LOCUS       NM_022420               4525 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus G protein-coupled receptor, family C, group 5, member
            B (Gprc5b), transcript variant 2, mRNA.
ACCESSION   NM_022420
VERSION     NM_022420.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Xu,W., Nelson-Maney,N.P., Balint,L., Kwon,H.B., Davis,R.B.,
            Dy,D.C.M., Dunleavey,J.M., St Croix,B. and Caron,K.M.
  TITLE     Orphan G-Protein Coupled Receptor GPRC5B Is Critical for Lymphatic
            Development
  JOURNAL   Int J Mol Sci 23 (10), 5712 (2022)
   PUBMED   35628521
  REMARK    GeneRIF: Orphan G-Protein Coupled Receptor GPRC5B Is Critical for
            Lymphatic Development.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4525)
  AUTHORS   Freundt,G.V., von Samson-Himmelstjerna,F.A., Nitz,J.T., Luedde,M.,
            Waltenberger,J., Wieland,T., Frey,N., Preusch,M. and Hippe,H.J.
  TITLE     The orphan receptor GPRC5B activates pro-inflammatory signaling in
            the vascular wall via Fyn and NFkappaB
  JOURNAL   Biochem Biophys Res Commun 592, 60-66 (2022)
   PUBMED   35033869
  REMARK    GeneRIF: The orphan receptor GPRC5B activates pro-inflammatory
            signaling in the vascular wall via Fyn and NFkappaB.
REFERENCE   4  (bases 1 to 4525)
  AUTHORS   Takizawa,N., Hironaka,T., Mae,K., Ueno,T., Horii,Y., Nagasaka,A.
            and Nakaya,M.
  TITLE     GPRC5B promotes collagen production in myofibroblasts
  JOURNAL   Biochem Biophys Res Commun 561, 180-186 (2021)
   PUBMED   34023784
  REMARK    GeneRIF: GPRC5B promotes collagen production in myofibroblasts.
REFERENCE   5  (bases 1 to 4525)
  AUTHORS   Carvalho,J., Chennupati,R., Li,R., Gunther,S., Kaur,H., Zhao,W.,
            Tonack,S., Kurz,M., Mosslein,N., Bunemann,M., Offermanns,S. and
            Wettschureck,N.
  TITLE     Orphan G Protein-Coupled Receptor GPRC5B Controls Smooth Muscle
            Contractility and Differentiation by Inhibiting Prostacyclin
            Receptor Signaling
  JOURNAL   Circulation 141 (14), 1168-1183 (2020)
   PUBMED   31941358
REFERENCE   6  (bases 1 to 4525)
  AUTHORS   Kim,Y.J., Sano,T., Nabetani,T., Asano,Y. and Hirabayashi,Y.
  TITLE     GPRC5B activates obesity-associated inflammatory signaling in
            adipocytes
  JOURNAL   Sci Signal 5 (251), ra85 (2012)
   PUBMED   23169819
  REMARK    GeneRIF: GPRC5B activates obesity-associated inflammatory signaling
            in adipocytes.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4525)
  AUTHORS   Sano,T., Kim,Y.J., Oshima,E., Shimizu,C., Kiyonari,H., Abe,T.,
            Higashi,H., Yamada,K. and Hirabayashi,Y.
  TITLE     Comparative characterization of GPRC5B and GPRC5CLacZ knockin mice;
            behavioral abnormalities in GPRC5B-deficient mice
  JOURNAL   Biochem Biophys Res Commun 412 (3), 460-465 (2011)
   PUBMED   21840300
  REMARK    GeneRIF: GPRC5B has important roles for animal behavior controlled
            by the central nervous system.
REFERENCE   8  (bases 1 to 4525)
  AUTHORS   Cool,B.H., Chan,G.C., Lee,L., Oshima,J., Martin,G.M. and Hu,Q.
  TITLE     A flanking gene problem leads to the discovery of a Gprc5b splice
            variant predominantly expressed in C57Bl/6J mouse brain and in
            maturing neurons
  JOURNAL   PLoS One 5 (4), e10351 (2010)
   PUBMED   20436672
  REMARK    GeneRIF: Gprc5b-v2 may play a role during brain maturation and in
            matured brain, possibly through the regulation of neuronal
            morphology and protein-protein interaction
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4525)
  AUTHORS   Imanishi,S., Sugimoto,M., Morita,M., Kume,S. and Manabe,N.
  TITLE     Changes in expression and localization of GPRC5B and RARalpha in
            the placenta and yolk sac during middle to late gestation in mice
  JOURNAL   J Reprod Dev 53 (5), 1131-1136 (2007)
   PUBMED   17652913
  REMARK    GeneRIF: GPRC5B is involved in retinoic acid -dependent
            morphogenesis/angiogenesis and regulation of extracellular matrix
            synthesis in the murine placenta and yolk sac.
REFERENCE   10 (bases 1 to 4525)
  AUTHORS   Xu,J., Tian,J. and Shapiro,S.D.
  TITLE     Normal lung development in RAIG1-deficient mice despite unique lung
            epithelium-specific expression
  JOURNAL   Am J Respir Cell Mol Biol 32 (5), 381-387 (2005)
   PUBMED   15677768
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151534.2.
            
            On Jul 29, 2009 this sequence version replaced NM_022420.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region, which results in a frameshift, compared to
            variant 1. It encodes isoform 2, which has a shorter and distinct
            C-terminus, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF378831.1, AK032047.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-172               AC151534.2         172532-172703       c
            173-1203            AC151534.2         161107-162137       c
            1204-1361           AC151534.2         154005-154162       c
            1362-4525           AC151534.2         149532-152695       c
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.72 cM"
     gene            1..4525
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="G protein-coupled receptor, family C, group 5,
                     member B"
                     /db_xref="GeneID:64297"
                     /db_xref="MGI:MGI:1927596"
     exon            1..172
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /inference="alignment:Splign:2.1.0"
     exon            173..1203
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..1406
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; RAIG-2; retinoic acid-induced gene 2 protein;
                     hypothetical protein, clone 2-63"
                     /codon_start=1
                     /product="G-protein coupled receptor family C group 5
                     member B isoform 2 precursor"
                     /protein_id="NP_071865.1"
                     /db_xref="CCDS:CCDS21777.1"
                     /db_xref="GeneID:64297"
                     /db_xref="MGI:MGI:1927596"
                     /translation="MFLVLERKMRTHQVFPLPLLLVIASVASENASTSRGCGLDLLPQ
                     YVSLCDLDAIWGIVVEAVAGAGALITLLLMLILLVRLPFIKDKERKRPVCLHFLFLLG
                     TLGLFGLTFAFIIQMDETICSIRRFLWGVLFALCFSCLLSQAWRVRRLVRQGTSPASW
                     QLVSLALCLMLVQVIIATEWLVLTVLRDTKPACAYEPMDFVMALIYDMVLLAITLAQS
                     LFTLCGKFKRWKVNGAFILVTTFLSALIWVVWMTMYLFGNSLIKQGDAWSDPTLAITL
                     AASGWVFVIFHAIPEIHYTLLPPLQENPPNYFDTSQPRMRETAFDEEMHLPRAYMENK
                     AFSMDEHNAALRSAVGFSNGSLEQRSSSLGKKPSSLGNRPSAPFRSNVYQPTEMAVVL
                     NGGTIPTAPPSHTGRHHW"
     exon            1204..1361
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..4525
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1426..1431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="hexamer: AATAAG"
     polyA_site      1446
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="major polyA site"
     regulatory      2893..2898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="hexamer: AATAAA"
     polyA_site      2915
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
     regulatory      4499..4504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="hexamer: AATAAA"
     polyA_site      4523
                     /gene="Gprc5b"
                     /gene_synonym="Raig2"
                     /note="major polyA site"
ORIGIN      
        1 agcgcgggcc gggccagggg gcgcctcgcg cggccgcagc tcggaagggc gatgcagtga
       61 ggcgggctcg gctggcgggc gtgcgcggag cgggggccgc gggcgcgctg cagagatgtg
      121 acttgggccc agggccagca ggagcgtcgg cgctgcgggg acgcgagggt cgaatgttcc
      181 tggtgttaga gagaaagatg agaacccatc aagtgtttcc cttgcccctg ctcctggtga
      241 ttgcctccgt ggcttcagag aacgccagca cgtcccgggg ctgtggactg gaccttcttc
      301 ctcagtacgt gtccctgtgc gacctggacg ccatctgggg catcgtggtg gaggcagtgg
      361 ccggggcggg ggccctgatc acactgcttc tgatgctcat tctcctagtg agactaccct
      421 tcatcaagga caaggaaagg aagcggcctg tgtgcctcca tttcctcttc ctgctgggga
      481 ccctgggcct ctttggcctg acgtttgcct tcatcatcca gatggacgag acaatctgct
      541 ccatccgacg cttcctctgg ggtgtcctct tcgcgctctg cttttcctgc ctgctgagcc
      601 aggcgtggcg ggtgcggagg ctggtgcgcc agggcacaag cccggccagc tggcagctgg
      661 tgagcctggc actgtgcctg atgctggtgc aggtcatcat tgccactgag tggctggtgc
      721 tgactgtgct gcgtgacacg aagccagcct gcgcctacga gcccatggat tttgtgatgg
      781 cgctcatcta cgacatggtg ctgctggcca tcaccctggc ccagtccctc ttcacgctgt
      841 gtggcaagtt caaacggtgg aaggtgaacg gagccttcat cctcgtcact accttcctct
      901 ctgcgctcat ctgggtggtc tggatgacca tgtacctctt cggcaactca ttaattaagc
      961 agggagatgc ctggagcgac cccaccttgg ccattacact ggcagccagc ggatgggtct
     1021 ttgtcatctt ccatgccatt ccggagatcc actacaccct tctcccaccc ctgcaggaga
     1081 acccacccaa ctacttcgac acttcacagc ccaggatgcg ggagacagca tttgatgagg
     1141 aaatgcacct gcctcgggcc tacatggaga acaaggcctt ctcaatggat gaacataacg
     1201 cagctctccg atcagcagtg ggcttttcca atggaagctt ggagcaaaga tctagcagcc
     1261 tgggaaagaa gcccagcagc ttgggaaacc ggcccagtgc accgttcaga agcaatgtgt
     1321 atcagccgac cgagatggcc gtcgtgctca atggagggac tatcccaact gctccgccat
     1381 ctcacacggg aagacaccat tggtgaaaga ccttaagttc cagagaataa gaatatttct
     1441 taccgatttt attccctggc tgtgtctttc ttgagggcga aatcactaac catggcagag
     1501 acaggccacc cggaagccag gagatctgag aagtcccacc aaggggattt cgtgtaaatg
     1561 tgacatatgg atgaactgga aagctaacag tgaccgccct cccctcccct gccacacatg
     1621 caaacacgga atcgtcctgc aaactaaatc agagctaact gccaatagtc ttagactcac
     1681 tcagaagcgt ggctgggaaa ctgagtcatt cagcaggggg caggacagag aagaagtcac
     1741 agctgctgta ccagactatc ttggcttatt atgtgagggg ttggagccag cctgtcaccc
     1801 ccaccctcct caccaaatgc tggtccccag agccatcttt tggagagaac tgtgcccttg
     1861 aggttaactc atggatcatt ctccctggct tttgcttgcc tggtggccca tgcagttcag
     1921 gagactttaa gaggttgtgg ctatgattct caaggcccag ctggatggtc agggagctgt
     1981 gtagccagta ggaatggagg aaccatagaa cgtgccaaag aagagaaggt actctggact
     2041 ttgaagtgac catcacatgt agacagaggt gaccaaccgt tgttccttct gtgggcctct
     2101 tgctctacca agcaccccac ctaccccaac ctcccctaca gagtgataga aatatccctc
     2161 ctccttcctc ttgcaggtgc tcctccatgc cttcccccaa ccctagccca ccctcaccct
     2221 ccacccccta acccccaagt tcattaccat cttcatttca gaagctcaag tagggtcgcc
     2281 agcttgaagc ttcaggggag gcaggctgaa aggcaggcgc catggcgcct tttcgtaatg
     2341 atgtcctatg gctgggttcc ccaggggtga cctctaggat cgagagatgg catcctgttg
     2401 cccagctgcc agtggcttct gggatatttc cccttcatgt gggtagaagt ccaggataca
     2461 atgagctcca tgaaggtttc tggctgcttt tggagggtat ggagaacgtc ttgattctgg
     2521 ttttccgatg gctctatgga tgtggccccc ttcacaactg tcatttgtca tggcttccat
     2581 ctgtcctgag caagtcattc ctttgttgct aggcatctcc agcatccctg ccattaaaag
     2641 ccctgcgttc tctgcactgt ttggccagca taatctctag caacctttcc atgctgagtt
     2701 tgaacctgac catatggact gtataaacaa gggttgcagc ctctgcatac actcgaatcg
     2761 ctacattgct ttttctataa aaactaccca taagccttta acctttaatg caaattttta
     2821 aaaggttagt gtttgggggg gggggtgggg actgacctcg tcttaagcca gtacatctga
     2881 gctgagtgtt ttaataaatc tgatatttct caaggcccca gtctctgctg tgtcccctcc
     2941 ctgccctagc ctatccttgt agagccttag ggaggtgtgg ccaatctggc cagcgcttgg
     3001 ctctcctgca agttttcctt acagctagca catcaccatg catgggaacc agaaagcatg
     3061 gcacccaaag gtcacccctt cccaacctcc tgagatgcct cacccccatc cagagcacgc
     3121 gcttgctttc aatcagtgct taaggtccat caagtggtgg gcttagaact tagaggcctg
     3181 aggaggtgtg ggaaggaaag aaagctaggg ctgtgtgggg ctggggctgg ggctgttgca
     3241 tgcactaccc tgcccttctg caaagatatc tccgagcacc tggggccagt ccggtgacaa
     3301 acgtaagcgc tccgcattct tgtgccagga aatggctgtc catccccgct ctctggagtc
     3361 ctttggagct ttctgatgtc accaataact cggtctgcaa aaccccccct tgcagaaggg
     3421 agaagctgac atgattgacg gttttctctc taagctttgt tgtccacgta taaggtgagc
     3481 accccaccgt gcagacggct gtgcagaaag cagcttgttt ctgcactgtg aacacagaac
     3541 cacgctgagc cacaggaaaa cccatcgctt agtgaggcct ttcagttttg ctttttttcc
     3601 tttccaatcc tgataggtta ttacgctgct gggctttgtt ttatcttggc tttaagattc
     3661 agggtgggct gaatgttttg acatggaggg cggaagtagg agcaaaagaa gtagggccac
     3721 atctactact attctgagtt cgagttcacc tggagaggac cacgaggcaa ctccacccag
     3781 agactgtggc agccagcttc tgacagagca aggctgaagt gcagaagccc acggcacctg
     3841 tttaacagtt gatagacccg aggcttggtt ggggaaacgg tctagaaagt cacctgggaa
     3901 taaccccaag ctgcgagcac aacttggcat gccaccatat tgggactata acagccactc
     3961 tgtcccctcc tgagagaaga ctccacagag gtctggagtc agttgccaag gtagcgggac
     4021 aatggcagag gggaggggct ccaatgtgcc agctgtttca gaagttaaga tctggttatt
     4081 gctccacccc ccacagatga gatcaaggac agccaacaag tgtagacggg tgggggaggg
     4141 gttgtctttt gaatttgcca aatggcagag tagaagagga cactggcatt tacacatcca
     4201 aggatgcagc gaccatgtga gctgatgaga gcccttggca ggtgctttat tcataaagag
     4261 ctatggctcg ttttttgata gataatatat atttattaaa tgtattaatg tgtattttat
     4321 aatgtaccct cactcttccc cttatcctgg ctgacttgca tacttataaa caaaaattgt
     4381 ttgagaaatt tcaagacaat cagtatgtac agggctgtat ataaaatatc acagacttta
     4441 aaggttgtta agcatgggca gataggtttt attttcaaaa tgctgttctt aacaagaaaa
     4501 taaaggcttt actatttacc taaga
//
