LOCUS       NM_001113384            6255 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus guanine nucleotide binding protein, alpha O (Gnao1),
            transcript variant B, mRNA.
ACCESSION   NM_001113384
VERSION     NM_001113384.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Roy,A.J., Leipprandt,J.R., Patterson,J.R., Stoll,A.C., Kemp,C.J.,
            Oula,Z.D., Mola,T., Batista,A.R., Sortwell,C.E., Sena-Esteves,M.
            and Neubig,R.R.
  TITLE     AAV9-Mediated Intrastriatal Delivery of GNAO1 Reduces
            Hyperlocomotion in Gnao1 Heterozygous R209H Mutant Mice
  JOURNAL   J Pharmacol Exp Ther 390 (2), 250-259 (2024)
   PUBMED   38866563
  REMARK    GeneRIF: AAV9-Mediated Intrastriatal Delivery of GNAO1 Reduces
            Hyperlocomotion in Gnao1 Heterozygous R209H Mutant Mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Jarysta,A., Tadenev,A.L.D., Day,M., Krawchuk,B., Low,B.E.,
            Wiles,M.V. and Tarchini,B.
  TITLE     Inhibitory G proteins play multiple roles to polarize sensory hair
            cell morphogenesis
  JOURNAL   Elife 12, 88186 (2024)
   PUBMED   38651641
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6255)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6255)
  AUTHORS   Xu,J., Peng,Q., Cai,J., Shangguan,J., Su,W., Chen,G., Sun,H.,
            Zhu,C. and Gu,Y.
  TITLE     The Schwann cell-specific G-protein Galphao (Gnao1) is a
            cell-intrinsic controller contributing to the regulation of
            myelination in peripheral nerve system
  JOURNAL   Acta Neuropathol Commun 12 (1), 24 (2024)
   PUBMED   38331815
  REMARK    GeneRIF: The Schwann cell-specific G-protein Galphao (Gnao1) is a
            cell-intrinsic controller contributing to the regulation of
            myelination in peripheral nerve system.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6255)
  AUTHORS   Polikarpova,A.V., Egorova,T.V., Lunev,E.A., Tsitrina,A.A.,
            Vassilieva,S.G., Savchenko,I.M., Silaeva,Y.Y., Deykin,A.V. and
            Bardina,M.V.
  TITLE     CRISPR/Cas9-generated mouse model with humanizing single-base
            substitution in the Gnao1 for safety studies of RNA therapeutics
  JOURNAL   Front Genome Ed 5, 1034720 (2023)
   PUBMED   37077890
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6255)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
REFERENCE   7  (bases 1 to 6255)
  AUTHORS   Strathmann,M., Wilkie,T.M. and Simon,M.I.
  TITLE     Alternative splicing produces transcripts encoding two forms of the
            alpha subunit of GTP-binding protein Go
  JOURNAL   Proc Natl Acad Sci U S A 87 (17), 6477-6481 (1990)
   PUBMED   1697681
REFERENCE   8  (bases 1 to 6255)
  AUTHORS   Strathmann,M., Wilkie,T.M. and Simon,M.I.
  TITLE     Diversity of the G-protein family: sequences from five additional
            alpha subunits in the mouse
  JOURNAL   Proc Natl Acad Sci U S A 86 (19), 7407-7409 (1989)
   PUBMED   2508088
REFERENCE   9  (bases 1 to 6255)
  AUTHORS   Jones,D.T. and Reed,R.R.
  TITLE     Molecular cloning of five GTP-binding protein cDNA species from rat
            olfactory neuroepithelium
  JOURNAL   J Biol Chem 262 (29), 14241-14249 (1987)
   PUBMED   2820999
REFERENCE   10 (bases 1 to 6255)
  AUTHORS   Itoh,H., Kozasa,T., Nagata,S., Nakamura,S., Katada,T., Ui,M.,
            Iwai,S., Ohtsuka,E., Kawasaki,H., Suzuki,K. et al.
  TITLE     Molecular cloning and sequence determination of cDNAs for alpha
            subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go
            from rat brain
  JOURNAL   Proc Natl Acad Sci U S A 83 (11), 3776-3780 (1986)
   PUBMED   3086867
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102398.12 and AC138118.4.
            
            On Dec 14, 2022 this sequence version replaced NM_001113384.1.
            
            Transcript Variant: This variant (B) includes alternate exons in
            the 3' coding region, compared to variant a, resulting in isoform b
            (also known as G0B-alpha) that has a distinct C-terminus, compared
            to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219379.11994.1, M36778.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-869               AC102398.12        116176-117044       c
            870-912             AC102398.12        115936-115978       c
            913-1054            AC102398.12        31257-31398         c
            1055-1215           AC138118.4         133361-133521       c
            1216-1344           AC138118.4         128187-128315       c
            1345-1474           AC138118.4         127288-127417       c
            1475-1628           AC138118.4         123591-123744       c
            1629-6255           AC138118.4         117028-121654       c
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 45.94 cM"
     gene            1..6255
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="guanine nucleotide binding protein, alpha O"
                     /db_xref="GeneID:14681"
                     /db_xref="MGI:MGI:95775"
     exon            1..869
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     CDS             752..1816
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="isoform B is encoded by transcript variant B;
                     guanine nucleotide-binding protein G(o) subunit alpha;
                     GTP-binding protein alpha-o subunit; Go alpha;
                     heterotrimeric guanine nucleotide-binding protein 1g"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein G(o) subunit
                     alpha isoform B"
                     /protein_id="NP_001106855.1"
                     /db_xref="CCDS:CCDS85585.1"
                     /db_xref="GeneID:14681"
                     /db_xref="MGI:MGI:95775"
                     /translation="MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGES
                     GKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKT
                     DSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSL
                     DRIGAGDYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFED
                     VTAIIFCVALSGYDQVLHEDETTNRMHESLKLFDSICNNKWFTDTSIILFLNKKDIFE
                     EKIKKSPLTICFPEYTGPSAFTEAVAHIQGQYESKNKSAHKEVYSHVTCATDTNNIQF
                     VFDAVTDVIIAKNLRGCGLY"
     exon            870..912
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1054
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1215
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1344
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1474
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1628
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..6255
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6166..6171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="hexamer: AATAAA"
     polyA_site      6190
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="major polyA site"
     regulatory      6232..6237
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="hexamer: AATAAA"
     polyA_site      6255
                     /gene="Gnao1"
                     /gene_synonym="alphaO; Galphao; Gnao; Hg1g"
                     /note="major polyA site"
ORIGIN      
        1 ctgcccgcct cttctcgcgc tccccgctgg ccgcggctcc ctcgcgctct ccccgtctcc
       61 tgtccgctcc gccgagcgat gcgagttctg ggccccggcg acgccgcctc ccgctagaga
      121 gagctgcacc ccccaccccc ggcccggccc tctgcccagc cctgccccgc gcgcgggggt
      181 cggagaaggc gccgcggacg catcgacggc cgaggagcgg cgatgcacat gcactagcgg
      241 caccccctaa ctcactccct ccacaccccg cgccaccgcc gcctccacct tctccgcctc
      301 cgcctcctcc tcctccgcct ccggcagccg cggcagaagg acccaccctg ccccccaccc
      361 caccctccgc cggctccggc tgcggatcca gcctcgactc ctattttatt tattttgggt
      421 cgtgcactag cctcggtgcc tgcaacccgc gcctcccggg cccgcgggcg cctcctctct
      481 cggctccgga gccccagacc ccggctaccc tcacctcgac acccccagac cccagccagc
      541 cgccgctaat cttcgccgct ggaatcttaa tagaggctgt ccttttgggg ggattctggt
      601 ctttcgacaa ttttgttccc agccaaggag aggatatcgt gattttctcc cctttgagcc
      661 caggctctgc tctgtggggg ggtggggggc gcgccgaccc ggggagtcgt gccagcctag
      721 tcgtgcgggc tgtggcaggg aaggggccac catgggatgt acgctgagcg cagaggagag
      781 agccgccctc gagcggagca aggcgattga gaaaaacctc aaagaagatg gcatcagcgc
      841 cgccaaagac gtgaaattac tcctgctggg ggctggagaa tcaggaaaaa gcaccattgt
      901 gaagcagatg aagatcatcc atgaagatgg cttctctggg gaagacgtga agcagtacaa
      961 gcctgtggtc tacagcaaca ccatccagtc tctggcggcc attgtccggg ccatggacac
     1021 tttgggcgtg gagtatggtg acaaggagag gaagacggac tccaagatgg tgtgtgacgt
     1081 ggtgagtcgt atggaagaca ctgaaccgtt ctctgcagaa cttctttctg ccatgatgcg
     1141 actctggggc gactcgggga tccaggagtg cttcaaccga tctcgggagt atcagctcaa
     1201 tgactctgcc aaatactacc tggacagcct ggatcggatt ggagccggtg actaccagcc
     1261 cactgagcag gacatcctcc gaaccagagt caaaacaact ggcatcgtag aaacccactt
     1321 caccttcaag aacctccact tcaggctgtt tgacgtcggg ggccagcgat ctgaacgcaa
     1381 gaagtggatc cactgctttg aggatgtcac ggccatcatc ttctgtgtcg cactcagcgg
     1441 ctatgaccag gtgctccacg aggacgaaac cacgaaccgc atgcacgaat ccctgaagct
     1501 cttcgacagc atctgcaaca acaagtggtt cacagacaca tctattatcc tgtttctcaa
     1561 caagaaggac atatttgagg agaagatcaa gaagtcccca ctcaccatct gctttcctga
     1621 atacacaggc cccagtgcct tcacagaagc tgtggctcac atccaagggc agtatgagag
     1681 taagaataag tcagctcaca aggaagtcta cagccatgtc acctgtgcca cggacaccaa
     1741 caacatccaa ttcgtctttg atgccgtgac agatgtcatc atcgccaaaa acctacgggg
     1801 ctgtggactc tactgagccc tggcctccta cccagcctgc cactcactcc tcccctggac
     1861 ccagagctct gtcactgctc agatgccctg ttaactgaag aaaacctgga ggctagcctt
     1921 gggggcagga ggaggcatcc tttgagcatc cccaccccac ccaacttcag cctcgtgaca
     1981 cgtgggaaca gggttgggca gaggtgtgga acagcacaag gccagagacc acggcatgcc
     2041 acttgggtgc tgctcactgg tcagctgtgt gtcttacaca gaggccgagt gggcaacact
     2101 gccatctgat tcagaatggg catgccctgt cctctgtacc tcttgttcag tgtcctggtt
     2161 tctcttccac cttggtgata ggatggctgg caggaaggcc ccatggaagg tgctgcttga
     2221 ttaggggata gtcgatggca tctctcagca gtcctcaggg tctgtttggt agagggtggt
     2281 ttcgtcgaca aaagccaaca tggaatcagg ccacttttgg ggcgcaaaga ctcagacttt
     2341 ggggacgggt tccctcctcc ttcactttgg atcttggccc ctctctggtc atcttccctt
     2401 gcccttgggc tccccaggat actcagccct gactcccatg gggttgggaa tattccttaa
     2461 gactggctga ctgcaaaggt caccgatgga gaaacatccc tgtgctacag aattgggggt
     2521 gggacagctg agggggcagg cggctctttc ctgatagttg atgacaagcc ctgagaatgc
     2581 catctgctgg ctccactcac acgggctcaa ctgtcctggg tgatagtgac ttgccaggcc
     2641 acaggctgca ggtcacagac agagcaggca agcagccttg caactgcaga ttacttaggg
     2701 agaagcatcg gggcctcgtg agccaggccc cgtagccagt gccctgcttt actccagcct
     2761 tggtcaggaa gtcgaaagcc cttggtgtat tcctggtctc ggagcaaata atgagccagc
     2821 accctgaagg gtgggctcca actcagacat gcagccagcc ccctaggtgg gtaaacgccc
     2881 tagggaccta gggagagcct ttgctgcaga gattcctaag caaaacggcg tggtggagct
     2941 ttggcaaccc tagccccagc taactttgga cagtcagcat atgtccctgc catccctaga
     3001 catctccagt cagctggtat cacagccagt ggttcagaca ggtttgaatg ctcatgtggc
     3061 aggctgtaca gatgcagatg tgcccctggg cagtcgggaa aggtctacgg gtctctgccg
     3121 ttctcaagcc ctgtattttc ctgccctcca ctctgaaccc tgctccgtca caggaccctg
     3181 gccctctttc atagtccctg tgaccttgcc accagctccg gcctctttgg ttccaacaag
     3241 aacagaactg tgggtagtct agggaagcat gggaccttct ccaatgccaa atgcttgtag
     3301 gggtcctctg ggagttcact ccccatcttg gtgatcctct ggggagccca ccccagtctg
     3361 gttgcctcac ccttccccct ctacccatat ttttctacat ctccatagtt ctaggaccct
     3421 gtgccatgta ctggggtaac agagaacagc ctggagacca cgatctcagt gatggcctcg
     3481 tgtgatcaaa aagaccagtt cagaccctgg gccaacagca gctatttctc agcagcaccc
     3541 ctgggtgtgg ccacctgtgt cgacaggctg taaccccaag tttaaaagca cctacggtca
     3601 agggttgttg gccaagcccc atcttttagg accatttcca ggaaggtaga aatcagcatc
     3661 cctaacaatc cctctatggc tcccattccc caagtggccc ctccaaatca ttgcagcaca
     3721 gttgtcagag ctcacagcta gaatcagggc ctgtacccag cctccccact cctgtcccca
     3781 agacttcctt ctgctcgacc tactgtgttc agcctagtgt catgcactac tgtcagcctc
     3841 aggtagcgag gtctcaggct gtctactcta agccttcaag aacatggtcc acagggaagg
     3901 ctgttctgtg tcccacccat gtcgtcacca gcagctctac catggggggg acaagcatgt
     3961 gtgacaggtg aatcttggct ccgccgtggc tttgggtatg ggggaagtgt tgaaagtgac
     4021 cagatcaggg gaatggccca tatgtgcagt ggccttgtcc ccagaggggc catgctgtag
     4081 tgtctgagta gtagtataac tcagcaggct aggagccagt tcccaggaag agtgccttca
     4141 ctgtattcgt ggaaccgtgc ctgttgccta ggtgcctgtg ggcaaatcct gtgccctgtg
     4201 ccaattacca ttctaagaat ctgcccttta agcataggta gaggcttctg agcctctggg
     4261 ctgagctgta gagggtgctg gcggactctg ggctgagccg tggagggtgc tggcggactc
     4321 tgggctgagc cgtggagggt gctggtggac tctgggctga gccgtggagg gtgctggtgg
     4381 actctgggct gagccgtgga gggtgctggc gaactctggg ctgagccgtg gagggtgctg
     4441 gtggactcta ggctgagctg tggagggtgc tggcagactc tgggctgagc cgtggagggt
     4501 gctggtggac tctgggctga gctgtggagg gtgctggcag actctgggct gagctgtgga
     4561 gggtgctggc agactctggg ctgagctgtg gagggtgctg gtggactctg ggctgagccg
     4621 tggagggtgc tggcggactc taggctgagc tgtggagggt gctggcagac tctgggctga
     4681 gccgtggagg gtgctggtgg actctaggct gagctgtgga gggtgctggc agactctggg
     4741 ctgagctgtg gagggtgctg gtggactctg ggctgagccg tggagggtgc tggcggactc
     4801 taggctgagc tgtggagggt gctggcagac tctgggctga gctgtggagg gtgctggcgg
     4861 actctgggct gagctgtgga gggtgctggt ggactctggt gtgggaacgg ggatggattc
     4921 ctcccagtct gatccactca ttagtagaaa tggccttcag cctctcacga tcccttctgt
     4981 ggatggagcc cctagatcct ggtgccctgt ccccttgtcc cctcttgtac catgcagagc
     5041 accaccatca tctcagcctt gcagctcagg tccacaccct gctgttggga actggaggca
     5101 tctgacctgt gtggtctgta ctggatatca ctgagtgccc tgttcaaccc catacccagg
     5161 ctgtctctca ggttgacagc gcttcacccc agcccttgcc ctatgcagaa agtactgctt
     5221 cgagagatgt acctgtggtt cccacgtact acatgctaca gccagtgacc tgagggcagg
     5281 gttcacctca cactcctcct gaccctctcc ccatctccat ctgacccagc ctcttgacac
     5341 agagatccag cacacctagg agagactcat gaaccctcct gacccagtag tagcctgtca
     5401 ccttcttctg aagatcctgg gggcacagaa catcttctaa ctgccacccc ttttttctgg
     5461 gcctcccggt aagctccagg tatagctcca cagctctgag gaatccagtc ctgaggctcc
     5521 acctccactg taattgagct cctggaggca agcaccttcc aggctgctga tcccatcagg
     5581 ctacaagcta aagaaagaaa gttccagcat cccaaagaac agggccaggg gtgtaagcag
     5641 gcttccccag gccctaccac tcaggagagc cgtgtactca tctgcttaaa gagtggggtt
     5701 caggctcaca gatgccaccc catgccagat gatggccatg tcaaggaggg aacaggtaga
     5761 agaaaaccag aaataatggc cccatccaaa gtcctgagcc ctctaggcag agtgcggtac
     5821 cctatgacag gcagcttcct tctcttcccc aggcacaaaa ggccatcagc agaaacagta
     5881 cccttcccag cagcccccag gccctctcct cccctaagcc tcctgcacca gccacagttg
     5941 tctgcccagc atggacagca gcccccaact cctgttgttc tccctgactt ctgacttgac
     6001 ccctgtggct gatgcacaca cacccttcaa gtcccaggcc agaggctggg caccccacgt
     6061 tcctcttagc agagccttct taggtgcacc cgccccacct cttgacccac caccaccacc
     6121 taccctcatt aactgtaatt ttgtaagaag agtgatttgc tggttaataa atacctagtt
     6181 attgtaataa tttattggct gccataatgt atttattagt cacccaccat taataaaaag
     6241 tgccagctct ttcta
//
