LOCUS       NM_033320               4853 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus glucuronyl C5-epimerase (Glce), transcript variant 1,
            mRNA.
ACCESSION   NM_033320 XM_006511571
VERSION     NM_033320.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   He,F., Jiang,H., Peng,C., Wang,T., Xiao,R., Chen,M., Song,N.,
            Du,Z., Wang,H., Ding,X., Shao,Y., Fang,J., Zang,Y., Hua,R., Li,J.
            and Ding,K.
  TITLE     Hepatic glucuronyl C5-epimerase combats obesity by stabilising
            GDF15
  JOURNAL   J Hepatol 79 (3), 605-617 (2023)
   PUBMED   37217020
  REMARK    GeneRIF: Hepatic glucuronyl C5-epimerase combats obesity by
            stabilising GDF15.
REFERENCE   3  (bases 1 to 4853)
  AUTHORS   Patel,V.N., Pineda,D.L., Berenstein,E., Hauser,B.R., Choi,S.,
            Prochazkova,M., Zheng,C., Goldsmith,C.M., van Kuppevelt,T.H.,
            Kulkarni,A., Song,Y., Linhardt,R.J., Chibly,A.M. and Hoffman,M.P.
  TITLE     Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to
            reduce epithelial morphogenesis and adult salivary gland function
  JOURNAL   Matrix Biol 103-104, 37-57 (2021)
   PUBMED   34653670
REFERENCE   4  (bases 1 to 4853)
  AUTHORS   Cui,H., Cheng,X., Batool,T., Zhang,X. and Li,J.P.
  TITLE     Glucuronyl C5-epimerase is crucial for epithelial cell maturation
            during embryonic lung development
  JOURNAL   Glycobiology 31 (3), 223-230 (2021)
   PUBMED   32651954
  REMARK    GeneRIF: Glucuronyl C5-epimerase is crucial for epithelial cell
            maturation during embryonic lung development.
REFERENCE   5  (bases 1 to 4853)
  AUTHORS   Najas,S., Pijuan,I., Esteve-Codina,A., Usieto,S., Martinez,J.D.,
            Zwijsen,A., Arbones,M.L., Marti,E. and Le Dreau,G.
  TITLE     A SMAD1/5-YAP signalling module drives radial glia
            self-amplification and growth of the developing cerebral cortex
  JOURNAL   Development 147 (13) (2020)
   PUBMED   32541003
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4853)
  AUTHORS   Ledin,J., Staatz,W., Li,J.P., Gotte,M., Selleck,S., Kjellen,L. and
            Spillmann,D.
  TITLE     Heparan sulfate structure in mice with genetically modified heparan
            sulfate production
  JOURNAL   J Biol Chem 279 (41), 42732-42741 (2004)
   PUBMED   15292174
  REMARK    GeneRIF: NDST-1 and C5-epimerase have roles in heparan sulfate
            structure alterations in mouse tissue
REFERENCE   7  (bases 1 to 4853)
  AUTHORS   Li,J.P., Gong,F., Hagner-McWhirter,A., Forsberg,E., Abrink,M.,
            Kisilevsky,R., Zhang,X. and Lindahl,U.
  TITLE     Targeted disruption of a murine glucuronyl C5-epimerase gene
            results in heparan sulfate lacking L-iduronic acid and in neonatal
            lethality
  JOURNAL   J Biol Chem 278 (31), 28363-28366 (2003)
   PUBMED   12788935
REFERENCE   8  (bases 1 to 4853)
  AUTHORS   Pinhal,M.A., Smith,B., Olson,S., Aikawa,J., Kimata,K. and Esko,J.D.
  TITLE     Enzyme interactions in heparan sulfate biosynthesis: uronosyl
            5-epimerase and 2-O-sulfotransferase interact in vivo
  JOURNAL   Proc Natl Acad Sci U S A 98 (23), 12984-12989 (2001)
   PUBMED   11687650
REFERENCE   9  (bases 1 to 4853)
  AUTHORS   Crawford,B.E., Olson,S.K., Esko,J.D. and Pinhal,M.A.
  TITLE     Cloning, Golgi localization, and enzyme activity of the full-length
            heparin/heparan sulfate-glucuronic acid C5-epimerase
  JOURNAL   J Biol Chem 276 (24), 21538-21543 (2001)
   PUBMED   11279150
REFERENCE   10 (bases 1 to 4853)
  AUTHORS   Li,J.P., Gong,F., El Darwish,K., Jalkanen,M. and Lindahl,U.
  TITLE     Characterization of the D-glucuronyl C5-epimerase involved in the
            biosynthesis of heparin and heparan sulfate
  JOURNAL   J Biol Chem 276 (23), 20069-20077 (2001)
   PUBMED   11274177
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123610.10.
            
            On or before Feb 5, 2019 this sequence version replaced
            XM_006511571.2, NM_033320.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927938.2399834.1, AF325532.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AC123610.10        112053-112177       c
            126-216             AC123610.10        77997-78087         c
            217-815             AC123610.10        59583-60181         c
            816-1061            AC123610.10        57953-58198         c
            1062-4853           AC123610.10        46812-50603         c
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 33.56 cM"
     gene            1..4853
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /note="glucuronyl C5-epimerase"
                     /db_xref="GeneID:93683"
                     /db_xref="MGI:MGI:2136405"
     exon            1..125
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /inference="alignment:Splign:2.1.0"
     exon            126..216
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /inference="alignment:Splign:2.1.0"
     exon            217..815
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..2086
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /EC_number="5.1.3.17"
                     /note="heparin/heparan sulfate:glucuronic acid
                     C5-epimerase; heparan sulfate-glucuronic acid
                     C5-epimerase; heparan sulfate C5-epimerase; heparin
                     sulfate C5-epimerase; heparosan-N-sulfate-glucuronate
                     5-epimerase"
                     /codon_start=1
                     /product="D-glucuronyl C5-epimerase"
                     /protein_id="NP_201577.3"
                     /db_xref="CCDS:CCDS23262.1"
                     /db_xref="GeneID:93683"
                     /db_xref="MGI:MGI:2136405"
                     /translation="MRCLAARVNYKTLIIICALFTLVTVLLWNKCSSDKAIQFPRHLS
                     SGFRVDGLEKRSAASESNHYANHIAKQQSEEAFPQEQQKAPPVVGGFNSNGGSKVLGL
                     KYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSK
                     VYAQRSPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYG
                     LSHYSKNLTEKPPHIEVYETAEDRDRNIRPNEWTVPKGCFMASVADKSRSTNVKQFIA
                     PETSEGVSLQLGNTKDFIISFDLKLLTNGSVSVVLETTEKNQLFTVHYVSNTQLIAFR
                     DRDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFL
                     DNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQ
                     AISTLVRAYLLTKDYVFLSSALRATAPYKFPSEQHGVKAVFMNKHDWYEEYPTTPSSF
                     VLNGFMYSLIGLYDLKETAGETLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHF
                     MLGIAPNLARWDYHTTHINQLQLLSTIDESPIFKEFVKRWKSYLKGSRAKHN"
     exon            816..1061
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..4853
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4824..4829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /note="hexamer: AATAAA"
     polyA_site      4849
                     /gene="Glce"
                     /gene_synonym="1110017N23Rik; C130034A12Rik; Hsepi"
                     /note="major polyA site"
ORIGIN      
        1 acacgagcgc ttcctcgccg agaggctgga gctgcggcag cgcaggcctg ggccgccgtg
       61 tctctgccgc tcgcttcagt tttcctcagg gctctggggg ccgctcgtcc gccgacgcgc
      121 ctcaggaatt taacaagaaa ctgaagtttt gattcaaata ttttttgaat tgaagccaga
      181 aatgtactaa gattttgatt ctttcatttg attaaggtat ggtctgaata tgcgttgttt
      241 ggcagctcgg gtcaactata agactttgat tatcatctgt gcgctattca ctttggtcac
      301 agtacttttg tggaataagt gttccagcga caaagcaatc cagtttcctc ggcacttgag
      361 tagtggattc agagtggatg gattagaaaa aagatcagca gcatctgaaa gtaaccacta
      421 tgccaaccac atagccaaac agcagtcaga agaggcattt cctcaggaac aacagaaggc
      481 accccctgtt gttgggggct tcaatagcaa cgggggaagc aaggtgttag ggctcaaata
      541 tgaagagatt gactgtctca taaacgatga gcacaccatt aaagggagac gagaggggaa
      601 tgaagttttc cttccattca cttgggtaga gaaatacttt gatgtttatg gaaaagtggt
      661 ccagtatgac ggctatgatc gatttgaatt ctctcatagc tattccaaag tctatgcaca
      721 gagatcacct tatcaccctg acggtgtgtt tatgtccttt gaaggctaca atgtggaagt
      781 ccgagacaga gtcaaatgta taagtggagt tgaaggtgtg ccattatcta cccagtgggg
      841 gcctcaaggc tatttctacc caatccagat tgcacagtat gggctaagtc attacagcaa
      901 gaatctaacc gagaaacccc ctcacataga agtatatgaa acagcagaag acagggacag
      961 aaacatcaga cctaatgaat ggactgtgcc caaggggtgc ttcatggcca gtgtggcaga
     1021 caagtctaga tccaccaatg ttaaacagtt tattgctcca gaaaccagtg aaggtgtgtc
     1081 tttgcagctg ggaaacacaa aagacttcat tatttcattt gacctcaagc ttttaacaaa
     1141 tgggagtgtg tctgtggttc tggagaccac agaaaagaat cagctcttca ctgtgcatta
     1201 tgtctcaaac acccagctga ttgctttcag agacagggac atatactacg gcattgggcc
     1261 cagaacttca tggagtacag ttaccagaga cctggtcact gacctcagga aaggagtggg
     1321 cctttctaac acaaaagctg tcaagccaac caaaatcatg cccaaaaagg tggttaggtt
     1381 gattgcaaaa gggaagggat tcctggacaa cattaccatc tcaaccacag cccacatggc
     1441 tgcattcttt gctgcaagtg actggctagt gaggaaccag gatgagaaag gtggctggcc
     1501 aattatggtg acccggaagt taggggaagg gtttaaatct ttagaaccag gatggtactc
     1561 tgccatggca caagggcaag ccatctctac cttagtcagg gcctatcttc taacgaaaga
     1621 ctatgtattc ctcagttcag ctttaagggc aacagcccca tacaagtttc cgtcagagca
     1681 gcatggagtt aaagccgtgt tcatgaataa acatgactgg tatgaagaat atccaaccac
     1741 acctagctct tttgttttaa atggctttat gtattcttta attgggctgt atgacctaaa
     1801 agaaacagca ggggagacac ttgggaaaga agcaaggtcc ttgtacgagc gcggcatgga
     1861 atctcttaaa gccatgctgc ccttgtatga tactggctcc gggaccatct atgacctccg
     1921 ccacttcatg cttggcattg ctcccaacct ggcccgctgg gactatcaca ccacccacat
     1981 taaccagctg cagctgctca gcaccatcga tgagtcccca atcttcaaag aatttgtcaa
     2041 gaggtggaaa agctacctta aaggcagtag ggcaaagcac aactagagct cgaaaccaaa
     2101 agtgcattcc agcctcttct gtgtcgctca gcagcagagc tcagcagcac gtcgcacact
     2161 ttaaatgctg tgttctgggt ttgtgggtca cagcactgta agaagagacc cttaaagtta
     2221 gtcttctgaa acagttgcat tccacggggc aggtgcttag aaaggtatcg aggagagcgc
     2281 tctgtcagtg cactgaacca agacctctgc cctctcatca tgtaggcctc catgcagagt
     2341 cactctcagt ctgaggaaga tactggtaag tcactgctgc tagcctgctg cccagcactt
     2401 acctaaaact tagtatggag tgcttttaaa atgtgattat ttatatttat actgaaagca
     2461 ggcttttaga aatcagtgtg ctgtattaca gtaaatgtgt gcgtatagcc tggatcgtgg
     2521 acttttttaa gtgtttcttt cccataggtt catttcttga gagggagtga gcatttgttg
     2581 catttgttca attatatatg gaaaatattt tgaattatgg ttttcttgaa ctctgtatat
     2641 ctaaacacag cccgtgttca ggtttcacgg tcacatacat aagcacaact gaaatgaaac
     2701 ttgttgactg cacaagaagt cacagacatg ctgtgtttta tgaaacggtc tacaatcagt
     2761 caaagtttga taatcgtctt ccccacgtct gctctagtgc agtgtgtctg tatcactatc
     2821 tagaaggctg catttcattt catactatcc tttagtagtc ctgtccaatg agaggacatt
     2881 acacagaata ttaattataa gcctaaactt gtgacagact tgatgctttg aaattgacca
     2941 ttggtacctc ctccttaaaa gagaagtgtc gtgttcagga gtcacagtaa agtctctcct
     3001 gcctgggttg tcaccattgc ttttcacgtc gtttctgtcc ttgctgtttc tctgaagctt
     3061 aattttgaac gattccagca ttgaaacaga aaagatgcta ctgtgtctgc tgtctgtgtg
     3121 ccatcagatc tgtaagtgag tttcagagtc ctcatgccat ggacagtgtc tgctgtaagg
     3181 ctccgggcat taggtaaaag caacagggat tggtttgctc tggtttttgt ttagggtgtg
     3241 ataatataga gacagttggg taacataagc ctcacccttt ctaggaacaa ataatgacac
     3301 ttatccatgc tgctggtcag atgcatgtta aaggcgaggt gggtgatctg cccacacact
     3361 gtacttctgc tgaaaaatac acacacacac aataatacat atgtaaacta tatcagcatc
     3421 aaaggagtca ggaaagaaga gctgtactga agacaatatt aaactgttga aaaataaact
     3481 tagactcgca gtatatatga ccacacctta cttccaccta caaggtagct agtgtggtca
     3541 gtcaaacggc tccccagctt ttacctctgt ggttctcaca cacgaggtgt ttcagcaaca
     3601 tctgaacatg cacatttgtc tcagcaataa aagcgaagac tcccttttat tttcagttcc
     3661 tatgttgaaa acaagctggc ccacctggga acactgtcca cttctcagat ttgttactgg
     3721 agtggccccg acttctgctt tctgtttgca tactggtctt cagatgagag gagtcagtga
     3781 aatcatcgtg gaactgattt tgtgacaaac accagtgcct taccaatgtg gttgtccttt
     3841 tccactgaag agggaaagtt ggtttgtttg ggtttgtttt ggtttctgtt tctgtttttg
     3901 atgtagaaat ggcagttcac tgctgttgct tttccactgc cttccacgtt gcaacagcta
     3961 cttgacttat cttgtgatcc cagaatcccc taagaggtca cactgagcct gtgacagcac
     4021 tgtcagtgct tggcttgtca gacgtgaggg aggatgcctc tgtgtcactg ggggtaacaa
     4081 aatgagactc tgggtgtgca ggaattctgc ctgtgaagtg ggatccacag catgttcgac
     4141 gggtgggaga actgggcatg tatggaggaa cactgccagg gacccatgca tttttgcctc
     4201 cagaaaacta caaagttccc taggttttta aatcatgtaa atgtttactt atcctgagta
     4261 tagagagaat ggggagggta ttttaatttt ttgttgtgat tctttttttt ttaaggatat
     4321 tggtttggtt cacttctctg agttagcttc catgttgaat ctgtcaccat ttcagtagta
     4381 aaacagccaa aagtttctag aaaagagcag gtttgacctt taggagttac tgtatataac
     4441 ggcttcgttc aagagcctca cgacatctgt gaggtgtgta gaaatgtcac aagcactctc
     4501 cagatgcagg gcctctgtct ttgttggtgt acagactctt accaaaagct gctggtttgt
     4561 tcttgtaatt gtttccttgc ttctccattg tacatgtggg gtatatgcca tgtgtcaaat
     4621 atgcaataat gtgtttactg gatgccctcc tggagggtgg tcctctgtaa agctgtacag
     4681 cctttgcagt tcaagcacaa tgtgcacatg ttagttttat atacagcaaa acttgatttt
     4741 ttcaaatgga aaggtatttt gtttctatac aaagttaaat gggttgtttg tgcttaatgt
     4801 aaagctgctt tataaaattg taaaataaag tttttatctc gaaaagaaat gta
//
