LOCUS       NM_172715               2877 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus glycerol-3-phosphate acyltransferase 3 (Gpat3), mRNA.
ACCESSION   NM_172715 NM_001035278
VERSION     NM_172715.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Chen,H., Luo,S., Deng,X., Li,S., Mao,Y., Yan,J., Cheng,Y., Liu,X.,
            Pan,J. and Huang,H.
  TITLE     Pre-eclamptic foetal programming predisposes offspring to hepatic
            steatosis via DNA methylation
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167189 (2024)
   PUBMED   38648899
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Quiroga,I.Y., Pellon-Maison,M., Gonzalez,M.C., Coleman,R.A. and
            Gonzalez-Baro,M.R.
  TITLE     Triacylglycerol synthesis directed by glycerol-3-phosphate
            acyltransferases -3 and -4 is required for lipid droplet formation
            and the modulation of the inflammatory response during macrophage
            to foam cell transition
  JOURNAL   Atherosclerosis 316, 1-7 (2021)
   PUBMED   33260006
REFERENCE   3  (bases 1 to 2877)
  AUTHORS   Sim,M.F.M., Persiani,E., Talukder,M.M.U., Mcilroy,G.D., Roumane,A.,
            Edwardson,J.M. and Rochford,J.J.
  TITLE     Oligomers of the lipodystrophy protein seipin may co-ordinate GPAT3
            and AGPAT2 enzymes to facilitate adipocyte differentiation
  JOURNAL   Sci Rep 10 (1), 3259 (2020)
   PUBMED   32094408
  REMARK    GeneRIF: Oligomers of the lipodystrophy protein seipin may
            co-ordinate GPAT3 and AGPAT2 enzymes to facilitate adipocyte
            differentiation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2877)
  AUTHORS   Gao,M., Liu,L., Wang,X., Mak,H.Y., Liu,G. and Yang,H.
  TITLE     GPAT3 deficiency alleviates insulin resistance and hepatic
            steatosis in a mouse model of severe congenital generalized
            lipodystrophy
  JOURNAL   Hum Mol Genet 29 (3), 432-443 (2020)
   PUBMED   31873720
  REMARK    GeneRIF: GPAT3 deficiency alleviates insulin resistance and hepatic
            steatosis in a mouse model of severe congenital generalized
            lipodystrophy.
REFERENCE   5  (bases 1 to 2877)
  AUTHORS   Quiroga,I.Y., Pellon-Maison,M., Suchanek,A.L., Coleman,R.A. and
            Gonzalez-Baro,M.R.
  TITLE     Glycerol-3-phosphate acyltransferases 3 and 4 direct glycerolipid
            synthesis and affect functionality in activated macrophages
  JOURNAL   Biochem J 476 (1), 85-99 (2019)
   PUBMED   30523059
  REMARK    GeneRIF: glycerolipid synthesis is induced during macrophage
            activation. GPAT4 protein level and GPAT3/GPAT4 enzymatic activity
            increase during this process, and these two isoforms were required
            for the accumulation of cell TAG and PL.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2877)
  AUTHORS   Khatun,I., Clark,R.W., Vera,N.B., Kou,K., Erion,D.M., Coskran,T.,
            Bobrowski,W.F., Okerberg,C. and Goodwin,B.
  TITLE     Characterization of a Novel Intestinal Glycerol-3-phosphate
            Acyltransferase Pathway and Its Role in Lipid Homeostasis
  JOURNAL   J Biol Chem 291 (6), 2602-2615 (2016)
   PUBMED   26644473
  REMARK    GeneRIF: These studies identify GPAT3 as a novel enzyme involved in
            intestinal lipid metabolism.
REFERENCE   7  (bases 1 to 2877)
  AUTHORS   Cao,J., Perez,S., Goodwin,B., Lin,Q., Peng,H., Qadri,A., Zhou,Y.,
            Clark,R.W., Perreault,M., Tobin,J.F. and Gimeno,R.E.
  TITLE     Mice deleted for GPAT3 have reduced GPAT activity in white adipose
            tissue and altered energy and cholesterol homeostasis in
            diet-induced obesity
  JOURNAL   Am J Physiol Endocrinol Metab 306 (10), E1176-E1187 (2014)
   PUBMED   24714397
  REMARK    GeneRIF: GPAT3 is the primary GPAT in white adipose tissue and has
            an important role of the enzyme in regulating energy, glucose, and
            lipid homeostasis.
REFERENCE   8  (bases 1 to 2877)
  AUTHORS   Cao,J., Li,J.L., Li,D., Tobin,J.F. and Gimeno,R.E.
  TITLE     Molecular identification of microsomal
            acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de
            novo triacylglycerol synthesis
  JOURNAL   Proc Natl Acad Sci U S A 103 (52), 19695-19700 (2006)
   PUBMED   17170135
REFERENCE   9  (bases 1 to 2877)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   10 (bases 1 to 2877)
  AUTHORS   Starodub,O., Jolly,C.A., Atshaves,B.P., Roths,J.B., Murphy,E.J.,
            Kier,A.B. and Schroeder,F.
  TITLE     Sterol carrier protein-2 localization in endoplasmic reticulum and
            role in phospholipid formation
  JOURNAL   Am J Physiol Cell Physiol 279 (4), C1259-C1269 (2000)
   PUBMED   11003606
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY012233.1, AK160261.1, CN719213.1, AC107774.8 and AI462140.1.
            
            On or before Nov 3, 2006 this sequence version replaced
            NM_001035278.1, NM_172715.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096769.1, AK160261.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                BY012233.1         2-57
            57-939              AK160261.1         2-884
            940-1432            CN719213.1         151-643
            1433-1885           AK160261.1         1378-1830
            1886-2846           AC107774.8         57141-58101         c
            2847-2877           AI462140.1         1-31                c
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 48.52 cM"
     gene            1..2877
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /note="glycerol-3-phosphate acyltransferase 3"
                     /db_xref="GeneID:231510"
                     /db_xref="MGI:MGI:3603816"
     exon            1..133
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            134..427
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     CDS             287..1603
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /EC_number="2.3.1.51"
                     /EC_number="2.3.1.15"
                     /note="LPAAT-theta; 1-AGP acyltransferase 9;
                     lysophosphatidic acid acyltransferase theta;
                     acyl-CoA:glycerol-3-phosphate acyltransferase 3;
                     1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9;
                     1-acylglycerol-3-phosphate O-acyltransferase 9;
                     1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10"
                     /codon_start=1
                     /product="glycerol-3-phosphate acyltransferase 3"
                     /protein_id="NP_766303.1"
                     /db_xref="CCDS:CCDS19470.1"
                     /db_xref="GeneID:231510"
                     /db_xref="MGI:MGI:3603816"
                     /translation="MEGADLAVKLLSTWLTLVGGLILLPSAFGLSLGISEIYMKILVK
                     TLEWATLRIQKGAPKESALKNSASVGIIQRDESPMEKGLSGLRGRDFELSDVFYFSKK
                     GLEAIVEDEVTQRFSSEELVSWNLLTRTNVNFQYISPRLTMVWVLGVLVRYCFLLPLR
                     VTLAFIGISLLIIGTTLVGQLPDSSLKNWLSELVHLTCCRICVRSLSGTIHYHNKQYR
                     PQKGGICVANHTSPIDVLILATDGCYAMVGQVHGGLMGIIQRAMVKACPHVWFERSEI
                     KDRHLVTKRLKEHIADKKKLPILIFPEGTCINNTSVMMFKKGSFEIGGTIYPVAIKYN
                     PQFGDAFWNSSKYNLVSYLLRIMTSWAIVCDVWYMPPMTREEGEDAVQFANRVKSAIA
                     VQGGLTELPWDGGLKRAKVKDTFKEEQQKNYSKMIVGNGSPNLARD"
     exon            428..494
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            495..765
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            766..840
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            841..930
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1024
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1140
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1196
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1282
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1411
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1491
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..2877
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2854..2859
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /note="hexamer: AATAAA"
     polyA_site      2874
                     /gene="Gpat3"
                     /gene_synonym="1-AGPAT; 1-AGPAT 9; 4933407I02Rik;
                     4933408F15; A230097K15Rik; AGPAT 10; Agpat9; GPAT-3;
                     mGPAT3"
                     /note="major polyA site"
ORIGIN      
        1 gatgggggtg gggcaagggt agagtgtaga aaaagggagc ccggcgcccg aaagccactg
       61 gctacccgca tagcccgcag cccacgccga ggtgcaggga acctgcgggg atcctcgctg
      121 ccggagggcc tcgagctgaa gggaacgagg cgggcggcaa agactcctca gagcccggct
      181 ccgggctgga cgctgtgttt tccgaactgc ggctggactg cctgggactc tgacacctac
      241 cgctctcggc tttcctgggg acctcttcct gagggcgtgc tgagacatgg agggcgcaga
      301 cctggcggtg aagctcctgt ccacctggct gacgctggtg ggcggcctca tccttttacc
      361 ctcggccttc ggattatccc tgggtatctc ggagatctac atgaagatcc tggtgaaaac
      421 cttggagtgg gccacattac gaattcagaa aggagccccc aaggagtcag ctcttaaaaa
      481 ctcagcttct gtgggtatta tccaaagaga tgagtcaccc atggagaaag ggctctctgg
      541 tcttcgagga agggacttcg agctctctga tgtgttctat ttctccaaga aggggttgga
      601 agccattgtg gaggatgaag tgacccagag gttctcctcc gaagagctag tatcatggaa
      661 cctcctcaca cgaaccaatg tcaactttca gtacatcagt ccaaggctca ccatggtgtg
      721 ggtgctgggt gtcctagtgc gctattgctt cctgctacct ctgagggtca ccctggcttt
      781 cattgggatc agcttgctga ttataggaac tacactggtt ggccagcttc cagacagcag
      841 cctcaaaaac tggctgagtg agctggtgca tctgacctgc tgcaggatct gtgttcggtc
      901 cctatctggc accatccatt accataacaa gcagtacaga ccccagaagg gaggtatctg
      961 tgtcgccaat cacacctctc ccattgatgt cctaatcctg gccaccgatg gatgttacgc
     1021 catggttggg caggttcacg gtggattgat ggggatcatt cagagagcca tggttaaggc
     1081 ttgtcctcac gtctggtttg agcgctcaga aataaaggac agacatctag tgactaagag
     1141 attaaaagaa cacattgctg acaagaaaaa gttacccatt ctaatcttcc cagaaggtac
     1201 ttgcatcaac aatacttcag tcatgatgtt taaaaaggga agctttgaaa tcggaggaac
     1261 catctatcca gtggccataa agtataaccc ccagttcggc gatgccttct ggaacagtag
     1321 taaatacaac ttggtgagct acctgcttcg aatcatgacc agctgggcca ttgtctgcga
     1381 cgtgtggtac atgcctccca tgactagaga ggaaggagaa gacgcagttc agtttgcaaa
     1441 cagggtaaaa tccgctatcg ctgtacaagg agggctgacg gaacttccct gggatggagg
     1501 gctgaagaga gcaaaggtga aggacacctt caaggaagaa caacagaaga attacagcaa
     1561 gatgatcgtg ggcaacggat ctcccaacct ggcgagggac tgaagcatcg tggataaaca
     1621 tggcttcgca gaactggcct tcagaagtga aagatgatga tgttgttttg ttgtgttgtt
     1681 tttttgcttg tttgtttatt gttcattttt ttttctactt gattttgtct ctatcctcat
     1741 gccagaagct gaacctgcaa caggtgtcct ttttgatgtt ttcgttcttg tgttggctca
     1801 aggaattgta aggcccgttc ttgagtggaa acagactttt ataaaatgat gatgttgatg
     1861 gtgattgaat gaaatctttg tatggagaaa aagtgcttct agaatatgtt tggaatttgt
     1921 taataagata cttctcaaca atgactccaa actctgtaat ttgatttccc tttctttaat
     1981 ctttttttat ttttattttt attattattg ttatttttta aagacatggt tttatttatg
     2041 tagcccaggc tggcctcaaa ctcactttgt agccaagact ggctaagaac tgactattct
     2101 ggctccactt cccacgtgct aggattacag gcaggtgctt ccacacctag gtgtgacttc
     2161 agtaacatac caggtgaggt catcacacca tagtgcattt ccattatggt tacattctgc
     2221 tctcaggttg aaagaaaaat gggggattat ttttagtgaa agaacaaatt tgtggttatc
     2281 tgatctgaaa ggaatggaat ggagattatt tttttctcag gccttggagg accaggaccg
     2341 gttgctggct ggtcagattc atttaagaac caggatgagt ggtgttcatt cccacaacac
     2401 atctcattca gctgcagtgg agaaagcact gttagctttt cttgtttgta tgctaaagat
     2461 ttcttttgtc ttatgattgt tgttttgttt tgtttgtttg ttttgtaaca aaatgaaatt
     2521 gtccaaggga aggagccttt aaaacaaaag aaccacactt tctgcaggat acaagtgtga
     2581 gctatgcgga gcttgcctcc tgtccaccct gcccccaact tctctgtagt ggtttagccc
     2641 caaactcccc agccttgtca gcagaaaact aaatggtata actacctttt ggaaaactga
     2701 gccttaacat tttttaaagg ggaaacaaga gttctctgag tccaatagca tgagctatat
     2761 ttgatagtga tctatttaat actgctgtga gctcttgagg ctgttagctc cggagcaacc
     2821 ctgtgtagaa agtgaactgt cttactgact atgaataaat tttatatttt taaatga
//
