LOCUS       NM_030677               1071 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus glutathione peroxidase 2 (Gpx2), mRNA.
ACCESSION   NM_030677
VERSION     NM_030677.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ahmed,K.M., Veeramachaneni,R., Deng,D., Putluri,N., Putluri,V.,
            Cardenas,M.F., Wheeler,D.A., Decker,W.K., Frederick,A.I., Kazi,S.,
            Sikora,A.G., Sandulache,V.C. and Frederick,M.J.
  TITLE     Glutathione peroxidase 2 is a metabolic driver of the tumor immune
            microenvironment and immune checkpoint inhibitor response
  JOURNAL   J Immunother Cancer 10 (8) (2022)
   PUBMED   36002187
  REMARK    GeneRIF: Glutathione peroxidase 2 is a metabolic driver of the
            tumor immune microenvironment and immune checkpoint inhibitor
            response.
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Lennicke,C., Rahn,J., Wickenhauser,C., Lichtenfels,R., Muller,A.S.,
            Wessjohann,L.A., Kipp,A.P. and Seliger,B.
  TITLE     Loss of epithelium-specific GPx2 results in aberrant cell fate
            decisions during intestinal differentiation
  JOURNAL   Oncotarget 9 (1), 539-552 (2017)
   PUBMED   29416634
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1071)
  AUTHORS   Wu,Y., Wei,F.Y., Kawarada,L., Suzuki,T., Araki,K., Komohara,Y.,
            Fujimura,A., Kaitsuka,T., Takeya,M., Oike,Y., Suzuki,T. and
            Tomizawa,K.
  TITLE     Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is
            Required for Mitochondrial Translation and Is Involved in
            Reversible Infantile Liver Injury
  JOURNAL   PLoS Genet 12 (9), e1006355 (2016)
   PUBMED   27689697
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1071)
  AUTHORS   Muller,M.F., Florian,S., Pommer,S., Osterhoff,M., Esworthy,R.S.,
            Chu,F.F., Brigelius-Flohe,R. and Kipp,A.P.
  TITLE     Deletion of glutathione peroxidase-2 inhibits azoxymethane-induced
            colon cancer development
  JOURNAL   PLoS One 8 (8), e72055 (2013)
   PUBMED   23977205
  REMARK    GeneRIF: GPx2 is up-regulated in dysplastic colonic crypts, where
            it appears to act inhibit apoptosis and, thus, supports tumor
            development.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1071)
  AUTHORS   Tham,D.M., Whitin,J.C., Kim,K.K., Zhu,S.X. and Cohen,H.J.
  TITLE     Expression of extracellular glutathione peroxidase in human and
            mouse gastrointestinal tract
  JOURNAL   Am J Physiol 275 (6), G1463-G1471 (1998)
   PUBMED   9843785
REFERENCE   6  (bases 1 to 1071)
  AUTHORS   Esworthy,R.S., Swiderek,K.M., Ho,Y.S. and Chu,F.F.
  TITLE     Selenium-dependent glutathione peroxidase-GI is a major glutathione
            peroxidase activity in the mucosal epithelium of rodent intestine
  JOURNAL   Biochim Biophys Acta 1381 (2), 213-226 (1998)
   PUBMED   9685647
  REMARK    GeneRIF: GPX2 activity is confined to the mucosal epithelium of the
            GI tract. Activity is detected all along the crypt-villus axis with
            concentration in the crypts. GPX1 activity is found in all layers
            of the gut and all parts of the mucosal epithelium.
REFERENCE   7  (bases 1 to 1071)
  AUTHORS   Tsuji,T., Watanabe,Y., Katoh,H., Sato,K. and Kunieda,T.
  TITLE     Cloning and mapping of the mouse Gpx2 gene encoding
            gastrointestinal glutathione peroxidase
  JOURNAL   J Vet Med Sci 60 (5), 651-654 (1998)
   PUBMED   9637305
REFERENCE   8  (bases 1 to 1071)
  AUTHORS   Chu,F.F., Esworthy,R.S., Ho,Y.S., Bermeister,M., Swiderek,K. and
            Elliott,R.W.
  TITLE     Expression and chromosomal mapping of mouse Gpx2 gene encoding the
            gastrointestinal form of glutathione peroxidase, GPX-GI
  JOURNAL   Biomed Environ Sci 10 (2-3), 156-162 (1997)
   PUBMED   9315306
  REMARK    Review article
REFERENCE   9  (bases 1 to 1071)
  AUTHORS   Brinkmeier,M.L. and Camper,S.A.
  TITLE     Localization of somatostatin receptor genes on mouse chromosomes 2,
            11, 12, 15, and 17: correlation with growth QTLs
  JOURNAL   Genomics 43 (1), 9-14 (1997)
   PUBMED   9226367
REFERENCE   10 (bases 1 to 1071)
  AUTHORS   Chu,F.F., Esworthy,R.S. and Burmeister,M.
  TITLE     The mouse glutathione peroxidase Gpx2 gene maps to chromosome 12;
            its pseudogene Gpx2-ps maps to chromosome 7
  JOURNAL   Genomics 33 (3), 516-518 (1996)
   PUBMED   8661011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CF612186.1, AK131940.1 and
            BX629737.1.
            
            On Apr 13, 2007 this sequence version replaced NM_030677.1.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by
            glutathione, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            is predominantly expressed in the gastrointestinal tract in
            rodents, is localized in the cytoplasm, and whose preferred
            substrate is hydrogen peroxide. Knockout studies in mice lacking
            this gene suggest a role for this isozyme in intestinal
            inflammation and colon cancer development. This isozyme is also a
            selenoprotein, containing the rare amino acid selenocysteine (Sec)
            at its active site. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal. A
            pseudogene of this locus has been identified on chromosome 7.
            [provided by RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK131940.1, BC039658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 9637305
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               CF612186.1         30-214
            186-1065            AK131940.1         63-942
            1066-1071           BX629737.1         1-6                 c
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 33.73 cM"
     gene            1..1071
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /note="glutathione peroxidase 2"
                     /db_xref="GeneID:14776"
                     /db_xref="MGI:MGI:106609"
     exon            1..401
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..752
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /EC_number="1.11.1.9"
                     /EC_number="1.11.1.12"
                     /note="UGA stop codon recoded as selenocysteine;
                     glutathione peroxidase-gastrointestinal; GPx-2; intestinal
                     GPx; phospholipid hydroperoxide glutathione peroxidase
                     GPX2"
                     /codon_start=1
                     /transl_except=(pos:297..299,aa:Sec)
                     /product="glutathione peroxidase 2"
                     /protein_id="NP_109602.2"
                     /db_xref="CCDS:CCDS25996.1"
                     /db_xref="GeneID:14776"
                     /db_xref="MGI:MGI:106609"
                     /translation="MAYIAKSFYDLSAVGLDGEKIDFNTFRGRAVLIENVASLUGTTT
                     RDYNQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLT
                     QKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIG
                     PEGEPFRRYSRSFQTINIEPDIKRLLKVAI"
     exon            402..1067
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      954..1019
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1045..1050
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /note="hexamer: AATAAA"
     polyA_site      1065
                     /gene="Gpx2"
                     /gene_synonym="GI-GPx; GPx-GI; GSHPx-2; GSHPx-GI"
                     /note="major polyA site"
ORIGIN      
        1 gctacagcct tgttcaaaca gttcacaggt gggtacctgt tttttgctaa gtcatcccgg
       61 gaatgctcaa aggccctttg tgaagtcctt tcggtcttct ccggctcctc ctttcttccc
      121 accggtctaa aggacttaag gaggctcaca gagcagggca gggctcactg ctcttcagca
      181 tggcttacat tgccaagtcg ttctacgatc tcagtgccgt tggcctggat ggggagaaga
      241 tagacttcaa tacgttcaga ggcagggctg tgctgattga gaatgtggcg tcactctgag
      301 gaacaactac ccgggactac aaccagctca atgagctgca atgtcgcttt cccaggcgcc
      361 tggtagttct cggcttccct tgcaaccagt tcggacatca ggagaactgt cagaacgagg
      421 agatcctgaa cagcctcaag tatgtccgac ctgggggtgg gtaccagccc acctttagtc
      481 ttacccaaaa gtgtgacgtc aatgggcaga acgagcatcc tgtctttgcc tacctgaaag
      541 acaagctgcc ctacccttat gatgacccgt tctccctcat gaccgatccc aagctcatca
      601 tatggagtcc cgtgcgccgc tcagacgtgt cctggaactt tgagaagttc ctcatagggc
      661 cagaagggga gcccttccgt cgctacagcc gcagcttcca gaccatcaac atcgagcctg
      721 acatcaaacg gctcctcaaa gttgccatct agatgagagc tgctcagccc aggaatctcc
      781 cactgtttcc cctgagcagt cttcctcagg gctcagtgta ccctcgggag accctgggag
      841 accaaggcat tccctgaata tcgtcccctt gccttcccta ccggccattt cctttagctc
      901 cctcaaggct cttggggagt ttgcttgggg ctctaagtct ggggtaggtt ctgggccttc
      961 acagaatgat ggcatcttcc taaacccttc tgggagatgt ctgagaagtt gtgaagggtc
     1021 cagagccagt ctgctttaga gtccaataaa gtgtaggtgt ggcaatgaaa a
//
