LOCUS       NM_008162                882 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus glutathione peroxidase 4 (Gpx4), transcript variant 1,
            mRNA.
ACCESSION   NM_008162 XM_889796
VERSION     NM_008162.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Ito,J., Nakamura,T., Toyama,T., Chen,D., Berndt,C., Poschmann,G.,
            Mourao,A.S.D., Doll,S., Suzuki,M., Zhang,W., Zheng,J., Trumbach,D.,
            Yamada,N., Ono,K., Yazaki,M., Kawai,Y., Arisawa,M., Ohsaki,Y.,
            Shirakawa,H., Wahida,A., Proneth,B., Saito,Y., Nakagawa,K.,
            Mishima,E. and Conrad,M.
  TITLE     PRDX6 dictates ferroptosis sensitivity by directing cellular
            selenium utilization
  JOURNAL   Mol Cell 84 (23), 4629-4644 (2024)
   PUBMED   39547222
REFERENCE   2  (bases 1 to 882)
  AUTHORS   Fang,Y.Q., Zhang,H.K., Wei,Q.Q. and Li,Y.H.
  TITLE     Brown adipose tissue-derived exosomes improve polycystic ovary
            syndrome in mice via STAT3/GPX4 signaling pathway
  JOURNAL   FASEB J 38 (18), e70062 (2024)
   PUBMED   39305125
  REMARK    GeneRIF: Brown adipose tissue-derived exosomes improve polycystic
            ovary syndrome in mice via STAT3/GPX4 signaling pathway.
REFERENCE   3  (bases 1 to 882)
  AUTHORS   Li,X., He,J., Gao,X., Zheng,G., Chen,C., Chen,Y., Xing,Z., Wang,T.,
            Tang,J., Guo,Y. and He,Y.
  TITLE     GPX4 restricts ferroptosis of NKp46+ILC3s to control intestinal
            inflammation
  JOURNAL   Cell Death Dis 15 (9), 687 (2024)
   PUBMED   39300068
  REMARK    GeneRIF: GPX4 restricts ferroptosis of NKp46[+]ILC3s to control
            intestinal inflammation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 882)
  AUTHORS   Liu,D., Ren,L. and Liu,J.
  TITLE     METTL14 promotes chondrocyte ferroptosis in osteoarthritis via m6A
            modification of GPX4
  JOURNAL   Int J Rheum Dis 27 (8), e15297 (2024)
   PUBMED   39175261
  REMARK    GeneRIF: METTL14 promotes chondrocyte ferroptosis in osteoarthritis
            via m6A modification of GPX4.
REFERENCE   5  (bases 1 to 882)
  AUTHORS   Schneider,M., Forster,H., Boersma,A., Seiler,A., Wehnes,H.,
            Sinowatz,F., Neumuller,C., Deutsch,M.J., Walch,A., Hrabe de
            Angelis,M., Wurst,W., Ursini,F., Roveri,A., Maleszewski,M.,
            Maiorino,M. and Conrad,M.
  TITLE     Mitochondrial glutathione peroxidase 4 disruption causes male
            infertility
  JOURNAL   FASEB J 23 (9), 3233-3242 (2009)
   PUBMED   19417079
  REMARK    GeneRIF: mitochondrial GPx4 confers the vital role of selenium in
            mammalian male fertility
REFERENCE   6  (bases 1 to 882)
  AUTHORS   Pfeifer,H., Conrad,M., Roethlein,D., Kyriakopoulos,A.,
            Brielmeier,M., Bornkamm,G.W. and Behne,D.
  TITLE     Identification of a specific sperm nuclei selenoenzyme necessary
            for protamine thiol cross-linking during sperm maturation
  JOURNAL   FASEB J 15 (7), 1236-1238 (2001)
   PUBMED   11344099
REFERENCE   7  (bases 1 to 882)
  AUTHORS   Borchert,A., Schnurr,K., Thiele,B.J. and Kuhn,H.
  TITLE     Cloning of the mouse phospholipid hydroperoxide glutathione
            peroxidase gene
  JOURNAL   FEBS Lett 446 (2-3), 223-227 (1999)
   PUBMED   10100845
REFERENCE   8  (bases 1 to 882)
  AUTHORS   Gladyshev,V.N., Factor,V.M., Housseau,F. and Hatfield,D.L.
  TITLE     Contrasting patterns of regulation of the antioxidant
            selenoproteins, thioredoxin reductase, and glutathione peroxidase,
            in cancer cells
  JOURNAL   Biochem Biophys Res Commun 251 (2), 488-493 (1998)
   PUBMED   9792801
REFERENCE   9  (bases 1 to 882)
  AUTHORS   Nam,S., Nakamuta,N., Kurohmaru,M. and Hayashi,Y.
  TITLE     Cloning and sequencing of the mouse cDNA encoding a phospholipid
            hydroperoxide glutathione peroxidase
  JOURNAL   Gene 198 (1-2), 245-249 (1997)
   PUBMED   9370288
REFERENCE   10 (bases 1 to 882)
  AUTHORS   Nishiguchi,S., Joh,T., Horie,K., Zou,Z., Yasunaga,T. and Shimada,K.
  TITLE     A survey of genes expressed in undifferentiated mouse embryonal
            carcinoma F9 cells: characterization of low-abundance mRNAs
  JOURNAL   J Biochem 116 (1), 128-139 (1994)
   PUBMED   7798170
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY238083.1, AK006441.1 and
            CX733872.1.
            
            On Jan 6, 2019 this sequence version replaced NM_008162.3.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of hydrogen peroxide, organic hydroperoxides and lipid
            hydroperoxides, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            has a high preference for lipid hydroperoxides and protects cells
            against membrane lipid peroxidation and cell death. It is also
            required for normal sperm development; thus, it has been identified
            as a 'moonlighting' protein because of its ability to serve dual
            functions as a peroxidase, as well as a structural protein in
            mature spermatozoa. Disruption of this gene in mouse spermatocytes
            is associated with male infertility. This isozyme is also a
            selenoprotein, containing the rare amino acid selenocysteine (Sec)
            at its active site. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal.
            Transcript variants resulting from alternative splicing or use of
            alternate promoters have been described to encode isoforms with
            different subcellular localization. Pseudogenes of this locus have
            been identified on chromosomes 10 and 17. [provided by RefSeq, Jan
            2019].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the canonical isoform A (also known as
            phGPx). A similar isoform in rat(L-form) has been shown to be
            localized to the mitochondria (PMID:9988735).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK168027.1, D87896.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 19417079; reported by
                                                  MitoCarta
            multifunctional gene product(s)    :: inferred from conservation
            protein contains selenocysteine    :: PMID: 9370288
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BY238083.1         2-32
            32-880              AK006441.1         4-852
            881-882             CX733872.1         401-402
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..882
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /note="glutathione peroxidase 4"
                     /db_xref="GeneID:625249"
                     /db_xref="MGI:MGI:104767"
     exon            1..151
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..661
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /EC_number="1.11.1.9"
                     /EC_number="1.11.1.12"
                     /note="UGA stop codon recoded as selenocysteine; isoform A
                     precursor is encoded by transcript variant 1; phospholipid
                     hydroperoxide glutathione peroxidase, nuclear;
                     phospholipid hydroperoxide glutathione peroxidase,
                     mitochondrial; sperm nuclei glutathione peroxidase;
                     phospholipid hydroperoxide glutathione peroxidase GPX4"
                     /codon_start=1
                     /transl_except=(pos:284..286,aa:Sec)
                     /product="phospholipid hydroperoxide glutathione
                     peroxidase GPX4 isoform A precursor"
                     /protein_id="NP_032188.3"
                     /db_xref="CCDS:CCDS35973.1"
                     /db_xref="GeneID:625249"
                     /db_xref="MGI:MGI:104767"
                     /translation="MSWGRLSRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEF
                     SAKDIDGHMVCLDKYRGFVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPC
                     NQFGRQEPGSNQEIKEFAAGYNVKFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNA
                     IKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL"
     exon            152..246
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     exon            247..391
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     exon            392..543
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     exon            544..568
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     exon            569..628
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     exon            629..882
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      707..804
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      864..869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpx4"
                     /gene_synonym="GPx-4; GSHPx-4; mtPHGPx; PHGPx; snGPx"
ORIGIN      
        1 atactcggcc tcgcgcgtcc attggtcggc tgcgtgaggg gaggagccgc tggctccggc
       61 cgccgagatg agctggggcc gtctgagccg cttacttaag ccagcactgc tgtgcggggc
      121 tctggctgcg cctggtctgg caggcaccat gtgtgcatcc cgcgatgatt ggcgctgtgc
      181 gcgctccatg cacgaattct cagccaagga catcgacggg cacatggtct gcctggataa
      241 gtacaggggt ttcgtgtgca tcgtcaccaa cgtggcctcg caatgaggca aaactgacgt
      301 aaactacact cagctagtcg atctgcatgc ccgatatgct gagtgtggtt tacgaatcct
      361 ggccttcccc tgcaaccagt ttgggaggca ggagccagga agtaatcaag aaatcaagga
      421 gtttgcagcc ggctacaacg tcaagtttga catgtacagc aagatctgtg taaatgggga
      481 cgatgcccac ccactgtgga aatggatgaa agtccagccc aagggcaggg gcatgctggg
      541 aaatgccatc aaatggaact ttaccaagtt tctcattgat aagaacggct gcgtggtgaa
      601 gcgctatggt cccatggagg agccccaggt gatagagaag gacctgccgt gctatctcta
      661 gctagcccta caagtgtgtg cccctacacc gagcccccct gccctgtgac ccctggagcc
      721 ttccaccccg gcactcatga aggtctgcct gaaaaccagc ctgctggtgg ggcagtcctg
      781 aggacctggc gtgcatccct gccggaggaa ggtccagagg cctgtggccc tgggctcgag
      841 cttcaccctg gctgccttgt gggaataaaa tgtagaaatg tg
//
