LOCUS       NM_212444               4427 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus glycerol kinase (Gk), transcript variant 2, mRNA.
ACCESSION   NM_212444
VERSION     NM_212444.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Zapateria,B., Sevillano,J., Sanchez-Alonso,M.G., Limones,M.,
            Pizarro-Delgado,J., Zuccaro,A., Herradon,G., Medina-Gomez,G. and
            Ramos-Alvarez,M.P.
  TITLE     Deletion of pleiotrophin impairs glucose tolerance and liver
            metabolism in pregnant mice: Moonlighting role of glycerol kinase
  JOURNAL   FASEB J 35 (10), e21911 (2021)
   PUBMED   34551152
  REMARK    GeneRIF: Deletion of pleiotrophin impairs glucose tolerance and
            liver metabolism in pregnant mice: Moonlighting role of glycerol
            kinase.
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4427)
  AUTHORS   Miao,L., Su,F., Yang,Y., Liu,Y., Wang,L., Zhan,Y., Yin,R., Yu,M.,
            Li,C., Yang,X. and Ge,C.
  TITLE     Glycerol kinase enhances hepatic lipid metabolism by repressing
            nuclear receptor subfamily 4 group A1 in the nucleus
  JOURNAL   Biochem Cell Biol 98 (3), 370-377 (2020)
   PUBMED   31813231
  REMARK    GeneRIF: Glycerol kinase enhances hepatic lipid metabolism by
            repressing nuclear receptor subfamily 4 group A1 in the nucleus.
REFERENCE   4  (bases 1 to 4427)
  AUTHORS   Iwase,M., Tokiwa,S., Seno,S., Mukai,T., Yeh,Y.S., Takahashi,H.,
            Nomura,W., Jheng,H.F., Matsumura,S., Kusudo,T., Osato,N.,
            Matsuda,H., Inoue,K., Kawada,T. and Goto,T.
  TITLE     Glycerol kinase stimulates uncoupling protein 1 expression by
            regulating fatty acid metabolism in beige adipocytes
  JOURNAL   J Biol Chem 295 (20), 7033-7045 (2020)
   PUBMED   32273338
  REMARK    GeneRIF: Glycerol kinase stimulates uncoupling protein 1 expression
            by regulating fatty acid metabolism in beige adipocytes.
REFERENCE   5  (bases 1 to 4427)
  AUTHORS   So,V., Jalan,D., Lemaire,M., Topham,M.K., Hatch,G.M. and Epand,R.M.
  TITLE     Diacylglycerol kinase epsilon suppresses expression of p53 and
            glycerol kinase in mouse embryo fibroblasts
  JOURNAL   Biochim Biophys Acta 1861 (12 Pt A), 1993-1999 (2016)
   PUBMED   27713003
REFERENCE   6  (bases 1 to 4427)
  AUTHORS   Huq,A.H., Lovell,R.S., Ou,C.N., Beaudet,A.L. and Craigen,W.J.
  TITLE     X-linked glycerol kinase deficiency in the mouse leads to growth
            retardation, altered fat metabolism, autonomous glucocorticoid
            secretion and neonatal death
  JOURNAL   Hum Mol Genet 6 (11), 1803-1809 (1997)
   PUBMED   9302256
REFERENCE   7  (bases 1 to 4427)
  AUTHORS   Bassett,D.E. Jr., Boguski,M.S., Spencer,F., Reeves,R., Kim,S.,
            Weaver,T. and Hieter,P.
  TITLE     Genome cross-referencing and XREFdb: implications for the
            identification and analysis of genes mutated in human disease
  JOURNAL   Nat Genet 15 (4), 339-344 (1997)
   PUBMED   9090377
REFERENCE   8  (bases 1 to 4427)
  AUTHORS   Guo,W., Lovell,R.S., Zhang,Y.H., Huang,B.L., Burris,T.P.,
            Craigen,W.J. and McCabe,E.R.
  TITLE     Ahch, the mouse homologue of DAX1: cloning, characterization and
            synteny with GyK, the glycerol kinase locus
  JOURNAL   Gene 178 (1-2), 31-34 (1996)
   PUBMED   8921887
REFERENCE   9  (bases 1 to 4427)
  AUTHORS   Huq,A.H., Lovell,R.S., Sampson,M.J., Decker,W.K., Dinulos,M.B.,
            Disteche,C.M. and Craigen,W.J.
  TITLE     Isolation, mapping, and functional expression of the mouse X
            chromosome glycerol kinase gene
  JOURNAL   Genomics 36 (3), 530-534 (1996)
   PUBMED   8884278
REFERENCE   10 (bases 1 to 4427)
  AUTHORS   Makalowski,W., Zhang,J. and Boguski,M.S.
  TITLE     Comparative analysis of 1196 orthologous mouse and human
            full-length mRNA and protein sequences
  JOURNAL   Genome Res 6 (9), 846-857 (1996)
   PUBMED   8889551
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672056.4 and AL645567.7.
            
            On May 13, 2006 this sequence version replaced NM_212444.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the central coding region compared to variant 4. The
            encoded isoform (2) is shorter than isoform 4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088373.1, SRR13948564.4046636.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-394               AL672056.4         65735-66128         c
            395-468             AL672056.4         50643-50716         c
            469-575             AL672056.4         49884-49990         c
            576-653             AL672056.4         43641-43718         c
            654-730             AL672056.4         35534-35610         c
            731-868             AL672056.4         33023-33160         c
            869-978             AL672056.4         30376-30485         c
            979-1045            AL672056.4         29604-29670         c
            1046-1081           AL672056.4         26623-26658         c
            1082-1149           AL672056.4         26142-26209         c
            1150-1192           AL672056.4         20192-20234         c
            1193-1273           AL672056.4         19511-19591         c
            1274-1352           AL672056.4         4974-5052           c
            1353-1449           AL672056.4         3651-3747           c
            1450-1534           AL672056.4         2583-2667           c
            1535-1562           AL672056.4         2001-2028           c
            1563-1655           AL645567.7         111247-111339       c
            1656-1799           AL645567.7         110990-111133       c
            1800-1880           AL645567.7         109699-109779       c
            1881-1967           AL645567.7         104886-104972       c
            1968-4427           AL645567.7         100585-103044       c
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 39.32 cM"
     gene            1..4427
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="glycerol kinase"
                     /db_xref="GeneID:14933"
                     /db_xref="MGI:MGI:106594"
     exon            1..394
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     CDS             317..1978
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /EC_number="2.7.1.30"
                     /note="isoform 2 is encoded by transcript variant 2;
                     glycerokinase; ATP:glycerol 3-phosphotransferase"
                     /codon_start=1
                     /product="glycerol kinase isoform 2"
                     /protein_id="NP_997609.1"
                     /db_xref="CCDS:CCDS41050.1"
                     /db_xref="GeneID:14933"
                     /db_xref="MGI:MGI:106594"
                     /translation="MAAAKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIK
                     QEFPREGWVEQDPKEILQSVYECIEKTCEKLGQLNIDISNIKAIGVSNQRETTVVWDK
                     VTGEPLYNAVVWLDLRTQSTVENLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDN
                     VKKVQEAVEENRALFGTIDSWLIWSLTGGIHGGVHCTDVTNASRTMLFNIHSLEWDKE
                     LCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQDGQAK
                     NTYGTGCFLLCNTGHKCVFSEHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRD
                     NLGIIKSSEEIEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHI
                     AFAALEAVCFQTREILDAMNRDCGIPLSHLQVDGGMTSNKILMQLQADILYIPVVKPS
                     MPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQINAEESEIRYSTWKKAVM
                     KSIGWVTTQSPESGDPSIFCSLPLGFFIVSSMVMLIGARYISGIP"
     exon            395..468
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            469..575
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            576..653
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            654..730
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            731..868
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            869..978
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1045
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1081
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1149
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1192
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1273
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1352
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1449
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1534
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1655
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1799
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1880
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1967
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..4427
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3034..3039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="hexamer: AATAAA"
     polyA_site      3053
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="major polyA site"
     regulatory      3746..3751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="hexamer: AATAAA"
     polyA_site      3769
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="major polyA site"
     regulatory      4345..4350
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="hexamer: AATAAA"
     polyA_site      4364
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
     regulatory      4407..4412
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
                     /note="hexamer: ATTAAA"
     polyA_site      4427
                     /gene="Gk"
                     /gene_synonym="D930012N15Rik; Gyk"
ORIGIN      
        1 gtctcacaac cctcagactg gcctgctcct gggtctcttt ctgtagtcgc tcattggccc
       61 gcccggaaga cagcgccccc ccatttgcca cgcctcccaa ggcctaggag aatgcaggtc
      121 ggggcggggc ctgagctgga ggggcggggc gacgcggccg ggcgggagta aaggggctgc
      181 ctaggagccg cgcgagcctt cagcagaagc gcgctgcttt gccctccgct gtccctgctc
      241 cgccctccgc agccctccac agtcaccccg gagaccagcc gtgttaagct ctctgctctg
      301 aagctgactg acttccatgg cagccgcgaa gaaagcagtt ctggggccat tggtgggagc
      361 agtggaccag ggtaccagct cgacacgttt tttggttttc aattcaaaaa cagctgaact
      421 tcttagtcat catcaagtag aaataaaaca ggaattccca agagaaggat gggtagaaca
      481 agacccgaag gaaattctgc agtctgttta tgagtgtata gagaaaacgt gtgagaaact
      541 tggacagctc aatattgata tttccaacat caaagccatt ggtgtcagca accagaggga
      601 aaccacagta gtctgggaca aggtcaccgg agagcctctc tataatgccg tggtgtggct
      661 tgacctaaga acccagtcta ctgttgagaa ccttagtaaa agaattccag gaaataataa
      721 ctttgtcaag tccaagacag gccttccact tagcacgtat ttcagtgcag tgaaacttcg
      781 ttggctcctt gacaacgtga aaaaggtcca agaggctgtt gaagaaaata gagctctttt
      841 tgggaccatt gattcatggc ttatttggag tttaacagga ggaatccatg ggggtgtcca
      901 ctgtacagat gtaacaaatg caagcaggac gatgcttttt aacattcatt ctttggaatg
      961 ggataaagag ctctgcgaat tttttggaat tccaatggaa attcttccca acgttcggag
     1021 ttcttctgag atctatggcc taatgaaagc tggggccttg gaaggtgtac caatatctgg
     1081 gtgtttgggg gaccagtctg ctgctttggt gggacaaatg tgcttccagg atggacaggc
     1141 caaaaacacg tatggaacag ggtgcttctt attgtgcaac acgggccata agtgtgtatt
     1201 ttctgaacat ggcctcctga caaccgtagc atataaactt ggcagagaca aacctgtgta
     1261 ttatgcgttg gaaggttccg tggctatagc tggtgctgta atccgctggc taagagacaa
     1321 ccttggaatt attaagtcct ctgaggaaat tgaaaaactt gctaaggaag taggtacttc
     1381 ttatggctgc tacttcgttc cagcattttc agggttatat gcgccttatt gggagcccag
     1441 tgcaagaggg atcatctgtg gactcactca gttcaccaat aaatgtcata tcgcttttgc
     1501 tgcactagaa gctgtttgtt tccaaacccg agagattttg gatgccatga atcgcgactg
     1561 tggaattcca ctcagtcatt tacaggtaga tggaggaatg accagcaata aaattcttat
     1621 gcagctacaa gcagacattc tgtatattcc agtagtgaaa ccctccatgc ctgaaacaac
     1681 tgcactaggc gctgccatgg cagctggggc tgcagagggg gttggtgtgt ggagtcttga
     1741 acctgaggat ttgtcagctg tcacaatgga gcggtttgaa cctcagatca atgctgaaga
     1801 aagcgaaatc cgttactcca catggaagaa agctgtgatg aagtcaattg gttgggttac
     1861 aactcagtct ccagaaagtg gtgaccctag tatcttctgt agtctgcctt tgggcttttt
     1921 tatagtgagt agcatggtaa tgttaatcgg agcaaggtac atctcaggta tcccataaat
     1981 aataccacct cacggatttc caagatgcaa gctttttaat gtgatatgaa aatctgacta
     2041 ttctgtctca tagtataatg atgctattca tagactctga tttttttcat aagccactgg
     2101 ctgcatgatc ctctaagcag acctatgact tgaaataaag aaagtgcagc agaaagaatc
     2161 ctccagaaac atttaatttt tttttaacat tgacagttaa gatcgggtca gtcacctttg
     2221 aggctgacct ctgcctccac tgtcatgatg tcctacacta ttcccgttaa ggtctagggt
     2281 gattttgtat cctgtctatt gaaatgtgcc attcagtata ttcagatgct agtggattac
     2341 acatgtttga ggaagaggtt gttactaacc tgttcaaaat gagtggcttc ttgcttgttt
     2401 gcttttaaca gctcagatgt cttcttttct atatattaga aggccacaac attactggat
     2461 atttcaaatg gaaacatcta aagaattgtt ggataatgat ttgctattct tgtggcttag
     2521 acattttctg tacagtgttt gccaaaattc aaccttgtca ggtgttttac actgtccact
     2581 aactaccata gctttctgtc tggctcttac aggatagaac actttctttt tctgcttttt
     2641 tttcatttct cctttttata tttttattct gtatgtataa catacatgcc tatatatttt
     2701 atatgctgag agtaacccat ttataaatta agagcacatt atattcaata agttataaga
     2761 gggctggtct taagtggact actatgtata taaattgggg gagggcaagc tgtatacatt
     2821 tttgggcaac ggttatgcat attatttacc agaagaaaat tttcttataa aatcaaacat
     2881 ttgaaactta agtatgtatt cagtcaatta aaatgtacta tattgtaatt tcaactatat
     2941 ttcttatact ctaccattta attatctcag ctggaagaaa agcaactgtg gcatgtaaca
     3001 gtaaatactc ttctcaaaca tgattctagt ccaaataaaa tttttccttt gaattgatgt
     3061 tttccatatt tgagctccta ttgaagaatt atcaaattca aaagctaagg ggaaaaatat
     3121 gtgtgaataa agccaataat gccttttaaa tgttattacc cattaataag atgttcttat
     3181 tttctggtat gatagaaaat tatgtctact taataactga caaaaaaaaa acaagtatca
     3241 ttgcaaatgc ctcacagtat aaccagtaac ttctcaaata tttagaagga ttggttataa
     3301 ctcattaacc tagcttccta acatcaattt taaaatgtat ttgttttata gtaaaaaaaa
     3361 aagaaaagga tactttataa ttgttttgtt ttgtcatttt ggaatggaaa atattgatat
     3421 cttaatagaa aaagttttat ttggaattaa tggtagattg tccttcccct ctgcatgtgc
     3481 tgctcccaat gactgaagct gctcggtgtc actgatacag ctgccactta cgtgacatca
     3541 atatagaaag atcctgtgaa gttattatgg cagtgatact tttaaactta gttatatttc
     3601 tacaagggca ggttgagttc attgtaaata attgtgtaaa tcactgttct agtaatttaa
     3661 aaataacatt aatttttcta caaaatgcga gatttataaa tgtttgaaat tgtacctatt
     3721 gataattaat ggtaagttta cttaaaataa atgtatccct tatttcttat ctgcatttct
     3781 catttccaga tgaaagttta gttatcagag atgtttcagc acacacaaaa gccacttttc
     3841 agtacttgtt cacatgcact gacgagctaa ttagcagcag aagtggtttc tggaatgatg
     3901 aacaccttaa tcattctgtt agaacagatt agacagttcc tacctcagca ttttcagaaa
     3961 acacactttc aaggccaaaa gccatcatca tcagtagagt ctccccttgc tgagacctaa
     4021 ttatctccgg ggttccatcc tttacctcag cttctgtcta gctgggttag tcagtgaggc
     4081 ctggaacact gttgctcttt catactaatg ccaacatcac cacattttgt tctcccattg
     4141 atccgtaaaa taaataatgc attaatgctt cttcattggt acccccccct tttttttgag
     4201 acagagtctc actatgtagg cctgactgtt ttttgagaca gagtctcact atgtaggcct
     4261 gactgtccta caactcactc tgtataccaa gctggccttg aattcataga gatctgcctg
     4321 cctctgtctc ttccaactgc tgggaataaa gacatatgcc accatgcaca gaccatcaaa
     4381 tcttttttaa aattttcttg tggagtatta aaattaagta gctttta
//
