LOCUS       NM_008161               1541 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus glutathione peroxidase 3 (Gpx3), transcript variant 1,
            mRNA.
ACCESSION   NM_008161
VERSION     NM_008161.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Covington,T.A., Pilz,P.M., Mulhern,R.M., Ngoy,S., Loscalzo,A.,
            Liu,J., Fisch,S. and Grune,J.
  TITLE     GPx3 deficiency exacerbates maladaptive right ventricular
            remodeling in experimental pulmonary artery banding
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 324 (4), L550-L556 (2023)
   PUBMED   36880685
  REMARK    GeneRIF: GPx3 deficiency exacerbates maladaptive right ventricular
            remodeling in experimental pulmonary artery banding.
REFERENCE   3  (bases 1 to 1541)
  AUTHORS   Wu,X., Tang,S., Dai,Q., Yi,B., Yang,S., Sun,J., Zhong,Y., Lin,W.,
            Liu,J., Liu,Y., Wang,J., Liu,J., Liao,Q., Zhang,W. and Zhang,H.
  TITLE     Vitamin D-vitamin D receptor alleviates oxidative stress in
            ischemic acute kidney injury via upregulating glutathione
            peroxidase 3
  JOURNAL   FASEB J 37 (2), e22738 (2023)
   PUBMED   36583727
  REMARK    GeneRIF: Vitamin D-vitamin D receptor alleviates oxidative stress
            in ischemic acute kidney injury via upregulating glutathione
            peroxidase 3.
REFERENCE   4  (bases 1 to 1541)
  AUTHORS   Li,G., Qin,Y., Cheng,Z., Cheng,X., Wang,R., Luo,X., Zhao,Y.,
            Zhang,D. and Li,G.
  TITLE     Gpx3 and Egr1 Are Involved in Regulating the Differentiation Fate
            of Cardiac Fibroblasts under Pressure Overload
  JOURNAL   Oxid Med Cell Longev 2022, 3235250 (2022)
   PUBMED   35799890
  REMARK    GeneRIF: Gpx3 and Egr1 Are Involved in Regulating the
            Differentiation Fate of Cardiac Fibroblasts under Pressure
            Overload.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1541)
  AUTHORS   Tham,D.M., Whitin,J.C., Kim,K.K., Zhu,S.X. and Cohen,H.J.
  TITLE     Expression of extracellular glutathione peroxidase in human and
            mouse gastrointestinal tract
  JOURNAL   Am J Physiol 275 (6), G1463-G1471 (1998)
   PUBMED   9843785
REFERENCE   6  (bases 1 to 1541)
  AUTHORS   Kingsley,P.D., Whitin,J.C., Cohen,H.J. and Palis,J.
  TITLE     Developmental expression of extracellular glutathione peroxidase
            suggests antioxidant roles in deciduum, visceral yolk sac, and skin
  JOURNAL   Mol Reprod Dev 49 (4), 343-355 (1998)
   PUBMED   9508085
REFERENCE   7  (bases 1 to 1541)
  AUTHORS   Schwaab,V., Baud,E., Ghyselinck,N., Mattei,M.G., Dufaure,J.P. and
            Drevet,J.R.
  TITLE     Cloning of the mouse gene encoding plasma glutathione peroxidase:
            organization, sequence and chromosomal localization
  JOURNAL   Gene 167 (1-2), 25-31 (1995)
   PUBMED   8566787
REFERENCE   8  (bases 1 to 1541)
  AUTHORS   Mirochnitchenko,O., Palnitkar,U., Philbert,M. and Inouye,M.
  TITLE     Thermosensitive phenotype of transgenic mice overproducing human
            glutathione peroxidases
  JOURNAL   Proc Natl Acad Sci U S A 92 (18), 8120-8124 (1995)
   PUBMED   7667255
REFERENCE   9  (bases 1 to 1541)
  AUTHORS   Maser,R.L., Magenheimer,B.S. and Calvet,J.P.
  TITLE     Mouse plasma glutathione peroxidase. cDNA sequence analysis and
            renal proximal tubular expression and secretion
  JOURNAL   J Biol Chem 269 (43), 27066-27073 (1994)
   PUBMED   7929449
REFERENCE   10 (bases 1 to 1541)
  AUTHORS   Chu,F.F., Esworthy,R.S., Doroshow,J.H., Doan,K. and Liu,X.F.
  TITLE     Expression of plasma glutathione peroxidase in human liver in
            addition to kidney, heart, lung, and breast in humans and rodents
  JOURNAL   Blood 79 (12), 3233-3238 (1992)
   PUBMED   1339300
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY061328.1, AK004942.1 and
            BG796554.1.
            
            On Aug 3, 2016 this sequence version replaced NM_008161.3.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by
            glutathione, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            is secreted and is highly expressed in mouse kidney, which appears
            to be the major source of the enzyme in plasma. It has a role in
            mouse organogenesis, and dysregulation of this isozyme has been
            associated with obesity-related metabolic complications,
            platelet-dependent thrombosis, colitis-associated carcinoma, and
            thermosensitive phenotype. This isozyme is also a selenoprotein,
            containing the rare amino acid selenocysteine (Sec) at its active
            site. Sec is encoded by the UGA codon, which normally signals
            translation termination. The 3' UTRs of selenoprotein mRNAs contain
            a conserved stem-loop structure, designated the Sec insertion
            sequence (SECIS) element, that is necessary for the recognition of
            UGA as a Sec codon, rather than as a stop signal. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Aug 2016].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037134.1, AK002219.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            protein contains selenocysteine  :: PMID: 7929449
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BY061328.1         2-73
            73-1508             AK004942.1         2-1437
            1509-1541           BG796554.1         31-63               c
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 32.13 cM"
     gene            1..1541
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="glutathione peroxidase 3"
                     /db_xref="GeneID:14778"
                     /db_xref="MGI:MGI:105102"
     exon            1..199
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..793
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /EC_number="1.11.1.9"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     precursor is encoded by transcript variant 1; plasma
                     glutathione peroxidase; GPx-3; GPx-P; plasma GPx;
                     extracellular GPx"
                     /codon_start=1
                     /transl_except=(pos:329..331,aa:Sec)
                     /product="glutathione peroxidase 3 isoform 1 precursor"
                     /protein_id="NP_032187.2"
                     /db_xref="CCDS:CCDS24703.1"
                     /db_xref="GeneID:14778"
                     /db_xref="MGI:MGI:105102"
                     /translation="MARILRASCLLSLLLAGFVPPGRGQEKSKTDCHGGMSGTIYEYG
                     ALTIDGEEYIPFKQYAGKYILFVNVASYUGLTDQYLELNALQEELGPFGLVILGFPSN
                     QFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTA
                     ELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDILSYMRRQ
                     AALSARGK"
     exon            200..353
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            354..471
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            472..571
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            572..1522
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1307..1380
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1492..1497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="hexamer: AGTAAA"
     polyA_site      1512
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="major polyA site"
ORIGIN      
        1 ggaagccccc gcctggctgc ggattggcca cagtggatgg cttgaaatgt ggctgagcgc
       61 ttcggacacc tcagacggac ggtggccacc ccaagacgcg ccccagcccg ccatggcccg
      121 gatcctccgg gcatcctgcc ttctgtccct gctcctggcc gggtttgttc cgccgggccg
      181 gggacaagag aagtctaaga cagactgcca tggcggtatg agtggtacca tctacgagta
      241 tggagccctc accatcgatg gggaggaata cattcctttt aagcagtatg caggcaaata
      301 tatcctcttt gtcaacgtag ccagctactg aggtctgaca gaccaatacc ttgaactgaa
      361 tgcactacaa gaagaacttg ggccatttgg cttggtcatt ctgggcttcc cttccaacca
      421 atttggcaaa caggagccag gcgagaactc ggagatactc cccagtctca agtatgttcg
      481 accaggtggg ggctttgtgc ctaatttcca gctctttgag aaaggagatg tgaacgggga
      541 gaaagagcag aaattctaca ctttcctgaa gaactcctgc cctcccactg cagaactcct
      601 gggctcacct ggccgcctct tttgggaacc catgaagatc catgacatcc gctggaactt
      661 tgagaagttc ctggtggggc cagatggcat accggttatg cgctggtacc accggaccac
      721 agtcagcaac gtcaagatgg acatcctgtc ttacatgagg cggcaggcag ccctgagcgc
      781 cagggggaag taactgatgc ccccacccta cccctacccc ctgcccatca tgcaagggcc
      841 gaggaggggc tcttcaggaa ggaagccaca ttcccagtca ttctaccccc accccagatt
      901 ctctttctta ttacataaaa gacaagcctg gcacaactgt gtgtctgaac cactgtggac
      961 acgtgacaat tgtcccagtg tgtgcatggc tacacagcca cgtatctgcc tgcttgaaac
     1021 ccagggatgg tccatctgtg tttacggctt ggcacaacac cctcatattt ttttcagctt
     1081 tctgttccaa atgagcccaa aggaaacaca agttctaggt ccaatggttc tgctcaaacc
     1141 tgaacatcat tcttggggcc agcatctccc acatgcccac actacacacc accagcctcc
     1201 ttcttccttc ctgaaggacc ctcctgagcc cccaagccca tcccacagtg ctcctgagac
     1261 cagccaagac aactgtgagc gcgatggccg tgtaccccag gtcaggggtg gtgtctctat
     1321 gaaggagggg cccgaagccc ttgtgggcgg gcctcccctg agcccgtctg tggtgccagc
     1381 ccttagtgca ttcaggctta ggctcccagg cagggacact acccccgcgc ctctggagga
     1441 catgctatcc tctcactctg tccactggta tctcaacacc cccatctgcc cagtaaaggt
     1501 ctttctgcag cagttcagcc taaaaaaaaa aaaaaaaaaa a
//
