LOCUS       NM_008163               2725 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus growth factor receptor bound protein 2 (Grb2),
            transcript variant 1, mRNA.
ACCESSION   NM_008163
VERSION     NM_008163.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Zhao,H. and Mao,H.
  TITLE     ERRFI1 exacerbates hepatic ischemia reperfusion injury by promoting
            hepatocyte apoptosis and ferroptosis in a GRB2-dependent manner
  JOURNAL   Mol Med 30 (1), 82 (2024)
   PUBMED   38862918
  REMARK    GeneRIF: ERRFI1 exacerbates hepatic ischemia reperfusion injury by
            promoting hepatocyte apoptosis and ferroptosis in a GRB2-dependent
            manner.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Ye,Z., Xu,S., Shi,Y., Cheng,X., Zhang,Y., Roy,S., Namjoshi,S.,
            Longo,M.A., Link,T.M., Schlacher,K., Peng,G., Yu,D., Wang,B.,
            Tainer,J.A. and Ahmed,Z.
  TITLE     GRB2 stabilizes RAD51 at reversed replication forks suppressing
            genomic instability and innate immunity against cancer
  JOURNAL   Nat Commun 15 (1), 2132 (2024)
   PUBMED   38459011
  REMARK    GeneRIF: GRB2 stabilizes RAD51 at reversed replication forks
            suppressing genomic instability and innate immunity against cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2725)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2725)
  AUTHORS   Li,X., Liu,C., Zhang,Z., Li,X., Yao,Z., Dong,Y., Wang,X. and
            Chen,Z.
  TITLE     Hepatocyte-specific Wtap deficiency promotes hepatocellular
            carcinoma by activating GRB2-ERK depending on downregulation of
            proteasome-related genes
  JOURNAL   J Biol Chem 299 (11), 105301 (2023)
   PUBMED   37777158
  REMARK    GeneRIF: Hepatocyte-specific Wtap deficiency promotes
            hepatocellular carcinoma by activating GRB2-ERK depending on
            downregulation of proteasome-related genes.
REFERENCE   5  (bases 1 to 2725)
  AUTHORS   Huang,Y., Zhao,H., Shi,X., Liu,J., Lin,J.M., Ma,Q., Jiang,S.,
            Pu,W., Ma,Y., Liu,J., Wu,W., Wang,J. and Liu,Q.
  TITLE     GRB2 serves as a viable target against skin fibrosis in systemic
            sclerosis by regulating endothelial cell apoptosis
  JOURNAL   J Dermatol Sci 111 (3), 109-119 (2023)
   PUBMED   37661474
  REMARK    GeneRIF: GRB2 serves as a viable target against skin fibrosis in
            systemic sclerosis by regulating endothelial cell apoptosis.
REFERENCE   6  (bases 1 to 2725)
  AUTHORS   Wang,X.Y., Fuhrer,D.K., Marshall,M.S. and Yang,Y.C.
  TITLE     Interleukin-11 induces complex formation of Grb2, Fyn, and JAK2 in
            3T3L1 cells
  JOURNAL   J Biol Chem 270 (47), 27999-28002 (1995)
   PUBMED   7499280
REFERENCE   7  (bases 1 to 2725)
  AUTHORS   Huang,L., Turck,C.W., Rao,P. and Peters,K.G.
  TITLE     GRB2 and SH-PTP2: potentially important endothelial signaling
            molecules downstream of the TEK/TIE2 receptor tyrosine kinase
  JOURNAL   Oncogene 11 (10), 2097-2103 (1995)
   PUBMED   7478529
REFERENCE   8  (bases 1 to 2725)
  AUTHORS   den Hertog,J., Tracy,S. and Hunter,T.
  TITLE     Phosphorylation of receptor protein-tyrosine phosphatase alpha on
            Tyr789, a binding site for the SH3-SH2-SH3 adaptor protein GRB-2 in
            vivo
  JOURNAL   EMBO J 13 (13), 3020-3032 (1994)
   PUBMED   7518772
REFERENCE   9  (bases 1 to 2725)
  AUTHORS   Rozakis-Adcock,M., McGlade,J., Mbamalu,G., Pelicci,G., Daly,R.,
            Li,W., Batzer,A., Thomas,S., Brugge,J., Pelicci,P.G.,
            Schlessinger,J., Pawson,T. et al.
  TITLE     Association of the Shc and Grb2/Sem5 SH2-containing proteins is
            implicated in activation of the Ras pathway by tyrosine kinases
  JOURNAL   Nature 360 (6405), 689-692 (1992)
   PUBMED   1465135
REFERENCE   10 (bases 1 to 2725)
  AUTHORS   Lowenstein,E.J., Daly,R.J., Batzer,A.G., Li,W., Margolis,B.,
            Lammers,R., Ullrich,A., Skolnik,E.Y., Bar-Sagi,D. and
            Schlessinger,J.
  TITLE     The SH2 and SH3 domain-containing protein GRB2 links receptor
            tyrosine kinases to ras signaling
  JOURNAL   Cell 70 (3), 431-442 (1992)
   PUBMED   1322798
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC052377.1, AK155800.1 and
            BQ174314.1.
            
            On Sep 15, 2015 this sequence version replaced NM_008163.3.
            
            Summary: The protein encoded by this gene binds the epidermal
            growth factor receptor and contains one SH2 domain and two SH3
            domains. Its two SH3 domains direct complex formation with
            proline-rich regions of other proteins, and its SH2 domain binds
            tyrosine phosphorylated sequences. This gene is similar to the Sem5
            gene of C.elegans, which is involved in the signal transduction
            pathway. Three alternatively spliced transcript variants encoding
            the same protein have been found for this gene. [provided by
            RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. All three variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166142.1, AK172002.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1689              BC052377.1         1-1689
            1690-2155           AK155800.1         1646-2111
            2156-2725           BQ174314.1         1-570               c
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 80.91 cM"
     gene            1..2725
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /note="growth factor receptor bound protein 2"
                     /db_xref="GeneID:14784"
                     /db_xref="MGI:MGI:95805"
     exon            1..204
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     exon            205..409
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..985
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /note="SH2/SH3 adapter GRB2; adapter protein GRB2"
                     /codon_start=1
                     /product="growth factor receptor-bound protein 2"
                     /protein_id="NP_032189.1"
                     /db_xref="CCDS:CCDS25645.1"
                     /db_xref="GeneID:14784"
                     /db_xref="MGI:MGI:95805"
                     /translation="MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGK
                     DGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFG
                     NDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQQPTY
                     VQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV"
     exon            410..507
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     exon            508..630
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     exon            631..799
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     exon            800..2709
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2686..2691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /note="hexamer: AATAAA"
     polyA_site      2709
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /note="major polyA site"
ORIGIN      
        1 cgggaccctg gcgaggagcg gaagctaggt tgctctgttg cttctgcgat cgggcggcgg
       61 cggcggcgag agcgagcggg gctgcggccg ggacggcggt cggggcagcg ggcgccgcag
      121 tcagggagca gcagcgaggt tggcggctgc gggcggcagc gcagggcggg ccgggccggg
      181 tgacggagtg gacggcgcgt cacgggtggc attgtgtgtc ccagtgtgca agaatagccc
      241 cagaagagga aaggctgagc ccagagctct tcagcaggga agattccctt cccccctgct
      301 tcaggctgct gagcactgag cggcgctcag aatggaagcc atcgccaaat atgacttcaa
      361 agctactgct gacgatgagc tgagcttcaa aaggggggac atccttaagg ttttgaatga
      421 agaatgtgac cagaactggt ataaggcaga actcaatggg aaagatggct tcatccccaa
      481 gaattacata gaaatgaaac cacatccgtg gttttttggc aaaatcccca gagccaaggc
      541 agaagaaatg ctcagcaaac agcggcatga cggggccttc ctgatccgag agagcgagag
      601 cgctcctggg gacttctccc tgtccgtcaa gtttggaaat gatgtgcagc acttcaaggt
      661 gctccgcgac ggagccggga agtatttcct gtgggtggtg aagtttaatt ctttgaatga
      721 gctggtagat taccacagat caacatccgt gtccaggaac cagcagatat tcttacggga
      781 catagaacag atgccacagc agccaaccta cgtccaggcg ctctttgact ttgaccccca
      841 ggaggatggc gagctgggct ttcgcagagg agacttcatt catgtcatgg ataactcaga
      901 tcccaattgg tggaaagggg cctgccacgg gcagaccggc atgtttcccc gcaattatgt
      961 caccccagtg aaccggaacg tctaagaagc aaaagagatt atttaaagaa agtgaaaagt
     1021 taagaccgtt cacaagaatt acacccacac gctgcctgtc acagcctgtg agggaacgca
     1081 gaacacctgg ctgggtccca cgggtgaccc tctcattggg ttgcaacttt ggggggtggg
     1141 gaggggtgtt tgatttcata atgccaaaac ttaacctatt gaatgaatta cagtttttat
     1201 tacggaatct cgccgctacc cctgttcccc tcctgtgtcc tttttctcgt tctttctttc
     1261 ctgtccagtg catgatgttt aaggccacat atagtccagc tgatgccaat aataaaagac
     1321 aagaaaccaa gtgggctggt attttctcta tgcaaaatgt ctgtggagat ggatggactg
     1381 aaagagccgg attcctcaca cacaggggca gccagtgctt ctggggccct ggttggggtt
     1441 cacccgagat gcccaggggt accgcctcca gcctcaggcc tggagcattc catcaaagtt
     1501 ggaattaggg gaaggaggcc cactgtcctc cccggtctcc tgagagtcag actgcaggcc
     1561 ctcccctctc ccactgcttc ccttcaggtg ttttgacgtt tttttgtttg tttgtttgtt
     1621 ttttttaaat agtgcctttg tcttatttca agggtgttca taaatggtat ttgtaccatt
     1681 ttttttttta ataagttaaa gacagtccag agcttttcag ttgattcgtc tcctatcctg
     1741 tgtaaatatt ttcctctcag ggcaggggaa agaggacaga gaaaggagct ggtagaagca
     1801 gagagtgtat ttcccatctt gaatgggccg gaggtctcga agcctcagct tttactttgt
     1861 gagctgcaac actcgcttca gctcagactc agtggacatc agagtctctg tctccgtgtc
     1921 tcagttatgg tctgctctct ctatgcctgg agctactgat ccaaacacaa gacggtcaga
     1981 ggagccctgg catccttcac tcttataggc ctacatgcag atgggcttga agagaattgg
     2041 cctttcattt ttcacgctcg tatttcccca cctgtccaag ggtgcagata gcaggattgt
     2101 ggggtcggcc gcatctctct tggcagtggg ttgtgctgcc cctcccccca ccccacccct
     2161 ccatcccaca aagcctttcg aggtaggaga gaccaagacc agtgcacagc tttttaactc
     2221 ttgtcttctg tgagttttcc gtcctctgta gtcacgtgcc tgcacaccgt tctctccacc
     2281 ctgcctcctt cccacagcag aagcagggct ccacccaggc cttcccttgg agtcggtgca
     2341 tccatgggct gctagactct tgcgggtaga gtctcccctg gacttagcat tgtgagatgg
     2401 actcagctca gggcgcccct aaggctcgga ggcggccttc tgggtcccca cctctcctgg
     2461 ttccgctgct gctctccctg ctgatgataa agtaatctct ggagtcacac ctgggccatg
     2521 tgattgtttt attttggaat tggtgtatat catgaagcct tgctgaacta agttttgtgt
     2581 gtatatattt aaagatcagt gtttaaataa aaaaaaaaaa aaaaccaact atgtacttac
     2641 tctgacttct gcggacagca atttggtagg tagtgattaa ctgtgaataa acgtacaatg
     2701 agttcttaaa aaaaaaaaaa aaaaa
//
