LOCUS       NM_008131               2790 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus glutamate-ammonia ligase (Glul), mRNA.
ACCESSION   NM_008131
VERSION     NM_008131.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Kawase,K., Nakamura,Y., Wolbeck,L., Takemura,S., Zaitsu,K.,
            Ando,T., Jinnou,H., Sawada,M., Nakajima,C., Rydbirk,R., Gokenya,S.,
            Ito,A., Fujiyama,H., Saito,A., Iguchi,A., Kratimenos,P.,
            Ishibashi,N., Gallo,V., Iwata,O., Saitoh,S., Khodosevich,K. and
            Sawamoto,K.
  TITLE     Significance of birth in the maintenance of quiescent neural stem
            cells
  JOURNAL   Sci Adv 11 (4), eadn6377 (2025)
   PUBMED   39841848
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Wutikeli,H., Yu,Y., Zhang,T., Cao,J., Nawy,S. and Shen,Y.
  TITLE     Role of Elavl-like RNA-binding protein in retinal development and
            signal transduction
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1871 (1), 167518 (2025)
   PUBMED   39307290
REFERENCE   3  (bases 1 to 2790)
  AUTHORS   Lyu,J., Gu,Z., Zhang,Y., Vu,H.S., Lechauve,C., Cai,F., Cao,H.,
            Keith,J., Brancaleoni,V., Granata,F., Motta,I., Cappellini,M.D.,
            Huang,L.J., DeBerardinis,R.J., Weiss,M.J., Ni,M. and Xu,J.
  TITLE     A glutamine metabolic switch supports erythropoiesis
  JOURNAL   Science 386 (6723), eadh9215 (2024)
   PUBMED   39541460
REFERENCE   4  (bases 1 to 2790)
  AUTHORS   Ratsika,A., Codagnone,M.G., Bastiaanssen,T.F.S., Hoffmann
            Sarda,F.A., Lynch,C.M.K., Ventura-Silva,A.P., Rosell-Cardona,C.,
            Caputi,V., Stanton,C., Fulling,C., Clarke,G. and Cryan,J.F.
  TITLE     Maternal high-fat diet-induced microbiota changes are associated
            with alterations in embryonic brain metabolites and adolescent
            behaviour
  JOURNAL   Brain Behav Immun 121, 317-330 (2024)
   PUBMED   39032541
REFERENCE   5  (bases 1 to 2790)
  AUTHORS   Madsen,S., Delgado,A.C., Cadilhac,C., Maillard,V., Battiston,F.,
            Igelbuscher,C.M., De Neck,S., Magrinelli,E., Jabaudon,D.,
            Telley,L., Doetsch,F. and Knobloch,M.
  TITLE     A fluorescent perilipin 2 knock-in mouse model reveals a high
            abundance of lipid droplets in the developing and adult brain
  JOURNAL   Nat Commun 15 (1), 5489 (2024)
   PUBMED   38942786
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2790)
  AUTHORS   Miyake,T. and Kitamura,T.
  TITLE     Glutamine synthetase immunoreactivity in two types of mouse brain
            glial cells
  JOURNAL   Brain Res 586 (1), 53-60 (1992)
   PUBMED   1380881
REFERENCE   7  (bases 1 to 2790)
  AUTHORS   Wichmann,H., Jockusch,H., Guenet,J.L., Gallwitz,D. and Kaupmann,K.
  TITLE     The mouse homolog to the ras-related yeast gene YPT1 maps on
            chromosome 11 close to the wobbler (wr) locus
  JOURNAL   Mamm Genome 3 (8), 467-468 (1992)
   PUBMED   1643310
REFERENCE   8  (bases 1 to 2790)
  AUTHORS   Cornall,R.J., Friedman,J.M. and Todd,J.A.
  TITLE     Mouse microsatellites from a flow-sorted 4:6 Robertsonian
            chromosome
  JOURNAL   Mamm Genome 3 (11), 620-624 (1992)
   PUBMED   1450512
REFERENCE   9  (bases 1 to 2790)
  AUTHORS   Bhandari,B., Roesler,W.J., DeLisio,K.D., Klemm,D.J., Ross,N.S. and
            Miller,R.E.
  TITLE     A functional promoter flanks an intronless glutamine synthetase
            gene
  JOURNAL   J Biol Chem 266 (12), 7784-7792 (1991)
   PUBMED   1673462
REFERENCE   10 (bases 1 to 2790)
  AUTHORS   Love,J.M., Knight,A.M., McAleer,M.A. and Todd,J.A.
  TITLE     Towards construction of a high resolution map of the mouse genome
            using PCR-analysed microsatellites
  JOURNAL   Nucleic Acids Res 18 (14), 4123-4130 (1990)
   PUBMED   2377456
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099702.12.
            
            On Jul 6, 2022 this sequence version replaced NM_008131.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK168493.1, AK162685.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC099702.12        128169-128282       c
            115-293             AC099702.12        125116-125294       c
            294-455             AC099702.12        123530-123691       c
            456-602             AC099702.12        121733-121879       c
            603-730             AC099702.12        121081-121208       c
            731-930             AC099702.12        120743-120942       c
            931-2790            AC099702.12        118505-120364       c
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 65.43 cM"
     gene            1..2790
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /note="glutamate-ammonia ligase"
                     /db_xref="GeneID:14645"
                     /db_xref="MGI:MGI:95739"
     exon            1..114
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     exon            115..293
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..1249
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /EC_number="6.3.1.2"
                     /EC_number="2.3.1.225"
                     /note="glutamate-ammonia ligase (glutamine synthase);
                     glutamate decarboxylase; palmitoyltransferase GLUL;
                     glutamate-ammonia ligase (glutamine synthetase)"
                     /codon_start=1
                     /product="glutamine synthetase"
                     /protein_id="NP_032157.2"
                     /db_xref="CCDS:CCDS15381.1"
                     /db_xref="GeneID:14645"
                     /db_xref="MGI:MGI:95739"
                     /translation="MATSASSHLNKGIKQMYMSLPQGEKVQAMYIWVDGTGEGLRCKT
                     RTLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLHPVAMFRDPFRKDPNKLVLCEVF
                     KYNRKPAETNLRHICKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGP
                     YYCGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHL
                     WIARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKCIEEAIDK
                     LSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRGASIRIPRTVGQEKK
                     GYFEDRRPSANCDPYAVTEAIVRTCLLNETGDEPFQYKN"
     exon            294..455
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     exon            456..602
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     exon            603..730
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     exon            731..930
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     exon            931..2790
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2706..2711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /note="hexamer: TATAAA"
     polyA_site      2726
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
                     /note="major polyA site"
     polyA_site      2790
                     /gene="Glul"
                     /gene_synonym="Glns; GS"
ORIGIN      
        1 agagcggaga atgggagtag agcagagtgt ctgaacagca cgctcaccca tctcctctcc
       61 gcctcgctct cctgacctgt tcacccatcc atcatccggc cggccaccgc tctgaacacc
      121 ttccaccatg gccacctcag caagttccca cttgaacaaa ggcatcaagc aaatgtacat
      181 gtccctgccc cagggtgaga aagtccaagc catgtatatc tgggttgatg gtaccggaga
      241 aggactgcgc tgcaagaccc gtaccctgga ctgtgagccc aagtgtgtgg aagagttacc
      301 tgagtggaac tttgatggct ctagtacctt tcagtctgaa ggctccaaca gcgacatgta
      361 cctccatcct gttgccatgt ttcgagaccc cttccgcaaa gaccccaaca agctggtgct
      421 atgtgaagtt ttcaagtata accggaaacc tgcagagacc aacttgaggc acatctgtaa
      481 acggataatg gacatggtga gcaaccagca cccctggttt ggaatggagc aggaatatac
      541 tcttatggga acagacggcc acccatttgg ttggccttcc aatggcttcc ctggacccca
      601 aggcccgtat tactgcggtg tgggagcaga caaggcctac ggcagggaca tcgtggaggc
      661 tcactaccgg gcctgcttgt atgctggagt caagattacg gggacaaatg cggaggttat
      721 gcctgcccag tgggaattcc agataggacc ctgtgagggg atccgaatgg gagatcatct
      781 ttggatagcc cgttttatct tgcatcgggt gtgcgaagac tttggggtga tagcaacctt
      841 tgaccccaag cccattccag ggaactggaa tggtgcaggc tgccatacca acttcagcac
      901 caaggccatg cgggaggaga atggtctgaa gtgcattgag gaggccattg acaaactgag
      961 caagaggcac cagtaccaca ttcgcgccta cgatcccaag gggggcctgg acaacgcccg
     1021 gcgtctgact ggattccacg aaacctccaa catcaacgac ttttctgccg gtgttgccaa
     1081 ccgcggtgcc agtatccgca ttccccggac tgtcggccag gagaagaagg gctactttga
     1141 agaccgtcgg ccttctgcca attgtgaccc ctatgcggtg acagaagcca tcgtccgcac
     1201 gtgtctcctc aacgaaacag gcgacgaacc cttccaatac aagaactaag tggactagac
     1261 ttccagtgat ccctctccca gctcttccct ttcccagttg tccccactgt aactcaaaag
     1321 gatggaatac caaggtcttt ttattcctcg tgcccagtta atcttgcttt tgttggtcag
     1381 aatagagggg tcaggttctt aatctctaca caccaaccct ttctttccta tctagctttc
     1441 tagtagggag cgggaggggg gaggggaagg gtaacccact gcttcatctc atcgggtatg
     1501 catgtccggt aggcatagct gtcacaaagc gggtgtactt atggtgaaag aggacatttt
     1561 ttttttcttc aggatagttg aaagggcagg cccaacggct gagattgaca tttccactgt
     1621 tggtagagag ctgttatttc taaaggggaa accagctttc tgttccaaat ggaagttagg
     1681 tgaggagttg aaggttggtt tcttgcgctg tgcttccttg gcttggggga gggggcatcc
     1741 gtccccctct gtgtgaacac agctcaccgc gtcacctgat ggatggccct actgtgaagg
     1801 aagaaaaaag ttggcatttc ttggtcctcc gttcataaca caaagcagag tagtattttt
     1861 atatttaaat gttaaaaaca aaaaagttat atatatatgg atgtgtggat gtatgtcttt
     1921 ctaattgaga gaaccatcct attcactggg tgccaagttt gagtgatgag gggcttggct
     1981 tagaagtgag gctcccttga ggtaggggtg aggatgcagt accgggaaag ttggttatct
     2041 tggggtctca gcttcattac tgcttagggt ttccctgccc actctgcagg agcagatgtt
     2101 ggacaggtag ccagtgggat gccactgctt gccaccacct gtccccaggc ttaggtttag
     2161 gggatgcgta tacttactcc acacacgagt tagaagtatg agttggctgg tcaacttgaa
     2221 cactgttact gatgggtggg tgggtggggg tttactgggg ttattttttt ggtgggatta
     2281 gcatgtcact aaagcgggcc ttttgatata ttaagttttt taaaagcaaa acaagtttag
     2341 attttaatca gatttgtagg gtttctaact ttacagaatt gcctgtttgt ttcaatgtct
     2401 ccctccactt ggctcttagg ggaattaagg acaggcctag agttaaaaca cttgtctcct
     2461 agtgtcacct ctgccagcag actgttactt tccttctgaa aaagccaata gtcttttttt
     2521 ttttctttta tagtaaacac acccccacct ccatcccagc ctgttgccct tcagttttct
     2581 ggttgtttgt gtcggcagcg ggccaactgt ggtttctctc ttgccatgat gacttctaat
     2641 tgccatgtat agtatgttcg gttagataac tcactgtaaa cagactgtaa ctgccaggca
     2701 gcgcttataa atcaacctaa catttataag atttcctctg acttgtttct ttgtggttcc
     2761 caaaaaaaaa aaaaaaacct caaaaattaa
//
