LOCUS       NM_008152               2153 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus G-protein coupled receptor 65 (Gpr65), mRNA.
ACCESSION   NM_008152
VERSION     NM_008152.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Bagchi,S., Yuan,R., Huang,H.L., Zhang,W., Chiu,D.K., Kim,H.,
            Cha,S.L., Tolentino,L., Lowitz,J., Liu,Y., Moshnikova,A.,
            Andreev,O., Plevritis,S. and Engleman,E.G.
  TITLE     The acid-sensing receptor GPR65 on tumor macrophages drives tumor
            growth in obesity
  JOURNAL   Sci Immunol 9 (100), eadg6453 (2024)
   PUBMED   39423285
  REMARK    GeneRIF: The acid-sensing receptor GPR65 on tumor macrophages
            drives tumor growth in obesity.
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Fan,J., Liu,J., Zhang,B., Wang,X., Wang,X., Liang,J., Li,Y.,
            Zhang,Y., Zhang,C., Yu,S., Li,T. and Yang,X.
  TITLE     GPR65 contributes to constructing immunosuppressive
            microenvironment in glioma
  JOURNAL   Neurosurg Rev 47 (1), 417 (2024)
   PUBMED   39123083
  REMARK    GeneRIF: GPR65 contributes to constructing immunosuppressive
            microenvironment in glioma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2153)
  AUTHORS   Neale,I., Reddy,C., Tan,Z.Y., Li,B., Nag,P.P., Park,J., Park,J.,
            Carey,K.L., Graham,D.B. and Xavier,R.J.
  TITLE     Small-molecule probe for IBD risk variant GPR65 I231L alters
            cytokine signaling networks through positive allosteric modulation
  JOURNAL   Sci Adv 10 (29), eadn2339 (2024)
   PUBMED   39028811
REFERENCE   4  (bases 1 to 2153)
  AUTHORS   Caratis,F., Opielka,M., Hausmann,M., Velasco-Estevez,M., Rojek,B.,
            de Valliere,C., Seuwen,K., Rogler,G., Karaszewski,B. and
            Rutkowska,A.
  TITLE     The proton-sensing receptors TDAG8 and GPR4 are differentially
            expressed in human and mouse oligodendrocytes: Exploring their role
            in neuroinflammation and multiple sclerosis
  JOURNAL   PLoS One 19 (3), e0283060 (2024)
   PUBMED   38527054
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2153)
  AUTHORS   Schappe,M.S., Brinn,P.A., Joshi,N.R., Greenberg,R.S., Min,S.,
            Alabi,A.A., Zhang,C. and Liberles,S.D.
  TITLE     A vagal reflex evoked by airway closure
  JOURNAL   Nature 627 (8005), 830-838 (2024)
   PUBMED   38448588
REFERENCE   6  (bases 1 to 2153)
  AUTHORS   Ishii,S., Kihara,Y. and Shimizu,T.
  TITLE     Identification of T cell death-associated gene 8 (TDAG8) as a novel
            acid sensing G-protein-coupled receptor
  JOURNAL   J Biol Chem 280 (10), 9083-9087 (2005)
   PUBMED   15618224
REFERENCE   7  (bases 1 to 2153)
  AUTHORS   Radu,C.G., Nijagal,A., McLaughlin,J., Wang,L. and Witte,O.N.
  TITLE     Differential proton sensitivity of related G protein-coupled
            receptors T cell death-associated gene 8 and G2A expressed in
            immune cells
  JOURNAL   Proc Natl Acad Sci U S A 102 (5), 1632-1637 (2005)
   PUBMED   15665078
REFERENCE   8  (bases 1 to 2153)
  AUTHORS   Malone,M.H., Wang,Z. and Distelhorst,C.W.
  TITLE     The glucocorticoid-induced gene tdag8 encodes a pro-apoptotic G
            protein-coupled receptor whose activation promotes
            glucocorticoid-induced apoptosis
  JOURNAL   J Biol Chem 279 (51), 52850-52859 (2004)
   PUBMED   15485889
  REMARK    GeneRIF: TDAG8 is a regulator of glucocorticoid-induced apoptosis
REFERENCE   9  (bases 1 to 2153)
  AUTHORS   Wang,J.Q., Kon,J., Mogi,C., Tobo,M., Damirin,A., Sato,K.,
            Komachi,M., Malchinkhuu,E., Murata,N., Kimura,T., Kuwabara,A.,
            Wakamatsu,K., Koizumi,H., Uede,T., Tsujimoto,G., Kurose,H.,
            Sato,T., Harada,A., Misawa,N., Tomura,H. and Okajima,F.
  TITLE     TDAG8 is a proton-sensing and psychosine-sensitive
            G-protein-coupled receptor
  JOURNAL   J Biol Chem 279 (44), 45626-45633 (2004)
   PUBMED   15326175
  REMARK    GeneRIF: TDAG8 is one of the proton-sensing GPCRs coupling to
            adenylyl cyclase and psychosine, and its related lysosphingolipids
            behave as if they were antagonists against protein-sensing
            receptors, including TDAG8, GPR4, and OGR1.
REFERENCE   10 (bases 1 to 2153)
  AUTHORS   Choi,J.W., Lee,S.Y. and Choi,Y.
  TITLE     Identification of a putative G protein-coupled receptor induced
            during activation-induced apoptosis of T cells
  JOURNAL   Cell Immunol 168 (1), 78-84 (1996)
   PUBMED   8599842
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK134113.1, AK142853.1 and BF453845.1.
            
            On Nov 3, 2012 this sequence version replaced NM_008152.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134113.1, U39827.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AK134113.1         2-38
            38-1464             AK142853.1         2-1428
            1465-2049           AK134113.1         1464-2048
            2050-2153           BF453845.1         175-278
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 49.84 cM"
     gene            1..2153
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /note="G-protein coupled receptor 65"
                     /db_xref="GeneID:14744"
                     /db_xref="MGI:MGI:108031"
     exon            1..80
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /inference="alignment:Splign:2.1.0"
     exon            81..2147
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /inference="alignment:Splign:2.1.0"
     CDS             515..1528
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /note="G-protein coupled receptor 25; T-cell
                     death-associated gene 8 protein"
                     /codon_start=1
                     /product="psychosine receptor"
                     /protein_id="NP_032178.1"
                     /db_xref="CCDS:CCDS26096.1"
                     /db_xref="GeneID:14744"
                     /db_xref="MGI:MGI:108031"
                     /translation="MAMNSMCIEEQRHLEHYLFPVVYIIVFIVSVPANIGSLCVSFLQ
                     AKKENELGIYLFSLSLSDLLYALTLPLWINYTWNKDNWTFSPTLCKGSVFFTYMNFYS
                     STAFLTCIALDRYLAVVYPLKFSFLRTRRFAFITSLSIWILESFFNSMLLWKDETSVE
                     YCDSDKSNFTLCYDKYPLEKWQINLNLFRTCMGYAIPLITIMICNHKVYRAVRHNQAT
                     ENSEKRRIIKLLASITLTFVLCFTPFHVMVLIRCVLERDMNVNDKSGWQTFTVYRVTV
                     ALTSLNCVADPILYCFVTETGRADMWNILKLCTRKHNRHQGKKRDILSVSTRDAVELE
                     IID"
     regulatory      2041..2046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /note="hexamer: AGTAAA"
     polyA_site      2057
                     /gene="Gpr65"
                     /gene_synonym="Dig1; Gpcr25; TDAG8"
                     /note="major polyA site"
ORIGIN      
        1 aacactgctg gtttctcttc ttgatacaag cagatttgcc agcctcctca gtcaagagaa
       61 gcatccctcc agaaacaggg aaacatgaca cttttgaaag aatgccaaac ggcgtgaaaa
      121 taaaaacaga gcattcccat ttgcaccgac caatctccaa tctcctgtaa gattcaaaag
      181 ggcaagcaag aggcggtgac cgttcacgaa agctaaaatc ccatgctatt gaacatgaag
      241 acttctgatg cttaaatctc attaactgct ttaagtcact cccaggagct tggatcccaa
      301 cttctagcag taatagtctg tgtaaaaaaa aaaaaaaaat cagtctacaa ccactctcta
      361 aatgcatgga tgaactcatc agaacatcaa aacccaagga aaccctaaga gagaagaatt
      421 ctaataaaaa gaattttaca ttgaaaactt acaaggcaag gtccctttcc ctgctgacag
      481 cctaagaagt gatgtaactg ccactgtgaa gaccatggcg atgaacagca tgtgcattga
      541 agagcagcgc cacctcgaac actatttgtt cccggtggtc tacataattg tgtttatagt
      601 cagcgtccca gccaacatcg gatctttatg cgtatccttt ctgcaagcga agaaggaaaa
      661 tgagctaggg atttacctct tcagtctgtc cctgtcagac ctgctgtatg cgctgactct
      721 gcccctctgg atcaattaca cttggaataa agacaactgg actttctctc ccaccttgtg
      781 caaaggaagc gttttcttca cctacatgaa cttttacagc agcacggcgt tcctcacttg
      841 cattgccctg gaccgctatt tagcagtcgt ctaccctctg aagttttcct tcctaagaac
      901 gagaagattc gcgtttatta ccagcctctc catctggata ttagagtcct tctttaactc
      961 tatgcttctg tggaaagatg aaacgagtgt tgaatattgt gactcggaca aatctaattt
     1021 cactctctgc tatgacaaat accctctgga gaaatggcag ataaacctca acctgtttcg
     1081 gacgtgcatg ggctacgcaa tacccttgat caccatcatg atctgcaacc ataaagtcta
     1141 ccgagctgtg cggcacaacc aagccacgga aaacagcgag aagagaagga tcataaagtt
     1201 gcttgctagc atcacgttga ctttcgtcct atgctttacc cccttccacg tgatggtgct
     1261 catccgctgc gttttagagc gcgacatgaa cgtcaatgac aagtctggat ggcagacgtt
     1321 tacggtgtac agagtcacag tagccctgac gagtctaaac tgtgttgccg atcccattct
     1381 gtactgcttt gtgactgaga cggggagagc tgatatgtgg aacatattaa aattgtgtac
     1441 taggaaacac aatagacacc aagggaaaaa aagggacata ctttctgtgt ccacaagaga
     1501 tgctgtagaa ttagagatta tagactaaga ggtggaggca ggttaagtta catggtatta
     1561 tttaatgaaa cttacatttt ggaaaagaaa tctggcatag tagaacccag tggaaatagt
     1621 ttgaaggtac attgtatgac tcctatgttg gctttattaa gtaaggtata gaaatgtatt
     1681 atcttgtatg tattctaatg actaggcatc attgttttag taccaattct ctttgcctct
     1741 atgttataac ccctaagaag cacgcgggac tgttcgtctt taaatcagtg gccattctat
     1801 ctgactacta tgactttttg ttgttgttct gctttgggtt ttcagtctgc ctgcatcagt
     1861 cttctcctct gtatacgtct gtcttcaaca aatgtaagga ctaaataccc ctcccgatca
     1921 catccattat caaggatttg aagccactcc atgtactggg ttataaaaga aatgttctca
     1981 tgaactttca tgaagtttac atacctttgg ggatctagtc accgagtcac ataaagtaaa
     2041 agtaaatgga ttttgtattt tggtcttgat ttctttatat ttatagctga atatgtttat
     2101 atggatgagt ttccgtccag acagataaat atttatgggc aatcttaaaa aaa
//
