LOCUS       NM_153086               2613 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus gap junction protein, delta 4 (Gjd4), mRNA.
ACCESSION   NM_153086
VERSION     NM_153086.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wurmser,M., Chaverot,N., Madani,R., Sakai,H., Negroni,E.,
            Demignon,J., Saint-Pierre,B., Mouly,V., Amthor,H., Tapscott,S.,
            Birchmeier,C., Tajbakhsh,S., Le Grand,F., Sotiropoulos,A. and
            Maire,P.
  TITLE     SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell
            properties during fetal epaxial myogenesis
  JOURNAL   Development 147 (19) (2020)
   PUBMED   32591430
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2613)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2613)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2613)
  AUTHORS   von Maltzahn,J., Wulf,V., Matern,G. and Willecke,K.
  TITLE     Connexin39 deficient mice display accelerated myogenesis and
            regeneration of skeletal muscle
  JOURNAL   Exp Cell Res 317 (8), 1169-1178 (2011)
   PUBMED   21272575
  REMARK    GeneRIF: Data show that myogenesis in Cx39 KO mice is accelerated
            by increased myogenin expression on ED13.5 and 16.5 and increased
            Cx43 in developing skeletal muscle, and suggest that Cx43 may
            compensate for loss of Cx39 during myogenesis and regeneration.
REFERENCE   6  (bases 1 to 2613)
  AUTHORS   von Maltzahn,J., Wulf,V. and Willecke,K.
  TITLE     Spatiotemporal expression of connexin 39 and -43 during myoblast
            differentiation in cultured cells and in the mouse embryo
  JOURNAL   Cell Commun Adhes 13 (1-2), 55-60 (2006)
   PUBMED   16613780
  REMARK    GeneRIF: Upon differentiation into myotubes, Cx39 expression
            increased in mouse embryo.
REFERENCE   7  (bases 1 to 2613)
  AUTHORS   von Maltzahn,J., Euwens,C., Willecke,K. and Sohl,G.
  TITLE     The novel mouse connexin39 gene is expressed in developing striated
            muscle fibers
  JOURNAL   J Cell Sci 117 (Pt 22), 5381-5392 (2004)
   PUBMED   15466892
  REMARK    GeneRIF: connexin39 gene is expressed in developing striated muscle
            fibers
REFERENCE   8  (bases 1 to 2613)
  AUTHORS   Buniello,A., Montanaro,D., Volinia,S., Gasparini,P. and Marigo,V.
  TITLE     An expression atlas of connexin genes in the mouse
  JOURNAL   Genomics 83 (5), 812-820 (2004)
   PUBMED   15081111
REFERENCE   9  (bases 1 to 2613)
  AUTHORS   Sohl,G. and Willecke,K.
  TITLE     An update on connexin genes and their nomenclature in mouse and man
  JOURNAL   Cell Commun Adhes 10 (4-6), 173-180 (2003)
   PUBMED   14681012
  REMARK    Review article
REFERENCE   10 (bases 1 to 2613)
  AUTHORS   Willecke,K., Eiberger,J., Degen,J., Eckardt,D., Romualdi,A.,
            Guldenagel,M., Deutsch,U. and Sohl,G.
  TITLE     Structural and functional diversity of connexin genes in the mouse
            and human genome
  JOURNAL   Biol Chem 383 (5), 725-737 (2002)
   PUBMED   12108537
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC108777.7.
            
            On Sep 28, 2022 this sequence version replaced NM_153086.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053016.1, AK034663.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               AC108777.7         180698-180903       c
            207-2613            AC108777.7         176749-179155       c
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 4.91 cM"
     gene            1..2613
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /note="gap junction protein, delta 4"
                     /db_xref="GeneID:225152"
                     /db_xref="MGI:MGI:2444990"
     exon            1..206
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1237
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /note="connexin39; connexin-39; connexin u"
                     /codon_start=1
                     /product="gap junction delta-4 protein"
                     /protein_id="NP_694726.2"
                     /db_xref="CCDS:CCDS29049.1"
                     /db_xref="GeneID:225152"
                     /db_xref="MGI:MGI:2444990"
                     /translation="MEKLNLLGFLIITLNCNVTIMGMIWLIVEVLLRMLVVVLAGSPI
                     YEDEQERFICNTLQPGCANVCYDLFSPVSPLRFWLVQSLALLLPSVVFGTYTLHRGAK
                     LAAVGGACRPQVPDLSTAYLVHLLLRMLLEAGLAFLHYFLFGFSVPARVSCSHVPCSG
                     AVDCYVSRPTEKSLLILFFWAVSALSFLLSLADLLWILPRRKTLRTTQWVNGEARPVC
                     EVPAPPPCLLQNPQGYLSQGQVDQEDRQEEQVVPEFPCMWTAGQSDNSNVGQACVSGL
                     LEHSDQDASEATSSAGDRLTVAHTAHELRFHRETSLDLGGKNTQADELSLATQSHLAR
                     HSSASKPQAPCRLTTSGSAPHLRTKKSEWV"
     exon            207..2613
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2592..2597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /note="hexamer: AATAAA"
     polyA_site      2613
                     /gene="Gjd4"
                     /gene_synonym="9430022F06Rik; Cx39; Cxnu"
                     /note="major polyA site"
ORIGIN      
        1 agaagtcctg acctcccagg cctgggagca catctttctt tgcacggctt ggtgcttact
       61 tcaaagtctg atacagaaaa aattcattga tttttaaaca tccttggagt acacgagccc
      121 ggacctgtgg ttttcttcaa gcatggagaa gttgaacttg ttgggattcc tcatcatcac
      181 cttaaactgt aacgtgacca tcatgggcat gatctggctg atcgtggagg tcttgctgag
      241 gatgctagtg gtggtcttgg cagggtcacc tatctatgag gatgaacaag agaggtttat
      301 ttgcaacaca ctgcaaccag gatgtgccaa cgtttgctac gacctctttt ccccagtgtc
      361 accgctgcga ttctggctag tgcagagcct ggccttgctt ctgccttcgg tggtctttgg
      421 cacttacacc ctacaccgcg gtgcgaagct ggctgcagtg gggggagcct gcaggcccca
      481 ggtgcccgac ctgtctactg cctacctggt gcacctactg ctgcgcatgc tgctggaggc
      541 cgggctggcc ttcctgcact actttctctt tggcttttct gtgcccgccc gcgtgtcttg
      601 ctcgcatgta ccctgctcag gggctgtgga ctgctacgtg tcgcggccca cggagaagtc
      661 actcctgata ctattctttt gggcagtgag tgcgctatcc ttcctgctca gcttggctga
      721 cctgctttgg atcctgccga ggagaaagac actgaggacc acgcagtggg tgaatggaga
      781 ggctagacca gtctgtgaag tacctgcacc tcccccttgc ctcttacaaa acccccaggg
      841 ctatcttagc caaggtcagg tggaccaaga ggacagacag gaggaacaag ttgtgcctga
      901 gttcccctgc atgtggacag cagggcagag tgacaacagc aatgttggtc aggcctgtgt
      961 gtcggggctg ctggaacatt cagaccaaga tgctagtgag gccacttcct cagctggtga
     1021 caggctaaca gtggctcaca cagcacatga gctcagattc cacagagaga cttcactgga
     1081 cctggggggc aaaaacaccc aggcagatga actctccttg gctacccaga gccacctggc
     1141 cagacacagt tcagccagca agcctcaagc tccatgccgg ctgaccacct caggcagtgc
     1201 tccccatttg agaaccaaaa aatctgagtg ggtgtgaaag cagccacgca gagccccaaa
     1261 accctttttt ttctagcacc gtactgccct ttgtactcaa ggcagggtaa gaggtttctt
     1321 catcttgtat tcttgctgag atcattttat atttaactag aaattttatt tccatggtat
     1381 agaatcccat gaaaaactat cctacactgt tctaaagaac ctgaggtcaa gacggtccag
     1441 ctatcttact ttctgttaaa ctgcttgctt gggcaaacct cctcctccat ctaatcactt
     1501 ttattgaact gtttgattaa gaaaaacctc cctctgcagc tttggagcaa gtcaccaagg
     1561 tgccctgcag ggcctaggat ggccttggta ggggtggaat gacatggttc ctacccctga
     1621 aacaggggca acagggcatg ggcctacatg agtgtcaatg gctgctgtaa ggtttgtttg
     1681 tataatactg gacatatttt tacacacctc ttttaaggat tgtcttccct gttaacgtta
     1741 atgactccat gctaatcagg gatagcatga gtgggggagg caagggtgtt gctgatgtct
     1801 ctgcaaactt gtaaatgttg ggatggtcct tcaggatagt ccttaggctg tgagaagaac
     1861 ctctagaagc atgagttaaa atagacataa tttctcaact atgcaaaata taaggatgca
     1921 atatgaatta tatgggggct tcacaaactg aaaggaacaa aagcagctat actgtaaacc
     1981 aacttgtcag aaagctacta aggagggaga tggagagatt gtggagtggt gatctcagag
     2041 atcttgccta gctaagattc ttgtttgtgc ttacaaaggc agagcaaggt taggcccaga
     2101 catgatcaca cctagttaac agacatagaa agatctatga actcttttgc aaggtttaaa
     2161 aatatatgtg tttgctgtct cctaagaacc aagaggctgg ggtcatggga tgctgattca
     2221 taggataatc aaaagggaac ctggagtaaa tggctggatt gatatgtaaa ataaaagact
     2281 gggcttagga tctgcaatat tttagaatag caagacagaa ctgcttggag aactgtctag
     2341 agtagaaaac agaaagagct gtctggagat ctccagagaa agcagatcag agagaaaatc
     2401 ttgcaagcag aacttaagtt gtcagcttgg acccatgatt tgacttcgat ttgtttgttt
     2461 caatgctctc aaatacccct tctctctctc caagtgaagg ctggttcttg gcaaaaaaaa
     2521 aaaatcctta ccctaaaatc tcagagctac acttgggtcc ccaatacctg aatgtagttc
     2581 cagtcagctg taataaagac tttcaattgc cta
//
