LOCUS       NM_010305               3193 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus G protein subunit alpha i1 (Gnai1), mRNA.
ACCESSION   NM_010305 XM_355574
VERSION     NM_010305.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Hartig,E.I., Day,M., Jarysta,A. and Tarchini,B.
  TITLE     Proteins required for stereocilia elongation during mammalian hair
            cell development ensure precise and steady heights during adult
            life
  JOURNAL   Proc Natl Acad Sci U S A 121 (40), e2405455121 (2024)
   PUBMED   39320919
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Jarysta,A., Tadenev,A.L.D., Day,M., Krawchuk,B., Low,B.E.,
            Wiles,M.V. and Tarchini,B.
  TITLE     Inhibitory G proteins play multiple roles to polarize sensory hair
            cell morphogenesis
  JOURNAL   Elife 12, 88186 (2024)
   PUBMED   38651641
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3193)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3193)
  AUTHORS   Pulcrano,S., De Gregorio,R., De Sanctis,C., Volpicelli,F.,
            Piscitelli,R.M., Speranza,L., Perrone-Capano,C., di Porzio,U.,
            Caiazzo,M., Martini,A., Giacomet,C., Medina,D., Awatramani,R.,
            Viggiano,D., Federici,M., Mercuri,N.B., Guatteo,E. and
            Bellenchi,G.C.
  TITLE     miR-218 Promotes Dopaminergic Differentiation and Controls Neuron
            Excitability and Neurotransmitter Release through the Regulation of
            a Synaptic-Related Genes Network
  JOURNAL   J Neurosci 43 (48), 8104-8125 (2023)
   PUBMED   37816598
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3193)
  AUTHORS   Li,Y., Chai,J.L., Shi,X., Feng,Y., Li,J.J., Zhou,L.N., Cao,C. and
            Li,K.R.
  TITLE     Galphai1/3 mediate Netrin-1-CD146-activated signaling and
            angiogenesis
  JOURNAL   Theranostics 13 (7), 2319-2336 (2023)
   PUBMED   37153740
  REMARK    GeneRIF: Galphai1/3 mediate Netrin-1-CD146-activated signaling and
            angiogenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3193)
  AUTHORS   Wilkie,T.M., Gilbert,D.J., Olsen,A.S., Chen,X.N., Amatruda,T.T.,
            Korenberg,J.R., Trask,B.J., de Jong,P., Reed,R.R., Simon,M.I. et
            al.
  TITLE     Evolution of the mammalian G protein alpha subunit multigene family
  JOURNAL   Nat Genet 1 (2), 85-91 (1992)
   PUBMED   1302014
  REMARK    Review article
REFERENCE   7  (bases 1 to 3193)
  AUTHORS   Strathmann,M., Wilkie,T.M. and Simon,M.I.
  TITLE     Diversity of the G-protein family: sequences from five additional
            alpha subunits in the mouse
  JOURNAL   Proc Natl Acad Sci U S A 86 (19), 7407-7409 (1989)
   PUBMED   2508088
REFERENCE   8  (bases 1 to 3193)
  AUTHORS   Blatt,C., Eversole-Cire,P., Cohn,V.H., Zollman,S., Fournier,R.E.,
            Mohandas,L.T., Nesbitt,M., Lugo,T., Jones,D.T., Reed,R.R. et al.
  TITLE     Chromosomal localization of genes encoding guanine
            nucleotide-binding protein subunits in mouse and human
  JOURNAL   Proc Natl Acad Sci U S A 85 (20), 7642-7646 (1988)
   PUBMED   2902634
REFERENCE   9  (bases 1 to 3193)
  AUTHORS   Jones,D.T. and Reed,R.R.
  TITLE     Molecular cloning of five GTP-binding protein cDNA species from rat
            olfactory neuroepithelium
  JOURNAL   J Biol Chem 262 (29), 14241-14249 (1987)
   PUBMED   2820999
REFERENCE   10 (bases 1 to 3193)
  AUTHORS   Sullivan,K.A., Liao,Y.C., Alborzi,A., Beiderman,B., Chang,F.H.,
            Masters,S.B., Levinson,A.D. and Bourne,H.R.
  TITLE     Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA
            and amino acid sequences of the alpha chains
  JOURNAL   Proc Natl Acad Sci U S A 83 (18), 6687-6691 (1986)
   PUBMED   3092218
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY740128.1, BC032995.1 and AC116505.13.
            
            On Sep 9, 2005 this sequence version replaced XM_355574.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.155418.1,
                                           SRR1660815.81276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                BY740128.1         1-58
            59-1136             BC032995.1         1-1078
            1137-3193           AC116505.13        128346-130402
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 8.16 cM"
     gene            1..3193
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /note="G protein subunit alpha i1"
                     /db_xref="GeneID:14677"
                     /db_xref="MGI:MGI:95771"
     exon            1..362
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..1309
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /note="adenylate cyclase-inhibiting G alpha protein;
                     guanine nucleotide binding protein, alpha inhibiting 1;
                     heterotrimeric guanine nucleotide-binding protein 1b;
                     guanine nucleotide binding protein (G protein), alpha
                     inhibiting 1"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein G(i) subunit
                     alpha-1"
                     /protein_id="NP_034435.1"
                     /db_xref="CCDS:CCDS39018.1"
                     /db_xref="GeneID:14677"
                     /db_xref="MGI:MGI:95771"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGES
                     GKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARAD
                     DARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLD
                     RIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV
                     TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEE
                     KIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQF
                     VFDAVTDVIIKNNLKDCGLF"
     exon            363..405
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            406..547
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            548..705
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            706..834
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            835..964
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1118
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..3193
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3168..3173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /note="hexamer: AATAAA"
     polyA_site      3193
                     /gene="Gnai1"
                     /gene_synonym="Gialpha1; Gnai-1; Hg1b"
                     /note="major polyA site"
ORIGIN      
        1 acgggcagct gcggtggcgg cggcggcggc ggcggcggca gagcctagcg gtcgcaggga
       61 ttcgcgggcg cggatcgcgg agtgtcgccg gcagggacgg ccgcccgagg cggcagcgtg
      121 cggactagca gacctcgcct ccagcttgcg ggagctgagg acgtagcggg gccgcctccg
      181 ggccagcctc tcctcgctct gccagccggc cccggcgccg agggcgcggc gacgctcggc
      241 caccatgggc tgcacattga gcgctgagga caaggcggcc gtggagcgca gcaagatgat
      301 cgaccgcaac ctccgggagg acggcgagaa ggcggcgcgg gaggtcaagc tgctgctgct
      361 gggtgctggg gaatctggaa agagtaccat tgtgaagcag atgaagatta tccacgaagc
      421 cggctactcg gaagaggagt gtaagcagta caaggcagtg gtctacagca acactatcca
      481 gtccatcatt gccatcatta gagccatggg gaggttgaaa atcgacttcg gagactctgc
      541 tcgggcggat gatgctcgcc aacttttcgt gcttgctggg gctgcagaag aaggctttat
      601 gactgcagag ctcgccggtg tcataaagag actgtggaaa gacagtggtg tgcaagcctg
      661 cttcaacaga tcccgggagt accagctgaa cgattcggca gcgtactatc tgaatgactt
      721 ggacagaata gcacagccaa attacatccc aactcagcag gatgtcctca gaaccagagt
      781 gaagaccaca gggattgtgg aaacccactt taccttcaaa gatcttcatt ttaaaatgtt
      841 tgacgtggga ggtcagaggt cagagcggaa gaagtggatc cactgctttg aaggggtgac
      901 cgccatcatc ttctgtgtgg ccctgagtga ctatgacctg gttcttgctg aagatgaaga
      961 aatgaaccgt atgcacgaga gcatgaagct gttcgatagc atctgtaaca acaagtggtt
     1021 tacagacacg tccatcatcc ttttcctcaa caagaaggac ctcttcgaag aaaaaataaa
     1081 aaagagcccc ctcacgatat gctacccaga atatgcaggc tcaaacacat atgaagaagc
     1141 ggccgcgtat attcagtgtc agtttgaaga cctcaataaa aggaaggaca caaaggaaat
     1201 ttacacccac ttcacgtgcg ccacagatac gaagaacgtg cagttcgtgt tcgatgctgt
     1261 aacagacgtc atcataaaga ataacctaaa agactgtggt ctcttctaag ttctgcagtg
     1321 ggtagtaaaa aagcattttc caacagatga gtacttatat atggatctct ctagactaga
     1381 gtgtcccagc aactcagaat gtagtgtaca gcaaatgcat cctggacctg accagagttc
     1441 tgaccaaagt tgttctgtat tgtatcttta tctgaacgta atagaaggac ctgtcttgaa
     1501 ggagaaagat ggccctgggg tgtgggaggg aggagtgatg ttgaagctgg tctctagtgt
     1561 actgatgatt tctgtgtagg tgtaaatatg cagatgtaag atgtactcat aacttttgat
     1621 ttcccacata tgtttaggtt gtaagagtgg caactttgat tctagtgtga tggctttgga
     1681 aaaaaacata aatattaagt accttgtatg gaatgacaga ttggttcttt gtctggcagt
     1741 tttaatcagc tttatttatg ttcctgcccc taaattttag gtaccataat taatattagg
     1801 aaatgtcact gcccttaccc tgaatctatg tttacttgta ttcgtaaaat gttatttgta
     1861 caaacattgc acagactatt ttaataacat gatttgtcct ctaacgttta cgtgtttcct
     1921 gaggatgttc ttacagaggt tggatgcacc tgcctttgga tcaattattt aagcattgag
     1981 tgcattagaa tttatttaca gagtgcatga tgttctgact ctagtttaga tttatttaag
     2041 aaaccaaatc tccagatttt ttcaggacac aggatcttag acttaaaagt ccttcacttt
     2101 taaatccaca actttgtttc aatttggtct tagtatctgg atagaatttc tgcattgcct
     2161 tggagtgatg ctaaattaat tttatacact taaatgacca ctgttttatt tataatgaag
     2221 tttagatgag tgatgaaagc atttttgtgg gtaaaaactt ttagtattat gtgaattttg
     2281 agacattgat ttatgtattt ttttgacaaa gcccttgtat ttaagcatat atgatacttc
     2341 cttgtctgga agaaaatcag aaaatatgat attgctgata ttatacgagc ttttgattca
     2401 tatggaacta gtttcaagtt catcacttta tgtcaaaaaa tgacttcttt tgcagtaggt
     2461 attcaaattc cttccacttg cttgaattta tattctttta aagctattgt gtgatttctg
     2521 aaattaagtg gacaattata agtagaaatt gagtaatagt atttaacaag ataagtgagt
     2581 gtatgtgggc ttaaatagat gttacaatac agctacatac tgaactttgt tcgtgccatt
     2641 gaaacaaaat catttacaat gtgtgtgcgc cctggtttca gcaagctgac tcgccttccc
     2701 agctccttct ccttttgaac tcatcctgca acattaactc tgtccacgag ctttgctggt
     2761 gcatagttgc tcacttgagt taggtgttgc cagttcccca gagtctccct gttgaagttt
     2821 gtgtatatgt aagcgtgtgg agaactgtaa actacattca cacctgttta ctttgagcaa
     2881 atttgtttcc ttctttgtaa tgtaaagaaa ttcagcgtta ccaaattcct tcagtgtgag
     2941 tttagattat tttatgtgcc tttcatgaaa gatccatttc cattcattta ctaatggaag
     3001 acctgtcaga cccacattgc gaagctatgt atgaaattca agtatactct ggataaattt
     3061 gaatggtgaa aagtagtgtt taaaagccac aaagaagcac atattggtga ccatcattgg
     3121 tgaatccctg aactctagtc tgtgtaactg tgctactaat acatcccaat aaatctaaat
     3181 ttttaaaatt tta
//
