaa tatttttagg tattttcaca
     3541 gacaaactgg cttttaaata aatttgcttc cgtactggtt ggttaagaca aaaacaaaaa
     3601 caaaacaaaa caaaacaaaa aaaacaacaa aaaaacaaaa caaaacaaaa caaaaaaata
     3661 aagtcaaact gtgtgggaag tgatggggaa aatggtccat ggtatcagtt ccatattttt
     3721 caaagccaaa tatgtaaatg ctgaggaagg aaacagagga gactccagtc ttgtaattta
     3781 atattgttat taaaacttta atgtatcctg ttctttaaca tttggtgtta atatgaaact
     3841 acttggcgat gcttgacatt tgaaataaac attttttcta ttgttttatt tgcaagtggt
     3901 ccagttaatt ttgcttagct acagtttggt catacatcca gtgatctaaa gacactaatg
     3961 catagtcctt aggatcccct aagaaaaaga ccccagttaa aatacaggaa tagtttttct
     4021 ttgacaaata gaatttaccc caaatagtat atctaaaact attttttctg tacatcatat
     4081 atataatagt aaaatatttg ttaattaaaa agcattaatc tatatagata aatgcttgaa
     4141 gatttaaaaa tcagatttat tatcaaatgt cagattgtgt gattttctgc tcttcaagta
     4201 tttaaggttg tgtaggagca gcaatcatta tatataaagt gaaaaaggct tgaaggtagc
     4261 tgttcagata cttatacact ggccaaaatc tataatctaa gtatgtattt ttagttttaa
     4321 aattataaga actgacatca gtttttgaat actatagtca cctacaaata gtttctttcc
     4381 acttctatct ataaaatcaa ctctaatata ttaattctta tttatgcctc aagtagaagg
     4441 agcactttaa tttgtgtaat gtggatactg ttccatctct gtaactttcc actaaagaaa
     4501 tcatactaca ctatttgatt catttaagcc atctatcttc ccttccttaa aatatctttt
     4561 atgtttattt ccactaatgt cctccaaata aaattacttt agagttaaaa gcaactgaca
     4621 cccagtagtt atctttaaga tttatatgtt atttttctct cataaaaaat ctgtgtaata
     4681 tttgatgtct gaccaagtgc ttggtagagc atccttactg actattttta aggatcagaa
     4741 atgtaagatt taacagtagc cacttgcatt ctacatggta acatgtagtt tcatgatgat
     4801 cttcactaaa ttttaccatc ccttctataa attccaagat catacaaaat aatttgagtg
     4861 cattactcat cagggattct ggaggtacat tttaagtttt aataataaat gctagaatga
     4921 ccaaattgca gactaattat ttccatattg tacttaaaat gagtttttaa aaattatttt
     4981 aaaagagatg actatataca atcaaagcta tttattgtac ctctgggcct actcttccaa
     5041 aaattgagct tatcaatttt tctctgtcaa gcttgaacta atgtaaataa ttggaataat
     5101 gtaaagttat attttcatgt ttttatagtt atgacaagaa cacttaatgt aagagtatct
     5161 cgagtatgaa taatgttgat tggctaacac agagctcagt tttaggcagt aaaatctagt
     5221 tcaatatcca tgtaacaatg catcagtata ttgctgtata gatgtgtctg tgtgcatata
     5281 agtgataagt gctcaacaaa gagtggtgac agctctctca aggttttttt tttaattcat
     5341 tttctataaa ctgttatgga aagaccaaaa taattatgaa ctattcttat gtaaatttac
     5401 aattgtcctt tactgtacat tttgtttaca gtatagtacc ttattctctg ctgtgttaag
     5461 tgggtgtcat actccaagga gacaaacact ttctacaact tctattgaag attctgggat
     5521 ttccaatttt tcatgtgtac tatgtctgaa aatgcttttg attttatttt taaatctaac
     5581 atctgatgga tttttcagag tgttgtgaaa ccttcagtca tacataaaat atgttcttat
     5641 aaaatgtcaa catcttttta ttttgttttt acttaatggt atatcaatct aataatgaga
     5701 aaccaaaagt gtatcatata tccccccccc caaatctaga tgtttttagt gagggagggc
     5761 atcagtttgt cagcaaagtc tgaatagtgt caaggcagaa tattttttaa tttaaattaa
     5821 gagaattagt aattctggta tttcacacct atactcatta ggttctttgg acatatatag
     5881 aaatgtggct attgattaaa tcacttattt tgtaatagac aacactggta acataccagg
     5941 cattgtgcac ttagccctcc tactgtgcag tcatctcatt tgctgtggta ctcatattaa
     6001 agagcaggaa agtaacttgc tttaaaatat ggtttgactt ttaaaaattg accacttatt
     6061 atttaactta aaaattcttt ccactttaac taatttcttc acaaagctgg tcatttgaat
     6121 aactacacaa tcctatttat atgtacttta aagttattcc aatattatag ttctcctaac
     6181 ttgaacataa atttgtaaaa atttttatac ttaaatgtat caagtactta acacaccaat
     6241 ataaaggtga ttctgcttaa aaatacagga attttacatt tctaatctgt aaatactgtg
     6301 gatattttca tgatcaaggt agtactttga gaataagaag aaaattacta gcaacattcc
     6361 tgtaaaaacc tttagcacct accatatctt aaggatatca ttaactttta attttaaatg
     6421 aagtgtatgt atgtgcgtgc tgatgtggga atatgcccat gagtgcaggt gtctgctgat
     6481 aacaggggtg ggcccaggta tatcagagct agagtggggt tatagatgct tgccagctcc
     6541 taaaatggtt tttggagaaa ctagcctggg tccactgcaa gtgttctttc ttaaccactg
     6601 agcaatcctc cagcaccaat aacatcatta aaacttttta aaaagaacct ctttgcagtt
     6661 cattaaacta tgacatacca atttttcata ctttacctaa tggcaatctg gaggtccatc
     6721 tacataatag aaactattcg atgacttcac gtgtggaatg attttctgtg tttttaaact
     6781 tgctttctgc tgataaaatt gattgtgcat gggttttttt tttcttgagg gggggcgggg
     6841 agggtggtga gtcctctgaa agaaagtgca attaggtcaa atgtatcttt ctatttaatt
     6901 tatatgttat atgcaggtgt ttgatcgaat gacttagaat gtttataaca ctagcattag
     6961 ttggaaatat atacattaat aagagtatag tattaacttt ggacacttga ttaggtaagc
     7021 ttgacaaatc tttatgaata gattcaaaag aagcatatta aagaagttgc ctgaaagttg
     7081 caaatatgca ttaactatgg aaagatgtac aaggtgctct gacacttaaa actggtttaa
     7141 tttacctcgc cggcacggag gatgggtaag tatacacaaa ctgctctgag accctgaaca
     7201 aaagttgagg gagcactaga tggtttggga aatattgaat taaacctttt ttaaaacatg
     7261 cttttaatat attgtttggg ttaaaccatg acacaagaca ttttgttaga cactcaataa
     7321 ttacacttta tgtttaaaaa gaacaaccca cgaacagcac acaacactcc aagcccatgg
     7381 aggttcaaat acatttatgt aaacataaca tctcagaaag tttccacaag ctctaaagaa
     7441 gtacccagag ctaatgtgga tcatgaagaa tttgtgaatg tcttgtgtcc tgtctgtatc
     7501 cctttcttgc tttatatttt acactctctt tgtaccctta gatacaagtg acatttgtca
     7561 cttaacatat ttccaatacc atactttctc agtgtatagt ttcatccatc ttatttcatt
     7621 gcatccctcc ctcacactgt ccatctagct ttgactttcc ttatgagaaa ttttgctttg
     7681 agaacacgta caatgcagtt cacttcgtcc aagacaggac tgaaactttc aaagtctttt
     7741 taatgaccat agcaaggaac taggaatctc tcaaacgaaa gaggagagca tgtgttaatc
     7801 tccatgtttg catcacacac gtattctgat ccaacatggc tgcttagcta tatacccatt
     7861 tcacctaaag actccacatt caggagagtt cctggaggat gcaggaggtt ctctgaagaa
     7921 gcagaaaacc tgagaactca gtcttatctt tgcccatcag ggttttgata gccacttcac
     7981 tagaagtaca acctattctc tattttaatt caattcccta ggattttgaa atttatatct
     8041 ctcacaaagt ataattataa cctaattttg ctattccaca gccaaactcc ttttggatat
     8101 gcataaactc agaattattt aattaatgat aacctggatt tactgaataa atttggtcac
     8161 ttttctttct ttctttttcc tttcaggtta atgtgccaat ttcattttac tgtaaattgg
     8221 aatgtggggg agtggattaa actcttggga gggagtcaag tccagctctc tagctttatc
     8281 ttccccatcc cctcacctct gtgtgtctcc tcaactggag tatgtggata ctaggacagt
     8341 ttcccggaat gatgaaagga aagactgtat cttttaaaat tgatgcttga agaaacaggt
     8401 tcttgcatgg gagtgccact cagtgcagaa agttttgtta tttggttcaa aatggttttt
     8461 atcatcttaa cacgctagtc agttttatct gcgaacattc attcagtaac tcactaattt
     8521 acccatttgc tcaaaaaaat gcttattgaa tctattaaat agtatttatt agaggtaagg
     8581 gctaaattta aagcctctat ttctgagaag tttctataaa tcatgctctg atgtgttccc
     8641 agccacatag aaataatttg gttttagggt tagtatctga caattaaaat tggtatattt
     8701 acctgttctg tagaagacat tgtactttcc ttcaggacaa aagtcttggc aacattcatg
     8761 agaaattgcc tctaacatgg agtcataatt tcctctcaaa aaagcttata tttggtaaat
     8821 tgcttcccta taatcacaaa ttctcttctc tactctcaaa aagtcaaatt cagttcatca
     8881 acaaacacca cagtttgatg atgctaacct ctgcctccta catagcatag tttacatgca
     8941 cctaaaatcc atttcaatga taagaaaata agaaaacttt agatgaaatt taagtagcaa
     9001 cagggaattt cccaaccaag tacagaacaa tttcgaattt atcaacggaa gtggttaaaa
     9061 acaagtatct gtatttatta ttgtttaatt tattttattt gcattaagta agacataaac
     9121 tcaaagcaaa tagtttgatt tgtgtgagaa atgttttaga tgaattcata tatatatata
     9181 tacacacata tgtataggag tgtacatgtt aaagtcaatt attttaatca atacataatt
     9241 cattatgcca ccagcagtag gcaaagaaac acatttgcta ggcatcagaa tcactctcaa
     9301 ttacaaatta agaaatgtat attttacatt tatttttatt tgactttcat aatataattt
     9361 ataggttttt caggtatcta taatattttt tacagcatct tttggtcaag aattttctga
     9421 tacaaaattc ctattcttct aggagtttta tgatttaagc tttggatgta ctggattcct
     9481 accgtctgaa gcacttctgt gtaaattcag agttcaaaat agcccagtgg gagtggttag
     9541 caagtttctt caaacaatat gtagcatatt gaagtcaaca gtactcttac tttgcctctg
     9601 ccttctatcc ttcattctcc accctgtccc agctccacat caaaccctgt ttggatgctt
     9661 ggtgaagaca tacattgtac ttttattcat ctaaatctca tggcttgtag tgaggcatag
     9721 caattttcct gggatttcta ggtcatagtt ggcatagtac ttcctgcaca tttttcttac
     9781 acatctgtgc cttcgttttt cagtggagta aatgcaggct atctgtattg gcctcacgtc
     9841 tatttaggtt atttttaaat taaaatgtaa ttttggactt atacctaaac tcagtctgta
     9901 tctgtaaagg tttacaagcg tgtttacatg cgttattgtg ttagctattt gtgttaaaga
     9961 gataaacatg tctgttcatt ttaatttaat gtttttgtgt gtttctctgt gtgtatgttt
    10021 atgtgtgtat taaaaatatt ccatatcttc tgtagta
//
