LOCUS       NP_032189                217 aa            linear   ROD 06-MAY-2025
DEFINITION  growth factor receptor-bound protein 2 [Mus musculus].
ACCESSION   NP_032189
VERSION     NP_032189.1
DBSOURCE    REFSEQ: accession NM_008163.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 217)
  AUTHORS   Tran,D.N., Hwang,Y.J., Kim,K.C., Li,R., Marquardt,R.M., Chen,C.,
            Young,S.L., Lessey,B.A., Kim,T.H., Cheon,Y.P. and Jeong,J.W.
  TITLE     GRB2 regulation of essential signaling pathways in the endometrium
            is critical for implantation and decidualization
  JOURNAL   Nat Commun 16 (1), 2192 (2025)
   PUBMED   40038241
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 217)
  AUTHORS   Zhao,H. and Mao,H.
  TITLE     ERRFI1 exacerbates hepatic ischemia reperfusion injury by promoting
            hepatocyte apoptosis and ferroptosis in a GRB2-dependent manner
  JOURNAL   Mol Med 30 (1), 82 (2024)
   PUBMED   38862918
  REMARK    GeneRIF: ERRFI1 exacerbates hepatic ischemia reperfusion injury by
            promoting hepatocyte apoptosis and ferroptosis in a GRB2-dependent
            manner.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 217)
  AUTHORS   Ye,Z., Xu,S., Shi,Y., Cheng,X., Zhang,Y., Roy,S., Namjoshi,S.,
            Longo,M.A., Link,T.M., Schlacher,K., Peng,G., Yu,D., Wang,B.,
            Tainer,J.A. and Ahmed,Z.
  TITLE     GRB2 stabilizes RAD51 at reversed replication forks suppressing
            genomic instability and innate immunity against cancer
  JOURNAL   Nat Commun 15 (1), 2132 (2024)
   PUBMED   38459011
  REMARK    GeneRIF: GRB2 stabilizes RAD51 at reversed replication forks
            suppressing genomic instability and innate immunity against cancer.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 217)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (residues 1 to 217)
  AUTHORS   Li,X., Liu,C., Zhang,Z., Li,X., Yao,Z., Dong,Y., Wang,X. and
            Chen,Z.
  TITLE     Hepatocyte-specific Wtap deficiency promotes hepatocellular
            carcinoma by activating GRB2-ERK depending on downregulation of
            proteasome-related genes
  JOURNAL   J Biol Chem 299 (11), 105301 (2023)
   PUBMED   37777158
  REMARK    GeneRIF: Hepatocyte-specific Wtap deficiency promotes
            hepatocellular carcinoma by activating GRB2-ERK depending on
            downregulation of proteasome-related genes.
REFERENCE   6  (residues 1 to 217)
  AUTHORS   Wang,X.Y., Fuhrer,D.K., Marshall,M.S. and Yang,Y.C.
  TITLE     Interleukin-11 induces complex formation of Grb2, Fyn, and JAK2 in
            3T3L1 cells
  JOURNAL   J Biol Chem 270 (47), 27999-28002 (1995)
   PUBMED   7499280
REFERENCE   7  (residues 1 to 217)
  AUTHORS   Huang,L., Turck,C.W., Rao,P. and Peters,K.G.
  TITLE     GRB2 and SH-PTP2: potentially important endothelial signaling
            molecules downstream of the TEK/TIE2 receptor tyrosine kinase
  JOURNAL   Oncogene 11 (10), 2097-2103 (1995)
   PUBMED   7478529
REFERENCE   8  (residues 1 to 217)
  AUTHORS   den Hertog,J., Tracy,S. and Hunter,T.
  TITLE     Phosphorylation of receptor protein-tyrosine phosphatase alpha on
            Tyr789, a binding site for the SH3-SH2-SH3 adaptor protein GRB-2 in
            vivo
  JOURNAL   EMBO J 13 (13), 3020-3032 (1994)
   PUBMED   7518772
REFERENCE   9  (residues 1 to 217)
  AUTHORS   Rozakis-Adcock,M., McGlade,J., Mbamalu,G., Pelicci,G., Daly,R.,
            Li,W., Batzer,A., Thomas,S., Brugge,J., Pelicci,P.G.,
            Schlessinger,J., Pawson,T. et al.
  TITLE     Association of the Shc and Grb2/Sem5 SH2-containing proteins is
            implicated in activation of the Ras pathway by tyrosine kinases
  JOURNAL   Nature 360 (6405), 689-692 (1992)
   PUBMED   1465135
REFERENCE   10 (residues 1 to 217)
  AUTHORS   Lowenstein,E.J., Daly,R.J., Batzer,A.G., Li,W., Margolis,B.,
            Lammers,R., Ullrich,A., Skolnik,E.Y., Bar-Sagi,D. and
            Schlessinger,J.
  TITLE     The SH2 and SH3 domain-containing protein GRB2 links receptor
            tyrosine kinases to ras signaling
  JOURNAL   Cell 70 (3), 431-442 (1992)
   PUBMED   1322798
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC052377.1, AK155800.1 and
            BQ174314.1.
            
            Summary: The protein encoded by this gene binds the epidermal
            growth factor receptor and contains one SH2 domain and two SH3
            domains. Its two SH3 domains direct complex formation with
            proline-rich regions of other proteins, and its SH2 domain binds
            tyrosine phosphorylated sequences. This gene is similar to the Sem5
            gene of C.elegans, which is involved in the signal transduction
            pathway. Three alternatively spliced transcript variants encoding
            the same protein have been found for this gene. [provided by
            RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. All three variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166142.1, AK172002.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 80.91 cM"
     Protein         1..217
                     /product="growth factor receptor-bound protein 2"
                     /note="SH2/SH3 adapter GRB2; adapter protein GRB2"
                     /calculated_mol_wt=25107
     Region          1..56
                     /region_name="SH3_GRB2_N"
                     /note="N-terminal Src homology 3 domain of Growth factor
                     receptor-bound protein 2; cd11946"
                     /db_xref="CDD:212879"
     Site            order(1,37,50,53..55)
                     /site_type="other"
                     /note="C-terminal SH3 interface [polypeptide binding]"
                     /db_xref="CDD:212879"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     Site            order(6..9,12..13,15..16,31..33,35..36,49..52)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212879"
     Site            6
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     Site            50
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     Region          56..150
                     /region_name="SH2_Grb2_like"
                     /note="Src homology 2 domain found in Growth factor
                     receptor-bound protein 2 (Grb2) and similar proteins;
                     cd09941"
                     /db_xref="CDD:199828"
     Site            order(67,86,88,96,107,109)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:199828"
     Site            109
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     Region          160..212
                     /region_name="SH3_GRB2_C"
                     /note="C-terminal Src homology 3 domain of Growth factor
                     receptor-bound protein 2; cd11949"
                     /db_xref="CDD:212882"
     Site            order(165,167..168,170..171,192..193,206,208..209)
                     /site_type="other"
                     /note="Vav SH3 interface [polypeptide binding]"
                     /db_xref="CDD:212882"
     Site            order(165,167,170..171,174,190,192..193,204,206,208..209)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212882"
     Site            order(184..188,195,202)
                     /site_type="other"
                     /note="N-terminal SH3 interface [polypeptide binding]"
                     /db_xref="CDD:212882"
     Site            209
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     Site            211
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P62993; propagated from
                     UniProtKB/Swiss-Prot (Q60631.1)"
     CDS             1..217
                     /gene="Grb2"
                     /gene_synonym="Ash"
                     /coded_by="NM_008163.4:332..985"
                     /db_xref="CCDS:CCDS25645.1"
                     /db_xref="GeneID:14784"
                     /db_xref="MGI:MGI:95805"
ORIGIN      
        1 meaiakydfk ataddelsfk rgdilkvlne ecdqnwykae lngkdgfipk nyiemkphpw
       61 ffgkipraka eemlskqrhd gaflireses apgdfslsvk fgndvqhfkv lrdgagkyfl
      121 wvvkfnslne lvdyhrstsv srnqqiflrd ieqmpqqpty vqalfdfdpq edgelgfrrg
      181 dfihvmdnsd pnwwkgachg qtgmfprnyv tpvnrnv
//
