LOCUS       NM_001308449            1007 bp    mRNA    linear   ROD 18-APR-2023
DEFINITION  Mus musculus predicted gene, 27021 (Gm27021), mRNA.
ACCESSION   NM_001308449
VERSION     NM_001308449.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Bonaldo MF, Lennon G and Soares MB.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC103360.9, BQ746342.1, AI844196.1 and AEKQ02009813.1.
            
            Summary: This locus represents naturally occurring readthrough
            transcription between the neighboring ER membrane protein complex
            subunit 8 and RIKEN cDNA 1190005I06 genes on chromosome 8. The
            readthrough transcript encodes a fusion protein that shares
            sequence identity with each individual gene product. [provided by
            RefSeq, May 2015].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BQ746342.1, CX224265.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 18117, 68918
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AC103360.9         32572-32694
            124-776             BQ746342.1         1-653
            777-1005            AI844196.1         17-245              c
            1006-1007           AEKQ02009813.1     17098-17099         c
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8"
     gene            1..1007
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /note="predicted gene, 27021"
                     /db_xref="GeneID:105734727"
                     /db_xref="MGI:MGI:5504136"
     exon            1..386
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..635
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /note="Emc8-1190005I06Rik readthrough"
                     /codon_start=1
                     /product="uncharacterized protein LOC105734727"
                     /protein_id="NP_001295378.1"
                     /db_xref="GeneID:105734727"
                     /db_xref="MGI:MGI:5504136"
                     /translation="MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPG
                     AGSHTLFVDCIPLFHGTLALTPMLEVALTLARRGDAMGLKPSCLKGFKMCVSSSNNNH
                     DEAPVLNDKHLSVPNIIITPPTPTGMGLSRDSNKQVWMDELGSYQDDGELEPEA"
     exon            387..432
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /inference="alignment:Splign:2.1.0"
     exon            433..576
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /inference="alignment:Splign:2.1.0"
     exon            577..1007
                     /gene="Gm27021"
                     /gene_synonym="Emc8-1190005i06rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggcggggc ggtaactgtc gcgaaaggcc gagcgggtag cagctgcttt ccggcagacg
       61 ttttccggcc gaccggctga ggcgggaggc cgcggccgga ggtgcgagag cgtcacggcg
      121 tgcagcggag ggtcgtggcg gaggaccgcg gtgcgtgggt cgcgatgccc ggcgtgaagc
      181 tgactacgca ggcctactgc aagatggtgc tccacggcgc caagtacccg cactgcgccg
      241 tcaacgggct cctggtggcc gagaggcaga ggccgcgcaa ggagcatcct cccggagcgg
      301 gcagccacac gctcttcgtg gactgcatcc cgctcttcca cggcacgctg gctctgacgc
      361 ccatgctgga ggtggcgctc accctggccc gcagaggcga cgccatgggg ctgaagccct
      421 cctgtctgaa aggctttaag atgtgtgtca gcagcagcaa caacaaccac gacgaggccc
      481 ccgtcctgaa tgacaagcac ctgagcgtgc ccaacattat catcacgccc ccaaccccga
      541 cgggcatggg gctttcccgg gattctaaca agcaagtctg gatggatgag ctggggtctt
      601 atcaagacga tggagagctg gaacctgaag cctgaagcgg cgagacctgt gtggcagcaa
      661 cctgccccgc tgccccgggt gttgggggcg tggctacctg agcagctgga tggggaacag
      721 cgaattgggt cctggaccaa gacatggatg gccagaagag tacagcaggt ggctggcctc
      781 aacggagcag acatccgtgg cctctgagtc cgcaccaacc cagaccccgg cttggtgaag
      841 aggaggccac cctgccctac ggtcaccgtg accatggacc tcggccctcg aggccccaga
      901 gcacaaggac ttctcgccag agcagggcct ctgtttttta caagttgttt tacaggtatg
      961 ggacctacat cctgagcgag atttataaat aaagctcctt tgtgact
//
