LOCUS       NM_001364933             864 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus predicted gene 20517 (Gm20517), transcript variant 1,
            mRNA.
ACCESSION   NM_001364933
VERSION     NM_001364933.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Gaudet,P., Livstone,M.S., Lewis,S.E. and Thomas,P.D.
  TITLE     Phylogenetic-based propagation of functional annotations within the
            Gene Ontology consortium
  JOURNAL   Brief Bioinform 12 (5), 449-462 (2011)
   PUBMED   21873635
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139214.12 and AK031504.1.
            
            Summary: This locus represents naturally-occurring readthrough
            transcription between the neighboring mediator complex subunit 20
            (Med20) and ubiquitin specific peptidase 49 (Usp49) genes.
            Readthrough transcripts may be candidates for nonsense mediated
            decay (NMD) or encode proteins with similarity to the Med20 gene.
            [provided by RefSeq, Jul 2018].
            
            Transcript Variant: This variant (1) includes sequence from both
            Med20 and the 5' UTR of Usp49 and encodes a protein with similarity
            to mediator complex subunit 20.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031504.1, CK331495.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 56771, 224836
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC139214.12        201961-201973
            14-250              AK031504.1         2-238
            251-251             AC139214.12        203481-203481
            252-864             AK031504.1         239-851
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 23.66 cM"
     gene            1..864
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /note="predicted gene 20517"
                     /db_xref="GeneID:112694759"
                     /db_xref="MGI:MGI:5141982"
     exon            1..108
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..547
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /note="Med20-Usp49 readthrough; Mediator of RNA polymerase
                     II transcription subunit 20; Mediator complex subunit 20;
                     TRF-proximal protein homolog"
                     /codon_start=1
                     /product="uncharacterized protein LOC112694759"
                     /protein_id="NP_001351862.1"
                     /db_xref="GeneID:112694759"
                     /db_xref="MGI:MGI:5141982"
                     /translation="MGVTCVSQMPVAEGKSLQQTVELLTKKLEMLGAEKQGTFCVDCE
                     TYHTAASTLGSQGQAGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFF
                     QSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVTEKRKPSQ"
     exon            109..263
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /inference="alignment:Splign:2.1.0"
     exon            264..517
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /inference="alignment:Splign:2.1.0"
     exon            518..864
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      841..846
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /note="hexamer: AATAAA"
     polyA_site      864
                     /gene="Gm20517"
                     /gene_synonym="Med20; Trfp"
                     /note="major polyA site"
ORIGIN      
        1 gactgcttcc tgtgtttagg gggccgccgt tcgcgtgaag gtctggagtc tctgagttcg
       61 agggtttgga ggcgccacct tttgatcctt cgctatggga gtgacgtgtg tgtcccagat
      121 gcctgtggct gagggcaaga gtctccagca gacagtggag ctcctcacca agaaactgga
      181 gatgcttgga gcagagaaac aagggacatt ttgtgtggac tgtgagactt accacacagc
      241 tgcctctacc ctgggcagcc aaggccaggc tgggaagctg atgtacgtga tgcataactc
      301 tgagtaccca ctgagctgct ttgccctctt tgagaatggc ccttgcctca ttgctgacac
      361 caacttcgat gtgcttatgg tgaagctcaa gggcttcttc cagagtgcca aggccagcaa
      421 gattgagacc cggggcactc gctaccagta ttgtgacttc ctggtgaagg tgggcacggt
      481 cacaatgggg cccagtgccc gtggcatctc tgtggaggta acagagaaga ggaaacccag
      541 ccagtgataa tgtgagaata tggaaggcga tgggagcagc ttgtttaatg ggtaaagaag
      601 aaccttactg tgggaaacct tattaatcct gtcaggggag ggagtagcca tgaccagtgt
      661 ctcagtgatg ttctgaactt tcggtaaagt cttgggtagc ttgaagttta ttgatggaga
      721 gactgtgctt gccatcgcct gtcaagaatg attagccacc ccctattctc tgaacactgc
      781 cagctgctgt gataaaggct caccatggag gtcttgttca ctttagcact gtggtattca
      841 aataaaattt caaaagtcca taaa
//
