LOCUS       NM_001081089            2636 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus glycerol-3-phosphate acyltransferase 2, mitochondrial
            (Gpat2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001081089 XM_130488
VERSION     NM_001081089.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Dahlet,T., Truss,M., Frede,U., Al Adhami,H., Bardet,A.F., Dumas,M.,
            Vallet,J., Chicher,J., Hammann,P., Kottnik,S., Hansen,P., Luz,U.,
            Alvarez,G., Auclair,G., Hecht,J., Robinson,P.N., Hagemeier,C. and
            Weber,M.
  TITLE     E2F6 initiates stable epigenetic silencing of germline genes during
            embryonic development
  JOURNAL   Nat Commun 12 (1), 3582 (2021)
   PUBMED   34117224
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Shiromoto,Y., Kuramochi-Miyagawa,S., Nagamori,I., Chuma,S.,
            Arakawa,T., Nishimura,T., Hasuwa,H., Tachibana,T., Ikawa,M. and
            Nakano,T.
  TITLE     GPAT2 is required for piRNA biogenesis, transposon silencing, and
            maintenance of spermatogonia in micedagger
  JOURNAL   Biol Reprod 101 (1), 248-256 (2019)
   PUBMED   30951587
  REMARK    GeneRIF: GPAT2 is required for piRNA biogenesis, transposon
            silencing, and maintenance of spermatogonia in micedagger.
REFERENCE   3  (bases 1 to 2636)
  AUTHORS   Garcia-Fabiani,M.B., Montanaro,M.A., Stringa,P., Lacunza,E.,
            Cattaneo,E.R., Santana,M., Pellon-Maison,M. and Gonzalez-Baro,M.R.
  TITLE     Glycerol-3-phosphate acyltransferase 2 is essential for normal
            spermatogenesis
  JOURNAL   Biochem J 474 (18), 3093-3107 (2017)
   PUBMED   28729426
  REMARK    GeneRIF: Data suggest that Gpat2 is necessary for normal
            spermatogenesis; absence of Gpat2 up-regulates expression of
            apoptosis regulatory proteins, triggers apoptosis, and thereby
            decreases the number of dividing germ cells; Gpat2 knockdown (using
            RNA silencing) prevents germ cells from proceeding through
            pachytene stage.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2636)
  AUTHORS   Garcia-Fabiani,M.B., Montanaro,M.A., Lacunza,E., Cattaneo,E.R.,
            Coleman,R.A., Pellon-Maison,M. and Gonzalez-Baro,M.R.
  TITLE     Methylation of the Gpat2 promoter regulates transient expression
            during mouse spermatogenesis
  JOURNAL   Biochem J 471 (2), 211-220 (2015)
   PUBMED   26268560
  REMARK    GeneRIF: Thus, Gpat2 is expressed at a specific stage of
            spermatogenesis, consistent with piRNA synthesis and meiosis I
            prophase
REFERENCE   5  (bases 1 to 2636)
  AUTHORS   Shiromoto,Y., Kuramochi-Miyagawa,S., Daiba,A., Chuma,S.,
            Katanaya,A., Katsumata,A., Nishimura,K., Ohtaka,M., Nakanishi,M.,
            Nakamura,T., Yoshinaga,K., Asada,N., Nakamura,S., Yasunaga,T.,
            Kojima-Kita,K., Itou,D., Kimura,T. and Nakano,T.
  TITLE     GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis
            in germline stem cells
  JOURNAL   RNA 19 (6), 803-810 (2013)
   PUBMED   23611983
  REMARK    GeneRIF: GPAT2 is essential for the biogenesis of piRNA.
REFERENCE   6  (bases 1 to 2636)
  AUTHORS   Wang,S., Lee,D.P., Gong,N., Schwerbrock,N.M., Mashek,D.G.,
            Gonzalez-Baro,M.R., Stapleton,C., Li,L.O., Lewin,T.M. and
            Coleman,R.A.
  TITLE     Cloning and functional characterization of a novel mitochondrial
            N-ethylmaleimide-sensitive glycerol-3-phosphate acyltransferase
            (GPAT2)
  JOURNAL   Arch Biochem Biophys 465 (2), 347-358 (2007)
   PUBMED   17689486
REFERENCE   7  (bases 1 to 2636)
  AUTHORS   Gallardo,T.D., John,G.B., Shirley,L., Contreras,C.M., Akbay,E.A.,
            Haynie,J.M., Ward,S.E., Shidler,M.J. and Castrillon,D.H.
  TITLE     Genomewide discovery and classification of candidate ovarian
            fertility genes in the mouse
  JOURNAL   Genetics 177 (1), 179-194 (2007)
   PUBMED   17660561
  REMARK    GeneRIF: Transcripts highly enriched in ovary; gene identified as
            candidate female fertility factor.
REFERENCE   8  (bases 1 to 2636)
  AUTHORS   Harada,N., Hara,S., Yoshida,M., Zenitani,T., Mawatari,K.,
            Nakano,M., Takahashi,A., Hosaka,T., Yoshimoto,K. and Nakaya,Y.
  TITLE     Molecular cloning of a murine glycerol-3-phosphate
            acyltransferase-like protein 1 (xGPAT1)
  JOURNAL   Mol Cell Biochem 297 (1-2), 41-51 (2007)
   PUBMED   17013544
  REMARK    GeneRIF: A novel murine glycerol-3-phosphate acyltransferase-like
            protein 1 (named xGPAT1) has been cloned.
REFERENCE   9  (bases 1 to 2636)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
REFERENCE   10 (bases 1 to 2636)
  AUTHORS   Lewin,T.M., Schwerbrock,N.M., Lee,D.P. and Coleman,R.A.
  TITLE     Identification of a new glycerol-3-phosphate acyltransferase
            isoenzyme, mtGPAT2, in mitochondria
  JOURNAL   J Biol Chem 279 (14), 13488-13495 (2004)
   PUBMED   14724270
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB259571.1, AB257502.1 and AV466034.1.
            
            On Nov 6, 2012 this sequence version replaced NM_001081089.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC113165.1, AB257502.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849384, SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AB259571.1         19-100
            83-2566             AB257502.1         1-2484
            2567-2636           AV466034.1         262-331
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.0 cM"
     gene            1..2636
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /note="glycerol-3-phosphate acyltransferase 2,
                     mitochondrial"
                     /db_xref="GeneID:215456"
                     /db_xref="MGI:MGI:2684962"
     exon            1..23
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            24..154
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..2497
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /EC_number="2.3.1.51"
                     /EC_number="2.3.1.15"
                     /note="glycerol-3-phosphate acyltransferase-like 1;
                     GPAT-2; 1-acylglycerol-3-phosphate O-acyltransferase
                     GPAT2"
                     /codon_start=1
                     /product="glycerol-3-phosphate acyltransferase 2,
                     mitochondrial"
                     /protein_id="NP_001074558.2"
                     /db_xref="CCDS:CCDS38232.2"
                     /db_xref="GeneID:215456"
                     /db_xref="MGI:MGI:2684962"
                     /translation="MDTMLKSNPQTQQRSNHNGQETSLWSSSFGMKMEAITPFLGKYR
                     PFMGRCCQTCTPKSWESLFHRSIMDLGFCNVILVKEENTRFRGWLVRRLCYFLWSLEQ
                     HIPTSFDASQKIMENTGVQNLLSGRVPGAAGEGQAPELVKKEVQRILGHIQTTPRPFL
                     LRLFSWALLWFLNRLFLNVQLHKGQMKMVQKAVQEGSPLVFLSTHKSLLDGFLLPFVL
                     FSQGLGVVRVALDSRTCSPALRALLRKLGGLFLPPEVNLSLDNSEGILARAVVRATVE
                     ELLTSGQPLLIFLEEPPGSPGPRLSALGQAWLGVVIQAVQAGIISDATLVPVAIAYDL
                     VPDAPCNMNHDLAPLGLWTGALAVFRRLCNCWGCNRRVCVRVHLAQPFSLQEYTINAR
                     SCWDSRQTLEHLLQPIVLGECSVVPDTEKEQEWTPPTGLLLALKEEDQLLVRRLSRHV
                     LSASVASSAVMSTAIMATLLLLKHQKGVVLSQLLGEFSWLTEETLLRGFDVGFSGQLR
                     CLAQHTLSLLRAHVVLLRVHQGDLVVVPRPGPGLTHLARLSMELLPTFLSEAVGACAV
                     RGLLAGRVPPEGPWELQGIELLSQNELYRQILLLLHLLPQDLLLPQPCQSSYCYCQEV
                     LDRLIQCGLLVAEETPGSRPACDTGRQHLSAKLLWKPSGDFTDSESDDFEEPGGRCFR
                     LSQQSRCPDFFLFLCRLLSPILKAFAQAATFLHLGQLPDSEVAYSEKLFQFLQACAQE
                     EGIFECADPNLAISAVWTFKDLGVLQEMPSPTGPQLHLSPTFATRDNQDKLEQFIRQF
                     ICS"
     exon            155..268
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            269..342
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            343..447
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            448..576
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..676
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            677..810
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            811..910
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1123
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1246
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1329
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1448
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1519
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1778
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1927
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2005
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2137
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2138..2258
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2324
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2380
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..2628
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2603..2608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /note="hexamer: AATAGA"
     polyA_site      2628
                     /gene="Gpat2"
                     /gene_synonym="A530057A03Rik; Gm116; xGPAT1"
                     /note="major polyA site"
ORIGIN      
        1 cgggagttgg cggctggtgt gagttttttt tctaggtcag gtctgtgtgg agagagacac
       61 agtgcttgat tcactgatct tacagacctt catggatacc atgttgaaat ccaaccccca
      121 aacccagcag aggagtaacc acaatggtca agagaccagc ctttggtcct caagctttgg
      181 gatgaagatg gaggccatca ctccattcct gggaaagtat cgccccttta tgggccgctg
      241 ctgccagacc tgtactccta agagctggga gtccctcttc cacagaagca taatggacct
      301 gggcttctgc aacgtgatcc ttgtgaagga ggagaacacc aggttccggg gctggctggt
      361 tcgcaggctc tgctatttcc tgtggtccct ggagcagcac atacccacca gttttgatgc
      421 ctcccagaag atcatggaaa acactggggt gcagaacctc ctctcgggga gggttccggg
      481 agcggctgga gaaggccagg cgcctgaatt agtgaagaaa gaggtacagc gtatcctggg
      541 ccacattcag accacacccc gccccttctt gctcaggctg ttcagctggg cactgctgtg
      601 gttcctgaac cgcctcttcc tgaatgtaca acttcacaag ggacagatga agatggttca
      661 gaaggctgtg caggagggct ctccacttgt cttcctttct acacacaagt ccctcttgga
      721 tggattcctg ctgccttttg tactgttctc ccaaggcctc ggtgtggtcc gtgtggcttt
      781 agactcccgc acatgttccc ctgctctcag agctctattg aggaaacttg ggggactttt
      841 cctgccccca gaggtcaacc tctccttgga caactcagag ggcatccttg caagggctgt
      901 ggtccgtgca actgtggagg agctgcttac tagtggtcag cctctgctca tcttcctgga
      961 agagccccct gggtccccag ggcccaggct ttctgccctg ggccaggcct ggctaggagt
     1021 ggtaatccag gcggtccagg caggcattat ctcagatgcc acactggtac cagttgccat
     1081 tgcctatgac ctggttccag atgcaccatg taacatgaac catgatttgg ctcccctggg
     1141 gctatggaca ggagccttgg ctgtctttcg gagactgtgt aactgctggg gctgcaaccg
     1201 tagagtctgt gttcgggtgc accttgccca gccattttcc ctccaggaat acaccatcaa
     1261 tgctagaagt tgctgggata gcaggcagac cctggaacac ttgttgcagc ccattgtgct
     1321 aggtgaatgt agtgttgttc cggacactga gaaggagcag gagtggaccc caccaactgg
     1381 cctcctcctg gcccttaaag aggaggacca gctcctggtc agaagactga gccggcatgt
     1441 tctgagtgcc agtgtggcca gctccgcagt gatgagcaca gccatcatgg ccacactgct
     1501 tttgctgaag caccaaaagg gtgtggtcct gtcacagctc ctgggagaat tctcctggct
     1561 gacagaggag acactgctcc gaggttttga tgtgggcttc tcagggcagc tgcggtgcct
     1621 ggcccaacat acactgagcc tattgagggc acatgtggtt ctgctgcgtg tccaccaagg
     1681 ggacttggtg gtggtacctc ggcctggccc aggcctcaca cacctggcac gcttgagtat
     1741 ggaattgctg ccaaccttcc tgagtgaggc tgtgggtgcc tgtgcagtgc gggggttgct
     1801 ggcaggcaga gtaccacctg aggggccctg ggagctgcag ggcatagagc tactgagtca
     1861 gaatgaactg taccgccaga tcctactgct gctgcacctg ctacctcaag acctactgct
     1921 gccccagccc tgccagtctt cctactgcta ctgtcaggaa gtgctagacc ggctcatcca
     1981 gtgtggactc ctagttgctg aggagactcc aggctcccgg ccagcctgtg acacaggaag
     2041 acagcatctg agtgcaaagc tgctgtggaa gccgagtggg gactttactg acagtgagag
     2101 tgatgacttt gaggagccag gaggccgctg cttcaggctt agccagcaat cccgctgccc
     2161 tgacttcttc ctcttcctct gccgcttgct cagtcccata ctcaaggcct tcgcacaggc
     2221 ggccaccttc ctccacttag ggcagctgcc agattcagag gtggcctact cagagaagtt
     2281 gttccagttt ttacaggcct gtgcccaaga agaagggatc tttgagtgtg cagacccaaa
     2341 ccttgctatc agtgcagtct ggacgttcaa agatctgggg gtacttcagg agatgcctag
     2401 ccctacagga ccccagctcc acctgtcccc tacatttgcc acccgggaca atcaggacaa
     2461 gctggaacaa tttatccgac agttcatctg cagttagtac caggtggagc ctgatgggac
     2521 ttgtcagctt aaactcaagt gtgtgtctct gctcaggaca cagctaacct gaacctgtgt
     2581 ttgctcatca tttggtgtaa taaatagact aggttagttt cttcctaaaa aaaaaa
//
