LOCUS       NM_025278               4259 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus guanine nucleotide binding protein (G protein), gamma
            12 (Gng12), transcript variant 2, mRNA.
ACCESSION   NM_025278
VERSION     NM_025278.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Yim,Y.Y., McDonald,W.H., Betke,K.M., Kaya,A., Hyde,K., Erreger,K.,
            Gilsbach,R., Hein,L. and Hamm,H.E.
  TITLE     Specificities of Gbetagamma subunits for the SNARE complex before
            and after stimulation of alpha2a-adrenergic receptors
  JOURNAL   Sci Signal 14 (714), eabc4970 (2021)
   PUBMED   34932372
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Byrnes,L.E., Wong,D.M., Subramaniam,M., Meyer,N.P., Gilchrist,C.L.,
            Knox,S.M., Tward,A.D., Ye,C.J. and Sneddon,J.B.
  TITLE     Lineage dynamics of murine pancreatic development at single-cell
            resolution
  JOURNAL   Nat Commun 9 (1), 3922 (2018)
   PUBMED   30254276
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4259)
  AUTHORS   Moon,A.M., Stauffer,A.M., Schwindinger,W.F., Sheridan,K.,
            Firment,A. and Robishaw,J.D.
  TITLE     Disruption of G-protein gamma5 subtype causes embryonic lethality
            in mice
  JOURNAL   PLoS One 9 (3), e90970 (2014)
   PUBMED   24599258
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4259)
  AUTHORS   Ahmed,S.M., Theriault,B.L., Uppalapati,M., Chiu,C.W., Gallie,B.L.,
            Sidhu,S.S. and Angers,S.
  TITLE     KIF14 negatively regulates Rap1a-Radil signaling during breast
            cancer progression
  JOURNAL   J Cell Biol 199 (6), 951-967 (2012)
   PUBMED   23209302
REFERENCE   5  (bases 1 to 4259)
  AUTHORS   Oeschger,F.M., Wang,W.Z., Lee,S., Garcia-Moreno,F., Goffinet,A.M.,
            Arbones,M.L., Rakic,S. and Molnar,Z.
  TITLE     Gene expression analysis of the embryonic subplate
  JOURNAL   Cereb Cortex 22 (6), 1343-1359 (2012)
   PUBMED   21862448
REFERENCE   6  (bases 1 to 4259)
  AUTHORS   Larson,K.C., Lipko,M., Dabrowski,M. and Draper,M.P.
  TITLE     Gng12 is a novel negative regulator of LPS-induced inflammation in
            the microglial cell line BV-2
  JOURNAL   Inflamm Res 59 (1), 15-22 (2010)
   PUBMED   19568691
  REMARK    GeneRIF: Modulating Gng12 mRNA levels using RNAi revealed a novel
            role for the factor in the negative regulation of the overall
            inflammatory response as based on effects on nitrite and TNFalpha
            levels.
            Erratum:[Inflamm Res. 2009 Oct;58(10):725]
REFERENCE   7  (bases 1 to 4259)
  AUTHORS   Downes,G.B., Gilbert,D.J., Copeland,N.G., Gautam,N. and
            Jenkins,N.A.
  TITLE     Chromosomal mapping of five mouse G protein gamma subunits
  JOURNAL   Genomics 57 (1), 173-176 (1999)
   PUBMED   10191100
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB851888.1, AK159872.1 and AC107650.19.
            
            On Apr 16, 2010 this sequence version replaced NM_025278.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3, 4, 5 and 6 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159872.1, SRR1660815.164812.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                BB851888.1         2-64
            64-1039             AK159872.1         6-981
            1040-4259           AC107650.19        14947-18166         c
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 30.68 cM"
     gene            1..4259
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="guanine nucleotide binding protein (G protein),
                     gamma 12"
                     /db_xref="GeneID:14701"
                     /db_xref="MGI:MGI:1336171"
     exon            1..131
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /inference="alignment:Splign:2.1.0"
     exon            132..189
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /inference="alignment:Splign:2.1.0"
     exon            190..242
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /inference="alignment:Splign:2.1.0"
     exon            243..361
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..487
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="heterotrimeric guanine nucleotide-binding protein
                     3a"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein
                     G(I)/G(S)/G(O) subunit gamma-12"
                     /protein_id="NP_079554.1"
                     /db_xref="CCDS:CCDS39502.1"
                     /db_xref="GeneID:14701"
                     /db_xref="MGI:MGI:1336171"
                     /translation="MSSKTASTNSIAQARRTVQQLRLEASIERIKVSKASADLMSYCE
                     EHARSDPLLMGIPTSENPFKDKKTCIIL"
     exon            362..4259
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4117..4122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="hexamer: AATAAA"
     polyA_site      4144
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
     regulatory      4172..4177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="hexamer: ATTAAA"
     polyA_site      4193
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="major polyA site"
     regulatory      4229..4234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
                     /note="hexamer: AATAAA"
     polyA_site      4248
                     /gene="Gng12"
                     /gene_synonym="2010305F15Rik; Hg3a"
ORIGIN      
        1 acgcgcgagg ggaggagctg gagcggagta gggaggagag gtcgggcggt cccgggagcg
       61 cggagagccg gactcccagg cggcggcagc agcggcggcg gcggcgacga cggcgaagag
      121 tgagtgccaa ggttcatatg ggaaggactt tggggtgagc atcttctcta tttccagctg
      181 gcttttctga ttcaccccac catttaaaac ctggaggcac tgggccacac aaagccttgc
      241 tgattttcag aaagaagact catcaaagat gtccagcaag acggcaagca ccaacagcat
      301 agcccaagcc aggagaactg tgcagcagct gagattggaa gcctccatcg aaagaataaa
      361 ggtctcaaaa gcatcagcag acctgatgtc atactgtgag gagcatgccc ggagcgaccc
      421 cctgctgatg ggcataccga cctcagaaaa cccgttcaag gataagaaga cctgcatcat
      481 cttatagtgg accaggaagc gccccttgcc tcttaacgca aaccacagca gcaacctgaa
      541 gggattcctt cagcttacct ggtaaccaca gctagtaact aaaacaccct tctctcggaa
      601 taatagaccc tgaagtctct ctttttcaag ttgtcctttc ttcacccttt actgatttaa
      661 tacagaataa caatcttatt ttctatttga taactatggt atcatattgg gttactgtat
      721 aaggaaaatg gcaggggagt tgtgggaagc ttgtctttac aaaatataat tgattaagat
      781 atgtcaagac ctacattgtc taagcaccgg caaattaaaa tgtcgagaat cacttcagtc
      841 aaaaaccttt atattctgtt cttaataatg tttgtgccaa cctatatccc atgtaaggga
      901 tctggggagg aggcatgtgt ctacaaccat acctttttgc actatgggca ctaaccaccc
      961 tgaaacttcc tgcggtagct ccctcccttc agagttacat cattatcctg actctgtgta
     1021 ggtaaatttc cgtgaaattt ttgtacaaaa aaaaaggtaa tgaaagaacg ttgcaaagat
     1081 catctgcatt ataatgagtt gatgctgttc tcactcctct cttggaattg tgctggcccc
     1141 ttagtctaca ataaactgtg ccaattaaaa acctaaggct aaaactgaaa gccctttgat
     1201 ggggtcttaa ctcatatcag tcatttgggc ttctctgatc ctgaggctaa gaaaggggaa
     1261 gagaccctca ggaggcagct tccactccag ggctcttgat ctctgctgga ttgggggtgg
     1321 ccacctcaga aacttccacc ctcatgactg gaatggaaga ggggaccgag agcctcacaa
     1381 tctcggagag ggaggagaaa ttcttaaaaa cagctgctct cctgcgccca gcttcacagg
     1441 cagccctgcc cctttctcct caccagcatg gtacctgccc ttactgctag agcagctgct
     1501 tgtagaggga cattccctcc ttcccagttt taactggtgg accacagtgg ggggaaaaac
     1561 attcaagcga tataaagaca cttgggctct ttgcagatgc ctatacttcc aacactacca
     1621 tgtccacaaa ccaccctggg ggagggccct tccaaaggga ggcttgctag tttcagcgtc
     1681 tagcagttgg gtcctcactt ttactccaat tgtgaaaata gcccacgtac cctcgcagtg
     1741 tccagtaggg atcccagagg cacataacca agaaaggatt ttgactttgt cacagtgact
     1801 atttaaaata atctattcga agtccaaacc aaacacaaag cctgtgatat tttaggttat
     1861 taaggtaact gctaatgaag gattttaaaa agtgtcctca aaaaggactt agccccggga
     1921 gttgtttata aaatttcccc cacttgtata cagtgtgcac taaaagaaaa tgtattttaa
     1981 tatctaatgc ctgggctctg agcgtcatgc ttcttggtgg taaacatgca gtcctgttcc
     2041 taagtgactc agaggcatca gaatttctcc acgttaccca tctgcttggc actcggaact
     2101 gagcgtgtga aatccatagc actgcccaca acctgttctc actgcttagc tcccagctgg
     2161 attaaagaca cctgctcagg cgggagagag agagagagag cgagctttta ccttggaaaa
     2221 ggtaaagatg gaaatgtaca ccaaaaaaga caatttttac atttaatgga acattctttt
     2281 tttttacaag tatatttttc tactgatagt ttcagaacac taatcttata ttcactctaa
     2341 tcttaaacat gtttctttaa atatttataa ggcagtttat tacagaatat tttcatgcaa
     2401 tcatgtgcac attattggta gcaaacatag tatatccttt agtactttaa catatttttg
     2461 ttaaaatact tttaatggta agaaatgaac ttgaggtccc aggaggtttt gttgcctttt
     2521 cattgattag agacaataaa tatcttgtaa cttcctaacc agatctgagc tgtgctcaca
     2581 ataataataa tgaaatcaga ttctttgatg ctggactcag gagggaaatc attagccaac
     2641 tgttgactta cttatagcta gatgtctatg tgagaaagta taatatatat atatacacat
     2701 atatatgaca tgtaagagtc acttttattt atctgtcttt gttcacttat gaagccggta
     2761 actgcagcag tatgttggtg atgtcatgat gcacagaagt cccatgtgga gtgtttttcc
     2821 cacactgaca acttggcttc ctttctgtgt gttcagtctg ttgtctgaac taacactccc
     2881 gcgagcacta tactctttat actctgatcc ccctagttca tcttaaattt gtccgtggcc
     2941 ctggcaagat agcgtacaca agattccatg actccagagc atcttgaaga aacatacata
     3001 ttttgaaaga ggggaaatgt agcagatagt tcacaagctg cgggttgtag ctaaatattc
     3061 catttctttg aaatcatgtt tctaaattct ttaccatcag aaagaaaagg agtgtcatac
     3121 actttcaagg gaaggcttgg tctgcgtttt ctgtgtttgg attattttta tactttgctg
     3181 atctttaagc tatccatggg ggaaatttta taccaacgag ttaataattc tcattcatcg
     3241 tttacacaat gtaacatgtg tcatactggg gccagcgaga tggctcagta ggtaaaggtg
     3301 cttgatgcta agcccggcag cctgtgtttc atctacagga tgcacaacat aaaagaaaag
     3361 atctgattcc cgcaggttct cttctgacct acacacacac acactaaaat aacatttaaa
     3421 aatatgtgcc aaattatatt tgttcgggtg ccaccttcca ccagcttacc actacggtag
     3481 aactgtcaaa ttcatctccc tgaatttgtc ttaaaggggt gtccatgcac aggcccaaga
     3541 gtcacctcca atgaaataaa tgtaatactg aagtatgcca tgatgtttgt tgttttcttt
     3601 catcgtaagc ctgtaagcag gaaaaatacg tcaaatcaga tagaatagag catttaccag
     3661 tggtcgatgg cctggtcagt cctgtgccgg gtgacttagg accaggcacg tcagctctct
     3721 gagcctcccc ttcccttgtt gtcacaaggg aatagaagca gaagaagctg agagcctccc
     3781 tattcccaga tgccctggtg gaatgacctg cctctctgcc gtttctgcca acgtgttggt
     3841 gctataagct gcttcaaata ccagtttgtc tgtagtgtgt actcacctaa tcacttgtta
     3901 tccagtgcct gttctaggtt tatggactta actatttctg tgatgtttca tttttagcca
     3961 tgttaactcc taacacatat tctcttatgt ctcagtaaag tttcatttga taagttgttg
     4021 agattctgtt atttgataat attcttcggc tgtccatcca gcatcttaat cactttaaaa
     4081 ctgtgattgt tatttgcaac tctgttcttt ggaaagaata aaagcatttt ttttcacttg
     4141 ctaacatgct cacaaatgtg agagaagagt cattaaaagc tttacttact gggtcagtgc
     4201 gtcattgact cctttctgtg ttttgcccaa taaattaata aaagactatt tcttccctt
//
