LOCUS       NM_001345955            3086 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus glycine receptor, beta subunit (Glrb), transcript
            variant 1, mRNA.
ACCESSION   NM_001345955
VERSION     NM_001345955.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Wiessler,A.L., Hasenmuller,A.S., Fuhl,I., Mille,C., Cortes
            Campo,O., Reinhard,N., Schenk,J., Heinze,K.G., Schaefer,N.,
            Specht,C.G. and Villmann,C.
  TITLE     Role of the Glycine Receptor beta Subunit in Synaptic Localization
            and Pathogenicity in Severe Startle Disease
  JOURNAL   J Neurosci 44 (2), e0837232023 (2024)
   PUBMED   37963764
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3086)
  AUTHORS   Rivares,C., Vignaud,A., Noort,W., Koopmans,B., Loos,M.,
            Kalinichev,M. and Jaspers,R.T.
  TITLE     Glycine receptor subunit-beta-deficiency in a mouse model of
            spasticity results in attenuated physical performance, growth, and
            muscle strength
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 322 (5), R368-R388 (2022)
   PUBMED   35108108
REFERENCE   4  (bases 1 to 3086)
  AUTHORS   Winkelmann,A., You,X., Grunewald,N., Haussler,U., Krestel,H.,
            Haas,C.A., Schwarz,G., Chen,W. and Meier,J.C.
  TITLE     Identification of a new genomic hot spot of evolutionary
            diversification of protein function
  JOURNAL   PLoS One 10 (5), e0125413 (2015)
   PUBMED   25955356
  REMARK    GeneRIF: A species-specific thymine-to-guanine sequence variation
            in the Glrb gene has been identified which gives rise to
            species-specific splice donor sites in the Glrb genes of mouse and
            bushbaby.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3086)
  AUTHORS   Lynch,J.W.
  TITLE     Native glycine receptor subtypes and their physiological roles
  JOURNAL   Neuropharmacology 56 (1), 303-309 (2009)
   PUBMED   18721822
  REMARK    Review article
REFERENCE   6  (bases 1 to 3086)
  AUTHORS   Oertel,J., Villmann,C., Kettenmann,H., Kirchhoff,F. and Becker,C.M.
  TITLE     A novel glycine receptor beta subunit splice variant predicts an
            unorthodox transmembrane topology. Assembly into heteromeric
            receptor complexes
  JOURNAL   J Biol Chem 282 (5), 2798-2807 (2007)
   PUBMED   17145751
  REMARK    GeneRIF: Transcripts are detected in cerebral cortex, liver, and
            heart. Lack of exon 7 results in a profoundly altered prediction of
            transmembrane topology.
REFERENCE   7  (bases 1 to 3086)
  AUTHORS   Kingsmore,S.F., Giros,B., Suh,D., Bieniarz,M., Caron,M.G. and
            Seldin,M.F.
  TITLE     Glycine receptor beta-subunit gene mutation in spastic mouse
            associated with LINE-1 element insertion
  JOURNAL   Nat Genet 7 (2), 136-141 (1994)
   PUBMED   7920630
REFERENCE   8  (bases 1 to 3086)
  AUTHORS   Stone,C. and Pinto,L.H.
  TITLE     Receptive field organization of retinal ganglion cells in the
            spastic mutant mouse
  JOURNAL   J Physiol 456, 125-142 (1992)
   PUBMED   1338094
REFERENCE   9  (bases 1 to 3086)
  AUTHORS   Becker,C.M., Schmieden,V., Tarroni,P., Strasser,U. and Betz,H.
  TITLE     Isoform-selective deficit of glycine receptors in the mouse mutant
            spastic
  JOURNAL   Neuron 8 (2), 283-289 (1992)
   PUBMED   1371219
REFERENCE   10 (bases 1 to 3086)
  AUTHORS   Eicher,E.M. and Womack,J.E.
  TITLE     Chromosomal location of soluble glutamic-pyruvic transaminase-1
            (Gpt-1) in the mouse
  JOURNAL   Biochem Genet 15 (1-2), 1-8 (1977)
   PUBMED   849243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121908.3.
            
            On Jul 28, 2022 this sequence version replaced NM_001345955.1.
            
            Summary: This gene encodes the beta subunit of the glycine
            receptor, which is a pentamer composed of alpha and beta subunits.
            The receptor functions as a neurotransmitter-gated ion channel,
            which produces hyperpolarization via increased chloride conductance
            due to the binding of glycine to the receptor. This gene is
            transcribed throughout the central nervous system of neonatal and
            adult mice. In humans, mutations in this gene cause startle
            disease, also known as hereditary hyperekplexia or congenital
            stiff-person syndrome, a disease characterized by muscular
            rigidity. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784645.386898.1,
                                           SRR1660817.312997.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC121908.3         148186-148319       c
            135-285             AC121908.3         146637-146787       c
            286-392             AC121908.3         114295-114401       c
            393-460             AC121908.3         112678-112745       c
            461-690             AC121908.3         96561-96790         c
            691-773             AC121908.3         96372-96454         c
            774-914             AC121908.3         94830-94970         c
            915-1067            AC121908.3         90454-90606         c
            1068-1360           AC121908.3         85577-85869         c
            1361-1432           AC121908.3         85097-85168         c
            1433-3086           AC121908.3         78273-79926         c
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 35.71 cM"
     gene            1..3086
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /note="glycine receptor, beta subunit"
                     /db_xref="GeneID:14658"
                     /db_xref="MGI:MGI:95751"
     exon            1..134
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            135..285
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..1726
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; glycine receptor subunit beta; inhibitory
                     glycine receptor; glycine receptor 58 kDa subunit"
                     /codon_start=1
                     /product="glycine receptor subunit beta isoform 1
                     precursor"
                     /protein_id="NP_001332884.1"
                     /db_xref="GeneID:14658"
                     /db_xref="MGI:MGI:95751"
                     /translation="MKFSLAISFFILMSLLFEDACAKEKSSKKGKGKKKQYLCPSQQS
                     PEDLARVPPNSTSNILNRLLVSYDPRIRPNFKGIPVDVVVNIFINSFGSIQETTMDYR
                     VNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWKPDLFFANEKSANFHDVTQEN
                     ILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLRFIWQSG
                     DPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAP
                     TLLIVVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLI
                     ACLLFGFASLVEYAVVQVMLNNPKRVEAEKARIAKAEQADGKGGNAAKKNTVNGTGTP
                     VHISTLQELPMVCQQITYQLDGWPTDTLTTQVGETRCKKVCTSKSDLRSNDFSIVGSL
                     PRDFELSNYDCYGKPIEVNNGLGKPQAKNKKPPPAKPVIPTAAKRIDLYARALFPFCF
                     LFFNVIYWSIYL"
     exon            286..392
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            393..460
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            461..690
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            691..773
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            774..914
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1067
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1360
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1432
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..3086
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3065..3070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /note="hexamer: ATTAAA"
     polyA_site      3086
                     /gene="Glrb"
                     /gene_synonym="Glyrb; spa; spastic"
                     /note="major polyA site"
ORIGIN      
        1 aggcgcgtca aactcggcgg cggcggcggt ggcggcggtg gcgcgggcgg cggcgggact
       61 cgctggtcgc agctgcctct gcgcccgcca cctgcgccgc cgtcacctcg gcggagggct
      121 gaggggcgcg tgcgattgag ctgctgaaac ttccgttttc aagatgaagt tttcgttggc
      181 aatctccttt tttattttaa tgtccttgtt gtttgaagac gcttgtgcta aagaaaagtc
      241 ttccaagaaa gggaagggga aaaagaagca gtacttgtgc ccatctcagc agtcacccga
      301 ggacctggcc cgtgtgcccc ccaactccac cagcaatatc ttgaacaggc tgctggtcag
      361 ttatgatccc aggatcagac caaacttcaa aggcattcct gttgatgtag tagtcaacat
      421 ttttattaat agttttggat ccattcaaga gacaacaatg gactatagag ttaacatttt
      481 cttgagacag aaatggaatg accccagact caagctacct agtgacttca gaggctcaga
      541 tgcactgaca gttgacccca ccatgtataa gtgcttgtgg aaacctgact tattctttgc
      601 aaatgaaaaa agtgccaatt ttcatgatgt gacccaagaa aatatcctgt tgtttatctt
      661 tcgggatgga gacgtccttg tgagcatgag gttgtctatt acactttcat gtcctctaga
      721 cttaactctg tttcccatgg acacacaacg ctgcaaaatg caacttgaga gctttggata
      781 tacaaccgat gatttaagat tcatctggca gtcaggagat cctgttcagt tggaaaaaat
      841 tgctttacct caatttgata ttaaaaagga ggatatcgaa tatggcaact gtacaaaata
      901 ctataaaggc actggttact acacttgtgt ggaggtcatc ttcaccctga ggagacaggt
      961 tgggttctac atgatgggcg tatatgcacc aaccttgctg attgtggttc tctcctggct
     1021 ctctttctgg atcaaccctg atgctagtgc tgccagagta cctctgggca tcttctccgt
     1081 gctcagtttg gcctcagagt gcaccaccct cgcagccgag cttcctaaag tgtcttatgt
     1141 gaaggcgctg gatgtgtggc tcattgcctg cctgctcttc gggtttgcct ccctcgtgga
     1201 gtacgctgtg gtccaggtga tgctgaacaa tcccaaaagg gttgaagccg agaaggcccg
     1261 aatagctaag gctgagcaag cagatgggaa aggtggaaac gcagctaaga agaatactgt
     1321 aaacggcacg gggacccctg ttcatatcag cactttgcag gagcttccaa tggtttgtca
     1381 gcagatcact taccaactgg atggctggcc cacagacacg ctcaccacac aggttggtga
     1441 gaccagatgc aaaaaagttt gtacttccaa gtctgatttg agatccaatg acttcagcat
     1501 tgttggaagc ttaccaagag attttgaatt atccaattat gactgctatg ggaaacccat
     1561 cgaagtcaac aatggacttg ggaaaccaca ggcaaagaac aagaagcctc cgcctgccaa
     1621 gcctgtcatc ccaacagcag ccaagcgcat cgacctttat gcaagagcat tatttccttt
     1681 ctgcttcttg ttcttcaatg ttatatattg gtctatatat ttatgataaa ccatttccct
     1741 ttgtatgaaa tacagcacca tttcattgtg accaatgcaa ttcataattg ctgatctgtg
     1801 gaaactttgc attttcctag caatatattg cattttggat gccacttgat tataataaaa
     1861 tacagcacct tacttttgaa tggcagcatg accctgtaaa gtctgtgcac cgactgtgag
     1921 atagatagat agatgtatgt atattgagca tagtgcttta agaatcaatg acaaatatgc
     1981 atactatgta atctacataa tgtgctcttc agctactgta actctgtaac tacaaatact
     2041 tccagtaatc cagatgaaaa cataatgtgc acgctgaccc ctgtcatgtt cactggtcca
     2101 tgtatgcagt atcttacatc cccttccttg taagaaagaa agccatctta ttaaataatt
     2161 ttcgatagta ttattgcaga ttaagcaaga ttatatggca tagtgtttaa agtttttaag
     2221 agaggtagat cagtaatagt tatgcaggct ccttaagaaa taaaatcata attatacatt
     2281 tataaagcaa gcttattttt aagtgtgctt gtgtcgtcaa aatgccagat aatagaacag
     2341 taagcatttc tgaagaaaag agaaatctgg gctagtataa atgcatagca agttctggct
     2401 tctggcaaaa tacaaatagt gatagatgta ttaattaaat attttggata aaataaatcc
     2461 tttaatccac cttaaatcaa agtctatttt catgcctttc attggttggt atggagagca
     2521 ttaagcataa gccttaaaaa gagttgcaca catttcaaac ctaaatagtg tggtgacatg
     2581 tgagtccaga ttactgttag actccagaaa ttgttagcat gaacttgaaa tggaaaaatc
     2641 ataatcactg aaggacttag gttgtttatt ttattaaaat gtttctatat ttcaagtaat
     2701 attcaaactg tttttggaat tttttttatt tatttgaatg tatttgaata tgttacaaaa
     2761 caatgattta gttaataatt ttttttattt aacctttaga cttcaccctt ttcttctcgc
     2821 tgaaagtcta gatattgggg ctggctgact tcatcttatt tctatctgaa aagaacccct
     2881 aagcctcttc ttataaagcc ccattttcta acagtgtcat agtgtaactg tttatttctg
     2941 tcttagctaa aattggagct attagtaatt actgtaatgt aaacagacca taaagccatt
     3001 ttgcgttatt tggagtgctt ttgttcttgt tgatagtttg tacagccaac tcataacata
     3061 atgcattaaa agtattattt gtgcaa
//
