LOCUS       NM_175668               2884 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus G protein-coupled receptor 4 (Gpr4), mRNA.
ACCESSION   NM_175668
VERSION     NM_175668.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Gonye,E.C., Shi,Y., Li,K., Clements,R.T., Xu,W. and Bayliss,D.A.
  TITLE     Intrinsic Molecular Proton Sensitivity Underlies GPR4 Effects on
            Retrotrapezoid Nucleus Neuronal Activation and CO2-Stimulated
            Breathing
  JOURNAL   J Neurosci 44 (36), e0799242024 (2024)
   PUBMED   39107057
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Gonye,E.C., Dagli,A.V., Kumar,N.N., Clements,R.T., Xu,W. and
            Bayliss,D.A.
  TITLE     Expression of endogenous epitope-tagged GPR4 in the mouse brain
  JOURNAL   eNeuro 11 (3) (2024) In press
   PUBMED   38408869
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 2884)
  AUTHORS   Li,R., Guan,Z., Bi,S., Wang,F., He,L., Niu,X., You,Y., Liu,Y.,
            Ding,Y., Siwko,S., Wang,N., Zhang,Z., Jin,Y. and Luo,J.
  TITLE     The proton-activated G protein-coupled receptor GPR4 regulates the
            development of osteoarthritis via modulating CXCL12/CXCR7 signaling
  JOURNAL   Cell Death Dis 13 (2), 152 (2022)
   PUBMED   35165253
  REMARK    GeneRIF: The proton-activated G protein-coupled receptor GPR4
            regulates the development of osteoarthritis via modulating
            CXCL12/CXCR7 signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2884)
  AUTHORS   Haque,M.E., Azam,S., Akther,M., Cho,D.Y., Kim,I.S. and Choi,D.K.
  TITLE     The Neuroprotective Effects of GPR4 Inhibition through the
            Attenuation of Caspase Mediated Apoptotic Cell Death in an MPTP
            Induced Mouse Model of Parkinson's Disease
  JOURNAL   Int J Mol Sci 22 (9), 4674 (2021)
   PUBMED   33925146
  REMARK    GeneRIF: The Neuroprotective Effects of GPR4 Inhibition through the
            Attenuation of Caspase Mediated Apoptotic Cell Death in an MPTP
            Induced Mouse Model of Parkinson's Disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2884)
  AUTHORS   Sato,K., Tobo,A., Mogi,C., Tobo,M., Yamane,N., Tosaka,M.,
            Tomura,H., Im,D.S. and Okajima,F.
  TITLE     The protective role of proton-sensing TDAG8 in the brain injury in
            a mouse ischemia reperfusion model
  JOURNAL   Sci Rep 10 (1), 17193 (2020)
   PUBMED   33057165
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2884)
  AUTHORS   Castellone,R.D., Leffler,N.R., Dong,L. and Yang,L.V.
  TITLE     Inhibition of tumor cell migration and metastasis by the
            proton-sensing GPR4 receptor
  JOURNAL   Cancer Lett 312 (2), 197-208 (2011)
   PUBMED   21917373
  REMARK    GeneRIF: findings suggest that GPR4 activation by an acidic pH
            inhibits tumor cell migration and invasion, and the Rho GTPase is
            at least partly responsible for this phenotype
REFERENCE   7  (bases 1 to 2884)
  AUTHORS   Sun,X., Yang,L.V., Tiegs,B.C., Arend,L.J., McGraw,D.W., Penn,R.B.
            and Petrovic,S.
  TITLE     Deletion of the pH sensor GPR4 decreases renal acid excretion
  JOURNAL   J Am Soc Nephrol 21 (10), 1745-1755 (2010)
   PUBMED   20798260
  REMARK    GeneRIF: pH sensor with an important role in regulating acid
            secretion in the kidney collecting duct
REFERENCE   8  (bases 1 to 2884)
  AUTHORS   Yang,L.V., Radu,C.G., Roy,M., Lee,S., McLaughlin,J., Teitell,M.A.,
            Iruela-Arispe,M.L. and Witte,O.N.
  TITLE     Vascular abnormalities in mice deficient for the G protein-coupled
            receptor GPR4 that functions as a pH sensor
  JOURNAL   Mol Cell Biol 27 (4), 1334-1347 (2007)
   PUBMED   17145776
  REMARK    GeneRIF: These results suggest that GPR4 deficiency leads to
            partially penetrant vascular abnormalities during development and
            that this receptor functions in blood vessel pH sensing.
REFERENCE   9  (bases 1 to 2884)
  AUTHORS   Ikeno,Y., Konno,N., Cheon,S.H., Bolchi,A., Ottonello,S.,
            Kitamoto,K. and Arioka,M.
  TITLE     Secretory phospholipases A2 induce neurite outgrowth in PC12 cells
            through lysophosphatidylcholine generation and activation of G2A
            receptor
  JOURNAL   J Biol Chem 280 (30), 28044-28052 (2005)
   PUBMED   15927955
REFERENCE   10 (bases 1 to 2884)
  AUTHORS   Vassilatis,D.K., Hohmann,J.G., Zeng,H., Li,F., Ranchalis,J.E.,
            Mortrud,M.T., Brown,A., Rodriguez,S.S., Weller,J.R., Wright,A.C.,
            Bergmann,J.E. and Gaitanaris,G.A.
  TITLE     The G protein-coupled receptor repertoires of human and mouse
  JOURNAL   Proc Natl Acad Sci U S A 100 (8), 4903-4908 (2003)
   PUBMED   12679517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148988.5.
            
            On Jul 30, 2009 this sequence version replaced NM_175668.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085829.1, SRR7652917.467821.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                AC148988.5         4493-4589
            98-2884             AC148988.5         13345-16131
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.46 cM"
     gene            1..2884
                     /gene="Gpr4"
                     /note="G protein-coupled receptor 4"
                     /db_xref="GeneID:319197"
                     /db_xref="MGI:MGI:2441992"
     exon            1..97
                     /gene="Gpr4"
                     /inference="alignment:Splign:2.1.0"
     exon            98..2884
                     /gene="Gpr4"
                     /inference="alignment:Splign:2.1.0"
     CDS             863..1960
                     /gene="Gpr4"
                     /codon_start=1
                     /product="G-protein coupled receptor 4"
                     /protein_id="NP_783599.1"
                     /db_xref="CCDS:CCDS20893.1"
                     /db_xref="GeneID:319197"
                     /db_xref="MGI:MGI:2441992"
                     /translation="MDNSTGTGEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAA
                     YRQVRQHNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSN
                     IYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDEL
                     FRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAVQSSVSTER
                     QEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAF
                     TSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRS
                     TTGKSSGAVWAVPPTAQGDQVPLKVLLPPAQ"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr4"
                     /note="hexamer: AAGAAA"
     polyA_site      2047
                     /gene="Gpr4"
                     /note="major polyA site"
     regulatory      2859..2864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr4"
                     /note="hexamer: AATAAA"
     polyA_site      2877
                     /gene="Gpr4"
ORIGIN      
        1 gctgtcctgc cccgcggggg cgcccgccgg gtccctgtgc tgtgcagagt cgggaccaag
       61 tcagagacgc cggtcgccca gcccagccgc ggagcaggcc tagcaatctc agctctcaat
      121 gcagtcgtga gtggaacaac ttcatctcca ctcctcagtc tccaccagga tcaactctga
      181 gtctcctgga acccccttca gagtccccct cctacacaca atcctgtgct ccaccactta
      241 ctcaccttcc tgtttcctag catcccttcc cacagtcacc agcctttaca agtccttcct
      301 ctttgtctgt ccccagagga cacctcattt tttttcctgc tttggtgact ttacatagtt
      361 ctctttccag ctggtgtcct agcagatgcc agcactctta aaattgcatc ttgccaactc
      421 tgtgacttct ctccatgctc ccatgtcttt ccttagtctt ctagaacatt ccatcccatt
      481 ggtacttcac catgtccata ttgacttcct aagatttcaa taagcatgac accgctggtg
      541 tcttcatctc ctctgtaaac actgccttga cctttttttt tctggtgcca tctttgtgct
      601 ctggttgtgt agatagccca ttgacattag ttccacctct tatccttacc ttcatcatcc
      661 cttgacactc ttcctctcaa gtcctccctt tgcttagcct gccacaaagc aaacccagac
      721 ctcagtcctc gtgaactgtc tcctgtgatg ttcccctgag cctccaaaga gggggctcag
      781 ggggcctgat ggcctcccgc cccccgtggc cctgtagccc cagcgggcca ggggaagctc
      841 ctgggaagcc ccatcgccca ggatggacaa cagcacgggc acaggggagg gctgccatgt
      901 ggactctcga gtggaccacc tcttcccacc atctctctac atcttcgtca tcggggtggg
      961 gctgcccacc aactgcctgg ccctgtgggc agcctaccgg caggtgcgcc aacacaatga
     1021 gctgggcgtc tacctgatga acttgagcat tgcagacctg ctgtacatct gcactttgcc
     1081 gctgtgggtc gactacttcc tccaccatga caactggatc cacggccctg gctcctgcaa
     1141 gctctttggc ttcatcttct acagcaacat ctatatcagc atcgccttcc tgtgctgcat
     1201 ctccgtggac cgctacctgg ctgtggctca tcctctgcgc tttgcacgcc tgcgccgggt
     1261 caagacagca gtggctgtga gctctgtggt ctgggccacg gagctgggcg ccaattcagc
     1321 accgctcttc catgatgagc tgtttcgtga tcgctacaac cacaccttct gctttgagaa
     1381 gttccccatg gagcgttggg tggcctggat gaatctgtac cgcgtctttg tgggcttcct
     1441 cttcccctgg gcactcatgt tgctgtgcta ccgtggcatc ctgagggcag tgcagagcag
     1501 tgtgtccacc gagcgccagg agaaagtcaa gatcaaacgt ctggccctga gcctcatcgc
     1561 cattgtgctg gtgtgctttg cgccttacca tgctctcctg ctgtctcgca gcgccgtcta
     1621 cctgggccgg ccctgggact gtggcttcga ggagcgagtc ttttctgcct accacagctc
     1681 cctggccttc accagcctca attgtgtggc tgaccccatc ctctactgcc tggtcaacga
     1741 gggtgcccgc agtgatgtgg ccaaggccct gcacaacctc ctccgcttcc tggccagcaa
     1801 caagccccag gagatggcca atgcttccct caccctggag acacccttga cctccaagag
     1861 gagcaccacc ggcaagtcgt ccggggctgt ctgggcagtg cctccgactg cccaggggga
     1921 ccaggtgcca ctgaaggtgc tgctgccccc ggcacagtga gccccatgcc caactgtcat
     1981 cctgcaccct tccggttgta tgcaaatgtg tgtaaatatg tccatgtgaa ttacaagaaa
     2041 aaaatgaggt tttttttttc tctctgtcat cttcttccac tatgatcccc aatgattcac
     2101 ttaatcccag cagggtctga agggggaaca acacagccag gagtttgtca tcatgggcgc
     2161 ccagatgggg agccagccat ggagagtttg ggatgagggg ataattaagc caactgcttg
     2221 gtttctggca gataaggaca gacgagtctg atcagatgct acaagtgtgt gattccagct
     2281 acttgggagg ccgaggaaag atttggaagt tcaagaccca cttggaccac aaagtgaagc
     2341 cctgtctcaa aatattaaat acaacaaagc ccggggatgt aatctagcag tttttatcta
     2401 aaattcccag tgaggggctt ggggcgtggc ccaagtggga gagcccctgc ctagaatcca
     2461 ttctggtaat acctagcatg ctggtaattc cttcttgttt gtattttgga aagctattta
     2521 ccacagtctg gctttgaggt tatggtcttc cttcctcagc ttcccaagtg ctaggcttac
     2581 agatgttaac tgctatgtca agcagatatt ctgagattct acacaaacag cattctctca
     2641 gtctcagatg gtgaaggagg acagggactg tcaagagcag gtaagagcca atctgagcag
     2701 cctgcctgaa ggaatcgtaa gcagggctgg tttagaaagg aggcccagga caggaagagc
     2761 atccattcat gcactgtcta atcacatctt gcctcagaga ttaggttggt ctgtgaagac
     2821 cagatcctgc tatcagggag gtaggactaa caataaaaaa taaacatagc aagacaattc
     2881 cctt
//
