LOCUS       NM_153410               3389 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus G-protein signalling modulator 1 (AGS3-like, C.
            elegans) (Gpsm1), transcript variant 1, mRNA.
ACCESSION   NM_153410
VERSION     NM_153410.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Tang,M., Zhang,Y., Zhang,R., Zhang,Y., Zheng,J., Wang,D., Wang,X.,
            Yan,J. and Hu,C.
  TITLE     GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis
            and provokes diet-induced obesity
  JOURNAL   Mol Metab 79, 101839 (2024)
   PUBMED   37979657
  REMARK    GeneRIF: GPSM1 in POMC neurons impairs brown adipose tissue
            thermogenesis and provokes diet-induced obesity.
REFERENCE   3  (bases 1 to 3389)
  AUTHORS   Descovich,C.P., Lough,K.J., Jena,A., Wu,J.J., Yom,J., Spitzer,D.C.,
            Uppalapati,M., Kedziora,K.M. and Williams,S.E.
  TITLE     AGS3 antagonizes LGN to balance oriented cell divisions and cell
            fate choices in mammalian epidermis
  JOURNAL   Elife 12, e80403 (2023)
   PUBMED   37017303
  REMARK    GeneRIF: AGS3 antagonizes LGN to balance oriented cell divisions
            and cell fate choices in mammalian epidermis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3389)
  AUTHORS   Yan,J., Zhang,Y., Yu,H., Zong,Y., Wang,D., Zheng,J., Jin,L., Yu,X.,
            Liu,C., Zhang,Y., Jiang,F., Zhang,R., Fang,X., Xu,T., Li,M., Di,J.,
            Lu,Y., Ma,X., Zhang,J., Jia,W. and Hu,C.
  TITLE     GPSM1 impairs metabolic homeostasis by controlling a
            pro-inflammatory pathway in macrophages
  JOURNAL   Nat Commun 13 (1), 7260 (2022)
   PUBMED   36434066
  REMARK    GeneRIF: GPSM1 impairs metabolic homeostasis by controlling a
            pro-inflammatory pathway in macrophages.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3389)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3389)
  AUTHORS   Sanada,K. and Tsai,L.H.
  TITLE     G protein betagamma subunits and AGS3 control spindle orientation
            and asymmetric cell fate of cerebral cortical progenitors
  JOURNAL   Cell 122 (1), 119-131 (2005)
   PUBMED   16009138
REFERENCE   7  (bases 1 to 3389)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   8  (bases 1 to 3389)
  AUTHORS   Kaushik,R., Yu,F., Chia,W., Yang,X. and Bahri,S.
  TITLE     Subcellular localization of LGN during mitosis: evidence for its
            cortical localization in mitotic cell culture systems and its
            requirement for normal cell cycle progression
  JOURNAL   Mol Biol Cell 14 (8), 3144-3155 (2003)
   PUBMED   12925752
  REMARK    GeneRIF: cortical localization in mitotic cell culture systems and
            requirement for normal cell cycle progression
REFERENCE   9  (bases 1 to 3389)
  AUTHORS   Blumer,J.B., Bernard,M.L., Peterson,Y.K., Nezu,J., Chung,P.,
            Dunican,D.J., Knoblich,J.A. and Lanier,S.M.
  TITLE     Interaction of activator of G-protein signaling 3 (AGS3) with LKB1,
            a serine/threonine kinase involved in cell polarity and cell cycle
            progression: phosphorylation of the G-protein regulatory (GPR)
            motif as a regulatory mechanism for the interaction of GPR motifs
            with Gi alpha
  JOURNAL   J Biol Chem 278 (26), 23217-23220 (2003)
   PUBMED   12719437
REFERENCE   10 (bases 1 to 3389)
  AUTHORS   Peterson,Y.K., Bernard,M.L., Ma,H., Hazard,S. 3rd, Graber,S.G. and
            Lanier,S.M.
  TITLE     Stabilization of the GDP-bound conformation of Gialpha by a peptide
            derived from the G-protein regulatory motif of AGS3
  JOURNAL   J Biol Chem 275 (43), 33193-33196 (2000)
   PUBMED   10969064
  REMARK    GeneRIF: The GPR motif in this protein and other proteins is
            actually associated with activity as a GDI - guanine nucleotide
            dissociation inhibitor and not GTPase activator as initially
            suggested.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CA317518.1, AK048691.1 and BC071197.1.
            
            On Nov 23, 2010 this sequence version replaced NM_153410.4.
            
            Transcript Variant: This variant (1) has an alternate 5' terminus
            which results in the use of an alternate tranlation initiation
            site, compared to variant 3. Variant 1 encodes isoform 1 which has
            a shorter and distinct N-terminus, compared to isoform 3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048691.1, AK082985.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               CA317518.1         19-219
            202-3364            AK048691.1         196-3358
            3365-3389           BC071197.1         3431-3455
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 18.73 cM"
     gene            1..3389
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /note="G-protein signalling modulator 1 (AGS3-like, C.
                     elegans)"
                     /db_xref="GeneID:67839"
                     /db_xref="MGI:MGI:1915089"
     exon            1..188
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2142
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     activator of G-protein signaling 3; G-protein-signaling
                     modulator 1"
                     /codon_start=1
                     /product="G-protein-signaling modulator 1 isoform 1"
                     /protein_id="NP_700459.2"
                     /db_xref="CCDS:CCDS15800.1"
                     /db_xref="GeneID:67839"
                     /db_xref="MGI:MGI:1915089"
                     /translation="MASPAPPVAEELPGPASRRLYSRMEASCLELALEGERLCKAGDF
                     KAGVAFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLKEYARALQFHKHDLLLARTIGDR
                     MGEAKASGNLGNTLKVLGRFDEAIVCCQRHLDIAQEQGDKVGEARALYNIGNVYHAKG
                     KQLSWNAAQDPGHLPPDVRETLHRASEFYERNLSLVKELGDRAAQGRAYGNLGNTHYL
                     LGNFTEATTFHKERLAIAKEFGDKAAERRAYSNLGNAHIFLGRFDVAAEHYKKTLQLS
                     RQLRDQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLVIAQELADRVGEGRACWSLGN
                     AYVSMGSPAQALTFAKKHLQISQEIGDRNGELTARMNIAHLQLALGRLTSPAAAEKPD
                     LAGYEAQGARPKRTQRLSAETWDLLRLPLDREQNGETHHTGDWRGPGRDSLPLPMRSR
                     KYQEGPDAIERRPREGSHSPLDSADVRVQVPRTGIPRAPSSDEECFFDLLSKFQSSRM
                     DDQRCPLEEGQAGAAEATAAPSVEDRAAQSSVTASPQTEEFFDLIASSQSRRLDDQRA
                     SVGSLPGLRITLNNVGHLRGDGDAQEPGDEFFNMLIKYQSSRIDDQRCPPPDVLPRGP
                     TMPDEDFFSLIQRVQAKRMDEQRVDLAGSPEQEASGLPDPQQQCPPGAS"
     exon            189..410
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            411..546
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            547..689
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            690..813
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            814..929
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1085
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1194
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1318
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1389
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1569
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1726
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1935
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..3366
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3347..3352
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /note="hexamer: AATAAA"
     polyA_site      3366
                     /gene="Gpsm1"
                     /gene_synonym="1810037C22Rik; Ags3"
                     /note="major polyA site"
ORIGIN      
        1 cttcctcggc gtctcccgcg gacgcgcgga caggcggacg gacgcgcgga caggcgggca
       61 gatcggcagg cggacggccg cagagcgggg cggtgctcct gctcggcgcg tccccgactc
      121 atggcgagcc cggccccgcc cgtggccgag gagctcccgg gcccggcctc caggcgcctc
      181 tactccagga tggaggcctc ctgtctagag ctggccctgg aaggggagcg gctgtgcaag
      241 gcgggggact tcaaggcagg tgtggctttc ttcgaggctg ctgtgcaggt gggcactgag
      301 gacctgaaga cactgagtgc catctacagc cagctgggca atgcctactt ctacctgaag
      361 gagtatgccc gggccctgca gttccacaaa catgacttgc tgctggcacg gaccattggt
      421 gaccgcatgg gggaggccaa ggctagtggg aacctgggca acacactcaa ggtcctaggc
      481 cgattcgatg aggcaatcgt ctgctgccaa cgacacttgg acattgccca ggagcagggg
      541 gacaaggttg gggaggcgag agcactctac aacattggaa atgtgtacca cgccaagggc
      601 aaacagcttt cctggaatgc tgcacaggac cccgggcacc tgccacctga tgtccgcgag
      661 acactgcaca gggcctctga gttttatgag aggaacctgt ctttggtgaa ggaactaggc
      721 gaccgggcgg cccagggcag ggcctatggc aacctgggta acacccacta cctactggga
      781 aacttcacgg aggccacaac cttccacaaa gagcgcctgg ccatcgccaa ggagtttggg
      841 gacaaggcag ctgagcggag ggcctacagc aacctgggca atgctcacat cttcttgggg
      901 cgctttgatg tggctgctga acattacaag aagacgctgc agctgtctcg gcagctccgg
      961 gaccaggcag tggaggctca ggcttgctac agcctgggca acacctacac actgctacag
     1021 gactacgagc gtgctgctga gtaccacctg cggcacctag tcattgccca ggagctggct
     1081 gatagggtgg gagagggccg agcatgctgg agcctgggga acgcctatgt gtccatgggg
     1141 agccctgcac aggccttgac ctttgccaag aaacatctgc agatctccca ggagattgga
     1201 gaccgaaacg gagaactgac agcccgcatg aatattgccc acctgcagct ggccctgggc
     1261 cggctgacta gcccagcagc agcggagaag ccagatctgg ctggctatga ggcacaagga
     1321 gcaagaccca aacggacaca gaggctgagt gccgagacct gggacctgct gcggctcccc
     1381 ctggaccggg agcagaatgg agagacccac cacacagggg actggagggg tccaggcagg
     1441 gactcgcttc ccctccccat gaggagcagg aagtaccagg aaggtccaga tgccattgag
     1501 aggagacccc gggagggtag tcactctcca ctggacagtg ctgatgtaag ggtacaggtg
     1561 cctcgtacgg gtattcccag ggccccgtct tctgacgagg agtgtttctt tgacctgctg
     1621 agtaagttcc agagcagtcg catggatgac cagcgctgtc ccctggagga aggccaggct
     1681 ggggctgctg aggccacagc tgccccatcc gtggaggata gagcagctca gtcctccgtg
     1741 acagcttcac cacagacaga ggagttcttt gacctcattg ccagctccca gagccgccgg
     1801 ctggacgacc agagggctag cgtaggcagc ctgcctgggc tacgcatcac cctcaacaat
     1861 gtggggcacc tccgaggcga cggggacgcc caggagccgg gggatgagtt tttcaacatg
     1921 cttatcaaat accagtcctc caggattgat gaccagcgct gcccaccccc tgatgtgctg
     1981 ccccgtggcc ctaccatgcc tgatgaggat ttcttcagcc ttatccagag ggtgcaggct
     2041 aagcggatgg atgagcagcg tgtggacctt gctgggagtc cagagcaaga ggccagtggg
     2101 ctgcctgatc cccagcagca gtgtccaccg ggtgccagct aaggcctcgc ccctacagcc
     2161 agccataccc tactctggac tctgtaggct cacggttgtc cacagtggcc atgatccccc
     2221 aataagccaa atcttcccaa ggccatgatg gacagccagc tcagccccag ccccttccca
     2281 ctgagccaga tggtgggtgc acaaggcccc cccatcccag gtggtgggtg caggctagag
     2341 ctgctatatg ggggagggca tgcttctgtc cccatggatc tccacagctt cctgcaggcc
     2401 tgccctgccc tgccctaggc cgggccttca gcatgttggc cccgagcccc ggtgctgtcc
     2461 agagtctttt ccctcaagcc ctgccctgcc aaatgtgaaa ctctgccaac tctcccaagg
     2521 cccccagagg ggcttcaagg acttcttctg gtgggggcta cagtgctaag gcatcttctg
     2581 tcccccagct ggtcctggga tggctatgaa ggaggtacac tctgatctgc ctcacgaccc
     2641 cagggcaggc actatcctgg cctggctgtg cccaaccctg ctgagggaga ttggcagcta
     2701 caaagtccag acaccctgac ctggtagtcc ccagccttgc acttggcctt tgaggaaaga
     2761 tggagcttgc ccctggcaga ggggagaggc aggtctccaa gcatagggtc ttgtactagg
     2821 agtgctgagc tctggctggt agtggacgtt catgagggat cctctcaact agatccttca
     2881 gtcctcttag tgagcatcta ccttctatta ctagggagat gatatcttgg ctttctcccc
     2941 aaggaggggt ccatatctta attggacaca gggcactgta gttggggtgg agactcagcc
     3001 agaactgccg gggcatccat gagacagacc cttcgggacc gtggtcctag ttcttgccta
     3061 caagtcctat aggtagccct actggagggc tctgtacaac tgtctaaccc agggcctctt
     3121 acccagaggg ccagggcaag ggaaactgag gacccaactt ctgagaggga gaggcaacat
     3181 cactctgaga gcagaaacag atactagagc acagggtctc gggcgggaca tggtggccgc
     3241 cccgcatact tgaatgtaca tgcgtattta ttgcttacat gtgtttgcca tgttgttcat
     3301 gggtcctttc tgacccgaga ggtacatttg ttttgtttta cccaaaaata aaagtctgcc
     3361 aagtgaaaaa aaaaaaaaaa aaaaaaaaa
//
