LOCUS       NM_001359056            2471 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus G-protein coupled receptor 12 (Gpr12), transcript
            variant 4, mRNA.
ACCESSION   NM_001359056
VERSION     NM_001359056.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Hsiao,K., Noble,C., Pitman,W., Yadav,N., Kumar,S., Keele,G.R.,
            Terceros,A., Kanke,M., Conniff,T., Cheleuitte-Nieves,C.,
            Tolwani,R., Sethupathy,P. and Rajasethupathy,P.
  TITLE     A Thalamic Orphan Receptor Drives Variability in Short-Term Memory
  JOURNAL   Cell 183 (2), 522-536 (2020)
   PUBMED   32997977
  REMARK    GeneRIF: A Thalamic Orphan Receptor Drives Variability in
            Short-Term Memory.
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Frank,E., Wu,Y., Piyaratna,N., Body,W.J., Snikeris,P., South,T.,
            Gerdin,A.K., Bjursell,M., Bohlooly-Y,M., Storlien,L. and Huang,X.F.
  TITLE     Metabolic parameters and emotionality are little affected in
            G-protein coupled receptor 12 (Gpr12) mutant mice
  JOURNAL   PLoS One 7 (8), e42395 (2012)
   PUBMED   22879962
  REMARK    GeneRIF: Studying metabolic and emotionality parameters in Gpr12
            mutant mice did not reveal a major phenotypic impact of the gene
            mutation.
REFERENCE   3  (bases 1 to 2471)
  AUTHORS   Tanaka,S., Ishii,K., Kasai,K., Yoon,S.O. and Saeki,Y.
  TITLE     Neural expression of G protein-coupled receptors GPR3, GPR6, and
            GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth
  JOURNAL   J Biol Chem 282 (14), 10506-10515 (2007)
   PUBMED   17284443
REFERENCE   4  (bases 1 to 2471)
  AUTHORS   Bjursell,M., Gerdin,A.K., Jonsson,M., Surve,V.V., Svensson,L.,
            Huang,X.F., Tornell,J. and Bohlooly-Y,M.
  TITLE     G protein-coupled receptor 12 deficiency results in dyslipidemia
            and obesity in mice
  JOURNAL   Biochem Biophys Res Commun 348 (2), 359-366 (2006)
   PUBMED   16887097
  REMARK    GeneRIF: GPCR12 is considered important for the energy balance
            since GPCR12 KO mice develop obesity and have lower energy
            expenditure.
REFERENCE   5  (bases 1 to 2471)
  AUTHORS   Hinckley,M., Vaccari,S., Horner,K., Chen,R. and Conti,M.
  TITLE     The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP
            signaling and maintenance of meiotic arrest in rodent oocytes
  JOURNAL   Dev Biol 287 (2), 249-261 (2005)
   PUBMED   16229830
  REMARK    GeneRIF: the cAMP levels required for maintaining meiotic arrest in
            mouse and rat oocytes are dependent on the expression of Gpr3
            and/or Gpr12
REFERENCE   6  (bases 1 to 2471)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   7  (bases 1 to 2471)
  AUTHORS   Ignatov,A., Lintzel,J., Hermans-Borgmeyer,I., Kreienkamp,H.J.,
            Joost,P., Thomsen,S., Methner,A. and Schaller,H.C.
  TITLE     Role of the G-protein-coupled receptor GPR12 as high-affinity
            receptor for sphingosylphosphorylcholine and its expression and
            function in brain development
  JOURNAL   J Neurosci 23 (3), 907-914 (2003)
   PUBMED   12574419
REFERENCE   8  (bases 1 to 2471)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   9  (bases 1 to 2471)
  AUTHORS   Saeki,Y., Ueno,S., Mizuno,R., Nishimura,T., Fujimura,H., Nagai,Y.
            and Yanagihara,T.
  TITLE     Molecular cloning of a novel putative G protein-coupled receptor
            (GPCR21) which is expressed predominantly in mouse central nervous
            system
  JOURNAL   FEBS Lett 336 (2), 317-322 (1993)
   PUBMED   8262253
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127589.4.
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 3. Variants 1-5 all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.5134332.1,
                                           SRR9219382.202216.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-495               AC127589.4         6209-6703           c
            496-2471            AC127589.4         3614-5589           c
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 85.23 cM"
     gene            1..2471
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="G-protein coupled receptor 12"
                     /db_xref="GeneID:14738"
                     /db_xref="MGI:MGI:101909"
     exon            1..495
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /inference="alignment:Splign:2.1.0"
     exon            496..2471
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /inference="alignment:Splign:2.1.0"
     CDS             511..1515
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="isoform a is encoded by transcript variant 4"
                     /codon_start=1
                     /product="G-protein coupled receptor 12 isoform a"
                     /protein_id="NP_001345985.1"
                     /db_xref="CCDS:CCDS19871.1"
                     /db_xref="GeneID:14738"
                     /db_xref="MGI:MGI:101909"
                     /translation="MNEDPKVNLSGLPRDCIDAGAPENISAAVPSQGSVAESEPELVV
                     NPWDIVLCSSGTLICCENAVVVLIIFHSPSLRAPMFLLIGSLALADLLAGLGLIINFV
                     FAYLLQSEATKLVTIGLIVASFSASVCSLLAITVDRYLSLYYALTYHSERTVTFTYVM
                     LVMLWGTSICLGLLPVMGWNCLRDESTCSVVRPLTKNNAAILSISFLFMFALMLQLYI
                     QICKIVMRHAHQIALQHHFLATSHYVTTRKGVSTLALILGTFAACWMPFTLYSLIADY
                     TYPSIYTYATLLPATYNSIINPVIYAFRNQEIQKALCLICCGCIPSSLSQRARSPSDV
                     "
     regulatory      1849..1854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="hexamer: AATAAA"
     polyA_site      1872
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="major polyA site"
     regulatory      2199..2204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="hexamer: ATTAAA"
     polyA_site      2223
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
     regulatory      2417..2422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
                     /note="hexamer: AATAAA"
     polyA_site      2447
                     /gene="Gpr12"
                     /gene_synonym="Gpcr01; Gpcr12; Gpcr20; Gpcr21"
ORIGIN      
        1 aagggaacaa taatttgcag accggccaac tgcaatctaa gagagggagt cgcttgctgt
       61 tgtaagtctc ctccgccagc cctaacctgc ttaccccgca ttcctcctgt tcatcccgaa
      121 aacccggccg tttacaattc tttaggggaa agcataagaa gccgagcccc agggtcaagg
      181 gcgcctcggg gaagccacag gatcaaagta ggtcgccaga ctctccggcc gttcgagtgg
      241 gtcttcgcat gactgttgca ggcgggcgtc cacggtggcg ggctcccgcc cctcacgcag
      301 ctgcgacctg cgggggcgcg cgcagcctcg tggggttccc gcggatgcgc gcccggcggg
      361 gagcgcggag ggcggagagc cgggcgcgag caccgcagct cacctgccgc gggcgccacc
      421 acggacgtgc cacgcgggtg gcccgagcta ttcggcagca ctgaaggagc cacccctcgg
      481 ccagggcgtg ccaagaggac aggggttaaa atgaacgaag acccgaaggt caatttaagc
      541 gggctgcctc gggactgtat agatgccggt gctccagaga acatctcagc cgctgtcccc
      601 tcccagggct ctgttgcgga gtcagaaccc gagctcgttg tcaacccctg ggacattgtc
      661 ttgtgcagct caggaaccct catctgctgt gaaaatgccg ttgtggtcct tatcatcttc
      721 cacagcccca gcctgcgagc ccccatgttc ctactgatag gcagcctggc tcttgcagac
      781 ctgctggctg gcctgggact catcatcaat tttgtttttg cgtacctgct tcagtcagaa
      841 gccaccaagc tggtcaccat cggactcatt gtcgcctctt tctctgcctc tgtctgcagt
      901 ttgctggcta ttactgtgga ccgctacctc tcgctatatt acgccctgac gtaccactcc
      961 gagaggaccg tcacctttac ctatgtcatg ctagtgatgc tctggggaac ctccatctgc
     1021 ctggggctgc tgcccgtcat gggctggaac tgcttgaggg acgagtccac ctgcagcgtg
     1081 gtcagacctc tcactaagaa caacgctgcc atcctctcca tctccttcct cttcatgttt
     1141 gctctgatgc ttcagctcta catccagatt tgtaagattg tgatgaggca cgcccatcag
     1201 atagccctgc agcaccactt cctggctaca tcgcactatg tgactacccg gaaaggggtc
     1261 tcgaccctgg ctctcatcct agggaccttt gctgcctgct ggatgccttt caccctctat
     1321 tccttgatcg ccgattacac ctacccttcg atctatacct atgccaccct cctgcccgcc
     1381 acctacaatt ccatcatcaa ccctgtcatt tacgctttca gaaaccaaga gatccagaaa
     1441 gccctctgcc tcatttgctg tgggtgcatc ccttcctcgc tgtctcagag agctcggtct
     1501 cccagcgatg tgtagcagcc ttctcctcat aggacgctgc ctctaccaag cgctcccacc
     1561 tcccagggcg gccagtgatt tccttcctta aattctttgc actggatctc acaagcagaa
     1621 gcaatgacat cttttagaca cgtattgaca gtggaaatca tcttaccagt gttttttaaa
     1681 aaaaaaacaa aacaaaactc gacttctcgg ctcagcattc tgttgtttgg tttgggagtt
     1741 aggatttgtt tgtttgtttg cttgtttgtt tgtttggagg gtgtaatggg acctcatgtg
     1801 gccatgaaat tatacaaaag tctcgggatt ttttaaccta ggcttgaaaa taaatcaaag
     1861 ttttaaagga aactggagaa ggaaatactt tttctgaagg aaatactttt ttttttttaa
     1921 tcaaggtaga tcttccattc tgtatgtatc taacaggata ggagctttgc catataacca
     1981 aaatagttta tataattaca tttggaaggg cttgtgttta tttctaggaa ttcagtaata
     2041 agtgaccagt aacagaggcg cgaactcctt tctttccttt cagcagtagt gactgctctt
     2101 aagaatcact ttgcagtttc tctgtgttac agtttggtat gcatggttac ctgtggtagt
     2161 cagatcacta attgcaatat tgccatgtta aacccagaat taaaagagtc attttttctt
     2221 caatacagtt tttgaaatat cctttccaaa gtgagtcatg aaaaaaatgt ttccaattac
     2281 atatgagata gcactggtta gatttgtcat tgtgattttt aaaactctag actggtggtt
     2341 ttcagaaaac aaaagagaaa atattaacag catctattga aagaagattt tatttatttt
     2401 taatatattc tgagagaata aatggtgtga tactattaag aaatatacaa acatgacttt
     2461 tcaaatctct a
//
