LOCUS       NM_001045542            1356 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus predicted gene 12253 (Gm12253), mRNA.
ACCESSION   NM_001045542 XM_889407 XM_905245 XM_986428
VERSION     NM_001045542.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
COMMENT     INFERRED REFSEQ: This record is predicted by genome sequence
            analysis and is not yet supported by experimental evidence. The
            reference sequence was derived from AL663107.13.
            
            On or before Sep 2, 2006 this sequence version replaced
            XM_889407.2, XM_905245.2, XM_986428.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN00849383 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on single protein-coding
                                         transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                AL663107.13        75600-75694
            96-185              AL663107.13        77540-77629
            186-296             AL663107.13        77831-77941
            297-440             AL663107.13        78398-78541
            441-569             AL663107.13        79707-79835
            570-690             AL663107.13        82927-83047
            691-1356            AL663107.13        83998-84663
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 36.19 cM"
     gene            1..1356
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /note="predicted gene 12253"
                     /db_xref="GeneID:624860"
                     /db_xref="MGI:MGI:3651568"
     exon            1..95
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     exon            96..185
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..790
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /codon_start=1
                     /product="Protein RoBo-1 precursor"
                     /protein_id="NP_001039007.1"
                     /db_xref="CCDS:CCDS36164.1"
                     /db_xref="GeneID:624860"
                     /db_xref="MGI:MGI:3651568"
                     /translation="MEVTMSWFLVLKSLLAVCIISHLSVSSVESYACIQEEPPFNKEC
                     YPNSSTCESSKGCFSLWLELGAHQTYVQKGCSEDSCTGLEFSVTVERHLALSYDHRCC
                     YTEHCSKEPIKVPALSSEPNGVECPACFSECGTCRPVLLKCTGEETMCIKATARDIIK
                     LQAMGCATKTACNLKNVTITDNLKFDTSCVNGSPLLSSRLLLQHHACLPAATLPAMTV
                     MELSSGTVSPQ"
     exon            186..296
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     exon            297..440
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     exon            441..569
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     exon            570..690
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     exon            691..1356
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /inference="alignment:Splign:2.1.0"
     regulatory      786..791
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /note="hexamer: AATAAA"
     polyA_site      807
                     /gene="Gm12253"
                     /gene_synonym="Gm248"
                     /note="major polyA site"
ORIGIN      
        1 tatccctgac tggcctgctg tgtagacttg agaccactgg cagtaggtga cagcccagct
       61 gaggttaatc gaagtctcat cctagggcct ctgcttgttc atggaggtca ccatgtcctg
      121 gttcttggtc ctgaagagcc tccttgccgt ctgcatcatc tctcacttat ctgtcagctc
      181 tgtggagagt tatgcttgta tacaagaaga accacccttt aataaagaat gctatcctaa
      241 ttcaagcacg tgtgaatcct ctaaaggttg tttctcccta tggctggaat tgggagcaca
      301 tcaaacatat gtgcagaaag gctgttctga agacagttgt actggactag aattctcggt
      361 aacagtggag aggcatttgg cattgagcta tgaccatcgc tgctgctaca ctgaacactg
      421 tagcaaagag cccattaagg tgcctgcatt atcttcagaa cccaatggcg ttgaatgtcc
      481 tgcctgcttc agtgaatgtg gcacatgtag acctgtttta ctaaagtgca cgggggagga
      541 gacaatgtgc attaaggcta ctgccagaga catcatcaag ctacaagcca tgggctgtgc
      601 gaccaagact gcctgcaacc tgaagaacgt gaccatcacg gataatctta aatttgatac
      661 ctcctgtgtc aatgggagcc ctctgctcag ctctcggcta ctgctccaac accatgcctg
      721 cctgcctgct gctacgctcc ccgccatgac ggttatggag ttatcctctg gaactgtgag
      781 cccccaataa actctttctt ttagaaattt ccttggtcat ggggtcttca cagtaataga
      841 aaagtaacta agacgtcatc cttggaaatg tagtagcatc aaaatctccc ttttaataag
      901 aatagcaagg atcaggacct gctaaagtgg tctcatttta atttgatcat ttctgtaaag
      961 atcttgcctt ggactggaga gatggcttag aggttaggag cactgactgc tttttcagag
     1021 atcctgagtt caattcccag caaccacatg gtggctcaca accatctgta ctgggatcag
     1081 atgccctctt ctggcatgtc tgaagacagc cacagtgtac tcacatacat acaattaaaa
     1141 aacaaacaaa caaacaaaca aacaatcttg tcaacttaac acaaaccagg gtcacctggg
     1201 aagagagaac ctcaacagag aaaaatgcct ccatcagact ggcttgtaga gaaatttgtg
     1261 gagcattttt ttgattgatg tttgatgtgg ggcccagccc actgtgggca gtaccatccc
     1321 cagccaagtg gttctgggag gtgtaagaaa ggagga
//
