LOCUS       NM_001329860            1637 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus glutathione peroxidase 3 (Gpx3), transcript variant 2,
            mRNA.
ACCESSION   NM_001329860
VERSION     NM_001329860.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Covington,T.A., Pilz,P.M., Mulhern,R.M., Ngoy,S., Loscalzo,A.,
            Liu,J., Fisch,S. and Grune,J.
  TITLE     GPx3 deficiency exacerbates maladaptive right ventricular
            remodeling in experimental pulmonary artery banding
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 324 (4), L550-L556 (2023)
   PUBMED   36880685
  REMARK    GeneRIF: GPx3 deficiency exacerbates maladaptive right ventricular
            remodeling in experimental pulmonary artery banding.
REFERENCE   3  (bases 1 to 1637)
  AUTHORS   Wu,X., Tang,S., Dai,Q., Yi,B., Yang,S., Sun,J., Zhong,Y., Lin,W.,
            Liu,J., Liu,Y., Wang,J., Liu,J., Liao,Q., Zhang,W. and Zhang,H.
  TITLE     Vitamin D-vitamin D receptor alleviates oxidative stress in
            ischemic acute kidney injury via upregulating glutathione
            peroxidase 3
  JOURNAL   FASEB J 37 (2), e22738 (2023)
   PUBMED   36583727
  REMARK    GeneRIF: Vitamin D-vitamin D receptor alleviates oxidative stress
            in ischemic acute kidney injury via upregulating glutathione
            peroxidase 3.
REFERENCE   4  (bases 1 to 1637)
  AUTHORS   Li,G., Qin,Y., Cheng,Z., Cheng,X., Wang,R., Luo,X., Zhao,Y.,
            Zhang,D. and Li,G.
  TITLE     Gpx3 and Egr1 Are Involved in Regulating the Differentiation Fate
            of Cardiac Fibroblasts under Pressure Overload
  JOURNAL   Oxid Med Cell Longev 2022, 3235250 (2022)
   PUBMED   35799890
  REMARK    GeneRIF: Gpx3 and Egr1 Are Involved in Regulating the
            Differentiation Fate of Cardiac Fibroblasts under Pressure
            Overload.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1637)
  AUTHORS   Tham,D.M., Whitin,J.C., Kim,K.K., Zhu,S.X. and Cohen,H.J.
  TITLE     Expression of extracellular glutathione peroxidase in human and
            mouse gastrointestinal tract
  JOURNAL   Am J Physiol 275 (6), G1463-G1471 (1998)
   PUBMED   9843785
REFERENCE   6  (bases 1 to 1637)
  AUTHORS   Kingsley,P.D., Whitin,J.C., Cohen,H.J. and Palis,J.
  TITLE     Developmental expression of extracellular glutathione peroxidase
            suggests antioxidant roles in deciduum, visceral yolk sac, and skin
  JOURNAL   Mol Reprod Dev 49 (4), 343-355 (1998)
   PUBMED   9508085
REFERENCE   7  (bases 1 to 1637)
  AUTHORS   Schwaab,V., Baud,E., Ghyselinck,N., Mattei,M.G., Dufaure,J.P. and
            Drevet,J.R.
  TITLE     Cloning of the mouse gene encoding plasma glutathione peroxidase:
            organization, sequence and chromosomal localization
  JOURNAL   Gene 167 (1-2), 25-31 (1995)
   PUBMED   8566787
REFERENCE   8  (bases 1 to 1637)
  AUTHORS   Mirochnitchenko,O., Palnitkar,U., Philbert,M. and Inouye,M.
  TITLE     Thermosensitive phenotype of transgenic mice overproducing human
            glutathione peroxidases
  JOURNAL   Proc Natl Acad Sci U S A 92 (18), 8120-8124 (1995)
   PUBMED   7667255
REFERENCE   9  (bases 1 to 1637)
  AUTHORS   Maser,R.L., Magenheimer,B.S. and Calvet,J.P.
  TITLE     Mouse plasma glutathione peroxidase. cDNA sequence analysis and
            renal proximal tubular expression and secretion
  JOURNAL   J Biol Chem 269 (43), 27066-27073 (1994)
   PUBMED   7929449
REFERENCE   10 (bases 1 to 1637)
  AUTHORS   Chu,F.F., Esworthy,R.S., Doroshow,J.H., Doan,K. and Liu,X.F.
  TITLE     Expression of plasma glutathione peroxidase in human liver in
            addition to kidney, heart, lung, and breast in humans and rodents
  JOURNAL   Blood 79 (12), 3233-3238 (1992)
   PUBMED   1339300
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY061328.1, AK004942.1,
            AW319411.1 and BG796554.1.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by
            glutathione, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            is secreted and is highly expressed in mouse kidney, which appears
            to be the major source of the enzyme in plasma. It has a role in
            mouse organogenesis, and dysregulation of this isozyme has been
            associated with obesity-related metabolic complications,
            platelet-dependent thrombosis, colitis-associated carcinoma, and
            thermosensitive phenotype. This isozyme is also a selenoprotein,
            containing the rare amino acid selenocysteine (Sec) at its active
            site. Sec is encoded by the UGA codon, which normally signals
            translation termination. The 3' UTRs of selenoprotein mRNAs contain
            a conserved stem-loop structure, designated the Sec insertion
            sequence (SECIS) element, that is necessary for the recognition of
            UGA as a Sec codon, rather than as a stop signal. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Aug 2016].
            
            Transcript Variant: This variant (2) has an additional in-frame
            coding exon compared to variant 1. The resulting isoform (2) is
            longer, containing a 32 aa protein segment not found in isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AW319411.1, SRR5189676.113951.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            protein contains selenocysteine  :: PMID: 7929449
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                BY061328.1         2-73
            73-159              AK004942.1         2-88
            160-647             AW319411.1         1-488
            648-1604            AK004942.1         481-1437
            1605-1637           BG796554.1         31-63               c
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 32.13 cM"
     gene            1..1637
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="glutathione peroxidase 3"
                     /db_xref="GeneID:14778"
                     /db_xref="MGI:MGI:105102"
     exon            1..199
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..889
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /EC_number="1.11.1.9"
                     /note="UGA stop codon recoded as selenocysteine; isoform 2
                     precursor is encoded by transcript variant 2; plasma
                     glutathione peroxidase; GPx-3; GPx-P; plasma GPx;
                     extracellular GPx"
                     /codon_start=1
                     /transl_except=(pos:329..331,aa:Sec)
                     /product="glutathione peroxidase 3 isoform 2 precursor"
                     /protein_id="NP_001316789.1"
                     /db_xref="GeneID:14778"
                     /db_xref="MGI:MGI:105102"
                     /translation="MARILRASCLLSLLLAGFVPPGRGQEKSKTDCHGGMSGTIYEYG
                     ALTIDGEEYIPFKQYAGKYILFVNVASYUGLTDQYLGFLGSPGCPQTCSVDQDGLKLR
                     SACLLNAEIKELNALQEELGPFGLVILGFPSNQFGKQEPGENSEILPSLKYVRPGGGF
                     VPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPMKIHDIRWNFEKF
                     LVGPDGIPVMRWYHRTTVSNVKMDILSYMRRQAALSARGK"
     exon            200..353
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            354..449
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            450..567
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            568..667
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     exon            668..1618
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1403..1476
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1588..1593
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="hexamer: AGTAAA"
     polyA_site      1608
                     /gene="Gpx3"
                     /gene_synonym="EGPx; GPx; GSHPx-3; GSHPx-P"
                     /note="major polyA site"
ORIGIN      
        1 ggaagccccc gcctggctgc ggattggcca cagtggatgg cttgaaatgt ggctgagcgc
       61 ttcggacacc tcagacggac ggtggccacc ccaagacgcg ccccagcccg ccatggcccg
      121 gatcctccgg gcatcctgcc ttctgtccct gctcctggcc gggtttgttc cgccgggccg
      181 gggacaagag aagtctaaga cagactgcca tggcggtatg agtggtacca tctacgagta
      241 tggagccctc accatcgatg gggaggaata cattcctttt aagcagtatg caggcaaata
      301 tatcctcttt gtcaacgtag ccagctactg aggtctgaca gaccaatacc ttgggtttct
      361 gggtagccct ggctgtcctc agacttgctc tgtggaccaa gatggcctca aactcaggtc
      421 tgcttgcctc ctaaatgctg agatcaaaga actgaatgca ctacaagaag aacttgggcc
      481 atttggcttg gtcattctgg gcttcccttc caaccaattt ggcaaacagg agccaggcga
      541 gaactcggag atactcccca gtctcaagta tgttcgacca ggtgggggct ttgtgcctaa
      601 tttccagctc tttgagaaag gagatgtgaa cggggagaaa gagcagaaat tctacacttt
      661 cctgaagaac tcctgccctc ccactgcaga actcctgggc tcacctggcc gcctcttttg
      721 ggaacccatg aagatccatg acatccgctg gaactttgag aagttcctgg tggggccaga
      781 tggcataccg gttatgcgct ggtaccaccg gaccacagtc agcaacgtca agatggacat
      841 cctgtcttac atgaggcggc aggcagccct gagcgccagg gggaagtaac tgatgccccc
      901 accctacccc taccccctgc ccatcatgca agggccgagg aggggctctt caggaaggaa
      961 gccacattcc cagtcattct acccccaccc cagattctct ttcttattac ataaaagaca
     1021 agcctggcac aactgtgtgt ctgaaccact gtggacacgt gacaattgtc ccagtgtgtg
     1081 catggctaca cagccacgta tctgcctgct tgaaacccag ggatggtcca tctgtgttta
     1141 cggcttggca caacaccctc atattttttt cagctttctg ttccaaatga gcccaaagga
     1201 aacacaagtt ctaggtccaa tggttctgct caaacctgaa catcattctt ggggccagca
     1261 tctcccacat gcccacacta cacaccacca gcctccttct tccttcctga aggaccctcc
     1321 tgagccccca agcccatccc acagtgctcc tgagaccagc caagacaact gtgagcgcga
     1381 tggccgtgta ccccaggtca ggggtggtgt ctctatgaag gaggggcccg aagcccttgt
     1441 gggcgggcct cccctgagcc cgtctgtggt gccagccctt agtgcattca ggcttaggct
     1501 cccaggcagg gacactaccc ccgcgcctct ggaggacatg ctatcctctc actctgtcca
     1561 ctggtatctc aacaccccca tctgcccagt aaaggtcttt ctgcagcagt tcagcctaaa
     1621 aaaaaaaaaa aaaaaaa
//
