LOCUS       NM_011821               6750 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Mus musculus glypican 6 (Gpc6), transcript variant 2, mRNA.
ACCESSION   NM_011821
VERSION     NM_011821.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Mead,T.J., Bhutada,S., Foulcer,S.J., Peruzzi,N., Nelson,C.M.,
            Seifert,D.E., Larkin,J., Tran-Lundmark,K., Filmus,J. and Apte,S.S.
  TITLE     Combined genetic-pharmacologic inactivation of tightly linked
            ADAMTS proteases in temporally specific windows uncovers distinct
            roles for versican proteolysis and glypican-6 in cardiac
            development
  JOURNAL   Matrix Biol 131, 1-16 (2024)
   PUBMED   38750698
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6750)
  AUTHORS   Wishart,T.F.L. and Lovicu,F.J.
  TITLE     Spatiotemporal Localisation of Heparan Sulphate Proteoglycans
            throughout Mouse Lens Morphogenesis
  JOURNAL   Cells 12 (10), 1364 (2023)
   PUBMED   37408198
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6750)
  AUTHORS   Shi,W. and Filmus,J.
  TITLE     Glypican-6 and Glypican-4 stimulate embryonic stomach growth by
            regulating Hedgehog and noncanonical Wnt signaling
  JOURNAL   Dev Dyn 251 (12), 2015-2028 (2022)
   PUBMED   36057966
  REMARK    GeneRIF: Glypican-6 and Glypican-4 stimulate embryonic stomach
            growth by regulating Hedgehog and noncanonical Wnt signaling.
REFERENCE   5  (bases 1 to 6750)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6750)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   7  (bases 1 to 6750)
  AUTHORS   Takasato,M., Osafune,K., Matsumoto,Y., Kataoka,Y., Yoshida,N.,
            Meguro,H., Aburatani,H., Asashima,M. and Nishinakamura,R.
  TITLE     Identification of kidney mesenchymal genes by a combination of
            microarray analysis and Sall1-GFP knockin mice
  JOURNAL   Mech Dev 121 (6), 547-557 (2004)
   PUBMED   15172686
REFERENCE   8  (bases 1 to 6750)
  AUTHORS   Powles,N., Babbs,C., Ficker,M., Schimmang,T. and Maconochie,M.
  TITLE     Identification and analysis of genes from the mouse otic vesicle
            and their association with developmental subprocesses through in
            situ hybridization
  JOURNAL   Dev Biol 268 (1), 24-38 (2004)
   PUBMED   15031102
REFERENCE   9  (bases 1 to 6750)
  AUTHORS   Ford-Perriss,M., Turner,K., Guimond,S., Apedaile,A., Haubeck,H.D.,
            Turnbull,J. and Murphy,M.
  TITLE     Localisation of specific heparan sulfate proteoglycans during the
            proliferative phase of brain development
  JOURNAL   Dev Dyn 227 (2), 170-184 (2003)
   PUBMED   12761845
REFERENCE   10 (bases 1 to 6750)
  AUTHORS   Veugelers,M., De Cat,B., Ceulemans,H., Bruystens,A.M., Coomans,C.,
            Durr,J., Vermeesch,J., Marynen,P. and David,G.
  TITLE     Glypican-6, a new member of the glypican family of cell surface
            heparan sulfate proteoglycans
  JOURNAL   J Biol Chem 274 (38), 26968-26977 (1999)
   PUBMED   10480909
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164668.1, AK135442.1, AK132531.1 and AC132136.3.
            
            On Oct 22, 2013 this sequence version replaced NM_011821.2.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            central coding region, compared to variant 1. The encoded isoform
            (2) is shorter, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK164668.1, AK132531.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               AK164668.1         2-138
            138-265             AK135442.1         120-247
            266-2622            AK132531.1         3-2359
            2623-6750           AC132136.3         188183-192310
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 60.34 cM"
     gene            1..6750
                     /gene="Gpc6"
                     /note="glypican 6"
                     /db_xref="GeneID:23888"
                     /db_xref="MGI:MGI:1346322"
     exon            1..799
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     CDS             640..2307
                     /gene="Gpc6"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="glypican-6 isoform 2 precursor"
                     /protein_id="NP_035951.1"
                     /db_xref="CCDS:CCDS27330.1"
                     /db_xref="GeneID:23888"
                     /db_xref="MGI:MGI:1346322"
                     /translation="MPSWIRAVILPLSGLLLTLPAAADVKARSCSEVRQAYGAKGFSL
                     ADIPYQEIAGEHLRICPQEYTCCTTEMEDKLSQQSKLEFENLVEETSHFVRTTFVSRH
                     KKFDEFFRELLENAEKSLNDMFVRTYGMLYMQNSEVFQDLFTELKRYYTGGNVNLEEM
                     LNDFWARLLERMFQLINPQYHFSEDYLECVSKYTDQLKPFGDVPRKLKIQVTRAFIAA
                     RTFVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYCLNVMKG
                     CLANQADLDTEWNLFIDAMLLVAERLEGPFNIESVMDPIDVKISEAIMNMQENSMQVS
                     AKVFQGCGQPKPAPALRSARSAPENFNTRFRPYNPEERPTTAAGTSLDRLVTDIKEKL
                     KLSKKVWSALPYTICKDERVTAGTSNEEECWNGHSKARYLPEIMNDGLTNQINNPEVE
                     VDITRPDTFIRQQIMALRVMTNKLKNAYNGNDVNFQDTSDESSGSGSGSGCMDDVCPT
                     EFEFVTTEAPAVDPDRREEESSASKFSSSLISWSLVCMVLALQRLYR"
     exon            800..958
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1350
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1516
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1647
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1791
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1928
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2104
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..6750
                     /gene="Gpc6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2605..2610
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpc6"
                     /note="hexamer: ATTAAA"
     polyA_site      2627
                     /gene="Gpc6"
     regulatory      6727..6732
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpc6"
                     /note="hexamer: ATTAAA"
     polyA_site      6750
                     /gene="Gpc6"
                     /note="major polyA site"
ORIGIN      
        1 agtctccagg attttctctc ttcccatttc aagaagactc tcactggctc cctcagtcta
       61 tgtgaagctg aggtttcccc tcgatcccgt acatctccat cacatacctc cacgcacaca
      121 cctccccgag agccccgcgt tcacacccac aaacacgcgc gctcacactc gcgccggcct
      181 gtccatctcc ctcggggtgg ggggagccgg cgcgcggtcc caccttcgtc gcgcacactg
      241 gataggcgag ctcgcagcgc tccacgactc tgcggctcgg aactcggacc gcagggctga
      301 acacccctac tgtggtgttt aaaaaaaaaa aaagacattt ctttgccttt tcctcttttc
      361 tcctctttct ccaacgattt tctaccggag tggctagcgg agccggcagc cttcccaagg
      421 cagccctggt tggcttgcca tcctccatct ggcttataaa agtttgctga gtgcagtcca
      481 gaggtctgcg cggctcgtcc cctcggctgg cggaaggggg tgacgctggg cagcggctaa
      541 ggagcgcgcc gcaggctctg gcgggctttc ggctcgaggg gcaaggtgaa gagcgcacag
      601 ggagctgggg gctgccgagg tggacttgca tgttgcacca tgccttcttg gatccgggct
      661 gtgattcttc ctctctccgg gctgctgctg accctcccgg ccgcggcgga cgtgaaggct
      721 cggagctgca gcgaggtccg tcaggcttac ggtgccaagg gattcagcct ggcggacatc
      781 ccctaccagg agatcgcagg ggagcactta cgcatctgcc ctcaggaata tacctgctgc
      841 accacagaaa tggaagacaa gctgagtcaa cagagtaaac tggagtttga aaaccttgta
      901 gaagagacaa gccactttgt gaggaccacg tttgtgtcga ggcacaagaa atttgatgag
      961 tttttccgag agctgctgga aaacgcagaa aagtccctaa atgacatgtt tgtccggacc
     1021 tacgggatgc tgtacatgca gaattcagag gtattccagg acctcttcac tgagctaaag
     1081 cggtactaca cagggggtaa cgtcaacctg gaagagatgc tcaatgactt ctgggctcgg
     1141 ctcctggaga ggatgttcca gctgattaac cctcagtatc acttcagcga ggactacctg
     1201 gagtgtgtaa gcaagtacac agaccagctg aagccatttg gagacgtgcc acggaaactg
     1261 aagattcagg tcacccgcgc cttcatcgcg gctcgcacct ttgtccaggg cctgacagtg
     1321 ggcagagagg ttgccaaccg agtttccaag gtgagcccca cccctggctg catccgggct
     1381 ctcatgaaga tgctgtactg cccatactgc cgaggcttgc ccactgtgag accttgcaac
     1441 aactactgcc tcaacgtcat gaagggctgc ctggccaatc aggcagattt ggacactgag
     1501 tggaatctct tcatagatgc aatgctcttg gtggctgagc gactggaagg gccattcaac
     1561 attgagtcag tcatggaccc aatagacgtc aagatctctg aagccattat gaacatgcag
     1621 gaaaacagca tgcaggtgtc agcaaaggtc tttcagggct gtggccagcc caaacctgct
     1681 cccgctctca gatctgctcg ctcagctcca gaaaatttta acacccgttt tcggccctac
     1741 aaccctgagg aaagacccac aactgcagcg ggcacgagct tggaccggct ggtcacagac
     1801 ataaaagaga agctgaagct ctctaaaaag gtctggtcag cattgcccta caccatctgc
     1861 aaggacgaac gtgtgacagc aggaacctcc aacgaggagg agtgctggaa cggacacagc
     1921 aaagccagat acctgcctga gatcatgaac gatgggctga ccaaccaaat caacaatcct
     1981 gaggtggagg tggacatcac gcggccagac actttcatca gacagcagat catggctctc
     2041 cgtgtgatga ccaacaaact gaagaatgcg tacaatggca atgacgtcaa cttccaagac
     2101 acaagcgatg aatccagtgg ctcaggcagc ggcagtggtt gcatggatga tgtgtgtccc
     2161 acggagttcg agtttgtcac caccgaggcc ccggcggtgg acccggaccg cagagaagag
     2221 gaatcttcgg cctcgaagtt cagctcctcc ttgatttcct ggtctctggt ctgcatggtc
     2281 ttggccctgc agagactcta cagataatcg tgggctttgg tcagacgaaa ctgcgtttta
     2341 gctcttctgt ggccaacacc cttcttttct tacactcttg aacaatggac catgccacac
     2401 aacaagccgt tttctatgac aagagagcag tcttgaggtc tgcctcccct ttttgctttc
     2461 ccaaagagta ccgggtgcca gatggcttgc ttctctttcc gtcagctatc tgtggggacc
     2521 tcgtttattc cagagagaat tcttactcaa acctttcgta ccaagagatt gttttcttac
     2581 cgtcgtttgc ttttatgctg caaaattaaa agaatttcat gttgtgaggg gggttttggt
     2641 tttgtttttt tttacaaccc atttaaaata ggaaaaaata attttccgtt tcaaatcagg
     2701 ccaaacccca agacaactgc attctcaaca aggaaacaaa caaaggagag aaaataaagg
     2761 aactttcatg ctatcaggct tggcactgac agccaactcc tggagtgagc ttttctgtga
     2821 caagcgaggt ttacaaagcg cttatgagga gaaagcgtaa cattttcatg tagtggaaat
     2881 acaccgttgt cttggaatct aaccaaactg atttgaaggt actgatagta aatttaggac
     2941 tctgtcttta tatgaacggt aatcttatga caactcaatg tctctcttcc cactgcctga
     3001 tcccaccgct cactgatggc agcattttct gtctcctaac gtctgtccgt tttatccaat
     3061 tgacactgtc ttcagttttt catagatgcc ccgcccccat ctagtctcct ctctcctagg
     3121 tcttgactca aaagtcatct tcagtggtaa caatctcttc tttctccaga cggacatgaa
     3181 ccagatgtct cagctcctgt gttccggacc ctcccccaaa tacgagtttt cttactgaca
     3241 tgacaattgg ctccacacat gaggttctgt tactgactct ttgtgtcaat tggtctcagc
     3301 atcacaaggg cattctctta acataacaac tcacaacaaa acaagtcaag acaaaaagag
     3361 agagaggata aaacattata tatagtattg acacggaggt tcttttcact tttttagtct
     3421 tagtatgatc atctattttt atatctatat tatatatata tataaatcac agattttaac
     3481 ttcttaattc caagctcagg gttgacactc ctttgtaaca gaactgtttt gtcaaccgcc
     3541 ccgtcttgtt ctgtgctgct cagagaacag actccttagt gatcttcgag caccaagaat
     3601 caagagcgag aactttttat gtatctaact gtccattgat ggcaagtttg ccagggcaca
     3661 cccttctcac acacacttgc ttttcccctg gtgctccaaa ctgtcctgag ctcatgggac
     3721 aagcctggtg tgcaagacgt gactggtgat ggaatgtgtg tgtgtaaaga atgttattca
     3781 tgaaaaaaac aaaaaacaaa aaaacattgc tctctggtac agcacctctg gccttggact
     3841 tctaccgggt actgtactga atggcctaga ctcaagaggc cattttagag ccactgccta
     3901 acctgtccac caagccaggc ccaatgcttc ctctgacaga agcgaaatac accaaactgg
     3961 ggttgtatga agacagttgg ctaaccacct tctcctcctc ctcctccttc ttctttcttc
     4021 ttcttcttct tcttcttctt cttcttcttc ttcttcttct tcttcttctt cttcttcttc
     4081 tgcttcttct gcttcttctg cttcttctgc ttcttctgct tcttctgctt cttctccttc
     4141 tccttctcct tctccttctc cttctccttc tccttctcct tctccttctc cttctccttc
     4201 tccttctcct tcttcttctt cttcttcttc ttcttcttct tctccttctc cttctttctc
     4261 ctccttctcc ttctccttct ccttctcctt cttctgcttt tatgatgcat ctgggatagg
     4321 aaaaagaaat atctcaagat accacagcat gccaatcaca tttttagctg actcatcgac
     4381 agtattgaca cataaatgat atttttctat ctctgttctc accaacatga aaattagtta
     4441 ccgatttagt catcacgtct aagtagaaca cacgcactca tattaagtat atttgagcac
     4501 ttttgaaaga cagaacgtat ttacaagcat ctgttaccac cttatgccta acagaataag
     4561 caatgtagga ttctgagaga ctctgtggcg ttttttcatt tacatttgtg tgacattaac
     4621 actttatcca actcagaacg gtatcctttt gggaaaccac ccagctcaga accccacttt
     4681 ggcttctaat cccccgttac acataacact tctctatgaa ggaccagcca cttatgttag
     4741 gtggctcact gtatgttcag aaatcagtat gtttttttcc aataatcatg gtgcaatagg
     4801 aattaaacca atgtggattc tccaggattt ttgagaataa ggacttattt ttacggcttt
     4861 aagctccaac ttccttttaa aatatttctg cattatcttc tataaaacaa atcaagatat
     4921 cacattcctt actatgcttc tgcccccaga tggtttacat gacaacacat tacatgaatg
     4981 aatgtgagcc catgtcctgt gcacactcat cacacaaata atatagaaat gcctgtttcc
     5041 tttgactcag cacctgtatt ctcaagggta cgactactga taagatacag tgatgcccat
     5101 gagccaggca gagctgagat gagggtcagc tgacaaagcc acatcgttgg tcatcttaca
     5161 cagacatgga caaaatgttt ttaacgtgcc cttcctagtc attgtcactg cccgttgtat
     5221 ggttttgtag actgatttaa tgttaaaggt ttaatttttt tttcctgtcc cctaagcctt
     5281 ttcgttcagt ttcccctttt cggtactttg ggatcctcct cccagcttga gtttcccatt
     5341 ccagcacagt gataggagat gtctcaatgt tcccttgtca cccacagcct accctcattt
     5401 gtgacgatgt tgtcccctaa aatctgtaat cacatcacga cttgtgttac gagtagagag
     5461 gaacggaacc ccaaaattgg acactagaaa ccggtgggtg atgctcatga ctacaaacac
     5521 ttagcttatc caagtgcctc tatttacatg ttccttaggt ttaatatgta tttttctaac
     5581 agaaataccc gtctgcaatt gatgtttatt atactttgta tatgttacaa caaaagctaa
     5641 acggaggcta atgtcttcag cagagaagaa tgaactgtaa atttatgaac acgaacgctg
     5701 tagtcctgta ttggcagcca caagacagct ttctgtctgt cactgcagtg aaggccaagt
     5761 tggcactttg attacagcca tccccgacta tagcactaga attctgactt ccttactggg
     5821 atttacccaa ggaggaaggt gacgtgactc atctgcttca aacataaagg agagaaacta
     5881 ctttatctac accgagaaac tctatccact gaggcacaac agtagtttca aatggagcat
     5941 tctttaaatc aaacacattg aagatattat tggatgtaaa accatgaaac ttaatttaca
     6001 ttccctgcta tagacattag ccttctttaa ttcttcttgc cattatatac atattttggc
     6061 tccagactaa gcaccaactt aagtcttcaa ggaggaataa aacttagcaa gattctggct
     6121 tgcaaatctt ttctcacctc tctgcaaggc tgagtaccag gtggtttgac tttcggtttc
     6181 aaatgttggc aaatgcagct tttgacttct ccaaaagctt tcagatactc aacctcattt
     6241 tctttgactt atagtcacta gcagatattt aaattaaaag aatatgtagc cttgctgcca
     6301 gaagagcaaa ggagtccata acttataaat ctgcagtgtg ttcctccttg gtagtgatat
     6361 tggccagtgc ttagttttag gccaacatat acaaatagag tattgatttg tacgcattct
     6421 ggagacttgc tatgtcatag tttgctatag cccagactca gcttagcatg ggaatgcttg
     6481 ggacttcact gagagtggag tggctgctgc aatctttggc attgccttgt aattagggac
     6541 ttcatgagca acaccttaac gagggtacca cagtgcggtc tctgtgtgca tatacagaac
     6601 tcatgtgcag gataaatgtg tgcaccaagc aaacctgaaa ctttatgtga ctgtcaaatg
     6661 actattattt gggttaagat ttttttttca tttctgattt ggatagaaaa ttgctattaa
     6721 tcttgtatta aaatagtttt taaaaatcta
//
