LOCUS       NM_020003               1557 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus glycosylated lysosomal membrane protein (Glmp), mRNA.
ACCESSION   NM_020003
VERSION     NM_020003.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Antwi,M.B., Dumitriu,G., Simon-Santamaria,J., Romano,J.S., Li,R.,
            Smedsrod,B., Vik,A., Eskild,W. and Sorensen,K.K.
  TITLE     Liver sinusoidal endothelial cells show reduced scavenger function
            and downregulation of Fc gamma receptor IIb, yet maintain a
            preserved fenestration in the Glmpgt/gt mouse model of slowly
            progressing liver fibrosis
  JOURNAL   PLoS One 18 (11), e0293526 (2023)
   PUBMED   37910485
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Lopez,D.M., Kahlau,L., Jungnickel,K.E.J., Low,C. and Damme,M.
  TITLE     Characterization of the complex of the lysosomal membrane
            transporter MFSD1 and its accessory subunit GLMP
  JOURNAL   FASEB J 34 (11), 14695-14709 (2020)
   PUBMED   32959924
  REMARK    GeneRIF: Characterization of the complex of the lysosomal membrane
            transporter MFSD1 and its accessory subunit GLMP.
REFERENCE   3  (bases 1 to 1557)
  AUTHORS   Massa Lopez,D., Thelen,M., Stahl,F., Thiel,C., Linhorst,A.,
            Sylvester,M., Hermanns-Borgmeyer,I., Lullmann-Rauch,R., Eskild,W.,
            Saftig,P. and Damme,M.
  TITLE     The lysosomal transporter MFSD1 is essential for liver homeostasis
            and critically depends on its accessory subunit GLMP
  JOURNAL   Elife 8, e50025 (2019)
   PUBMED   31661432
  REMARK    GeneRIF: Mfsd1 knockout mice develop splenomegaly and severe liver
            disease. Proteomics of isolated lysosomes from Mfsd1 knockout mice
            revealed GLMP as a critical accessory subunit for MFSD1. MFSD1 and
            GLMP physically interact. GLMP is essential for the maintenance of
            normal levels of MFSD1 in lysosomes and vice versa.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1557)
  AUTHORS   Kong,X.Y., Feng,Y.Z., Eftestol,E., Kase,E.T., Haugum,H., Eskild,W.,
            Rustan,A.C. and Thoresen,G.H.
  TITLE     Increased glucose utilization and decreased fatty acid metabolism
            in myotubes from Glmp(gt/gt) mice
  JOURNAL   Arch Physiol Biochem 122 (1), 36-45 (2016)
   PUBMED   26707125
  REMARK    GeneRIF: the importance of GLMP in skeletal muscle
REFERENCE   5  (bases 1 to 1557)
  AUTHORS   Kong,X.Y., Kase,E.T., Herskedal,A., Schjalm,C., Damme,M.,
            Nesset,C.K., Thoresen,G.H., Rustan,A.C. and Eskild,W.
  TITLE     Lack of the Lysosomal Membrane Protein, GLMP, in Mice Results in
            Metabolic Dysregulation in Liver
  JOURNAL   PLoS One 10 (6), e0129402 (2015)
   PUBMED   26047317
  REMARK    GeneRIF: Data indicate that increased flux of glucose, increased de
            novo lipogenesis and lipid accumulation were detected in lysosomal
            protein NCU-G1 (Glmp) gt/gt primary hepatocytes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1557)
  AUTHORS   Kong,X.Y., Nesset,C.K., Damme,M., Loberg,E.M., Lubke,T.,
            Maehlen,J., Andersson,K.B., Lorenzo,P.I., Roos,N., Thoresen,G.H.,
            Rustan,A.C., Kase,E.T. and Eskild,W.
  TITLE     Loss of lysosomal membrane protein NCU-G1 in mice results in
            spontaneous liver fibrosis with accumulation of lipofuscin and iron
            in Kupffer cells
  JOURNAL   Dis Model Mech 7 (3), 351-362 (2014)
   PUBMED   24487409
  REMARK    GeneRIF: Microscopic examination of liver sections revealed
            accumulation of autofluorescent lipofuscin and iron in
            Ncu-g1(gt/gt) Kupffer cells.
REFERENCE   7  (bases 1 to 1557)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   8  (bases 1 to 1557)
  AUTHORS   Schieweck,O., Damme,M., Schroder,B., Hasilik,A., Schmidt,B. and
            Lubke,T.
  TITLE     NCU-G1 is a highly glycosylated integral membrane protein of the
            lysosome
  JOURNAL   Biochem J 422 (1), 83-90 (2009)
   PUBMED   19489740
  REMARK    GeneRIF: The lysosomal localization of the mouse orthologue of the
            human C1orf85 protein, which has been termed kidney-predominant
            protein NCU-G1, is demonstrated.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1557)
  AUTHORS   Kawamura,T., Kuroda,N., Kimura,Y., Lazoura,E., Okada,N. and
            Okada,H.
  TITLE     cDNA of a novel mRNA expressed predominantly in mouse kidney
  JOURNAL   Biochem Genet 39 (1-2), 33-42 (2001)
   PUBMED   11444019
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB027142.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB027142.1, AK154340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="BALB/c"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.8 cM"
     gene            1..1557
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /note="glycosylated lysosomal membrane protein"
                     /db_xref="GeneID:56700"
                     /db_xref="MGI:MGI:1913318"
     exon            1..179
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1274
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /note="lysosomal protein NCU-G1"
                     /codon_start=1
                     /product="glycosylated lysosomal membrane protein
                     precursor"
                     /protein_id="NP_064387.1"
                     /db_xref="CCDS:CCDS17469.1"
                     /db_xref="GeneID:56700"
                     /db_xref="MGI:MGI:1913318"
                     /translation="MFRCWGPHWGWVPCAPTPWLLLSLLVCSAPFGLQGEETRQVSME
                     VISGWPNPQNLLHIRAVGSNSTLHYVWSSLGPPAVVLVATNTTQSVLSVNWSLLLSPD
                     PAGALMVLPKSSIQFSSALVFTRLLEFDSTNASEGAQPPGKPYPPYSLAKFSWNNITN
                     SLDLANLSADFQGRPVDDPTGAFANGSLTFKVQAFSRSGRPAQPPRLLHTADVCQLEV
                     ALVGASPRGNHSLFGLEVATLGQGPDCPSVNERNSIDDEYAPAVFQLNQLLWGSSPSG
                     FMQWRPVAFSEEERARESALPCQASTLHSTLASSLPHSPIVQAFFGSQNNFCAFNLTF
                     GAPTGPGYWDQYYLCWSMLLGMGFPPVDIFSPLVLGIMAVALGAPGLMFLGGGLFLLL
                     RHRRYSEYQSIN"
     exon            180..434
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            435..632
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            633..851
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1108
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1537
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1514..1519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /note="hexamer: AATAGA"
     polyA_site      1537
                     /gene="Glmp"
                     /gene_synonym="0610031J06Rik; NCU-G1"
                     /note="major polyA site"
ORIGIN      
        1 caggaaagga gtcatgtgac tctgaggctc ttccgctgag tttaactagc tgtggctgta
       61 tgtttcgctg ttggggacct cactggggct gggtcccctg tgcccctacc ccatggctcc
      121 ttttgagtct acttgtgtgt tcagctccat ttggcctgca gggagaagag actcgccagg
      181 tatctatgga ggttatttcc ggctggccaa atccacagaa cctgcttcat attcgggcag
      241 tgggcagcaa ctccaccctg cactatgtgt ggagcagcct ggggcctcca gctgtggtgc
      301 tggtggccac taacaccact cagagtgtcc tgagtgtcaa ctggagtctc ctgctgtctc
      361 ccgatcctgc tggggccctg atggtgctcc ccaagagcag catacagttt tcttctgctc
      421 tcgtcttcac caggctgctt gagtttgaca gcaccaatgc gtccgagggg gcacagcctc
      481 caggaaagcc ataccctccg tactccttgg ccaagttctc ctggaacaat atcaccaact
      541 cattggatct tgccaacctg agtgccgatt ttcaaggccg ccctgtagat gatcctactg
      601 gagcattcgc caatggcagc ctgaccttca aggtccaagc cttttctaga tctggtcgac
      661 ctgctcagcc ccctcgcctc ctgcacacag cagatgtctg ccagttagaa gtggccctgg
      721 ttggagcttc tcctcgtgga aaccattccc tatttggtct agaggtagcc accttgggac
      781 agggccctga ctgcccctca gtgaacgaac ggaactccat cgatgatgaa tatgcacctg
      841 ccgtcttcca gctgaaccag ctgttatggg gctcttctcc atctggcttc atgcaatggc
      901 gaccagtggc tttctctgag gaggagcggg cccgagaatc agccctcccc tgccaggctt
      961 ccactcttca ctccaccttg gcatcttctc tccctcattc accaattgtt caagccttct
     1021 tcgggtccca gaacaacttc tgtgccttca atctgacctt tggggctccc acgggccctg
     1081 gctactggga ccaatactac ctttgctggt caatgcttct gggcatgggc ttccctccgg
     1141 tggatatctt ctctccacta gtcctcggaa tcatggcggt agccctggga gccccaggac
     1201 tcatgttcct ggggggaggc ctatttctgc tgctgcgcca caggcggtat tctgagtacc
     1261 agtccataaa ctgaaaccca ctctccagag ggaagggcag tgtctcagac tgtcggaggg
     1321 tggggggccc agggtcatga tctgcttttc tgcagaacct cagaagccag cctcaaccta
     1381 ctggggtgct cccagtaagt tgtccttcca aacccacgga gaggccacgc tcacgggttc
     1441 ccaaactccc gtttcctgtg gatgaaactt gaaggcctac atgagtgact gttggctgcc
     1501 ctaaggggca tgaaatagac atttccccca tacaagaaaa aaaaaaaaaa aaaaaaa
//
