LOCUS       NM_026240               2611 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus GRAM domain containing 2B (Gramd2b), transcript
            variant 2, mRNA.
ACCESSION   NM_026240 XM_993179
VERSION     NM_026240.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY112662.1, AK164050.1 and BE198493.1.
            
            On Nov 25, 2006 this sequence version replaced NM_026240.1.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' UTR, lacks a portion of the 5' coding region, and initiates
            translation at an alternate start codon, compared to variant 1. The
            encoded isoform (2) has a distinct N-terminus and is shorter than
            isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.114555.1, AK164050.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-313               BY112662.1         2-314
            314-2377            AK164050.1         299-2362
            2378-2611           BE198493.1         1-234               c
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 30.43 cM"
     gene            1..2611
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="GRAM domain containing 2B"
                     /db_xref="GeneID:107022"
                     /db_xref="MGI:MGI:1914815"
     exon            1..226
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1442
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="isoform 2 is encoded by transcript variant 2; HCV
                     NS3-transactivated protein 2; GRAM domain-containing
                     protein 3; GRAM domain-containing protein 2B; GRAM domain
                     containing 3"
                     /codon_start=1
                     /product="GRAM domain-containing protein 2B isoform 2"
                     /protein_id="NP_080516.2"
                     /db_xref="CCDS:CCDS50290.1"
                     /db_xref="GeneID:107022"
                     /db_xref="MGI:MGI:1914815"
                     /translation="MTELQQEVEDAKPAKVLTKREGKVGPAHSEAENGAEDRRRVSKS
                     PTAQSPTSSVEAESPDQKRSLGLWSKSSFDGSSLLSDKNDCKTESKTDSKTERKKSSS
                     SSQYKANMHFHKLFLDVPTEEPLRQSFTCALQKEILYQGKLFVSENWICFHSKVFGKD
                     TKISIPAFSVTLIKKTKTALLVPNALIIATVTDRYIFVSLLSRDSTYKLIKSVCGHLE
                     NTSVGNSPNPSSAENSFRADRPSSLPLDFNDEFSDLDGVVRQRRQDLEGYSSSGSQTP
                     ESENSRDFHVTESQTVLNVTKGETKPPRTDAHGSRAPDGKAKILPAHGQSETIGILHK
                     MESRKCPTLHHILIVYAIIVCALIISTFYMRYRINTLEERLGTLTSIMDPHSTEQTAP
                     SGLGSQMQLNVEVLCQELTANIVKLEKIQNNLQKLLENGD"
     exon            227..346
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            347..458
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..525
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..629
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            630..725
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..797
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..878
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            879..990
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1113
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1201
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1306
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1400
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..2605
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2354..2359
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="hexamer: AATAAG"
     polyA_site      2378
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="major polyA site"
     regulatory      2575..2580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="hexamer: AATAAA"
     polyA_site      2603
                     /gene="Gramd2b"
                     /gene_synonym="9030613F08Rik; 9130427A09Rik; Gramd3;
                     NS3TP2"
                     /note="major polyA site"
ORIGIN      
        1 aatcgcgccg gctccaacgt gtccatgttg gcggcccggg agcaccgccg cctggatgcg
       61 cagcgctagg gtgccggaga gctgggagcc gggagccccg aatagggagc cgagtgctgg
      121 ggtacgcccg ggacgcggcc ccgatgaccg agctccagca agaggtggag gacgccaagc
      181 cagccaaagt gctcaccaag agagaaggca aagttggccc ggcccactca gaggctgaga
      241 atggtgcgga ggacagaagg agggtcagca agtcgccaac agcccaatct cctacctcat
      301 ctgtggaggc cgagtccccg gaccagaaga ggagccttgg cctgtggtca aaatccagtt
      361 ttgatggttc cagtttgttg agtgataaga atgactgtaa aactgaaagc aagactgact
      421 ctaagaccga aagaaaaaag tcttcatcgt ctagtcagta caaggcgaac atgcactttc
      481 acaagttatt cctggatgtc ccaactgagg agccactgag gcaaagcttt acctgtgctc
      541 ttcagaaaga aattctgtac caaggaaagc tctttgtgtc ggaaaactgg atttgttttc
      601 attccaaggt ctttggaaaa gacactaaga tctccattcc agctttctcg gtaaccttga
      661 tcaagaaaac caaaactgcc cttctggtgc caaacgccct gatcatcgcg acggtcacag
      721 acaggtacat attcgtttcc ttgctgtcca gagactcaac ctacaaactg ataaaatctg
      781 tgtgcggaca cttagagaac acaagtgttg gtaacagtcc caacccatct tctgctgaaa
      841 acagtttccg ggcagatcgc ccttcgtctc tgcctctgga tttcaatgat gaattctcag
      901 acctggacgg agtggttcga caacgaaggc aagacttgga aggatacagc agctcaggat
      961 cccagactcc ggaatctgag aactcccgag acttccacgt gaccgaatct cagacagttc
     1021 tgaatgtcac aaagggagaa acaaaaccac ctcggacaga tgcccacggg agcagagcac
     1081 ctgatggaaa agccaagatc ctgcctgccc atggtcaatc agaaactatt ggaattttac
     1141 ataaaatgga gtctcggaag tgcccgactc tccatcacat cctgatagtc tatgcaatta
     1201 ttgtctgtgc cctgatcatc tccaccttct acatgagata caggattaac actctggagg
     1261 agcgcctggg gaccctgacg tccatcatgg atccccacag tactgagcag acagccccat
     1321 ccggcctggg atcacaaatg cagttaaatg tggaggtcct ctgccaagaa cttacagcta
     1381 acatagtgaa attggaaaag atacaaaaca acttacaaaa gctgcttgag aatggtgact
     1441 gagtgaccag ctgctgggac caacataaat acctcacgtt ctcccttgaa gacggtgctt
     1501 cttgagagtc tgttgtgcgc cccctgctgg ccagaggcgc gagacgctga gaatccagat
     1561 gtgtttgccc tttggaggtc tccagcttag gaaagggggt ggggaaaagg gaatgagttt
     1621 gcttttgtgc aataaaagtt ggccttgtct ctgctgctgc tgcctgaaga taacagaccc
     1681 atctcgggag gtgagcaagg tcccggaaga cacctgtagc acgagggtta tggtaatgat
     1741 caggaaacac agcactccat gcttttcagc cctttcttag ccttctcttt catgccttca
     1801 cccatcctgg tcagccatca gacctaaggg gtacactgtt taccagcaaa ctcaaaatgg
     1861 tcctttagag aattaaccac aaacaaggca agagccttca acaaggtggt gaatatctcc
     1921 tcctgaataa gcttgcttcc aacccctgta tttttgtggg tttttatttt cccctttcta
     1981 ctcttatgtt ctgatttctt tcatctatgc cttttaattt cccaattcag ttttgtgagc
     2041 ctgcccggta aagtgaatta agcatcccat ctgcgttgtg ctgtcttgtt cccgtgacga
     2101 gtacttaatt gaagtgattc tcctcagcct cccgtcagga atttttgatc acattcagtt
     2161 taaatcacat tcactctgaa accaaaacta atctttcagc caaaagttgt cataatgatt
     2221 taacatggga cggaaagccc cgtttctagg atcgtaagcg gacgacagaa gcagcgttga
     2281 agcaggatgt ctgtgttgga ctctaaaacc catctcagca tggtaattgg atgtctttct
     2341 cctacaataa agcaataagt ggtggtctgc taactttact tgcagtatgg gttggctggt
     2401 ttttattagt aattatttag ggtttgtaag tataaaacat tctatctgca gtgtgtctta
     2461 taaatcattt ctatgaaatg tgttttgttt aatcagataa gctaataagt ggattggtga
     2521 tgtcgtgtgt atttgcgagc atactggata actgtacctg gtgcaccagc tattaataaa
     2581 tcctcattgc ctaaggcacc ctagcaaaaa a
//
