LOCUS       NM_001310083            3921 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus GNAS complex locus (Gnas), transcript variant 10,
            mRNA.
ACCESSION   NM_001310083 XM_006498775
VERSION     NM_001310083.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ben-Shaanan,T.L., Knopper,K., Duan,L., Liu,R., Taglinao,H., Xu,Y.,
            An,J., Plikus,M.V. and Cyster,J.G.
  TITLE     Dermal TRPV1 innervations engage a macrophage- and
            fibroblast-containing pathway to activate hair growth in mice
  JOURNAL   Dev Cell 59 (21), 2818-2833 (2024)
   PUBMED   38851191
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Liu,L., El,K., Dattaroy,D., Barella,L.F., Cui,Y., Gray,S.M.,
            Guedikian,C., Chen,M., Weinstein,L.S., Knuth,E., Jin,E.,
            Merrins,M.J., Roman,J., Kaestner,K.H., Doliba,N., Campbell,J.E. and
            Wess,J.
  TITLE     Intra-islet alpha-cell Gs signaling promotes glucagon release
  JOURNAL   Nat Commun 15 (1), 5129 (2024)
   PUBMED   38879678
  REMARK    Erratum:[Nat Commun. 2024 Jul 29;15(1):6383. doi:
            10.1038/s41467-024-50810-2.. PMID: 39075062]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3921)
  AUTHORS   Tang,C., Zhong,C., Zhu,J., Yuan,F., Yang,J., Xu,Y. and Ma,C.
  TITLE     GNAS mutations suppress cell invasion by activating MEG3 in growth
            hormone-secreting pituitary adenoma
  JOURNAL   Oncol Res 32 (6), 1079-1091 (2024)
   PUBMED   38827318
  REMARK    GeneRIF: GNAS mutations suppress cell invasion by activating MEG3
            in growth hormone-secreting pituitary adenoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3921)
  AUTHORS   Plagge,A., Isles,A.R., Gordon,E., Humby,T., Dean,W., Gritsch,S.,
            Fischer-Colbrie,R., Wilkinson,L.S. and Kelsey,G.
  TITLE     Imprinted Nesp55 influences behavioral reactivity to novel
            environments
  JOURNAL   Mol Cell Biol 25 (8), 3019-3026 (2005)
   PUBMED   15798190
  REMARK    GeneRIF: Maternally expressed Nesp55 plays a role in controlling
            exploratory behavior.
REFERENCE   5  (bases 1 to 3921)
  AUTHORS   Abramowitz,J., Grenet,D., Birnbaumer,M., Torres,H.N. and
            Birnbaumer,L.
  TITLE     XLalphas, the extra-long form of the alpha-subunit of the Gs G
            protein, is significantly longer than suspected, and so is its
            companion Alex
  JOURNAL   Proc Natl Acad Sci U S A 101 (22), 8366-8371 (2004)
   PUBMED   15148396
  REMARK    GeneRIF: Data report that the XL exon of Gsalpha is longer than
            presumed and contributes an additional 364 codons to XLalphas.
REFERENCE   6  (bases 1 to 3921)
  AUTHORS   Wroe,S.F., Kelsey,G., Skinner,J.A., Bodle,D., Ball,S.T.,
            Beechey,C.V., Peters,J. and Williamson,C.M.
  TITLE     An imprinted transcript, antisense to Nesp, adds complexity to the
            cluster of imprinted genes at the mouse Gnas locus
  JOURNAL   Proc Natl Acad Sci U S A 97 (7), 3342-3346 (2000)
   PUBMED   10716699
REFERENCE   7  (bases 1 to 3921)
  AUTHORS   Peters,J., Wroe,S.F., Wells,C.A., Miller,H.J., Bodle,D.,
            Beechey,C.V., Williamson,C.M. and Kelsey,G.
  TITLE     A cluster of oppositely imprinted transcripts at the Gnas locus in
            the distal imprinting region of mouse chromosome 2
  JOURNAL   Proc Natl Acad Sci U S A 96 (7), 3830-3835 (1999)
   PUBMED   10097123
  REMARK    GeneRIF: Gnas gene exhibits a complex imprinted pattern, giving
            rise to transcripts that are maternally, paternally, or
            biallelically expressed.
REFERENCE   8  (bases 1 to 3921)
  AUTHORS   Tachibana,M., Wilcox,E., Yokotani,N., Schneider,M. and Fex,J.
  TITLE     Selective amplification and partial sequencing of cDNAs encoding G
            protein alpha subunits from cochlear tissues
  JOURNAL   Hear Res 62 (1), 82-88 (1992)
   PUBMED   1429253
REFERENCE   9  (bases 1 to 3921)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
REFERENCE   10 (bases 1 to 3921)
  AUTHORS   MacMillan,P.
  TITLE     Women of the world, unite
  JOURNAL   Nurs Times 75 (33), 1391-1392 (1979)
   PUBMED   257483
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC062654.1, BM944582.1 and
            BU516278.1.
            
            On Jun 26, 2015 this sequence version replaced XM_006498775.1.
            
            Summary: This locus has a highly complex imprinted expression
            pattern. It gives rise to maternally, paternally, and biallelically
            expressed transcripts that are derived from four alternative
            promoters and 5' exons. Some transcripts contain a differentially
            methylated region (DMR) at their 5' exons, which is commonly found
            in imprinted genes and correlates with transcript expression. This
            gene has an antisense transcript. One of the transcripts produced
            from this locus, and the antisense transcript, are both paternally
            expressed noncoding RNAs, and may regulate imprinting in this
            region. In addition, one of the transcripts contains a second
            overlapping ORF, which encodes a structurally unrelated protein -
            Alex. Alternative splicing of downstream exons is also observed,
            which results in different forms of the stimulatory G-protein alpha
            subunit, a key element of the classical signal transduction pathway
            linking receptor-ligand interactions with the activation of
            adenylyl cyclase and a variety of cellular reponses. Additional
            transcript variants have been found for this gene, but the
            full-length nature and/or biological validity of some variants have
            not been determined. [provided by RefSeq, Jun 2015].
            
            Transcript Variant: This variant (10) differs in the 5' UTR,
            compared to variant 7. Variants 7 and 10 encode the same isoform
            guanine nucleotide binding protein alpha-s long (GNASL, also known
            as alpha-S2), a form of the G-protein alpha subunit.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062654.1, SRR7652917.160355.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene :: PMID: 10097123, 11056047
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-937               BC062654.1         1-937
            938-938             BM944582.1         382-382
            939-3899            BC062654.1         939-3899
            3900-3921           BU516278.1         569-590
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 97.89 cM"
     gene            1..3921
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="GNAS complex locus"
                     /db_xref="GeneID:14683"
                     /db_xref="MGI:MGI:95777"
     exon            1..174
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            175..2501
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     CDS             2363..3547
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="isoform GNASL is encoded by transcript variant 10;
                     protein ALEX; neuroendocrine secretory protein 55; GNAS
                     (guanine nucleotide binding protein, alpha stimulating)
                     complex locus; secretogranin VI; Gs alpha subunit;
                     stimulatory G-protein alpha subunit; extra large alphas
                     protein; alternative gene product encoded by XL-exon;
                     adenylate cyclase-stimulating G alpha protein"
                     /codon_start=1
                     /product="protein GNAS isoform GNASL"
                     /protein_id="NP_001297012.1"
                     /db_xref="CCDS:CCDS38356.1"
                     /db_xref="GeneID:14683"
                     /db_xref="MGI:MGI:95777"
                     /translation="MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLL
                     LLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEA
                     IETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVR
                     ACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVN
                     FHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSI
                     WNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRV
                     TRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYE
                     LL"
     exon            2502..2574
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2619
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2674
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2794
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2795..2892
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2893..2947
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3021
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3022..3201
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3332
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3400
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     exon            3401..3903
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3847..3852
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: AATAAA"
     polyA_site      3877
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
     regulatory      3880..3885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: AATAAA"
     regulatory      3887..3892
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="hexamer: ATTAAA"
     polyA_site      3898
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
     polyA_site      3903
                     /gene="Gnas"
                     /gene_synonym="5530400H20Rik; A930027G11Rik;
                     C130027O20Rik; Galphas; Gnas1; Gnasxl; GPSA; Gs-alpha;
                     Gsa; GSP; Nesp; Nesp55; Nespl; Oed-Sml; Oedsml; P1; P2;
                     P3; PHP1A; PHP1B; POH; SCG6"
                     /note="major polyA site"
ORIGIN      
        1 caccgcggag cgggttgctt caggtggctg gtaccagcgg cgctcgctag ctcccggctc
       61 tctgggctcg gggctgcagc accggctgga gcgaaccctt gtcccagcgc ttggcggcgg
      121 caggcagacg ggcaggcagg cgctgctttg cgtgtgagtg cgtctcactc acatagctaa
      181 cccaaggagc acctaagggt aatagcaaac tcttgtcatc tgcacctttg cactttcctt
      241 tctcttgtgt tatttttttt attttttttg tcttttctag actctcttcc cccctttttg
      301 agttgttggg cagttcttat ttcttgcttg cttttcggtt tcttgctgtt gttgggtgtt
      361 ttttttgttt gtttgtttgt ttgtttgttc tcgctttttt tgtttgctca tcctatttga
      421 cgctaactct taggcagcca gtccatcagc cccctacgaa gcccgggaag ccgcactcgg
      481 tgaccccaag acagcacagc ggagactgca gctgagcccc ccgacacggg gcgcacagga
      541 gccgggaagc ccgaggccgg gggcaagcag accgcccgag ccaggcgcga accaagcacc
      601 ccgaggtggc cggggcacca tgctgaagat ggccatgaag ctcaaagccc gagcggccga
      661 gagcgagagg aagacagccg cggctgccgc cgaagtggct gccgaagctg ccgtggcggc
      721 tgcggtgctg gcagaaccgt tcgcttcgca gaagaacggg atccccgcga agcccccgaa
      781 gtccgtcagg gtcctcgtga acgccgggcg cgatgtgccg cccgcaaagt tgctgaccga
      841 caaagagcgc aagaagatgg agaagaagct gctggagcag aggagaaagg aggagaagaa
      901 gagagagaaa gagaggaaga agttggagaa gatgttgaag aagaagaaga agaagaatgt
      961 gccagaagct ccgccagagg cccgaggccc cgagggcgcc gccgccaccg ccgcaggcga
     1021 agacgagggc gccgcgcagg gcgccgccgg gttcggagag ggcgccgcca ccgggggcgc
     1081 gagtagggcc tgggccgaat gggacagtga cactgatgaa gaagatgatg cctactacgc
     1141 gcctccccgc gtggggggcg tcgtgtggcg gcctgggcct gccaaggcag gcgcactcag
     1201 cgcgcacatg cccctcgtgg tgttcctggt gttctcggtt cgcagcccct catgggtgct
     1261 taacttcctg ctacagaggc gcacagagca gggccgcggc tgcggcttcg tgttcggggc
     1321 gacctgctgc ttcagagtgt tcctgggctg cgccttcttc tccttcctga gtggtcgcgc
     1381 caagcgcaga ggccgtgggc gatcccgggc caggggcacc gggcgccggg ggcgcgacga
     1441 gcggctcatc tagtcagcct cccccccacc cctccaccat catcatctac atcgccatcg
     1501 accaccacca ccaacaccac caacacatcc accaccacca ccttccacca ccattgctgt
     1561 gtagtgtgga tctttattag tgcttgtgtg tggacacatt ttcctttttg gttgctctgt
     1621 cctatgctta gtgctctcct agcttttctc caagctccct gctttctggc tctctcagtg
     1681 tctccctctc tctctctctc tctccttttc gaaattttcc taagtccggg cccgcactcc
     1741 ctccctttcc gcccaccccg gccccttggc ggcgcctgcc cgccctgctg ctgcaggagg
     1801 agggggatcg ggggccgccc ggcccggggg tggagcgctg gcatcgtgtg aggggtcgtc
     1861 accaccgcgg ggccgccccc ttttcccccc gccgcacggc caggccccgg ccccgaactg
     1921 cgggtggggc gagggggcga cccctccgtg gagccggccc gagccggacg tgggtgctgg
     1981 gacccctgcc cggcctctgg gcaccgcctc ccggcgcccg cgcccgccct cccagccgcg
     2041 gccctcctcc tcctcctctt cttcccaccc ccctcgccgc cgtcgcctcc gcctcccccc
     2101 gctcccccgc ccgccgcggc gcttttgggt cggggcggcg ggcccgggcc gaggcaataa
     2161 gagcggcggc ggcggcggcg gcggcagcag ctcccgcggc tcctgctctg ctccgcctcg
     2221 gccccggagc gaggggcgga gagccgcgcg cttgccttag tccgagccgc caccctcccc
     2281 gcgttccccg ctttcccggc ccgcccgagg ccgcccgccc cgtccccgcc gccccgcagc
     2341 ccggccgcgc cccgccgccg ccatgggctg cctcggcaac agtaagaccg aggaccagcg
     2401 caacgaggag aaggcgcagc gcgaggccaa caaaaagatc gagaagcagc tgcagaagga
     2461 caagcaggtc taccgggcca cgcaccgcct gctgctgctg ggtgctggag agtctggcaa
     2521 aagcaccatt gtgaagcaga tgaggatcct gcatgttaat gggtttaacg gagagggcgg
     2581 cgaagaggac ccgcaggctg caaggagcaa cagcgatggt gagaaggcca ctaaagtgca
     2641 ggacatcaaa aacaacctga aggaggccat tgaaaccatt gtggccgcca tgagcaacct
     2701 ggtgccccct gtggagctgg ccaaccctga gaaccagttc agagtggact acattctgag
     2761 cgtgatgaac gtgccgaact ttgacttccc acctgaattc tatgagcatg ccaaggctct
     2821 gtgggaggat gagggagtgc gtgcctgcta cgagcgctcc aatgagtacc agctgattga
     2881 ctgtgcccag tacttcctgg acaagattga tgtgatcaag caggccgact acgtgccaag
     2941 tgaccaggac ctgcttcgct gccgtgtcct gacctctgga atctttgaga ccaagttcca
     3001 ggtggacaaa gtcaacttcc acatgttcga tgtgggcggc cagcgcgatg agcgccgcaa
     3061 gtggatccag tgcttcaatg atgtgactgc catcatcttc gtggtggcca gcagcagcta
     3121 caacatggtc attcgggagg acaaccagac taaccgcctg caggaggctc tgaacctctt
     3181 caagagcatc tggaacaaca gatggctgcg caccatctct gtgattctct tcctcaacaa
     3241 gcaagacctg cttgctgaga aagtcctcgc tggcaaatcg aagattgagg actactttcc
     3301 agagttcgct cgctacacca ctcctgagga tgcgactccc gagccgggag aggacccacg
     3361 cgtgacccgg gccaagtact tcattcggga tgagtttctg agaatcagca ctgctagtgg
     3421 agatgggcgc cactactgct accctcactt tacctgcgcc gtggacactg agaacatccg
     3481 ccgtgtcttc aacgactgcc gtgacatcat ccagcgcatg catctccgcc aatacgagct
     3541 gctctaagaa gggaacaccc aaatttaatt cagccttaag cacaattaat taagagtgaa
     3601 acgtaattgt acaagcagtt ggtcacccac catagggcat gatcaacacc gcaacctttc
     3661 ctttttcccc cagtgattct gaaaaacccc tcttcccttc agcttgctta gatgttccaa
     3721 atttagtaag cttaaggcgg cctacagaag aaaaagaaaa aaaaggccac aaaagttccc
     3781 tctcactttc agtaaataaa ataaaagcag caacagaaat aaagaaataa atgaaattca
     3841 aaatgaaata aatattgtgt tgtgcagcat taaaaaatca ataaaaatta aaaatgagca
     3901 aaaaaaaaaa aaaaaaaaaa a
//
