LOCUS       NM_008125               2404 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus gap junction protein, beta 2 (Gjb2), mRNA.
ACCESSION   NM_008125
VERSION     NM_008125.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Huey,E.L., Turecek,J., Delisle,M.M., Mazor,O., Romero,G.E., Dua,M.,
            Sarafis,Z.K., Hobble,A., Booth,K.T., Goodrich,L.V., Corey,D.P. and
            Ginty,D.D.
  TITLE     The auditory midbrain mediates tactile vibration sensing
  JOURNAL   Cell 188 (1), 104-120 (2025)
   PUBMED   39701100
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Qin,T., So,K.K.H., Hui,C.C. and Sham,M.H.
  TITLE     Ptch1 is essential for cochlear marginal cell differentiation and
            stria vascularis formation
  JOURNAL   Cell Rep 43 (4), 114083 (2024)
   PUBMED   38602877
REFERENCE   3  (bases 1 to 2404)
  AUTHORS   Xu,K., Chen,S., Bai,X., Xie,L., Qiu,Y., Liu,X.Z., Wang,X.H.,
            Kong,W.J. and Sun,Y.
  TITLE     Degradation of cochlear Connexin26 accelerate the development of
            age-related hearing loss
  JOURNAL   Aging Cell 22 (11), e13973 (2023)
   PUBMED   37681746
  REMARK    GeneRIF: Degradation of cochlear Connexin26 accelerate the
            development of age-related hearing loss.
REFERENCE   4  (bases 1 to 2404)
  AUTHORS   Li,R., Wang,T., Marquardt,R.M., Lydon,J.P., Wu,S.P. and DeMayo,F.J.
  TITLE     TRIM28 modulates nuclear receptor signaling to regulate uterine
            function
  JOURNAL   Nat Commun 14 (1), 4605 (2023)
   PUBMED   37528140
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2404)
  AUTHORS   Kersbergen,C.J., Babola,T.A., Kanold,P.O. and Bergles,D.E.
  TITLE     Preservation of developmental spontaneous activity enables early
            auditory system maturation in deaf mice
  JOURNAL   PLoS Biol 21 (6), e3002160 (2023)
   PUBMED   37368868
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2404)
  AUTHORS   Leff,S.E., Brannan,C.I., Reed,M.L., Ozcelik,T., Francke,U.,
            Copeland,N.G. and Jenkins,N.A.
  TITLE     Maternal imprinting of the mouse Snrpn gene and conserved linkage
            homology with the human Prader-Willi syndrome region
  JOURNAL   Nat Genet 2 (4), 259-264 (1992)
   PUBMED   1303276
REFERENCE   7  (bases 1 to 2404)
  AUTHORS   Miragall,F., Hwang,T.K., Traub,O., Hertzberg,E.L. and Dermietzel,R.
  TITLE     Expression of connexins in the developing olfactory system of the
            mouse
  JOURNAL   J Comp Neurol 325 (3), 359-378 (1992)
   PUBMED   1332989
REFERENCE   8  (bases 1 to 2404)
  AUTHORS   Schwarz,H.J., Chang,Y.S., Hennemann,H., Dahl,E., Lalley,P.A. and
            Willecke,K.
  TITLE     Chromosomal assignments of mouse connexin genes, coding for gap
            junctional proteins, by somatic cell hybridization
  JOURNAL   Somat Cell Mol Genet 18 (4), 351-359 (1992)
   PUBMED   1332196
REFERENCE   9  (bases 1 to 2404)
  AUTHORS   Stutenkemper,R., Geisse,S., Schwarz,H.J., Look,J., Traub,O.,
            Nicholson,B.J. and Willecke,K.
  TITLE     The hepatocyte-specific phenotype of murine liver cells correlates
            with high expression of connexin32 and connexin26 but very low
            expression of connexin43
  JOURNAL   Exp Cell Res 201 (1), 43-54 (1992)
   PUBMED   1319348
REFERENCE   10 (bases 1 to 2404)
  AUTHORS   Hennemann,H., Kozjek,G., Dahl,E., Nicholson,B. and Willecke,K.
  TITLE     Molecular cloning of mouse connexins26 and -32: similar genomic
            organization but distinct promoter sequences of two gap junction
            genes
  JOURNAL   Eur J Cell Biol 58 (1), 81-89 (1992)
   PUBMED   1322820
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK146485.1 and BB756861.1.
            
            On May 24, 2009 this sequence version replaced NM_008125.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146485.1, BC013634.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2392              AK146485.1         2-2393
            2393-2404           BB756861.1         508-519
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 30.1 cM"
     gene            1..2404
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /note="gap junction protein, beta 2"
                     /db_xref="GeneID:14619"
                     /db_xref="MGI:MGI:95720"
     exon            1..234
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /inference="alignment:Splign:2.1.0"
     exon            235..2404
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..937
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /note="connexin-26; gap junction membrane channel protein
                     beta 2; connexin e"
                     /codon_start=1
                     /product="gap junction beta-2 protein"
                     /protein_id="NP_032151.1"
                     /db_xref="CCDS:CCDS27153.1"
                     /db_xref="GeneID:14619"
                     /db_xref="MGI:MGI:95720"
                     /translation="MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVW
                     GDEQADFVCNTLQPGCKNVCYDHHFPISHIRLWALQLIMVSTPALLVAMHVAYRRHEK
                     KRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSIFFRVIFEAVFMYVFYIMYNG
                     FFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITELCYLFVRYC
                     SGKSKRPV"
     regulatory      2269..2274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /note="hexamer: AATAAA"
     polyA_site      2291
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
     regulatory      2370..2375
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /note="hexamer: AATAAG"
     polyA_site      2395
                     /gene="Gjb2"
                     /gene_synonym="Cnx26; Cx26; Cxne; Gjb-2"
                     /note="major polyA site"
ORIGIN      
        1 aggatcgcag cgcgcggcgc gctccgctct cccaactcgc agccagtcgg cggcgctcca
       61 gtccactgag cgcagcctcc accgggaccc gcggcgacca gcacaggatc agccggcgac
      121 ccatttcgga ccaacccagg agcggccccg ggacccgctt cagacctgct ccttaccggg
      181 gacagcgcct cgccgcttcc cgccacccaa cgctgcacgc tcctccggac acagtgccaa
      241 ccatccagag gacaagatgg attggggcac actccagagc atcctcgggg gtgtcaacaa
      301 acactccacc agcattggaa agatctggct cacggtcctc ttcatcttcc gcatcatgat
      361 cctcgtggtg gctgcaaagg aggtgtgggg agatgagcaa gccgattttg tctgcaacac
      421 gctccagcct ggctgcaaga atgtatgcta cgaccaccac ttccccatct ctcacatccg
      481 gctctgggct ctgcagctga tcatggtgtc cacgccagcc ctcctggtag ctatgcatgt
      541 ggcctaccgg agacatgaaa agaaacggaa gttcatgaag ggagagataa agaacgagtt
      601 taaggacatc gaagagatca aaacccagaa ggtccgtatc gaagggtccc tgtggtggac
      661 ctacaccacc agcatcttct tccgggtcat ctttgaagcc gtcttcatgt acgtctttta
      721 catcatgtac aatggcttct tcatgcaacg tctggtgaaa tgcaacgctt ggccctgccc
      781 caatacagtg gactgcttca tttccaggcc cacagaaaag actgtcttca ccgtgtttat
      841 gatttctgtg tctggaattt gcattctgct aaatatcaca gagctgtgct atttgttcgt
      901 taggtattgc tcaggaaagt ccaaaagacc agtctaatgc attgcctggc tgctagagca
      961 aagatggagg gagaggatga ggcaacccat gcttagtcgc ttagtcggca gagctcagcc
     1021 accagcagtt cccaagacaa acattcccat ctaaaatgcc accatttgaa gtccctggag
     1081 gcctcctatg aaactccaga agcctccgtg ggcctccctt cccccaaagc tcccaaacaa
     1141 aggcccaatt ctatgcctgt attaatgggt tctaaagtta gttccactga gaccccgtgc
     1201 tggtagcgac caagcttgga ggatacattc acagtttaaa caaagggatc tcacattgtt
     1261 tctcttcctc tgaggacagg agagaggaac ccggtcctga ggaaggtgcc actgagaaag
     1321 tccccccccc caagttaccc ctagctaaag gtaaagaaat gccatactgt tatttgcttt
     1381 gatagtttac gtgtgcaaca atggacaaaa agctattaat acttcaagtg ctgtgtactt
     1441 ttttttttta atgaaaacct taaaataaga taggctcttt tttttcctaa aatgatctgg
     1501 aaagtattat acattcctcc tagtccagag gttcagtgtg tgaagtgagc atggtgtgta
     1561 accagatctc acagggtctt taagatgttg gcctttgggt tatgggaaac ctgcagtgtg
     1621 gctgagggcc cacctattgt attcatcact ggcgtgctgg gctcagggct ataaccctac
     1681 tgcctgtcat aagtgagaca aactggaagc gtctcgtgct ggcttctgac aagaagaggc
     1741 ctcaattaaa atagtcaact gtaattttgt gtaagagaca gataggatat gccgacaact
     1801 gccacctgcc atgagcctaa ctcagcccca ccccaccccc accccccacc agtttgtcta
     1861 ctctgtagct gtgggatgtt gcagtcagca tcagaagata gggacttcat gggtttgctt
     1921 gggaatttca ctgccatggg acaattcaat ggtgcagaaa ccaaatgggg tggttttcag
     1981 agaaccaatg aaacttctag actcccaatc ctgttgatta aaactaagtt tttctacttt
     2041 tgaaagtgtg tttgcctccc tcttcagcat tgccttctta actggaaaca gaaatgtgga
     2101 tatttggaag aaatagaaga aactagttta ggtcagtatg taacctttct agggcaagtt
     2161 gaaccattac cactatgcct tatgtgtatc ctacaagatg acagtcaaca aatccaacag
     2221 gggacacttc ttcctgccaa gaatgtcatt ggaaagccat tttgtaacaa taaataagag
     2281 ttgtggtttc atgcttgtca ctgatataat tttgtaaacc cctaaatgcc ctttattaaa
     2341 atatgattat tttgtatact ttaaaattaa ataaggtttt taatagaata ccaaactcta
     2401 acgt
//
