LOCUS       NM_029364               3864 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus glucosamine (N-acetyl)-6-sulfatase (Gns), transcript
            variant 1, mRNA.
ACCESSION   NM_029364
VERSION     NM_029364.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Wang,F., Moen,D.R., Sauni,C., Kan,S.H., Li,S., Le,S.Q.,
            Lomenick,B., Zhang,X., Ekins,S., Singamsetty,S., Wood,J.,
            Dickson,P.I. and Chou,T.F.
  TITLE     Enzyme Replacement Therapy for Mucopolysaccharidosis IIID using
            Recombinant Human alpha-N-Acetylglucosamine-6-Sulfatase in Neonatal
            Mice
  JOURNAL   Mol Pharm 18 (1), 214-227 (2021)
   PUBMED   33320673
REFERENCE   3  (bases 1 to 3864)
  AUTHORS   Cajigas,I., Chakraborty,A., Swyter,K.R., Luo,H., Bastidas,M.,
            Nigro,M., Morris,E.R., Chen,S., VanGompel,M.J.W., Leib,D.,
            Kohtz,S.J., Martina,M., Koh,S., Ay,F. and Kohtz,J.D.
  TITLE     The Evf2 Ultraconserved Enhancer lncRNA Functionally and Spatially
            Organizes Megabase Distant Genes in the Developing Forebrain
  JOURNAL   Mol Cell 71 (6), 956-972 (2018)
   PUBMED   30146317
REFERENCE   4  (bases 1 to 3864)
  AUTHORS   Danyukova,T., Ariunbat,K., Thelen,M., Brocke-Ahmadinejad,N.,
            Mole,S.E. and Storch,S.
  TITLE     Loss of CLN7 results in depletion of soluble lysosomal proteins and
            impaired mTOR reactivation
  JOURNAL   Hum Mol Genet 27 (10), 1711-1722 (2018)
   PUBMED   29514215
REFERENCE   5  (bases 1 to 3864)
  AUTHORS   Roca,C., Motas,S., Marco,S., Ribera,A., Sanchez,V., Sanchez,X.,
            Bertolin,J., Leon,X., Perez,J., Garcia,M., Villacampa,P.,
            Ruberte,J., Pujol,A., Haurigot,V. and Bosch,F.
  TITLE     Disease correction by AAV-mediated gene therapy in a new mouse
            model of mucopolysaccharidosis type IIID
  JOURNAL   Hum Mol Genet 26 (8), 1535-1551 (2017)
   PUBMED   28334745
  REMARK    GeneRIF: Mice deficient in GNS showed lysosomal storage pathology
            and a phenotype that closely resembled human MPSIIID. Moreover,
            treatment of the GNS-deficient animals with GNS-encoding
            adeno-associated viral (AAV) vectors of serotype 9 delivered to the
            cerebrospinal fluid completely corrected pathological storage,
            improved lysosomal functionality in the CNS and somatic tissues,
            resolved neuroinflammation
REFERENCE   6  (bases 1 to 3864)
  AUTHORS   Ratzka,A., Mundlos,S. and Vortkamp,A.
  TITLE     Expression patterns of sulfatase genes in the developing mouse
            embryo
  JOURNAL   Dev Dyn 239 (6), 1779-1788 (2010)
   PUBMED   20503373
REFERENCE   7  (bases 1 to 3864)
  AUTHORS   Zito,E., Fraldi,A., Pepe,S., Annunziata,I., Kobinger,G., Di
            Natale,P., Ballabio,A. and Cosma,M.P.
  TITLE     Sulphatase activities are regulated by the interaction of
            sulphatase-modifying factor 1 with SUMF2
  JOURNAL   EMBO Rep 6 (7), 655-660 (2005)
   PUBMED   15962010
  REMARK    Erratum:[EMBO Rep. 2016 Dec;17(12):1901. doi:
            10.15252/embr.201570010.. PMID: 27909074]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140264.2.
            
            On Jun 28, 2018 this sequence version replaced NM_029364.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049162.1, AK054046.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               AC140264.2         120163-120431       c
            270-329             AC140264.2         114318-114377       c
            330-536             AC140264.2         110957-111163       c
            537-602             AC140264.2         109248-109313       c
            603-701             AC140264.2         108787-108885       c
            702-869             AC140264.2         107332-107499       c
            870-952             AC140264.2         105321-105403       c
            953-1071            AC140264.2         104230-104348       c
            1072-1175           AC140264.2         102078-102181       c
            1176-1277           AC140264.2         97319-97420         c
            1278-1385           AC140264.2         94790-94897         c
            1386-1496           AC140264.2         94040-94150         c
            1497-1657           AC140264.2         92680-92840         c
            1658-3864           AC140264.2         88267-90473         c
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 69.62 cM"
     gene            1..3864
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /note="glucosamine (N-acetyl)-6-sulfatase"
                     /db_xref="GeneID:75612"
                     /db_xref="MGI:MGI:1922862"
     exon            1..269
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1736
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /EC_number="3.1.6.14"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; N-acetylglucosamine-6-sulfatase"
                     /codon_start=1
                     /product="N-acetylglucosamine-6-sulfatase isoform 1
                     precursor"
                     /protein_id="NP_083640.1"
                     /db_xref="CCDS:CCDS24210.1"
                     /db_xref="GeneID:75612"
                     /db_xref="MGI:MGI:1922862"
                     /translation="MRLPSAAGPRPGRPRRLPALLLLPLLGGCLGLVGAARRPNVLLL
                     LTDDQDAELGGMTPLKKTKALIGEKGMTFSSAYVPSALCCPSRASILTGKYPHNHHVV
                     NNTLEGNCSSKAWQKIQEPYTFPAILKSVCGYQTFFAGKYLNEYGAPDAGGLEHIPLG
                     WSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANLSLDFLDYKSNSEPFF
                     MMISTPAPHSPWTAAPQYQKAFQNVIAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIRF
                     LDDAFRRRWQTLLSVDDLVEKLVKRLDSTGELDNTYIFYTSDNGYHTGQFSLPIDKRQ
                     LYEFDIKVPLLVRGPGIKPNQTSKMLVSNIDLGPTILDLAGYDLNKTQMDGMSLLPIL
                     KGDRNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTL
                     SSLWNLQYCEFDDQEVFVEVYNITADPDQITNIAKSIDPELLGKMNYRLMMLQSCSGP
                     TCRTPGVFDPGYRFDLRLMFNSHGSVRTRRFSKHPL"
     exon            270..329
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            330..536
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            537..602
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            603..701
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            702..869
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            870..952
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1071
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1175
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1277
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1385
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1496
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1657
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..3864
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3835..3840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /note="hexamer: ATTAAA"
     polyA_site      3856
                     /gene="Gns"
                     /gene_synonym="2610016K11Rik; G6S"
                     /note="major polyA site"
ORIGIN      
        1 gcctcacgtg atctcgccta gggagagaaa gcctgacccc gtctcctgcg ctcaccggcg
       61 tctctccgca gctctcggtt cccccgcgga gcttggccgc catgcggctc ccttcggcag
      121 ccgggccccg gccgggccgt ccccgccgcc tgccggcgct gctgctgctg ccgctgctgg
      181 gaggctgcct ggggctcgtg ggggcggctc gcaggcccaa cgtgctgctg ctgctcaccg
      241 acgaccagga cgcggagctc ggcggcatga cgccactgaa gaaaaccaag gccctcatcg
      301 gagagaaggg gatgactttc tctagcgcct atgtgcccag tgctctttgc tgccccagca
      361 gagccagcat cctgactgga aagtaccccc acaaccacca cgtcgttaac aacactttgg
      421 aagggaactg cagtagcaag gcctggcaga agatccagga gccgtacacc ttcccggcca
      481 tcctcaagtc ggtgtgcggc tatcagacct tttttgctgg gaagtattta aacgagtatg
      541 gagccccaga cgcaggcgga ctggagcaca tccccctggg ctggagttac tggtatgccc
      601 tggaaaagaa ttctaaatac tataactaca ccctctccat caacgggaag gcacggaagc
      661 acggcgagaa ctacagcgtg gactacctga cagatgttct ggccaacctc tccctggact
      721 tcctggacta caagtccaac tctgagccat tcttcatgat gatctccact ccagcgcccc
      781 actcaccgtg gacggctgct ccgcagtacc agaaggcttt ccagaatgtc atcgcaccaa
      841 gaaacaagaa cttcaacatc cacgggacga acaagcactg gctaatcagg caagccaaga
      901 ctcccatgac gaactcgtca atacggttct tagatgacgc cttcaggagg aggtggcaaa
      961 ccctgctctc agttgacgac cttgtggaga aactggtgaa gagactggac tcgaccggag
     1021 agctggacaa cacgtatatc ttctatacct cagacaacgg ctaccacaca gggcagttct
     1081 ctttgccaat agacaaacgg cagctgtatg agtttgatat caaagttcca ctgttggttc
     1141 gagggcccgg catcaaacca aaccagacca gcaagatgct ggtctcaaac atcgacttgg
     1201 gtcccaccat cctggacctc gctggctatg acctgaacaa gacacagatg gatgggatgt
     1261 ccctgctgcc cattctgaaa ggagacagaa acttaacctg gaggtcagac gtgctggtgg
     1321 aatatcaagg agaaggccgg aatgtcaccg acccgacctg cccttctttg agtcctggag
     1381 tgtctcaatg tttcccggac tgtgtgtgtg aagacgcgta caacaatacc tacgcctgcg
     1441 tgcggacact gtcatcattg tggaacctcc agtactgcga gttcgacgac caggaggtat
     1501 ttgtcgaagt ctacaatatc actgcagatc cagaccagat caccaacatt gccaagagta
     1561 tagaccctga gcttttggga aagatgaact atcgtctgat gatgctgcag tcctgttctg
     1621 ggccgacctg tcgcactcca ggggtctttg accctgggta caggtttgac ctgcgtctca
     1681 tgttcaacag tcacggcagc gtcagaacga gaaggttctc gaagcatccg ctctagcagc
     1741 ctctgcgttg gatccttgca cagccttttc cggtgccgtg gttggagtgg gagcccacgc
     1801 ctcgggagct ctggccttgc tctttcaagt cttgtttgaa aaataatcca gtcagctgac
     1861 ttccagtact gtgaatccgc ccctgtcaga agtggctgac cctgtttctc ctcagatgac
     1921 aaccaccact cctgaggtct ttgtcctctc cttgtccctt catcttggct gggcacagta
     1981 tatgagtccc cacgtggtcg agtctggatc tgtaaaactc ttctgataat ggcaatactt
     2041 tagggcagag ataaaacctt ttggtatttt agctagcccc caaatcaaaa gcactgtata
     2101 gcatttcaca ccgagcccct caccaaaact cacacagttg cgagaagagc tgctgtctgt
     2161 ggacacgtag agaaaggaca ttcatgacat ccctgtggtg tggttaatgt gcgacgtgtc
     2221 gcccaagctc tgccctaaga agcttcttag tgactccagc cacctgagag cgcccaggag
     2281 caccaggggg ggccagatgc gcttcctgag ctcttggatc agtgcgctga agggccgcac
     2341 ttacaagtgc ctgtctggag ggttctcaag tgtcagcgcc acacaagtca tcctgcctgg
     2401 ctgcagttag ctgtcattgt cattgaaaat cttagtgtct cctggtggag gggaaggacc
     2461 ggctgcctca ccccactccc tgatctcaca aaagtgcgag tttccagtcc tgactaacta
     2521 gggctccagt cagtattccc ctggccggct tcccagatgc cctggacggc actctgttcc
     2581 ttaaagtcca gttccaccct gacgtccaca gaggcagcac ggagtggcga aactcggttc
     2641 cagcctgtcg tcggcagtga gcgggtttag ctttacacag cctctgccca gctttaactt
     2701 gtaagacagc tatgagtcac tttgagaatt ccagaggcca tgttgggggg acagccattg
     2761 agtttattcc tttacctcat gtgaccccag caactttggt gatactgaga ggccagcagg
     2821 cagtagagtg gacacctcgg ccgtggccac tttagagggg gaaaagttta agttttaaac
     2881 taaaagttac agaattgaat gtggataccg gcttcattca cccccacttg gattctaaac
     2941 atgaccagtg aaatagtgaa agctgaccac agttgttccc aaataaaggt cggcagctcc
     3001 taccaggtct gggattgaga tgttagagtc ttcagtttta tagccggctc ctgaaagcag
     3061 gcgacatcag agtggtaaca gtcagtaggc tgcagtaggt gtcctacctc gaggccctca
     3121 gatggaggta acacattagg attgtgggac acaagatcct ctctgaacct cctggttaag
     3181 gaacgttcct gcaaacattc aagtagacct tctataacag actgcagcgt gcatggcctc
     3241 tacccacaag agatgcactg caggaggccg gccctgttct ctgtactcag cattttgaca
     3301 tctcctttga aggccaggta tatgtgtacc agtgctcttc aaagggagaa ccattaaaac
     3361 caacatggaa tgcagggact tcgccttata agatttccag ttttaaaact gcccctcaga
     3421 tacaatttca aagtagggta cacattagag tgtctttgag ttcaacaagt tggtactgtt
     3481 gcaaacacct tctgaattgc cagcgcccgg gtttttatgt aaaaatagag tggctatgga
     3541 agttacacct tcatctgtgg gaagtattct caaacgtagg ggtatcttta tcttatgttt
     3601 tctaatggaa gatggcagcc cagaatcctc aagacaaagc aaggggtatc agctgatggc
     3661 cgcgggactg atgggtgtcc tgggttcttt agctcaccag cactttgcgc tcaatggact
     3721 ttgtaaattc tcatcagctg ttgtagaagt tcctctgcct ggagctgtca tctcagccgc
     3781 tcccttagaa agtgcccttc aacggaccct gttcactgct gcacttctca atgaattaaa
     3841 actgacttct gttctagtgg caat
//
