LOCUS       NM_010309               3779 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus GNAS complex locus (Gnas), transcript variant 1, mRNA.
ACCESSION   NM_010309
VERSION     NM_010309.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ben-Shaanan,T.L., Knopper,K., Duan,L., Liu,R., Taglinao,H., Xu,Y.,
            An,J., Plikus,M.V. and Cyster,J.G.
  TITLE     Dermal TRPV1 innervations engage a macrophage- and
            fibroblast-containing pathway to activate hair growth in mice
  JOURNAL   Dev Cell 59 (21), 2818-2833 (2024)
   PUBMED   38851191
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Liu,L., El,K., Dattaroy,D., Barella,L.F., Cui,Y., Gray,S.M.,
            Guedikian,C., Chen,M., Weinstein,L.S., Knuth,E., Jin,E.,
            Merrins,M.J., Roman,J., Kaestner,K.H., Doliba,N., Campbell,J.E. and
            Wess,J.
  TITLE     Intra-islet alpha-cell Gs signaling promotes glucagon release
  JOURNAL   Nat Commun 15 (1), 5129 (2024)
   PUBMED   38879678
  REMARK    Erratum:[Nat Commun. 2024 Jul 29;15(1):6383. doi:
            10.1038/s41467-024-50810-2.. PMID: 39075062]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3779)
  AUTHORS   Tang,C., Zhong,C., Zhu,J., Yuan,F., Yang,J., Xu,Y. and Ma,C.
  TITLE     GNAS mutations suppress cell invasion by activating MEG3 in growth
            hormone-secreting pituitary adenoma
  JOURNAL   Oncol Res 32 (6), 1079-1091 (2024)
   PUBMED   38827318
  REMARK    GeneRIF: GNAS mutations suppress cell invasion by activating MEG3
            in growth hormone-secreting pituitary adenoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3779)
  AUTHORS   Plagge,A., Isles,A.R., Gordon,E., Humby,T., Dean,W., Gritsch,S.,
            Fischer-Colbrie,R., Wilkinson,L.S. and Kelsey,G.
  TITLE     Imprinted Nesp55 influences behavioral reactivity to novel
            environments
  JOURNAL   Mol Cell Biol 25 (8), 3019-3026 (2005)
   PUBMED   15798190
  REMARK    GeneRIF: Maternally expressed Nesp55 plays a role in controlling
            exploratory behavior.
REFERENCE   5  (bases 1 to 3779)
  AUTHORS   Abramowitz,J., Grenet,D., Birnbaumer,M., Torres,H.N. and
            Birnbaumer,L.
  TITLE     XLalphas, the extra-long form of the alpha-subunit of the Gs G
            protein, is significantly longer than suspected, and so is its
            companion Alex
  JOURNAL   Proc Natl Acad Sci U S A 101 (22), 8366-8371 (2004)
   PUBMED   15148396
  REMARK    GeneRIF: Data report that the XL exon of Gsalpha is longer than
            presumed and contributes an additional 364 codons to XLalphas.
REFERENCE   6  (bases 1 to 3779)
  AUTHORS   Wroe,S.F., Kelsey,G., Skinner,J.A., Bodle,D., Ball,S.T.,
            Beechey,C.V., Peters,J. and Williamson,C.M.
  TITLE     An imprinted transcript, antisense to Nesp, adds complexity to the
            cluster of imprinted genes at the mouse Gnas locus
  JOURNAL   Proc Natl Acad Sci U S A 97 (7), 3342-3346 (2000)
   PUBMED   10716699
REFERENCE   7  (bases 1 to 3779)
  AUTHORS   Peters,J., Wroe,S.F., Wells,C.A., Miller,H.J., Bodle,D.,
            Beechey,C.V., Williamson,C.M. and Kelsey,G.
  TITLE     A cluster of oppositely imprinted transcripts at the Gnas locus in
            the distal imprinting region of mouse chromosome 2
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3830-3835 (1999)
   PUBMED   10097123
  REMARK    GeneRIF: Gnas gene exhibits a complex imprinted pattern, giving
            rise to transcripts that are maternally, paternally, or
            biallelically expressed.
REFERENCE   8  (bases 1 to 3779)
  AUTHORS   Tachibana,M., Wilcox,E., Yokotani,N., Schneider,M. and Fex,J.
  TITLE     Selective amplification and partial sequencing of cDNAs encoding G
            protein alpha subunits from cochlear tissues
  JOURNAL   Hear Res 62 (1), 82-88 (1992)
   PUBMED   1429253
REFERENCE   9  (bases 1 to 3779)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
REFERENCE   10 (bases 1 to 3779)
  AUTHORS   MacMillan,P.
  TITLE     Women of the world, unite
  JOURNAL   Nurs Times 75 (33), 1391-1392 (1979)
   PUBMED   257483
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL593857.10, AK132636.1 and
            BU516278.1.
            
            On Jun 20, 2015 this sequence version replaced NM_010309.3.
            
            Summary: This locus has a highly complex imprinted expression
            pattern. It gives rise to maternally, paternally, and biallelically
            expressed transcripts that are derived from four alternative
            promoters and 5' exons. Some transcripts contain a differentially
            methylated region (DMR) at their 5' exons, which is commonly found
            in imprinted genes and correlates with transcript expression. This
            gene has an antisense transcript. One of the transcripts produced
            from this locus, and the antisense transcript, are both paternally
            expressed noncoding RNAs, and may regulate imprinting in this
            region. In addition, one of the transcripts contains a second
            overlapping ORF, which encodes a structurally unrelated protein -
            Alex. Alternative splicing of downstream exons is also observed,
            which results in different forms of the stimulatory G-protein alpha
            subunit, a key element of the classical signal transduction pathway
            linking receptor-ligand interactions with the activation of
            adenylyl cyclase and a variety of cellular reponses. Additional
            transcript variants have been found for this gene, but the
            full-length nature and/or biological validity of some variants have
            not been determined. [provided by RefSeq, Jun 2015].
            
            Transcript Variant: This variant (1, also known as GNASXL) is
            paternally expressed. It includes an alternate 5' exon, compared to
            variant 7. This variant includes two overlapping open reading
            frames encoding distinct isoforms XLas and Alex, respectively. This
            RefSeq represents the isoform XLas (also known as alpha sXXL),
            which is a neuroendocrine-specific G-protein alpha subunit. It has
            a distinct and longer N-terminus, compared to isoform GNASL.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY519502.1, AY519501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 10097123, 11056047
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1978              AL593857.10        154018-155995
            1979-3754           AK132636.1         67-1842
            3755-3779           BU516278.1         566-590
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 97.89 cM"
     gene            1..3779
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="GNAS complex locus"
                     /db_xref="GeneID:14683"
                     /db_xref="MGI:MGI:95777"
     exon            1..2359
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     CDS             4..3405
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="isoform XLas is encoded by transcript variant 1;
                     protein ALEX; neuroendocrine secretory protein 55; GNAS
                     (guanine nucleotide binding protein, alpha stimulating)
                     complex locus; secretogranin VI; Gs alpha subunit;
                     stimulatory G-protein alpha subunit; extra large alphas
                     protein; alternative gene product encoded by XL-exon;
                     adenylate cyclase-stimulating G alpha protein"
                     /codon_start=1
                     /product="protein GNAS isoform XLas"
                     /protein_id="NP_034439.2"
                     /db_xref="CCDS:CCDS17151.1"
                     /db_xref="GeneID:14683"
                     /db_xref="MGI:MGI:95777"
                     /translation="MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAG
                     PAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPP
                     EEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPP
                     PEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDY
                     SPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPP
                     LWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIA
                     SDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPE
                     AMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEA
                     PAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEP
                     AAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARA
                     AYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPP
                     SPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCF
                     QWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEER
                     RKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQ
                     MRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPV
                     ELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCA
                     QYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRK
                     WIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFL
                     NKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRIS
                     TASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL"
     exon            2360..2432
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2433..2477
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2532
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2652
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2750
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2751..2805
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2879
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3059
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..3190
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3258
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3761
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3705..3710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: AATAAA"
     polyA_site      3735
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
     regulatory      3738..3743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: AATAAA"
     regulatory      3745..3750
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: ATTAAA"
     polyA_site      3756
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
     polyA_site      3761
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
ORIGIN      
        1 gttatgggca tgttcaactg cctccacggc aataatatgt caggacaaca cgatatcccc
       61 cctgaagtcg gggagcagcc cgagcaagaa cctttggaag ccccaggggc agctgccccc
      121 ggtgctgggg ctggcccagc cgaagaaatg gcgaccgaac cggactccga accgtctaac
      181 aatgagcccg tccccgacga gactggcagt gagatcagtg gacccccaga agactccaaa
      241 tctgacatcc aaagcccctg ccaggccttc gaggaagtcc gagtgggtgg agactacagc
      301 ccacctccgg aggaagccat gccattcgag acacaacagc ccagcctggg agatttctgg
      361 cccaccctgg agcagccagg accatctggg accccatcag gcctccaagc cttcaaccca
      421 gcgattttgg agcccgggac ccccactggc gcgagcccag gcctgggagc ctataccccc
      481 ccaccagaag aagctatgcc atttgagttc aacgagcctg cccagggaga ccatagccag
      541 cctcccttgc aagtcccaga ccttgcgcca ggaggtccgg aagcattggt ccccagagct
      601 cttcccgcgg agcccgggaa catcagattt gaaaacgctg gcttccgaga agactacagc
      661 cctccccctg aagaatctgt gccatttcag gtcggtggag aagaattcgg gggcgatagc
      721 ccacccccag gactcccgcg agtcatccca caaatcggca ttggcggcga gttcccgaca
      781 gtcgcggtcc cgagtgcgct ctgcctcgct cccgccgaga acgcgcctcc cctctgggtc
      841 cgaggcgcca ttgacagacc attccgcgag gctgtcagat ctcctcctaa cttcgcatgc
      901 gacagccccc cgatggagat caccagaccc ctgcttgaga ttggcagagc ctccattggg
      961 gtcgacgacg acaccgctgt caatatggac agccccccaa tcgcaagtga tggcccgccc
     1021 atcgaagtct cgggagcccc agataagagc gagtgcgcag agagaccccc agttgagcga
     1081 gaagcagccg agatggaagg aagccctacc accgccactg cggtggaagg aaaagtcccc
     1141 tctccggaga gaggggacgg atcttccacc cagcctgaag caatggatgc caagccagcc
     1201 cctgctgccc aagccgtctc taccggatct gatgctggag ctcctacgga ttccgcgatg
     1261 ctcacagata gccagagcga tgccggagaa gacgggacag ccccaggaac gccttcagat
     1321 ctccagtcgg atcctgaaga actcgaagaa gccccagctg tccgcgccga tcctgacgga
     1381 ggggcagccc cagtcgcccc agccactcct gccgagtccg agtctgaagg cagcagagat
     1441 ccagccgccg agccagcctc cgaggcagtc cctgccacca cggccgagtc tgcctccggg
     1501 gcagcccctg tcacccaggt ggagcccgca gccgcggcag tctctgccac cctggcggag
     1561 cctgccgccc gggcagcccc tatcaccccc aaggagccca ctacccgggc agtcccctct
     1621 gctagagccc atccggccgc tggagcagtc cctggcgccc cagcaatgtc agcctctgct
     1681 agggcagctg ccgctagggc agcctatgca ggtccactgg tctggggagc caggtcactc
     1741 tcagctactc ccgccgctcg ggcatccctt cctgcccgcg cagcagctgc cgcccgggca
     1801 gcctctgctg cccgcgcagt cgctgctggc cggtcagcct ctgccgcccc cagcagggcc
     1861 catcttagac cccccagccc cgagatccag gttgctgacc cgcctactcc gcggcctcct
     1921 ccgcggccga ctgcctggcc tgacaagtac gagcggggcc gaagctgctg caggtacgag
     1981 gcatcgtctg gcatctgcga gatcgagtcc tccagtgatg agtcggaaga aggggccacc
     2041 ggctgcttcc agtggcttct gcggcgaaac cgccgccctg gcctgccccg gagccacacg
     2101 gtcgggagca acccagtccg caacttcttc acccgagcct tcggaagctg cttcggtcta
     2161 tccgagtgta cccgatcacg atccctcagc cccgggaagg ccaaggatcc tatggaggag
     2221 aggcgcaaac agatgcgcaa agaagccatt gagatgcgag agcagaagcg cgcagataag
     2281 aaacgcagca agctcatcga caagcaactg gaggaggaga agatggacta catgtgtaca
     2341 caccgcctgc tgcttctagg tgctggagag tctggcaaaa gcaccattgt gaagcagatg
     2401 aggatcctgc atgttaatgg gtttaacgga gagggcggcg aagaggaccc gcaggctgca
     2461 aggagcaaca gcgatggtga gaaggccact aaagtgcagg acatcaaaaa caacctgaag
     2521 gaggccattg aaaccattgt ggccgccatg agcaacctgg tgccccctgt ggagctggcc
     2581 aaccctgaga accagttcag agtggactac attctgagcg tgatgaacgt gccgaacttt
     2641 gacttcccac ctgaattcta tgagcatgcc aaggctctgt gggaggatga gggagtgcgt
     2701 gcctgctacg agcgctccaa tgagtaccag ctgattgact gtgcccagta cttcctggac
     2761 aagattgatg tgatcaagca ggccgactac gtgccaagtg accaggacct gcttcgctgc
     2821 cgtgtcctga cctctggaat ctttgagacc aagttccagg tggacaaagt caacttccac
     2881 atgttcgatg tgggcggcca gcgcgatgag cgccgcaagt ggatccagtg cttcaatgat
     2941 gtgactgcca tcatcttcgt ggtggccagc agcagctaca acatggtcat tcgggaggac
     3001 aaccagacta accgcctgca ggaggctctg aacctcttca agagcatctg gaacaacaga
     3061 tggctgcgca ccatctctgt gattctcttc ctcaacaagc aagacctgct tgctgagaaa
     3121 gtcctcgctg gcaaatcgaa gattgaggac tactttccag agttcgctcg ctacaccact
     3181 cctgaggatg cgactcccga gccgggagag gacccacgcg tgacccgggc caagtacttc
     3241 attcgggatg agtttctgag aatcagcact gctagtggag atgggcgcca ctactgctac
     3301 cctcacttta cctgcgccgt ggacactgag aacatccgcc gtgtcttcaa cgactgccgt
     3361 gacatcatcc agcgcatgca tctccgccaa tacgagctgc tctaagaagg gaacacccaa
     3421 atttaattca gccttaagca caattaatta agagtgaaac gtaattgtac aagcagttgg
     3481 tcacccacca tagggcatga tcaacaccgc aacctttcct ttttccccca gtgattctga
     3541 aaaacccctc ttcccttcag cttgcttaga tgttccaaat ttagtaagct taaggcggcc
     3601 tacagaagaa aaagaaaaaa aaggccacaa aagttccctc tcactttcag taaataaaat
     3661 aaaagcagca acagaaataa agaaataaat gaaattcaaa atgaaataaa tattgtgttg
     3721 tgcagcatta aaaaatcaat aaaaattaaa aatgagcaaa aaaaaaaaaa aaaaaaaaa
//
