LOCUS       NM_001033264            2460 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus glutaminase 2 (liver, mitochondrial) (Gls2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001033264 XM_125928
VERSION     NM_001033264.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Hu,J., Zhang,Z., Hu,H., Yang,K., Zhu,Z., Yang,Q. and Liang,W.
  TITLE     LRH-1 activation alleviates diabetes-induced podocyte injury by
            promoting GLS2-mediated glutaminolysis
  JOURNAL   Cell Prolif 56 (11), e13479 (2023)
   PUBMED   37057309
  REMARK    GeneRIF: LRH-1 activation alleviates diabetes-induced podocyte
            injury by promoting GLS2-mediated glutaminolysis.
REFERENCE   3  (bases 1 to 2460)
  AUTHORS   Rong,R., Wei,Y., Li,L., Wang,T., Zhu,H., Xiao,G. and Wang,Y.
  TITLE     Image-based quantification of histological features as a function
            of spatial location using the Tissue Positioning System
  JOURNAL   EBioMedicine 94, 104698 (2023)
   PUBMED   37453365
REFERENCE   4  (bases 1 to 2460)
  AUTHORS   Patel,T., Hammelman,J., Aziz,S., Jang,S., Closser,M.,
            Michaels,T.L., Blum,J.A., Gifford,D.K. and Wichterle,H.
  TITLE     Transcriptional dynamics of murine motor neuron maturation in vivo
            and in vitro
  JOURNAL   Nat Commun 13 (1), 5427 (2022)
   PUBMED   36109497
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2460)
  AUTHORS   Martinez-Ramirez,A.S., Borders,T.L., Paul,L., Schipma,M., Wang,X.,
            Korobova,F., Wright,C.V. and Sosa-Pineda,B.
  TITLE     Specific Temporal Requirement of Prox1 Activity During Pancreatic
            Acinar Cell Development
  JOURNAL   Gastro Hep Adv 1 (5), 807-823 (2022)
   PUBMED   37829188
REFERENCE   6  (bases 1 to 2460)
  AUTHORS   Le,A., Lane,A.N., Hamaker,M., Bose,S., Gouw,A., Barbi,J.,
            Tsukamoto,T., Rojas,C.J., Slusher,B.S., Zhang,H., Zimmerman,L.J.,
            Liebler,D.C., Slebos,R.J., Lorkiewicz,P.K., Higashi,R.M., Fan,T.W.
            and Dang,C.V.
  TITLE     Glucose-independent glutamine metabolism via TCA cycling for
            proliferation and survival in B cells
  JOURNAL   Cell Metab 15 (1), 110-121 (2012)
   PUBMED   22225880
REFERENCE   7  (bases 1 to 2460)
  AUTHORS   Suzuki,S., Tanaka,T., Poyurovsky,M.V., Nagano,H., Mayama,T.,
            Ohkubo,S., Lokshin,M., Hosokawa,H., Nakayama,T., Suzuki,Y.,
            Sugano,S., Sato,E., Nagao,T., Yokote,K., Tatsuno,I. and Prives,C.
  TITLE     Phosphate-activated glutaminase (GLS2), a p53-inducible regulator
            of glutamine metabolism and reactive oxygen species
  JOURNAL   Proc Natl Acad Sci U S A 107 (16), 7461-7466 (2010)
   PUBMED   20351271
  REMARK    GeneRIF: Phosphate-activated glutaminase (GLS2) is a p53-inducible
            regulator of glutamine metabolism and reactive oxygen species
REFERENCE   8  (bases 1 to 2460)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   9  (bases 1 to 2460)
  AUTHORS   Willnow,T., Antignac,C., Brandli,A., Christensen,E., Cox,R.,
            Davidson,D., Davies,J., Devuyst,O., Eichele,G., Hastie,N.,
            Verroust,P., Schedl,A. and Meij,I.
  TITLE     The European renal genome project: an integrated approach towards
            understanding the genetics of kidney development and disease
  JOURNAL   Organogenesis 2 (2), 42-47 (2005)
   PUBMED   19521566
REFERENCE   10 (bases 1 to 2460)
  AUTHORS   Perez-Gomez,C., Mates,J.M., Gomez-Fabre,P.M., del
            Castillo-Olivares,A., Alonso,F.J. and Marquez,J.
  TITLE     Genomic organization and transcriptional analysis of the human
            l-glutaminase gene
  JOURNAL   Biochem J 370 (Pt 3), 771-784 (2003)
   PUBMED   12444921
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135859.4 and AK220231.1.
            
            On Jul 29, 2009 this sequence version replaced NM_001033264.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC141115.1, AK220231.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC135859.4         112794-112841       c
            49-2393             AK220231.1         1-2345
            2394-2460           AC135859.4         97472-97538         c
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 76.44 cM"
     gene            1..2460
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /note="glutaminase 2 (liver, mitochondrial)"
                     /db_xref="GeneID:216456"
                     /db_xref="MGI:MGI:2143539"
     exon            1..273
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..1900
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /EC_number="3.5.1.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; liver mitochondrial glutaminase; L-glutaminase;
                     L-glutamine amidohydrolase"
                     /codon_start=1
                     /product="glutaminase liver isoform, mitochondrial isoform
                     1 precursor"
                     /protein_id="NP_001028436.2"
                     /db_xref="CCDS:CCDS24263.1"
                     /db_xref="GeneID:216456"
                     /db_xref="MGI:MGI:2143539"
                     /translation="MRSMRALQNALSRAGSHGRRGGWGHPSRGPLLGRGVRYYLGEAA
                     AQGRGTPHSHQPQHSDHDASHSGMLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQ
                     TSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEF
                     TGHVDRIFEDAKEPTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQ
                     SCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSS
                     LIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCF
                     PKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSL
                     MHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSQRGINF
                     CQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALS
                     AMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHL
                     EVVKLLQDYHDSYLLSETQAEAAAETLSKENLESMV"
     exon            274..373
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            374..495
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            496..625
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            626..705
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            706..869
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            870..928
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            929..961
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1020
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1087
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1138
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1315
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1447
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1540
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1602
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1679
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1743
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..2460
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2375..2380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /note="hexamer: AATAAA"
     regulatory      2395..2400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /note="hexamer: ATTATA"
     polyA_site      2395
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
                     /note="major polyA site"
     polyA_site      2424
                     /gene="Gls2"
                     /gene_synonym="A330074B06Rik; GA; GLS; Lga"
ORIGIN      
        1 ctagtctcgc ccgccactct tccgcccctc cctcctcgcc gccgctcgca cgtctggagc
       61 gcccgggccg agcgggtggc gcgccggggg catgcgctcc atgagggctc tgcagaacgc
      121 gctgagccgc gcgggcagcc acggtcggcg gggaggctgg ggtcatccga gccggggccc
      181 gcttctgggc aggggcgtcc ggtactacct cggtgaggcc gcagcgcagg gcagggggac
      241 gccgcacagc caccagccgc agcactcgga tcatgacgcc tcacacagcg gcatgctgcc
      301 tcgacttggt gacctgcttt tctacactat tgcagaggga caggagcgta tccctatcca
      361 caagttcacc acggctctga aggccactgg actgcagacg tcagacccac ggctccagga
      421 ctgcatgagc aagatgcagc gcatggtcca agagtccagc agcggtggcc tcttggaccg
      481 agagctcttc caaaagtgtg tgagcagcaa cattgtgctc ctgactcagg cattccgaaa
      541 gaagtttgtc attcctgact ttgaggagtt cacgggccat gtggatcgca tatttgagga
      601 tgccaaagag cccactggag gcaaagtggc agcctacatc ccgcacctgg ccaaatcaaa
      661 cccagacctt tggggcgtct ccctgtgcac tgtggatggg cagcggcact ctgtgggcca
      721 cacgaagatc cccttctgcc tgcagtcctg tgtcaagccc ctcacttacg ccatctccgt
      781 gagcacctta ggcactgact acgtgcacaa gtttgtgggc aaggaaccca gtggtctgcg
      841 ctataacaaa ctctccctca atgaggaagg aattccccat aaccccatgg tcaatgctgg
      901 tgccattgtg gtcagctccc tgatcaagat ggactgtaac aaagcagaga agttcgattt
      961 tgtgttacag tatctgaaca agatggctgg gaacgaattc atggggttca gcaatgccac
     1021 attccagtca gagaaggaga ctggggatcg gaattacgcc atcggctatt acctcaagga
     1081 gaagaagtgc ttccctaagg gtgtggacat gatggctgcc cttgatctct atttccagct
     1141 gtgctctgtg gaggttacct gtgagtcagg cagtgtcatg gcggccactc tggccaatgg
     1201 cggcatctgc cctatcacag gggagagcgt gctgagcgcc gaggccgtgc gcaacaccct
     1261 cagcctcatg cattcctgtg gcatgtatga cttctcgggc cagtttgcct tccatgtggg
     1321 cctgccagcc aagtcagctg tgtcaggagc catcctcctg gttgtaccca atgtcatggg
     1381 catgatgtgt ctgtcgccgc cgttagacaa gctggggaac agccaaaggg gcatcaactt
     1441 ctgccagaag ttggtgtctc tctttaactt ccacaactat gacaacctgc ggcactgcgc
     1501 tcggaagtta gacccacgga gggaaggagg agaggttcgg aacaagaccg tggtgaacct
     1561 gctatttgct gcatatagtg gagatgtctc ggcccttcga aggtttgcat tgtcggccat
     1621 ggatatggag cagaaggact atgattcccg cacagcccta catgtcgctg cagctgaagg
     1681 gcacattgaa gttgtcaaat ttctgatcga ggcttgcaaa gtgaatcctt ttgtcaagga
     1741 caggtggggc aacattcccc tggacgatgc tgtgcagttc aaccacctgg aggtggtcaa
     1801 actgcttcaa gattaccatg actcctactt gctctctgag actcaagctg aggcggcagc
     1861 tgagaccctg tccaaagaga atctagagag catggtatga gcacaggccc aggggcagtc
     1921 cctgcttcag aaaaagcatg agctggccac acatttaacc cacagccacc aaaaatacta
     1981 cggcaagctg cttccttggg gatcgacaca gccatttggt gacacaggcc agtgttttct
     2041 gagaatcaaa acgccccatc agttcagtgc acagcacaga gaaaagcctc aatggatacc
     2101 tgagcagagc tagctacaga gacctccaga tacagctcga gagtagcccg ggtctttacc
     2161 caggtcccct tttcctctct gaagaaatca tcatgccaac aagcaacttc cttggagagc
     2221 ctctagctac atgcatatgt atctgccgca gagtaagaga aatgggtgag agccaaacca
     2281 cgtggcttta gaagtctgcg gagatctgtc catcttagcc aggacacgct gctactgctg
     2341 acagcaattt tatagacaga gtattttgtg ttcaaataaa ctttaattac atgaattata
     2401 atttttattt tatattttgc taaatttagt cgttctcttt ctcccctaag gacatcatca
//
