LOCUS       NM_175500               2885 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus glypican 5 (Gpc5), mRNA.
ACCESSION   NM_175500
VERSION     NM_175500.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wishart,T.F.L. and Lovicu,F.J.
  TITLE     Spatiotemporal Localisation of Heparan Sulphate Proteoglycans
            throughout Mouse Lens Morphogenesis
  JOURNAL   Cells 12 (10), 1364 (2023)
   PUBMED   37408198
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2885)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   4  (bases 1 to 2885)
  AUTHORS   Wu,J., Li,H., Han,L., Sun,T., Tian,Y. and Wang,X.
  TITLE     The spatiotemporal expression pattern of Syndecans in murine
            embryonic teeth
  JOURNAL   Gene Expr Patterns 36, 119109 (2020)
   PUBMED   32220631
REFERENCE   5  (bases 1 to 2885)
  AUTHORS   Guo,W. and Roelink,H.
  TITLE     Loss of the Heparan Sulfate Proteoglycan Glypican5 Facilitates
            Long-Range Sonic Hedgehog Signaling
  JOURNAL   Stem Cells 37 (7), 899-909 (2019)
   PUBMED   30977233
  REMARK    GeneRIF: Using mouse embryonic stem cell-derived mosaic tissues
            with compartments that lack Exostosin1 and Exostosin2, or
            Glypican5, it has been shown that Shh accumulates around its source
            cells when they are surrounded by cells that have a mutated
            extracellular matrix.
REFERENCE   6  (bases 1 to 2885)
  AUTHORS   de Pontual,L., Yao,E., Callier,P., Faivre,L., Drouin,V., Cariou,S.,
            Van Haeringen,A., Genevieve,D., Goldenberg,A., Oufadem,M.,
            Manouvrier,S., Munnich,A., Vidigal,J.A., Vekemans,M., Lyonnet,S.,
            Henrion-Caude,A., Ventura,A. and Amiel,J.
  TITLE     Germline deletion of the miR-17 approximately 92 cluster causes
            skeletal and growth defects in humans
  JOURNAL   Nat Genet 43 (10), 1026-1030 (2011)
   PUBMED   21892160
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2885)
  AUTHORS   Luxardi,G., Galli,A., Forlani,S., Lawson,K., Maina,F. and Dono,R.
  TITLE     Glypicans are differentially expressed during patterning and
            neurogenesis of early mouse brain
  JOURNAL   Biochem Biophys Res Commun 352 (1), 55-60 (2007)
   PUBMED   17107664
REFERENCE   8  (bases 1 to 2885)
  AUTHORS   Ford-Perriss,M., Turner,K., Guimond,S., Apedaile,A., Haubeck,H.D.,
            Turnbull,J. and Murphy,M.
  TITLE     Localisation of specific heparan sulfate proteoglycans during the
            proliferative phase of brain development
  JOURNAL   Dev Dyn 227 (2), 170-184 (2003)
   PUBMED   12761845
REFERENCE   9  (bases 1 to 2885)
  AUTHORS   Litwack,E.D., Ivins,J.K., Kumbasar,A., Paine-Saunders,S.,
            Stipp,C.S. and Lander,A.D.
  TITLE     Expression of the heparan sulfate proteoglycan glypican-1 in the
            developing rodent
  JOURNAL   Dev Dyn 211 (1), 72-87 (1998)
   PUBMED   9438425
REFERENCE   10 (bases 1 to 2885)
  AUTHORS   Saunders,S., Paine-Saunders,S. and Lander,A.D.
  TITLE     Expression of the cell surface proteoglycan glypican-5 is
            developmentally regulated in kidney, limb, and brain
  JOURNAL   Dev Biol 190 (1), 78-93 (1997)
   PUBMED   9331333
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK134807.1, AK038547.1 and CJ237840.1.
            
            On Dec 3, 2009 this sequence version replaced NM_175500.3.
            
            Sequence Note: An in-frame AUG is located 73 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved, is in a weak Kozak sequence
            context, and expunges a signal peptide.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038547.1, SRR1660819.93033.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AK134807.1         2-38
            38-2871             AK038547.1         3-2836
            2872-2885           CJ237840.1         477-490
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 59.43 cM"
     gene            1..2885
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /note="glypican 5"
                     /db_xref="GeneID:103978"
                     /db_xref="MGI:MGI:1194894"
     exon            1..574
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             412..2130
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /codon_start=1
                     /product="glypican-5 precursor"
                     /protein_id="NP_780709.1"
                     /db_xref="CCDS:CCDS27329.1"
                     /db_xref="GeneID:103978"
                     /db_xref="MGI:MGI:1194894"
                     /translation="MDARTWRLGWRCLLLLALLGSTRSEGVESCEEVRKLFQWRLGGA
                     VKGLPEAPRAGPDLQVCLSKNPTCCTRKMEERYQIAARQDLQQVLQTSSSTLKLLISR
                     NAAAFQETLETLIRQAENYTSILFCNTYRNMALEAAASIQEFFTDVGLYLFGADVNPE
                     EFVNRFFDSLFPLVYNHLINPGVTDSSLQYSECIRMARQDVSPFGNIPKRVMGQMGRS
                     LLPGRTFLQALNLGIEVINTTDHIHFSKECSRALLKMQYCPHCQSLMLSKPCMGYCLN
                     VIRGCLAHMTELNPHWHAYIRSLEELSDAMHGTYDVEHVLLNFHLLVNDAVLQAHLNG
                     QKLLDQVNTICGHPVRTPTQSPRCTFDPSKEKHGMKISARNGEETLANRRKEFINSLR
                     LHGSFYGGLADQLCVNELAAPEGRPCWNGEEIVKSYAQRVVGNGIKAQSANPEVRVRG
                     TDPVVNQIIDKLKHVIQLLRGRSPKPNKWELLQPGSGGGMLENSSGDCDDEDGCGGSG
                     SGEVKRTLKITNWMPDSMNFSDVKQVHRADHGSTLDTTSTGCASGTESMALPLMGTLM
                     FLPWLW"
     exon            575..736
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            737..1431
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1565
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1691
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1812
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1972
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2885
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2848..2853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2876
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /note="major polyA site"
ORIGIN      
        1 gttcgcgggg ttggcgaacc gggctctgga gctccgcagg ctggcacagc ctaggttagt
       61 ggctgcgagc tgagccttag gcggctgcag cgataccggc cagtgccgga tgcttgccgt
      121 gtctcgcttc tccccggctc gtgttcggct tgcgcagcgc ccacccgccc tggtccggga
      181 gggacccggg cgatgctaag catgagaagt gcagcgtctc agcctcaggg cttcagggga
      241 gaaagcggag ctcttgcagg tgaagccgcg gtcccttcgc ggtcttcccg tggccgcctg
      301 cgcatcgaac ccttgccaga ttccgccacg catcttcctc ccgcgctgcc gatgctgggt
      361 gctctggaga gtgaggacca cagcggggag ggcgcgaccc gaacaccgag gatggatgcc
      421 cgcacctggc gtctgggctg gcgctgtctc cttctcctgg ctctccttgg atctacccga
      481 agcgagggcg tggagagctg cgaagaagtt cggaaacttt tccagtggcg gcttggggga
      541 gctgtcaaag ggctgccgga ggcgccgcgg gcaggacctg atcttcaggt ttgcctatcc
      601 aaaaacccaa cgtgctgtac caggaagatg gaggagaggt accagattgc agctcggcaa
      661 gatttgcagc aggtgcttca gacatccagc tcaacattaa agttgctaat atctcgaaat
      721 gcagctgctt ttcaagagac cctggaaacc ctcatcagac aagcagagaa ttacaccagt
      781 atccttttct gcaacaccta ccggaacatg gccttggagg ctgctgcttc cattcaggaa
      841 tttttcacgg atgttggact ctatttattt ggtgcggatg ttaatcccga ggaatttgta
      901 aacagattct ttgacagtct ttttcctctg gtctacaacc atcttataaa ccctggtgtg
      961 actgacagtt ctctgcaata ctcagaatgc attcgaatgg cccgccagga tgttagtcca
     1021 tttggcaaca ttcccaaaag agtcatgggg cagatgggga ggtcgctgct gcctggccgc
     1081 acattcttgc aggcgcttaa tctgggcatt gaggtcatca acaccacgga ccacatacac
     1141 ttctccaagg agtgcagtag ggccctcctg aagatgcagt attgtcctca ctgccagagc
     1201 ctgatgctca gtaagccctg tatggggtac tgcctcaatg tcatcagggg ctgcctggcc
     1261 cacatgactg agcttaatcc acactggcat gcttatatcc ggtctttgga agagttgtca
     1321 gatgcgatgc acggaaccta cgacgttgaa cacgtgctcc tgaacttcca cttgcttgtt
     1381 aatgatgccg tgttgcaggc tcacctcaat gggcagaaat tattggatca ggtgaatacg
     1441 atttgtggcc atccagtgag aacaccaaca cagagccccc gctgtacttt tgacccaagc
     1501 aaagagaaac atggaatgaa gatctctgca aggaatggtg aagagacact tgccaacaga
     1561 agaaaagaat tcatcaatag ccttaggctg catgggtcct tctatggtgg cctggctgac
     1621 cagctttgtg ttaatgaatt ggctgcccct gaaggaaggc cctgctggaa tggggaggaa
     1681 atagtcaaaa gctatgctca gcgtgtggtt gggaatggaa tcaaagccca gtctgcaaat
     1741 cctgaagtca gagtcagagg aaccgatcct gtggtaaatc agatcattga taaactgaag
     1801 catgtcatcc agttgttacg ggggagatca cccaagccca acaagtggga gctccttcag
     1861 ccaggcagtg gaggcggcat gcttgagaac tctagtggag actgtgatga cgaagatggc
     1921 tgtggaggat caggaagcgg agaagtaaag aggaccctga agatcacaaa ctggatgcca
     1981 gacagcatga acttcagtga tgtgaagcaa gtccatcgag cagaccatgg aagtactctg
     2041 gatacaacca gcaccggatg tgcgtcagga actgagtcta tggcactccc tctgatgggg
     2101 acactgatgt ttcttccctg gctttggtaa tgacatacct tactggagtc ttgatttatc
     2161 ccttatctcc ttatgtctgg aacagaagaa ctcttttaaa ataacgattg aaatgaggga
     2221 aaggtgtgtt attctaactt cttagatgcc aactggaatg ttcacacagt acctatcagt
     2281 caagattctg atagtctctt tcaaactaga tcttcttgac acctaactta aatcttctta
     2341 aattctaaca ttaaatctaa actgtgaaaa ctcagaagtg actattagta agaaggcttt
     2401 ggtgtatctg taattctata ttctgcttaa tcaaaccctt tccttttaac attgtgaatg
     2461 tcgacatttg agggtgtgtt tgtcaggcac agaacacaat aagtagtatt tcttaaagaa
     2521 ctgggaataa aaataacact aagtcaaagc acagaaaggc aagtattatg catgcatttt
     2581 aggggattct ttcatttttg caagttaaat aaaaagttac tacaagtctg tgagatatag
     2641 agtagattag cggggcgatt tcacataaat ttactgaagg cacttcataa tgttttacac
     2701 taaacctcct ctactgatct tagtaacgta gggatgtcca tgtgatgttc cttcacattt
     2761 gaaacgcact gtagtataat ggctctgctc ctcttttgtc tttccctaaa acaactcgct
     2821 atgtcagaaa gcacacttgt atactccaat aaagttgttg attatttgat gagaaacgtc
     2881 aaagc
//
