LOCUS       NM_001289442            5380 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus GRB2-related adaptor protein 2 (Grap2), transcript
            variant 2, mRNA.
ACCESSION   NM_001289442 XM_006520559
VERSION     NM_001289442.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Chougule,R.A., Cordero,E., Moharram,S.A., Pietras,K., Ronnstrand,L.
            and Kazi,J.U.
  TITLE     Expression of GADS enhances FLT3-induced mitogenic signaling
  JOURNAL   Oncotarget 7 (12), 14112-14124 (2016)
   PUBMED   26895103
  REMARK    GeneRIF: High GADS expression is associated with neoplasms.
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Dufner,A., Kisser,A., Niendorf,S., Basters,A., Reissig,S.,
            Schonle,A., Aichem,A., Kurz,T., Schlosser,A., Yablonski,D.,
            Groettrup,M., Buch,T., Waisman,A., Schamel,W.W., Prinz,M. and
            Knobeloch,K.P.
  TITLE     The ubiquitin-specific protease USP8 is critical for the
            development and homeostasis of T cells
  JOURNAL   Nat Immunol 16 (9), 950-960 (2015)
   PUBMED   26214742
REFERENCE   3  (bases 1 to 5380)
  AUTHORS   Xiong,J., Parker,B.L. and Yankee,T.M.
  TITLE     The combined loss of Gads and CD127 reveals a novel function of
            Gads prior to TCRbeta expression
  JOURNAL   Immunol Res 60 (1), 77-84 (2014)
   PUBMED   25037454
  REMARK    GeneRIF: The combined loss of Gads and CD127 reveals a novel
            function of Gads prior to T cell receptor beta expression
REFERENCE   4  (bases 1 to 5380)
  AUTHORS   Roncagalli,R., Hauri,S., Fiore,F., Liang,Y., Chen,Z., Sansoni,A.,
            Kanduri,K., Joly,R., Malzac,A., Lahdesmaki,H., Lahesmaa,R.,
            Yamasaki,S., Saito,T., Malissen,M., Aebersold,R., Gstaiger,M. and
            Malissen,B.
  TITLE     Quantitative proteomics analysis of signalosome dynamics in primary
            T cells identifies the surface receptor CD6 as a Lat
            adaptor-independent TCR signaling hub
  JOURNAL   Nat Immunol 15 (4), 384-392 (2014)
   PUBMED   24584089
REFERENCE   5  (bases 1 to 5380)
  AUTHORS   Gillis,L.C., Berry,D.M., Minden,M.D., McGlade,C.J. and Barber,D.L.
  TITLE     Gads (Grb2-related adaptor downstream of Shc) is required for
            BCR-ABL-mediated lymphoid leukemia
  JOURNAL   Leukemia 27 (8), 1666-1676 (2013)
   PUBMED   23399893
  REMARK    GeneRIF: GADS mediates lymphoid disease downstream of BCR-ABL
            through the recruitment of specific signaling intermediates.
REFERENCE   6  (bases 1 to 5380)
  AUTHORS   Yoder,J., Pham,C., Iizuka,Y.M., Kanagawa,O., Liu,S.K., McGlade,J.
            and Cheng,A.M.
  TITLE     Requirement for the SLP-76 adaptor GADS in T cell development
  JOURNAL   Science 291 (5510), 1987-1991 (2001)
   PUBMED   11239162
REFERENCE   7  (bases 1 to 5380)
  AUTHORS   Ellis,J.H., Ashman,C., Burden,M.N., Kilpatrick,K.E., Morse,M.A. and
            Hamblin,P.A.
  TITLE     GRID: a novel Grb-2-related adapter protein that interacts with the
            activated T cell costimulatory receptor CD28
  JOURNAL   J Immunol 164 (11), 5805-5814 (2000)
   PUBMED   10820259
REFERENCE   8  (bases 1 to 5380)
  AUTHORS   Law,C.L., Ewings,M.K., Chaudhary,P.M., Solow,S.A., Yun,T.J.,
            Marshall,A.J., Hood,L. and Clark,E.A.
  TITLE     GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to
            regulate nuclear factor of activated T cell activation
  JOURNAL   J Exp Med 189 (8), 1243-1253 (1999)
   PUBMED   10209041
REFERENCE   9  (bases 1 to 5380)
  AUTHORS   Liu,S.K. and McGlade,C.J.
  TITLE     Gads is a novel SH2 and SH3 domain-containing adaptor protein that
            binds to tyrosine-phosphorylated Shc
  JOURNAL   Oncogene 17 (24), 3073-3082 (1998)
   PUBMED   9872323
REFERENCE   10 (bases 1 to 5380)
  AUTHORS   Bourette,R.P., Arnaud,S., Myles,G.M., Blanchet,J.P.,
            Rohrschneider,L.R. and Mouchiroud,G.
  TITLE     Mona, a novel hematopoietic-specific adaptor interacting with the
            macrophage colony-stimulating factor receptor, is implicated in
            monocyte/macrophage development
  JOURNAL   EMBO J 17 (24), 7273-7281 (1998)
   PUBMED   9857184
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC052496.1 and AC147041.3.
            
            On Jan 22, 2014 this sequence version replaced XM_006520559.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13862062.175230.1, BC052496.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1259              BC052496.1         13-1271
            1260-5380           AC147041.3         47798-51918         c
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.88 cM"
     gene            1..5380
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="GRB2-related adaptor protein 2"
                     /db_xref="GeneID:17444"
                     /db_xref="MGI:MGI:1333842"
     exon            1..145
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            146..237
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..1128
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="monocytic adapter; monocytic adaptor; GRB-2-like
                     protein; adapter protein GRID; growth factor
                     receptor-binding protein; GRB-2-related monocytic adapter
                     protein; hematopoietic cell-associated adaptor protein
                     GrpL; Grb2-related adaptor downstream of Sch"
                     /codon_start=1
                     /product="GRB2-related adaptor protein 2"
                     /protein_id="NP_001276371.1"
                     /db_xref="CCDS:CCDS27663.1"
                     /db_xref="GeneID:17444"
                     /db_xref="MGI:MGI:1333842"
                     /translation="MEATAKFDFMASGEDELSFRTGDILKILSNQEEWLKAELGSQEG
                     YVPKNFIDIEFPEWFHEGLSRHQAENLLMGKDIGFFIIRASQSSPGDFSISVRHEDDV
                     QHFKVMRDTKGNYFLWTEKFPSLNKLVDYYRTTSISKQKQVFLRDGTQDQGHRGNSLD
                     RRSQGGPHPSGTVGEEIRPSVNRKLSDHLPLGPQQFHPHQQPSPQFTPGPQPPQQQRY
                     LQHFHQDRRGGSLDINDGHCGLGSEVNATLMHRRHTDPVQLQAAGRVRWARALYDFEA
                     LEEDELGFRSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMMR"
     exon            238..329
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            330..449
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            450..615
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            616..831
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            832..948
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     exon            949..5380
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3232..3237
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="hexamer: AATAAA"
     polyA_site      3253
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
     regulatory      4861..4866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="hexamer: AATAAA"
     regulatory      4866..4871
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="hexamer: AATACA"
     polyA_site      4882
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="major polyA site"
     polyA_site      4890
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
     regulatory      5291..5296
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
                     /note="hexamer: AACAAA"
     polyA_site      5322
                     /gene="Grap2"
                     /gene_synonym="Gads; GRAP-2; GRB2L; GRBLG; GrbX; Grf40;
                     GRID; GrpL; Mona; P38"
ORIGIN      
        1 aaaccagcac aaggcagacc tgtaggctga ggcggcgcgg taaacttgac atttgaatgc
       61 atcttcccac tggcaaaagg caattctaat aactcgttgt caaagccgag atacacgctc
      121 aactcaacct cttcgcgtcc caaagcttct cgtcaagaca tggaagccac tgccaagttt
      181 gatttcatgg cctcaggtga ggacgaactg agctttcgca ctggagacat tctgaagata
      241 ctaagcaacc aggaggagtg gctcaaggcg gagctcggaa gccaagaagg atatgtgcct
      301 aagaatttta tagatatcga gtttcctgag tggttccatg aaggcctctc tcgacatcaa
      361 gcagaaaact tactcatggg caaggacatt ggattcttca tcatcagggc cagccagagt
      421 tccccaggag acttctccat ctctgtcagg cacgaggacg acgttcagca cttcaaagtc
      481 atgcgtgaca ccaagggcaa ttacttcctg tggaccgaga agttcccatc cttaaataag
      541 ctggtggact actaccggac aacgtccatc tccaaacaga agcaggtctt ccttcgggat
      601 ggaacccaag atcagggtca ccggggcaac agcctggaca ggaggtccca gggcggccct
      661 cacccgagtg gaaccgtagg agaagaaatc cgaccttcag tgaaccggaa gctgtcagac
      721 caccttcctc tgggtcccca gcagttccac ccgcaccagc agccgtcacc ccagttcaca
      781 ccagggccgc agccccctca acagcagcgt tatctgcagc atttccacca ggaccgtcgt
      841 gggggcagcc tggacataaa cgatggacac tgcggccttg gaagtgaggt gaacgccacg
      901 ctgatgcacc ggagacacac cgaccccgta caactccagg cagcagggcg ggtgcgttgg
      961 gccagggcac tgtatgactt tgaggctctg gaagaggacg agctgggatt ccgaagcgga
     1021 gaagtggttg aagtcctgga cagctccaac ccatcttggt ggaccggccg tctgcacaac
     1081 aaactgggtc tcttccctgc caactatgtg gcccccatga tgcgatgagt cgtactgcaa
     1141 ggcttagggg cttttcgtcc aaagccgcct gcaagacaga gaggggcgag ggggtaaagc
     1201 tgaactttat aatgacacat acatgcatac atgtgcatgt gtgcccgagt acacaaaaca
     1261 tgtatatgta tgccgatggc tacacacgac attttataat agtaatttat tggcaggcag
     1321 gttggctcgt tgatcagcat gagaaggaac ttgggtggcg agggggccgt tttgccagct
     1381 cttctctgcc tgagagggca gtggggagct gggagcaggg cggggaaatg accttttcca
     1441 accctcaaac tttcaccgtt tccttcctgg cttgggaatt ttccaagaga gcaggcagct
     1501 cagcagagat cacaccccag ggatggatga acagggcaag gctgggcttg gcgaggcacg
     1561 ttaggtagtt cacctggctc tgtccactta cagggtagct cccctctttt gcatagagca
     1621 ggcgctgcgc cctgagaatt aaggtgtagc agatgcaggg cttcaggcac caggcaagtc
     1681 tgagcaggtc gccagcaagg gtgtggtcgg gaggctcctc ttggtgtggg agagacacgc
     1741 aggctcaggc ctagccgatg tctggctagg acttttactt tccaccagag actgtccctt
     1801 gggcaatcct cttcccctac ggctgacagg agagttagaa cggagcgatg ccacagaggt
     1861 tctccacagg cccttgtggc ttacttgaat gtaacttgtt tcctcggctg cagagaaagc
     1921 tgcagcctag attcttggaa gtgcttggag aagccctggc ggagaatgtg ggatgtgtca
     1981 gcagcctcac tctgtggtta caagagtgtc tctgtggagc tcaattgggt tttgctcctg
     2041 attttctgtg gttgaggaca tctttgccag tggacgaaag ggaagccaag agtgctgact
     2101 gtggatcgac tgataaattg acagactgac tctaaccctg acaggagctt tcacggaaat
     2161 gggtttcctc ttctgtttga gcgctaggcc ttcccctatc ctccctccac ctgggaagag
     2221 gaagcccact gccactgtcc ctctgtcacc ttaaactaga attgtgtgtc ctggggaatg
     2281 gagcacagag aagcctggct gggtggggag ggggactctg gagaggaagc ccagtgcttt
     2341 tgggcagcct gggagtaaag atgtcagaac agaaggtact tacaagggtg ttggtgtagt
     2401 cgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttct aagataggaa
     2461 aggaagagct gggtgaaagg ggtggtaagc atatgtgatg ggaggggggc tgtctccttg
     2521 tcccctcctg ctttagggag ggactggagg gcatctgtta gtgagggaga caaaagaaca
     2581 gtctgtgact caggagtcac tgcagtgtgg cacatactct gtcccttgtg actctgattc
     2641 aacaatcttc tgatccctag gttcccttta gagagaaaga gagggaccag gagggaagag
     2701 atcatagcct ggaggctcag ggatgcagct gcagccctgg gacaggaaag cagagacagt
     2761 gtcagaagat gggtggcagg tgggagacag ggagactgtg gggggtgggg gtggggtagt
     2821 atcaattggg gcagcaaggg gagacatggg caggtggtac agaaagaact cttacccctc
     2881 ccccggagaa ggtaggagcc ccccccctaa cacccccccc ccagcaggtg aggaaagagt
     2941 gcatcaggag gaggtgaagg gcagagaagg cgccaaggag gaaaaatgag gccggaagct
     3001 gaggcaaagt gggcccaagc ttggtagtag gtgatgtgag acattacagt aaataaaagc
     3061 caaaagtggt gtttggtttt acagtatttg caaaccatat gctttccaac agcccacaaa
     3121 cttcatccat ttggctgatt ctgtgggagg agcagccctg gctgaggcag agatgactag
     3181 cctggttctg gtctagttac tatgccttga aatactctat gagaatacag taataaagtt
     3241 ttgccagcct gtatctggtg ctcatgctgc acagccctag gagaaggggg tactcctcac
     3301 cttggggcag caaaacccct agaacaggca tcctgttcta gcaggagccc tggtgggaga
     3361 aggtaggagg aattctttat tgaggtatcc tcattagcag ggttcgattc gaaagggaac
     3421 aggagagtca ccccaaacca tgaagggaga ccaaggactc ccaggggctg agcctaggag
     3481 gccagcacca aactgtctac ctggggctca gatctctttg gattctgcta ctgcttcctg
     3541 ggaagtaggt aaggaagaga gcatgagcca tcataccccc caactctctc tctctctctc
     3601 tctggctgca cagccaactg caagtacacg gtctctgatt ttggccgaca caccataggt
     3661 gcgacttcat gacatctgag cagagaactt ctaagaagag ccagttcctg ttgaccaatc
     3721 agagtttcct ccccacttgc tgaaaaatca cttcatttcg aatatccagc agctaccatg
     3781 gcccactccc cattacatgg tctcagtctt tccaagatct caagtatgtg agcattgtct
     3841 gggggccgtg ttaaaatgct ggctctgact cagccttata tctatctcta acaagctccc
     3901 aggtgctttg atcagcaagg gcatcggtct cacaaatcta attggattct ttttctgggg
     3961 atagtcagcc actgctctct gttgctagcg aataagtcac tttggctttg ggacaggcac
     4021 aactcgttac tgtctttatg acaccattcc tgtttggcgg ccagtgactt cttttttaca
     4081 tcttcatcgg ggatcagaaa ttttccaagt gacaggcatt tggctggaga ctcacacagg
     4141 aattccctac tagaaatcac tttaacagga gcagaagctg tggaaggtgc aggaagactt
     4201 ctcaggaaat cctgaaagag aaaaatcaat gtccatcact ttgggctctg ggcaaggcag
     4261 ggaatatcac tagatctcag ctcacagccc acacaaggga cagaccgtcc tggtcaaaat
     4321 aattgcatga cacaattgtt ttgtctagtt tcaatgtgac tatctgtaag acaagagtgt
     4381 gaaaaaaatc taccactgca attctttaaa acaaatttat ttttgttatt gtttttcaag
     4441 tccttccact tcatctccca gccggcggca tctttctgtc tcccatagca actgcactat
     4501 cttctgtact tgttgccctg cattccttcc tgtgcctggt acactctcca ctcagacacc
     4561 aaggcaatct ttggacagac aaacccaatc acaccacttt tcttcacagg catttatcct
     4621 tgaacctaaa atcccagccc ggtcttgcaa ggtctggccc cttcgggatc atctctccaa
     4681 cttcctcttg ctttcatacc ccttccccac tgtccttcct tcagcctcca gatggctaag
     4741 aaccttccga agggggagtc ataactgtgg gtccccccag gatgcacatc ctgtgctttc
     4801 cagctgactg ctgttcagat ctgggttcac acgtcacttc ctctcatcac ccccatgtgg
     4861 aataaaatac atctttagat tacctcttga gtcccatggt tttcctgaac accatgcaca
     4921 cagatggggg cactagatcg agtggtgtga ccattagact gtatgcttct tggggtgtgt
     4981 gtggggtcat tgtattttgt agatacttta tatattcatg gcacatgggg aaggctgtca
     5041 gtagatatgt gtctaatgaa tgatattgtg tttctctaag gtgaaaactc aggtataaaa
     5101 tgagaaaagg gtgagacaag atgctctaac gagaaggtga aacactctta caaggaaact
     5161 cattaagata ggaagtaacc aactcaaaat accttcagga agtccctgaa actgactaga
     5221 ttaactaggc ccctttctcc tctgtaatat ataagcagca aagacagctg tgattcttag
     5281 aagattctca aacaaaacat cccagaataa tcaaagacca gccaaaagcc tgaaaaaaaa
     5341 aaaaaaaaaa aaaaaaagaa gagaccagct gaactgcctg
//
