LOCUS       NM_001081081            4974 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus glutaminase (Gls), transcript variant 1, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001081081 XM_129846
VERSION     NM_001081081.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Hu,G., Yu,Y., Ren,Y., Tower,R.J., Zhang,G.F. and Karner,C.M.
  TITLE     Glutaminolysis provides nucleotides and amino acids to regulate
            osteoclast differentiation in mice
  JOURNAL   EMBO Rep 25 (10), 4515-4541 (2024)
   PUBMED   39271775
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Li,M., Wang,Y., Wei,X., Cai,W.F., Wu,J., Zhu,M., Wang,Y., Liu,Y.H.,
            Xiong,J., Qu,Q., Chen,Y., Tian,X., Yao,L., Xie,R., Li,X., Chen,S.,
            Huang,X., Zhang,C., Xie,C., Wu,Y., Xu,Z., Zhang,B., Jiang,B.,
            Wang,Z.C., Li,Q., Li,G., Lin,S.Y., Yu,L., Piao,H.L., Deng,X.,
            Han,J., Zhang,C.S. and Lin,S.C.
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to
            glutamine
  JOURNAL   Cell Res 34 (10), 683-706 (2024)
   PUBMED   38898113
REFERENCE   3  (bases 1 to 4974)
  AUTHORS   Galvan,C., Flores,A.A., Cerrilos,V., Avila,I., Murphy,C., Zheng,W.,
            Christofk,H.R. and Lowry,W.E.
  TITLE     Defining metabolic flexibility in hair follicle stem cell induced
            squamous cell carcinoma
  JOURNAL   Sci Adv 10 (38), eadn2806 (2024)
   PUBMED   39303037
REFERENCE   4  (bases 1 to 4974)
  AUTHORS   Mercado-Gomez,M., Goikoetxea-Usandizaga,N., Kerbert,A.J.C.,
            Gracianteparaluceta,L.U., Serrano-Macia,M., Lachiondo-Ortega,S.,
            Rodriguez-Agudo,R., Gil-Pitarch,C., Simon,J., Gonzalez-Recio,I.,
            Fondevila,M.F., Santamarina-Ojeda,P., Fraga,M.F., Nogueiras,R.,
            Heras,J.L., Jalan,R., Martinez-Chantar,M.L. and Delgado,T.C.
  TITLE     The lipopolysaccharide-TLR4 axis regulates hepatic glutaminase 1
            expression promoting liver ammonia build-up as steatotic liver
            disease progresses to steatohepatitis
  JOURNAL   Metabolism 158, 155952 (2024)
   PUBMED   38906371
  REMARK    GeneRIF: The lipopolysaccharide-TLR4 axis regulates hepatic
            glutaminase 1 expression promoting liver ammonia build-up as
            steatotic liver disease progresses to steatohepatitis.
REFERENCE   5  (bases 1 to 4974)
  AUTHORS   Gubser,P.M., Wijesinghe,S., Heyden,L., Gabriel,S.S., de Souza,D.P.,
            Hess,C., McConville,M.M., Utzschneider,D.T. and Kallies,A.
  TITLE     Aerobic glycolysis but not GLS1-dependent glutamine metabolism is
            critical for anti-tumor immunity and response to checkpoint
            inhibition
  JOURNAL   Cell Rep 43 (8), 114632 (2024)
   PUBMED   39159042
REFERENCE   6  (bases 1 to 4974)
  AUTHORS   Colombo,M.P., Martinotti,A., Howard,T.A., Schneider,C.,
            D'Eustachio,P. and Seldin,M.F.
  TITLE     Localization of growth arrest-specific genes on mouse chromosomes
            1, 7, 8, 11, 13, and 16
  JOURNAL   Mamm Genome 2 (2), 130-134 (1992)
   PUBMED   1347472
REFERENCE   7  (bases 1 to 4974)
  AUTHORS   Levan,G., Szpirer,J., Szpirer,C., Klinga,K., Hanson,C. and
            Islam,M.Q.
  TITLE     The gene map of the Norway rat (Rattus norvegicus) and comparative
            mapping with mouse and man
  JOURNAL   Genomics 10 (3), 699-718 (1991)
   PUBMED   1889815
REFERENCE   8  (bases 1 to 4974)
  AUTHORS   Yasue,M., Serikawa,T. and Yamada,J.
  TITLE     Chromosomal assignments of 23 biochemical loci of the rat by using
            rat x mouse somatic cell hybrids
  JOURNAL   Cytogenet Cell Genet 57 (2-3), 142-148 (1991)
   PUBMED   1914521
REFERENCE   9  (bases 1 to 4974)
  AUTHORS   Howard,T.A., Rochelle,J.M. and Seldin,M.F.
  TITLE     Cd28 and Ctla-4, two related members of the Ig supergene family,
            are tightly linked on proximal mouse chromosome 1
  JOURNAL   Immunogenetics 33 (1), 74-76 (1991)
   PUBMED   1671668
REFERENCE   10 (bases 1 to 4974)
  AUTHORS   Mock,B., Kozak,C., Seldin,M.F., Ruff,N., D'Hoostelaere,L.,
            Szpirer,C., Levan,G., Seuanez,H., O'Brien,S. and Banner,C.
  TITLE     A glutaminase (gis) gene maps to mouse chromosome 1, rat chromosome
            9, and human chromosome 2
  JOURNAL   Genomics 5 (2), 291-297 (1989)
   PUBMED   2571577
  REMARK    Erratum:[Genomics 1989 Nov;5(4):957]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123752.8.
            
            On Jan 11, 2008 this sequence version replaced NM_001081081.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927934.2150183.1,
                                           SRR7974084.7826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-641               AC123752.8         32862-33502
            642-738             AC123752.8         46055-46151
            739-860             AC123752.8         46485-46606
            861-990             AC123752.8         50400-50529
            991-1070            AC123752.8         51537-51616
            1071-1234           AC123752.8         53728-53891
            1235-1293           AC123752.8         58735-58793
            1294-1326           AC123752.8         66207-66239
            1327-1385           AC123752.8         66325-66383
            1386-1452           AC123752.8         69285-69351
            1453-1503           AC123752.8         69879-69929
            1504-1680           AC123752.8         74807-74983
            1681-1812           AC123752.8         76540-76671
            1813-1905           AC123752.8         77323-77415
            1906-1967           AC123752.8         97136-97197
            1968-2044           AC123752.8         97533-97609
            2045-2108           AC123752.8         97695-97758
            2109-4974           AC123752.8         99780-102645
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 26.86 cM"
     gene            1..4974
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /note="glutaminase"
                     /db_xref="GeneID:14660"
                     /db_xref="MGI:MGI:95752"
     exon            1..641
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..2265
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /EC_number="3.5.1.2"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="glutaminase kidney isoform, mitochondrial
                     isoform 1"
                     /protein_id="NP_001074550.1"
                     /db_xref="CCDS:CCDS35561.1"
                     /db_xref="GeneID:14660"
                     /db_xref="MGI:MGI:95752"
                     /translation="MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRP
                     TAGLVAAARLHPWWGGGGRAKGPGAGGLSSSPSEILQELGKGGTPPQQQQQQQQQPGA
                     SPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQGLLPSLEDLLFYTIAEGQEKIP
                     VHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQ
                     AFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQ
                     RHSIGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKP
                     HNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDR
                     NFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGE
                     RVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSP
                     PLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAA
                     YTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRW
                     NNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDDGKGNQTVHKNLDGLL"
     exon            642..738
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            739..860
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            861..990
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1070
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1234
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1293
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1326
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1385
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1452
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1503
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1680
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1812
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1905
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..1967
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2044
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2108
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..4974
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4950..4955
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4974
                     /gene="Gls"
                     /gene_synonym="6330442B14; B230365M23Rik"
                     /note="major polyA site"
ORIGIN      
        1 tgagcgtcag tctcagtgcg gagctcgcgg cggccagagc aacttcggct ggctgcaggc
       61 tgggcggcgg cggcggcgag ggcggcggcg ttggcggcgg gagcggcgga gctggggcca
      121 caccccgtcc cggacttttt ctctttccag tcctctcccc gccgtgcggg acacggttcc
      181 gggagcagag cggccgccca cgccccaagc atcctcatct gacgagcggg cgccggcggt
      241 atgatgcggc tgcgaggctc ggcgatgcta cgggagctgc tcttaaggcc gcccgccgcc
      301 gtcggagccg tcctgcggcg cgcgcagccc ctcggcacgc tgtgccggcg cccccggggc
      361 gggagccggc cgaccgctgg cctggtggcc gctgcgcgac tccacccgtg gtggggcggg
      421 ggcggccgcg cgaagggccc cggcgcgggc ggcctgtcca gttcgccctc ggagatccta
      481 caggagctgg ggaagggggg tacgccgcca cagcagcagc agcagcagca gcagcagcca
      541 ggggcgtcgc cacccgcagc cccgggcccc aaggacagcc cgggggagac cgacgcgttc
      601 ggcaacagcg agggcaagga gatggtggcc gcgggcgaca ataaaataaa acagggtctg
      661 ttacctagct tggaagattt gctgttctat acaattgcag aaggacaaga aaagatacct
      721 gttcacaagt ttattacagc actcaaatct acaggattgc gaacatctga tcccaggttg
      781 aaagagtgta tggatatgtt aagattaact cttcagacaa cgtcagatgg tgtcatgcta
      841 gacaaagatc tttttaaaaa gtgtgttcaa agcaacattg ttttgttgac acaagcattt
      901 agaagaaagt ttgtcattcc tgactttatg tcttttacct cacacatcga tgagttatat
      961 gaaagtgcta aaaagcagtc tggagggaag gttgctgatt atattcctca gctggccaaa
     1021 ttcagtcctg atttgtgggg tgtatctgtc tgtactgtag atgggcaaag gcattctatt
     1081 ggagatacca aagttccttt ttgtcttcag tcctgtgtaa aacccctgaa atatgcaatt
     1141 gctgttaatg acctgggaac tgagtatgta catcgctatg ttgggaagga gccaagtgga
     1201 ttaagattca acaaactctt tttgaatgaa gatgataaac cacataatcc tatggtaaat
     1261 gctggagcaa ttgttgtgac ttctctaata aagcaaggag taaataatgc tgagaagttt
     1321 gactacgtga tgcaattttt gaataagatg gctggtaatg aatatgttgg attcagtaat
     1381 gcaacgtttc agtctgaacg agaaagtgga gatcgaaatt ttgcaatagg atattactta
     1441 aaagaaaaga agtgttttcc agaaggcaca gacatggttg ggatactaga tttttacttc
     1501 cagctgtgct ctattgaagt gacatgtgaa tcagcaagtg tgatggctgc caccttggct
     1561 aatggtggtt tctgcccaat tactggtgaa agagtcctca gtcctgaggc agttcggaat
     1621 acactgagct tgatgcattc ttgtggcatg tatgacttct cagggcagtt tgcgttccat
     1681 gttggtcttc ctgcaaaatc tggagttgct gggggtattc ttttagttgt ccccaacgtc
     1741 atgggcatga tgtgttggtc tcctcctctt gacaagatgg gcaacagtgt taagggaatt
     1801 cacttttgtc acgatcttgt ttctctgtgt aacttccata actatgataa tttgagacac
     1861 tttgcaaaaa aacttgatcc tcggagagaa ggaggtgatc aaagggtgaa gtcggtgata
     1921 aaccttctgt ttgccgcata cactggagat gtgtctgccc tccgaaggtt tgctctgtca
     1981 gccatggaca tggagcagcg ggattatgac tccagaacag ccctccatgt cgcagcagca
     2041 gagggtcatg ttgaagttgt caagtttttg ctggaagctt gcaaagtaaa ccctttcccc
     2101 aaggacaggt ggaataatac ccccatggat gaagcactac actttggaca ccatgatgtt
     2161 tttaaaatcc ttcaggaata ccaagttcag tacacacctc aaggggattc tgatgatgga
     2221 aagggaaacc agactgtcca caagaatctc gacgggttgc tataatggtc tgcaccccaa
     2281 gacttccatt acttacctag tcattgtgga acatgactat ggagagcatt gtatatttct
     2341 atctggtagt aatgtgtatt tacaacatct gtcactgcag tgttaccgga gcttccttca
     2401 ttgtgcgcac acgacaaatc tgagttcttt gggaaaaaaa tagaaatgaa gcagcctccc
     2461 ttcataatgt gagcaatagt tacctcgtgc attgtacaat gtggtgtaaa agagtagtta
     2521 ccaatgccag ctgaactgtg tggtcttcat ggtttgcgtt ctgtacattt tcaagccctg
     2581 gtgatgatac gctcatatgc acttaggagt gagctttgtt gtacagtctg tccacggggt
     2641 cgatgctgtt attaggtgaa aatagtgtga tctttaagac tttaaataca gatttagttt
     2701 tgagtgtttg agagaccact acacttgtat ggttgagtgt ttaaaatgtc tatcaccctc
     2761 acttcagagt gtgactcttt aaatattaaa atagatacta actgttcata gaacaggccg
     2821 attctgatta gattttatca gggaatctgt taagatatgt ttggtgacca aaacgtatgt
     2881 gtgaatatag ttctagcact tttaaatttt tcctttccat acaacgcttg ggccagcctc
     2941 tctgtgctgc gtggctgtcg gtccccctca gctgggaaag agagcactgg ctcactgtgc
     3001 agttttcatg tttcctcagc aagccatcaa gcctcacatc tctaccatca gagatagagc
     3061 ttggccattt atctaaggaa gatgagccaa aattatgaca tctaaaataa tcgtcagtct
     3121 taagagtaaa gacagcgaaa ctgcacactt ataagttctt ttcagcttct acaataaaga
     3181 aaagttcaga aatgctttca gttaccaaag ttataacgat atatttagga aaagctacaa
     3241 ataacactta ctttgaatcc tgctgtcaaa tgtctgcatc aagatagcac ccctttgtgg
     3301 gaggccctga gtatcttctc ttcctctact gcctaactgt tggtgggctg tatcattcaa
     3361 taagatcact tcattttcaa cttagaccca ccgtttcttt tttgttgttt tgttttgttt
     3421 ggtttggttt ggttgggttg ggttgttgtt tttggttttt tcgagacagg gtttctctgt
     3481 gtagccctgg ctgtcctgga actcactttg tagaccaggc tggccttgaa ctcagaaatc
     3541 cgcctgcctc tgcctcccga gtgctgggat taaaggtgca aactaccacg cctggcagac
     3601 ccaccatttc tttgctttgg aaaggtaatt tatgattaac ttagataata ggtaaaagcg
     3661 accttacaaa aaacataatt atctaggagt cccacatact ggacctaccc tattatacct
     3721 ccaagagata aagggtatgt tagtgaggac ttttgcacac aagtgcatgc acacttggca
     3781 tacacacaca cacacacaca cacacacaca cacacacaca cacacggact tcttggaaac
     3841 tgctttatga agaaactgct ttatgaaata agcaaaattc tcaagtgcac agatactagc
     3901 agttatgaca gtaatacagc gtcttctgtg accctcacta cctgcactgc ttgcatccct
     3961 gctttatgcc tggtggcaca ttattcaccc ggtaacctcc agctgctttg atcctgtttc
     4021 agtcaaagtc agcttcagcc accccctcca ttccctagcc agctccaccc ttgatgaaac
     4081 tgtggctaat gttccttcac taggacaggc accatgagtg tgtttctaag ttccagagtc
     4141 tgtggggagg atggtgggtg ggcagccagc cctgttgcta tgttgcttct tccacacccc
     4201 ctcaagacag gtgcataggt ggcactggga acatcctacg cagggacaac ctccaaaatt
     4261 aatgggtgaa catggttttt ttggaatcaa ctgagataat gctatttcaa tagcggctgg
     4321 ctttttgtga ttcagtaact taaatattgc cagtgactga ggatcccctc cagtcatggt
     4381 tctgtatatt ctttgagaca ggtgttttca tcttctctca gctcagtgct gttttgtaca
     4441 gtctctgtgg cttggttgag tatgctcttt cctgtgccag gtcttgctct ggctgttcgc
     4501 tactggctga taataacaag gaccctgtgt gtgtgtgaat gagccgctaa ctgctaccat
     4561 ctgtaaactc caaagatctg tttgttttgg ctttacaatc ttagctaatt tttctgtatc
     4621 ctggaaccat tacatgatca tgttgctttg aagatctttt tatgccactg tttctgctgt
     4681 cttggttctg acacccctgt ctggtgatat gctatacccc agtgctgcct acacgtgctt
     4741 tagctgtaga gctgggtata ctgttgatcc agctgtccgt cagggacttg ataacctgat
     4801 gtttgatgta gatccctgct ggggagtcca caactatgaa tgtatttact tccaacattt
     4861 cccaaaatga aaactataaa ttgcaagtat tctggaattg ggaaatactt attttaaatg
     4921 agatcaggta gtgttgcttt ttacagcata ataaatatgt gtattgaaaa caaa
//
