LOCUS       NP_080265                531 aa            linear   ROD 17-DEC-2024
DEFINITION  pancreatic secretory granule membrane major glycoprotein GP2
            precursor [Mus musculus].
ACCESSION   NP_080265
VERSION     NP_080265.3
DBSOURCE    REFSEQ: accession NM_025989.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 531)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 531)
  AUTHORS   Martinez-Ramirez,A.S., Borders,T.L., Paul,L., Schipma,M., Wang,X.,
            Korobova,F., Wright,C.V. and Sosa-Pineda,B.
  TITLE     Specific Temporal Requirement of Prox1 Activity During Pancreatic
            Acinar Cell Development
  JOURNAL   Gastro Hep Adv 1 (5), 807-823 (2022)
   PUBMED   37829188
REFERENCE   3  (residues 1 to 531)
  AUTHORS   Prados,A., Onder,L., Cheng,H.W., Morbe,U., Lutge,M., Gil-Cruz,C.,
            Perez-Shibayama,C., Koliaraki,V., Ludewig,B. and Kollias,G.
  TITLE     Fibroblastic reticular cell lineage convergence in Peyer's patches
            governs intestinal immunity
  JOURNAL   Nat Immunol 22 (4), 510-519 (2021)
   PUBMED   33707780
REFERENCE   4  (residues 1 to 531)
  AUTHORS   Kurashima,Y., Kigoshi,T., Murasaki,S., Arai,F., Shimada,K.,
            Seki,N., Kim,Y.G., Hase,K., Ohno,H., Kawano,K., Ashida,H.,
            Suzuki,T., Morimoto,M., Saito,Y., Sasou,A., Goda,Y., Yuki,Y.,
            Inagaki,Y., Iijima,H., Suda,W., Hattori,M. and Kiyono,H.
  TITLE     Pancreatic glycoprotein 2 is a first line of defense for mucosal
            protection in intestinal inflammation
  JOURNAL   Nat Commun 12 (1), 1067 (2021)
   PUBMED   33594081
  REMARK    GeneRIF: Pancreatic glycoprotein 2 is a first line of defense for
            mucosal protection in intestinal inflammation.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 531)
  AUTHORS   He,M., Wu,B., Ye,W., Le,D.D., Sinclair,A.W., Padovano,V., Chen,Y.,
            Li,K.X., Sit,R., Tan,M., Caplan,M.J., Neff,N., Jan,Y.N.,
            Darmanis,S. and Jan,L.Y.
  TITLE     Chloride channels regulate differentiation and barrier functions of
            the mammalian airway
  JOURNAL   Elife 9, e53085 (2020)
   PUBMED   32286221
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 531)
  AUTHORS   de Lau,W., Kujala,P., Schneeberger,K., Middendorp,S., Li,V.S.,
            Barker,N., Martens,A., Hofhuis,F., DeKoter,R.P., Peters,P.J.,
            Nieuwenhuis,E. and Clevers,H.
  TITLE     Peyer's patch M cells derived from Lgr5(+) stem cells require SpiB
            and are induced by RankL in cultured 'miniguts'
  JOURNAL   Mol Cell Biol 32 (18), 3639-3647 (2012)
   PUBMED   22778137
REFERENCE   7  (residues 1 to 531)
  AUTHORS   Kanaya,T., Hase,K., Takahashi,D., Fukuda,S., Hoshino,K., Sasaki,I.,
            Hemmi,H., Knoop,K.A., Kumar,N., Sato,M., Katsuno,T., Yokosuka,O.,
            Toyooka,K., Nakai,K., Sakamoto,A., Kitahara,Y., Jinnohara,T.,
            McSorley,S.J., Kaisho,T., Williams,I.R. and Ohno,H.
  TITLE     The Ets transcription factor Spi-B is essential for the
            differentiation of intestinal microfold cells
  JOURNAL   Nat Immunol 13 (8), 729-736 (2012)
   PUBMED   22706340
  REMARK    Publication Status: Online-Only
REFERENCE   8  (residues 1 to 531)
  AUTHORS   Hase,K., Kawano,K., Nochi,T., Pontes,G.S., Fukuda,S., Ebisawa,M.,
            Kadokura,K., Tobe,T., Fujimura,Y., Kawano,S., Yabashi,A.,
            Waguri,S., Nakato,G., Kimura,S., Murakami,T., Iimura,M., Hamura,K.,
            Fukuoka,S., Lowe,A.W., Itoh,K., Kiyono,H. and Ohno,H.
  TITLE     Uptake through glycoprotein 2 of FimH(+) bacteria by M cells
            initiates mucosal immune response
  JOURNAL   Nature 462 (7270), 226-230 (2009)
   PUBMED   19907495
  REMARK    GeneRIF: glycoprotein 2 (GP2), specifically expressed on the apical
            plasma membrane of M cells among enterocytes, serves as a
            transcytotic receptor for mucosal antigens
REFERENCE   9  (residues 1 to 531)
  AUTHORS   Yu,S., Michie,S.A. and Lowe,A.W.
  TITLE     Absence of the major zymogen granule membrane protein, GP2, does
            not affect pancreatic morphology or secretion
  JOURNAL   J Biol Chem 279 (48), 50274-50279 (2004)
   PUBMED   15385539
  REMARK    GeneRIF: unlikely that GP2 serves a major intracellular role within
            the acinar cell, may be active after it is secreted
REFERENCE   10 (residues 1 to 531)
  AUTHORS   Kobayashi,K., Yanagihara,K., Ishiguro,K. and Fukuoka,S.
  TITLE     GP2/THP gene family of self-binding, GPI-anchored proteins forms a
            cluster at chromosome 7F1 region in mouse genome
  JOURNAL   Biochem Biophys Res Commun 322 (2), 659-664 (2004)
   PUBMED   15325280
  REMARK    GeneRIF: Tightly linked to homologue THP.  A Gp2 knockout mouse was
            established which does express THP.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125147.4.
            
            On Feb 9, 2019 this sequence version replaced NP_080265.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK009661.1, BC119573.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 63.88 cM"
     Protein         1..531
                     /product="pancreatic secretory granule membrane major
                     glycoprotein GP2 precursor"
                     /note="pancreatic secretory granule membrane major
                     glycoprotein GP2; pancreatic zymogen granule membrane
                     protein GP-2"
                     /calculated_mol_wt=57009
     sig_peptide     1..21
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2163
     Site            33
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          34..53
                     /region_name="Beta hairpin.
                     /evidence=ECO:0000250|UniProtKB:P55259"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          54..74
                     /region_name="D10C.
                     /evidence=ECO:0000250|UniProtKB:P55259"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Site            58
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Site            72
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Site            200
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          222..315
                     /region_name="ZP-N.
                     /evidence=ECO:0000250|UniProtKB:P07911"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          223..474
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     Site            256
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Site            285
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          316..339
                     /region_name="Flexible ZP-N/ZP-C linker.
                     /evidence=ECO:0000250|UniProtKB:P07911"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          340..478
                     /region_name="ZP-C.
                     /evidence=ECO:0000250|UniProtKB:P07911"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          340..351
                     /region_name="Internal hydrophobic patch (IHP).
                     /evidence=ECO:0000250|UniProtKB:P07911"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     Region          485..493
                     /region_name="External hydrophobic patch (EHP).
                     /evidence=ECO:0000250|UniProtKB:P07911"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9D733.3)"
     CDS             1..531
                     /gene="Gp2"
                     /gene_synonym="2310037I18Rik"
                     /coded_by="NM_025989.4:146..1741"
                     /db_xref="CCDS:CCDS21779.1"
                     /db_xref="GeneID:67133"
                     /db_xref="MGI:MGI:1914383"
ORIGIN      
        1 mvgcdllwla aascvltlvs pstihqgygr prnssnldld cgspdspssg icfdpcqnht
       61 vlndptrste nndssvawcd dnlhgwyrfv gdggvkmpet cvsvfrchts apmwlsgshp
      121 ilgdgivsht acanwnencc fwrsevqvka cseelgeyhv yklqgtpecs lryctdpsta
      181 pknceitcrp eeecvfqnnn wscvcrqdlh vsdsqslqpl ldcgdneikv kldkcllggm
      241 gfkeeiiayl ndrncngtmq depnnwvsmt spvvanycgn ilekngthai yrntlslatd
      301 fiirdfrvnv nfqcaypldm svsletalqp ivssltvdvd gagefnvkma lfqdqsytnp
      361 yegaevllpv esilyvgvll nrgdtsrfkl lltncyatps edrhdpvkyf iiknrcpnqr
      421 dstinvreng vssesrfsvq mfmfagnydl vflhcevylc dstteqcqps cstnrlrssr
      481 paidynrvld lgpitkrsaq ssatskgtph ttgfllawpm fflpvflall f
//
