LOCUS       NM_001190414            5610 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus glucosamine
            (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne),
            transcript variant 1, mRNA.
ACCESSION   NM_001190414 XR_106256 XR_107586
VERSION     NM_001190414.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Neu,C.T., Weilepp,L., Bork,K., Gesper,A. and Horstkorte,R.
  TITLE     GNE deficiency impairs Myogenesis in C2C12 cells and cannot be
            rescued by ManNAc supplementation
  JOURNAL   Glycobiology 34 (3) (2024)
   PUBMED   38224318
  REMARK    GeneRIF: GNE deficiency impairs Myogenesis in C2C12 cells and
            cannot be rescued by ManNAc supplementation.
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5610)
  AUTHORS   Huang,L., Kondo,Y., Cao,L., Han,J., Li,T., Zuo,B., Yang,F., Li,Y.,
            Ma,Z., Bai,X., Jiang,M., Ruan,C. and Xia,L.
  TITLE     Novel GNE missense variants impair de novo sialylation and cause
            defective angiogenesis in the developing brain in mice
  JOURNAL   Blood Adv 8 (4), 991-1001 (2024)
   PUBMED   38237079
  REMARK    GeneRIF: Novel GNE missense variants impair de novo sialylation and
            cause defective angiogenesis in the developing brain in mice.
REFERENCE   4  (bases 1 to 5610)
  AUTHORS   Harazi,A., Yakovlev,L., Ilouz,N., Selke,P., Horstkorte,R.,
            Fellig,Y., Lahat,O., Lifschytz,T., Abudi,N., Abramovitch,R.,
            Argov,Z. and Mitrani-Rosenbaum,S.
  TITLE     Induced Muscle and Liver Absence of Gne in Postnatal Mice Does Not
            Result in Structural or Functional Muscle Impairment
  JOURNAL   J Neuromuscul Dis 11 (5), 905-917 (2024)
   PUBMED   38875046
  REMARK    GeneRIF: Induced Muscle and Liver Absence of Gne in Postnatal Mice
            Does Not Result in Structural or Functional Muscle Impairment.
REFERENCE   5  (bases 1 to 5610)
  AUTHORS   Peng,J., Yu,L., Huang,L., Paschoal,V.A., Chu,H., de Souza,C.O.,
            Varre,J.V., Oh,D.Y., Kohler,J.J., Xiao,X., Xu,L., Holland,W.L.,
            Shaul,P.W. and Mineo,C.
  TITLE     Hepatic sialic acid synthesis modulates glucose homeostasis in both
            liver and skeletal muscle
  JOURNAL   Mol Metab 78, 101812 (2023)
   PUBMED   37777009
REFERENCE   6  (bases 1 to 5610)
  AUTHORS   Reinke,S.O. and Hinderlich,S.
  TITLE     Prediction of three different isoforms of the human
            UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
  JOURNAL   FEBS Lett 581 (17), 3327-3331 (2007)
   PUBMED   17597614
REFERENCE   7  (bases 1 to 5610)
  AUTHORS   Ohnemus,S., Kanzler,B., Jerome-Majewska,L.A., Papaioannou,V.E.,
            Boehm,T. and Mallo,M.
  TITLE     Aortic arch and pharyngeal phenotype in the absence of
            BMP-dependent neural crest in the mouse
  JOURNAL   Mech Dev 119 (2), 127-135 (2002)
   PUBMED   12464426
REFERENCE   8  (bases 1 to 5610)
  AUTHORS   Schwarzkopf,M., Knobeloch,K.P., Rohde,E., Hinderlich,S.,
            Wiechens,N., Lucka,L., Horak,I., Reutter,W. and Horstkorte,R.
  TITLE     Sialylation is essential for early development in mice
  JOURNAL   Proc Natl Acad Sci U S A 99 (8), 5267-5270 (2002)
   PUBMED   11929971
  REMARK    GeneRIF: inactivation of the UDP-GlcNAc 2-epimerase by gene
            targeting causes early embryonic lethality in mice, thereby
            emphasizing the fundamental role of this bifunctional enzyme and
            sialylation during development
REFERENCE   9  (bases 1 to 5610)
  AUTHORS   Horstkorte,R., Nohring,S., Danker,K., Effertz,K., Reutter,W. and
            Lucka,L.
  TITLE     Protein kinase C phosphorylates and regulates
            UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
  JOURNAL   FEBS Lett 470 (3), 315-318 (2000)
   PUBMED   10745088
REFERENCE   10 (bases 1 to 5610)
  AUTHORS   Horstkorte,R., Nohring,S., Wiechens,N., Schwarzkopf,M., Danker,K.,
            Reutter,W. and Lucka,L.
  TITLE     Tissue expression and amino acid sequence of murine
            UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
  JOURNAL   Eur J Biochem 260 (3), 923-927 (1999)
   PUBMED   10103025
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB856323.1, BY036706.1, BY310771.1, AK136573.1, AK033507.1 and
            AL732563.5.
            
            On Nov 4, 2017 this sequence version replaced NM_001190414.1.
            
            Transcript Variant: This variant (1) contains a different 5'
            terminal exon compared to transcript variant 1, which results in
            translation initiation from an in-frame downstream start codon. The
            resulting protein (isoform 1) has a shorter N-terminus compared to
            isoform 2. This variant has been called GNE1 in the scientific
            literature.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK170707.1, SRR9219382.224566.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BB856323.1         62-80
            20-20               BY036706.1         62-62
            21-44               BB856323.1         82-105
            45-397              BY310771.1         2-354
            398-2854            AK136573.1         173-2629
            2855-5404           AK033507.1         746-3295
            5405-5610           AL732563.5         66931-67136         c
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 23.1 cM"
     gene            1..5610
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="glucosamine
                     (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase"
                     /db_xref="GeneID:50798"
                     /db_xref="MGI:MGI:1354951"
     exon            1..356
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            357..562
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     CDS             399..2567
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /EC_number="2.7.1.60"
                     /EC_number="3.2.1.183"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bifunctional UDP-N-acetylglucosamine
                     2-epimerase/N-acetylmannosamine kinase;
                     UDP-GlcNAc-2-epimerase/ManAc kinase"
                     /codon_start=1
                     /product="bifunctional UDP-N-acetylglucosamine
                     2-epimerase/N-acetylmannosamine kinase isoform 1"
                     /protein_id="NP_001177343.1"
                     /db_xref="CCDS:CCDS51170.1"
                     /db_xref="GeneID:50798"
                     /db_xref="MGI:MGI:1354951"
                     /translation="MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVV
                     VLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRL
                     KPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCC
                     TRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVAL
                     QHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNF
                     RAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHV
                     RDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPV
                     KENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLIL
                     QMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTL
                     HLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAE
                     LGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGA
                     LHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDV
                     IRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH"
     exon            563..1014
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1167
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1380
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1468
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1679
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1809
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..2031
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2214
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2331
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..5610
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2833..2838
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="hexamer: AATAAA"
     polyA_site      2859
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="major polyA site"
     regulatory      5376..5381
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="hexamer: AATAAA"
     polyA_site      5406
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
     regulatory      5475..5480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="hexamer: CATAAA"
     polyA_site      5499
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
     regulatory      5588..5593
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
                     /note="hexamer: TATAAA"
     polyA_site      5610
                     /gene="Gne"
                     /gene_synonym="2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1"
ORIGIN      
        1 gcgaatgcag gagcaatggc tggggctggg tcgagcgccg agtcgtcgag cggcgccggg
       61 tagcggaagc caccgggtgc gcttgccggg gcctgccctg tggacccgct ggacgcgagc
      121 ctgccaagtg ccgaggtggc gccggaggga actaacccgg cggaggccac gagtcagtcg
      181 gtgactcacg agcatcgtgc caggctccgg cacctctgct gcagctgtgg gcctcgcgct
      241 gggcgtccgg gttctacgca gcagggcgcc tctcccgccc ggtgcgttgc tccggcgtgg
      301 gatcccggag ctgtcagact cagcgtgcgg gtgaatgggg accggggctt gcagaggaac
      361 tctattttaa gaaactctca agtaaaaaga agcaagtcat ggagaagaac gggaacaacc
      421 gaaagctccg ggtttgcgtt gccacctgca accgagctga ctactccaaa ctggccccga
      481 tcatgttcgg catcaagaca gagcccgcgt tctttgagtt ggacgtggtg gtgctcggct
      541 cccacctgat tgacgactat ggaaacacat accgcatgat tgagcaagat gactttgaca
      601 ttaacaccag gctccacacg attgttagag gggaagatga agcggccatg gtagagtcgg
      661 taggcctagc gctcgtgaag ctaccggacg tcctcaatcg cctgaagccc gacatcatga
      721 ttgttcacgg agaccgattt gacgcccttg ctctggctac gtctgctgcc ttgatgaaca
      781 tccgcatcct tcacattgaa ggaggcgagg tcagcgggac cattgatgac tctatcagac
      841 acgccataac aaaactggct cactaccatg tgtgctgcac tagaagtgca gagcagcacc
      901 tgatctctat gtgcgaggac cacgaccgca tcctgttggc aggctgccct tcctatgaca
      961 aactgctctc cgccaagaac aaagactata tgagcatcat tcggatgtgg ctaggcgatg
     1021 atgtaaaatg taaggattac atcgttgccc tgcagcatcc cgtgaccact gacattaagc
     1081 attccataaa gatgtttgag ctaacactgg atgccctgat ctcgtttaac aagaggaccc
     1141 tagttctgtt tccaaatatc gatgcaggca gcaaggagat ggttcgagtg atgcggaaga
     1201 agggcatcga gcatcacccc aatttccgtg cagtcaagca cgtcccgttt gaccagttca
     1261 tacagctggt cgcccacgct ggctgcatga ttgggaatag cagctgcggc gtgcgagagg
     1321 ttggcgcttt cggaacaccc gtgatcaacc tgggcacaag gcagatagga agagaaaccg
     1381 gggagaatgt tcttcatgtc agggatgctg acacccaaga taaaatattg caagcactac
     1441 acctccagtt cggcaaacag tacccttgct caaagatata tggggatggg aatgctgttc
     1501 caaggatttt aaagtttctc aaatccattg accttcaaga gccactacag aagaaattct
     1561 gcttcccccc tgtaaaggag aacatctctc aagacattga ccacatcctg gaaactctga
     1621 gtgccttggc tgttgatctt ggcgggacaa acctgagggt ggcaatagtt agcatgaagg
     1681 gtgaaatcgt taagaagtac actcagttca accctaaaac ctatgaagaa aggattagtt
     1741 taatcctgca gatgtgtgtg gaagctgccg cggaagctgt gaaactcaat tgcagaattc
     1801 tgggagtagg catctccaca ggtggccgcg tgaatcccca ggaaggagtt gtgctgcatt
     1861 caaccaagct gatccaggaa tggaactccg tggacctcag gacacccctc tccgacaccc
     1921 tgcatctccc cgtgtgggtg gacaatgacg gcaactgtgc cgccatggca gagaggaagt
     1981 tcggccaagg aaaaggacag gagaacttcg tgacgctcat cacggggaca gggatcggtg
     2041 gggggatcat ccaccagcac gaactgatcc acggcagctc cttctgcgcg gcggagctcg
     2101 gccatctcgt ggtgtccctg gacggtcctg actgctcctg tggaagccat gggtgcatcg
     2161 aagcgtacgc ctctggaatg gccttgcaga gggaagcaaa gaaactccat gatgaggacc
     2221 tgctcttggt ggaagggatg tcagtaccaa aagacgaagc tgtgggtgcc ctccatctca
     2281 tccaggctgc caagctgggc aacgtgaagg cccagagcat cttacgaaca gctggaactg
     2341 ctttgggact tggggttgtg aacatcctcc acactatgaa tccttccctg gtgatcctgt
     2401 ctggagtcct ggccagtcac tacatccaca tcgtgaagga cgtcatccgc cagcaagcct
     2461 tgtcctccgt gcaggatgtg gacgtggtgg tctcagactt ggtggacccg gccctgcttg
     2521 gcgcagccag catggttctg gactacacaa cgcgcaggat ccactaggtc tcccgggaac
     2581 ggacacggac agagactcgg gagctgctta gagtggaacc atgctcttct agatcagtgt
     2641 ttctgcgaag gcaaatttgg ggggagggct gctgagacag ctcagtggtt aagagcctgc
     2701 cctgctcctg ccagtcccca gcacccatgt caggcagctc agctgcctgg aagccaagct
     2761 ccaggggacc caatgcctct ctgccggggg cagctgcact cagatgtaca tacccctctc
     2821 cacacacata caaataaagc ttatttttca aaaggcaaac ttgatatctg gtcccagaaa
     2881 taccagaatc tctctataaa agggattatc agaatggctc acaggctgtg gttcagctag
     2941 tccaacagtg gctgtcttgc aagggacagc ccaagagtcc tgttcagtgc ctagggctgg
     3001 atgtctcagc tggtcttcag tacacgtcag actcgtgcag caggctttaa taaccgtgaa
     3061 tggaaagcaa gggccaccag gcaatagcaa ttgcttcctt cttccttgta ctttacagaa
     3121 gctgtcaccg aaaggtgtgg ccccgattaa aggtggattt tcccacctca aaagatgtgg
     3181 atcaaaagtg gaccttccta cttcaaagga tttaattaaa aatctctccc aggtgtaccc
     3241 agcctcttag gctttagtta attccagatg taatcaagtt gacaaccaag aatagtatca
     3301 cacctttcaa atccactttc cccatgagct ttcgccaagg ctgctctgat agggtcatct
     3361 cactgcaaac cctgctgtgg gttccaggga cacttcctgc accaaaagga gcaatagcac
     3421 ctgacccaga agacttagga ctcctcatag acctcgggat gctagctagc atcatgggaa
     3481 ggcttagttt actagtcctt cctcagacca cagagagagc ctgtcacaca acagaagaca
     3541 agctcaccag aaatgcccac tcactttgat ttttttagca acataataca tttcacaact
     3601 tttcaagaag gcatactcct gttaagtagc caccaccagc caagggattg gacagatgtg
     3661 tcaccgcacc ccgggacagg acagatgtgt caccgcaccc ctgtgtctgc tgagagatgc
     3721 tttacttgag gagtttgtga gatactttag actggggtcc aagttatgac ggccttagtg
     3781 gctgtcctta tcttcccaga gattgcaggc aggcaggccg gcctcagagc ttctggaaag
     3841 aaagcctttc actgctctct gtgtcccaga atgtcagcat acacatttac gtgtactttg
     3901 tgacacctaa agcccagagc catggatctg gatttggact acattattgt ctttttgatg
     3961 tgtttgtttt cgagacagaa tctcgcttgt ctctgtaatc tcgcttggtt catcttaaac
     4021 ttacagcaat cctcctgccc ccgcttccag agtactggga ttataaacaa tcaatcacca
     4081 cacccagctt aacatctgct ttttcttgga tgacaaaata taaaagtcct cagttctgcc
     4141 taccgactgt accctggagt aagcacccac acgtgccagt gtatcagtta ccttctgctg
     4201 ctgtgatgga agtgaccaag gcaacttaca gaagggagag tttcattagc ttacagttct
     4261 agaggattca taaaggcagg gaaggcacag gaacaggaag ccagggcagg gagcttgaga
     4321 aaccacatct tcaacgacaa gccaacagca gcagaagtga atgcaaatag ggcaggccac
     4381 ggactcaccc agactgcacc agcaaacagc accactgagg agcacgtacc gacatacctg
     4441 agcctacaga gacattccca ttcaaactgc atccactcct gactcccata aactcatgga
     4501 cgtaccgcca tgcaaagcag tcagtcagtc caccattcaa gtccccggag cctaacagtc
     4561 tccatactaa gcactacgtt tcttctgaga cgtaagggga cctctcaacg acgacgtcct
     4621 ggtaaaccca caatggcaga gtatacattt tgcttccaaa gagagcagtg gaagcatggg
     4681 aataggggca tgaagaatgg ggcacggaag gaattaggga cacagtggga aagaggcagg
     4741 gagaactcag caggacaaac tccaaccctg cagcttcata cctatcgggg ccatgagtgt
     4801 caaagggctt agaggttcag ccctccagct ttgctgtctg tcgcctacat acttgccggt
     4861 cttgggctgg ttctatgcag ccctttaacc agtggttctc agcctcccta atgctataat
     4921 cctttactat agttccacgt atttattaat acaactcatg ttgtggtgac ccacaaccag
     4981 aaaattactt tgctgctatt ttgctaactg tacttctact gtaatgaatc ataatgtaca
     5041 tatgtgatat gcaggatatg atatatgccc atgaaaagga tcttccgacc ccacaaaggg
     5101 gcaacgaccc acaggatgag aactgctgcc gtcaatcatc acctgggtct ggttattatc
     5161 caggcctgga gcaaaggatg cccacgaagc ggtcatcact caccagtggg tcaccctgta
     5221 atcactgcaa ccaaacaact gcactgttac cacgctgtga gccatgtatt tgaaaggata
     5281 ataccaaact gaaaaccaaa tcctggtttt aatcaactct gtggaaagtg taatatattc
     5341 tgacaaaata tatatggtga aactgtctca atttgaataa atatttttat tgcaattctg
     5401 aaccaatttt aaaagaaaag atacacttcc ttgtccaagt cttacagtgt gcttaccatc
     5461 acctctgcca aaatcataaa atcatttctt ttcccaaaag agctctgtat ttgtggccct
     5521 gctttatcat tatctaattt atgttatcaa gtgccactgc tttctgaacc aagggaaaag
     5581 gagctattat aaaggaccat tattaaacta
//
