LOCUS       NP_780709                572 aa            linear   ROD 08-APR-2025
DEFINITION  glypican-5 precursor [Mus musculus].
ACCESSION   NP_780709
VERSION     NP_780709.1
DBSOURCE    REFSEQ: accession NM_175500.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 572)
  AUTHORS   Bosworth,A.P., Contreras,M., Sancho,L., Salas,I.H., Paumier,A.,
            Novak,S.W., Manor,U. and Allen,N.J.
  TITLE     Astrocyte glypican 5 regulates synapse maturation and stabilization
  JOURNAL   Cell Rep 44 (3), 115374 (2025)
   PUBMED   40048429
REFERENCE   2  (residues 1 to 572)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 572)
  AUTHORS   Wishart,T.F.L. and Lovicu,F.J.
  TITLE     Spatiotemporal Localisation of Heparan Sulphate Proteoglycans
            throughout Mouse Lens Morphogenesis
  JOURNAL   Cells 12 (10), 1364 (2023)
   PUBMED   37408198
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 572)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   5  (residues 1 to 572)
  AUTHORS   Wu,J., Li,H., Han,L., Sun,T., Tian,Y. and Wang,X.
  TITLE     The spatiotemporal expression pattern of Syndecans in murine
            embryonic teeth
  JOURNAL   Gene Expr Patterns 36, 119109 (2020)
   PUBMED   32220631
REFERENCE   6  (residues 1 to 572)
  AUTHORS   de Pontual,L., Yao,E., Callier,P., Faivre,L., Drouin,V., Cariou,S.,
            Van Haeringen,A., Genevieve,D., Goldenberg,A., Oufadem,M.,
            Manouvrier,S., Munnich,A., Vidigal,J.A., Vekemans,M., Lyonnet,S.,
            Henrion-Caude,A., Ventura,A. and Amiel,J.
  TITLE     Germline deletion of the miR-17 approximately 92 cluster causes
            skeletal and growth defects in humans
  JOURNAL   Nat Genet 43 (10), 1026-1030 (2011)
   PUBMED   21892160
  REMARK    Publication Status: Online-Only
REFERENCE   7  (residues 1 to 572)
  AUTHORS   Luxardi,G., Galli,A., Forlani,S., Lawson,K., Maina,F. and Dono,R.
  TITLE     Glypicans are differentially expressed during patterning and
            neurogenesis of early mouse brain
  JOURNAL   Biochem Biophys Res Commun 352 (1), 55-60 (2007)
   PUBMED   17107664
REFERENCE   8  (residues 1 to 572)
  AUTHORS   Ford-Perriss,M., Turner,K., Guimond,S., Apedaile,A., Haubeck,H.D.,
            Turnbull,J. and Murphy,M.
  TITLE     Localisation of specific heparan sulfate proteoglycans during the
            proliferative phase of brain development
  JOURNAL   Dev Dyn 227 (2), 170-184 (2003)
   PUBMED   12761845
REFERENCE   9  (residues 1 to 572)
  AUTHORS   Litwack,E.D., Ivins,J.K., Kumbasar,A., Paine-Saunders,S.,
            Stipp,C.S. and Lander,A.D.
  TITLE     Expression of the heparan sulfate proteoglycan glypican-1 in the
            developing rodent
  JOURNAL   Dev Dyn 211 (1), 72-87 (1998)
   PUBMED   9438425
REFERENCE   10 (residues 1 to 572)
  AUTHORS   Saunders,S., Paine-Saunders,S. and Lander,A.D.
  TITLE     Expression of the cell surface proteoglycan glypican-5 is
            developmentally regulated in kidney, limb, and brain
  JOURNAL   Dev Biol 190 (1), 78-93 (1997)
   PUBMED   9331333
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK134807.1, AK038547.1 and CJ237840.1.
            
            Sequence Note: An in-frame AUG is located 73 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved, is in a weak Kozak sequence
            context, and expunges a signal peptide.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038547.1, SRR1660819.93033.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 59.43 cM"
     Protein         1..572
                     /product="glypican-5 precursor"
                     /calculated_mol_wt=61021
     sig_peptide     1..24
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2789
     Region          20..569
                     /region_name="Glypican"
                     /note="pfam01153"
                     /db_xref="CDD:460084"
     Site            120
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            237
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            486
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            493
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            495
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            507
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            509
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     Site            527
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CAL5.1)"
     CDS             1..572
                     /gene="Gpc5"
                     /gene_synonym="A230034F01Rik"
                     /coded_by="NM_175500.4:412..2130"
                     /db_xref="CCDS:CCDS27329.1"
                     /db_xref="GeneID:103978"
                     /db_xref="MGI:MGI:1194894"
ORIGIN      
        1 mdartwrlgw rcllllallg strsegvesc eevrklfqwr lggavkglpe apragpdlqv
       61 clsknptcct rkmeeryqia arqdlqqvlq tssstlklli srnaaafqet letlirqaen
      121 ytsilfcnty rnmaleaaas iqefftdvgl ylfgadvnpe efvnrffdsl fplvynhlin
      181 pgvtdsslqy secirmarqd vspfgnipkr vmgqmgrsll pgrtflqaln lgievinttd
      241 hihfskecsr allkmqycph cqslmlskpc mgyclnvirg clahmtelnp hwhayirsle
      301 elsdamhgty dvehvllnfh llvndavlqa hlngqklldq vnticghpvr tptqsprctf
      361 dpskekhgmk isarngeetl anrrkefins lrlhgsfygg ladqlcvnel aapegrpcwn
      421 geeivksyaq rvvgngikaq sanpevrvrg tdpvvnqiid klkhviqllr grspkpnkwe
      481 llqpgsgggm lenssgdcdd edgcggsgsg evkrtlkitn wmpdsmnfsd vkqvhradhg
      541 stldttstgc asgtesmalp lmgtlmflpw lw
//
