LOCUS       NM_008123               6989 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus gap junction protein, alpha 8 (Gja8), mRNA.
ACCESSION   NM_008123
VERSION     NM_008123.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Sun,Q., Li,J., Ma,J., Zheng,Y., Ju,R., Li,X., Ren,X., Huang,L.,
            Chen,R., Tan,X. and Luo,L.
  TITLE     JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens
            Fiber Maturation in Murine Lens Development
  JOURNAL   Invest Ophthalmol Vis Sci 64 (15), 15 (2023)
   PUBMED   38095908
REFERENCE   3  (bases 1 to 6989)
  AUTHORS   Zhou,Y., Bennett,T.M., White,T.W. and Shiels,A.
  TITLE     Charged multivesicular body protein 4b forms complexes with gap
            junction proteins during lens fiber cell differentiation
  JOURNAL   FASEB J 37 (4), e22801 (2023)
   PUBMED   36880430
REFERENCE   4  (bases 1 to 6989)
  AUTHORS   Du,Y., Tong,Y., Quan,Y., Wang,G., Cheng,H., Gu,S. and Jiang,J.X.
  TITLE     Protein kinase A activation alleviates cataract formation via
            increased gap junction intercellular communication
  JOURNAL   iScience 26 (3), 106114 (2023)
   PUBMED   36852280
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6989)
  AUTHORS   Jara,O., Minogue,P.J., Berthoud,V.M. and Beyer,E.C.
  TITLE     Levels and Modifications of Both Lens Fiber Cell Connexins Are
            Affected in Connexin Mutant Mice
  JOURNAL   Cells 11 (18), 2786 (2022)
   PUBMED   36139360
  REMARK    GeneRIF: Levels and Modifications of Both Lens Fiber Cell Connexins
            Are Affected in Connexin Mutant Mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6989)
  AUTHORS   Schwarz,H.J., Chang,Y.S., Lalley,P.A. and Willecke,K.
  TITLE     Chromosomal assignments of mouse genes for connexin 50 and connexin
            33 by somatic cell hybridization
  JOURNAL   Somat Cell Mol Genet 20 (3), 243-247 (1994)
   PUBMED   7940024
REFERENCE   7  (bases 1 to 6989)
  AUTHORS   Evans,C.W., Eastwood,S., Rains,J., Gruijters,W.T., Bullivant,S. and
            Kistler,J.
  TITLE     Gap junction formation during development of the mouse lens
  JOURNAL   Eur J Cell Biol 60 (2), 243-249 (1993)
   PUBMED   8330621
REFERENCE   8  (bases 1 to 6989)
  AUTHORS   Runge,P.E., Hawes,N.L., Heckenlively,J.R., Langley,S.H. and
            Roderick,T.H.
  TITLE     Autosomal dominant mouse cataract (Lop-10). Consistent differences
            of expression in heterozygotes
  JOURNAL   Invest Ophthalmol Vis Sci 33 (11), 3202-3208 (1992)
   PUBMED   1399425
  REMARK    Review article
REFERENCE   9  (bases 1 to 6989)
  AUTHORS   White,T.W., Bruzzone,R., Goodenough,D.A. and Paul,D.L.
  TITLE     Mouse Cx50, a functional member of the connexin family of gap
            junction proteins, is the lens fiber protein MP70
  JOURNAL   Mol Biol Cell 3 (7), 711-720 (1992)
   PUBMED   1325220
REFERENCE   10 (bases 1 to 6989)
  AUTHORS   Favor,J.
  TITLE     A comparison of the dominant cataract and recessive specific-locus
            mutation rates induced by treatment of male mice with
            ethylnitrosourea
  JOURNAL   Mutat Res 110 (2), 367-382 (1983)
   PUBMED   6877261
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CV557810.1, GU363513.1, AC127297.4 and CF732960.1.
            
            On Nov 1, 2012 this sequence version replaced NM_008123.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GU363514.1, GU363513.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142, SAMN02146468
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               CV557810.1         2-122
            122-1674            GU363513.1         1-1553
            1675-6608           AC127297.4         32218-37151
            6609-6989           CF732960.1         1-381
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3"
     gene            1..6989
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="gap junction protein, alpha 8"
                     /db_xref="GeneID:14616"
                     /db_xref="MGI:MGI:99953"
     exon            1..186
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /inference="alignment:Splign:2.1.0"
     exon            187..6971
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..1515
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="connexin-50; lens fiber protein MP70; lens opacity
                     10; connexin l; gap junction membrane channel protein
                     alpha 8; alpha 8 connexin"
                     /codon_start=1
                     /product="gap junction alpha-8 protein"
                     /protein_id="NP_032149.1"
                     /db_xref="CCDS:CCDS17651.1"
                     /db_xref="GeneID:14616"
                     /db_xref="MGI:MGI:99953"
                     /translation="MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFV
                     WGDEQSDFVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLMYVGHAVHHVRM
                     EEKRKDREAEELCQQSRSNGGERVPIAPDQASIRKSSSSSKGTKKFRLEGTLLRTYVC
                     HIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKTIFILFMLS
                     VAFVSLFLNIMEMSHLGMKGIRSAFKRPVEQPLGEIAEKSLHSIAVSSIQKAKGYQLL
                     EEEKIVSHYFPLTEVGMVETSPLSAKPFSQFEEKIGTGPLADMSRSYQETLPSYAQVG
                     VQEVEREEPPIEEAVEPEVGEKKQEAEKVAPEGQETVAVPDRERVETPGVGKEDEKEE
                     LQAEKVTKQGLSAEKAPSLCPELTTDDNRPLSRLSKASSRARSDDLTI"
     regulatory      1550..1555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="hexamer: AATACA"
     regulatory      1562..1567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="hexamer: GATAAA"
     polyA_site      1571
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="major polyA site"
     polyA_site      1584
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="major polyA site"
     regulatory      6949..6954
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
                     /note="hexamer: AATAAA"
     polyA_site      6971
                     /gene="Gja8"
                     /gene_synonym="Aey5; Cnx50; Cx50; Cxnl; dcm; Lop10"
ORIGIN      
        1 gtaaaaccca cccatcagga cagatccagt catatctgct cagtcgtgca gcacattagg
       61 ggcggggtag ggaagttgga ggtgggcttt taggtggcaa aggaaagaca ccaaagttgc
      121 cattttactg cggagcagca agagagaaag acagcaccag tttctccgtg ggacgctact
      181 cagacgtgag caatgggcga ctggagtttc ctgggaaaca tcttggaaga ggtgaatgag
      241 cactccactg tcatcggcag agtctggctc acagtgctct tcatcttccg catcctcatc
      301 ctcgggacag cagcggagtt tgtgtggggc gatgagcaat ctgattttgt atgcaacacc
      361 cagcagccag gctgtgagaa tgtctgctac gatgaggcct ttcccatctc acacatccgc
      421 ctctgggtgc tgcagatcat cttcgtctcc actccatcgc tgatgtacgt ggggcacgcg
      481 gtacaccacg ttcgcatgga ggagaagcga aaggaccgtg aagctgagga gctctgtcag
      541 cagtcgcgca gcaacggggg tgagagggta ccaatcgccc cagaccaggc cagcatccgg
      601 aagagcagca gcagtagcaa aggcaccaag aagttccggc tggagggcac actgctaagg
      661 acctatgtct gccacatcat cttcaagacc ctctttgagg tgggcttcat cgtgggccat
      721 tacttcctgt atggtttccg catcctgccc ctctatcgct gcagccggtg gccctgcccc
      781 aatgtggtag actgctttgt atcccggcct actgagaaga ccatcttcat cctcttcatg
      841 ttatcagtcg cttttgtgtc actcttcctc aacatcatgg agatgagcca cctgggcatg
      901 aaaggaatcc ggtctgcctt caagaggcct gtagagcaac cactggggga gattgctgag
      961 aagtccctcc actccattgc agtttcctcc atccagaaag ccaagggcta ccagcttcta
     1021 gaagaagaga agatcgtatc acactatttc cctttgacag aggttggaat ggtggagacc
     1081 agccctcttt cggccaagcc ttttagtcag tttgaggaga agatcggcac aggacccctg
     1141 gcagatatgt cacggagtta ccaagaaacc ctgccttctt atgctcaggt gggggtccag
     1201 gaagtggagc gggaagagcc gcctatagaa gaggctgtgg aaccggaagt gggagagaag
     1261 aagcaagaag cagagaaggt ggccccagaa gggcaggaga cagttgcagt gccagacagg
     1321 gagagagtag agacccctgg agtggggaag gaggatgaga aagaagagct gcaagctgaa
     1381 aaggtaacca agcaagggct gtctgctgag aaggcaccct cactctgtcc ggagctgaca
     1441 accgatgaca atcggccctt gagcaggctg agtaaagcca gcagcagggc caggtcagat
     1501 gatctcacca tatgacctgg tgcagaagaa agatatccca cctccagcca atacagacca
     1561 agataaaccc agctggtgcc aatatacttt gagtcttatg tgtcccttgg tccaccccca
     1621 cccctggttt gatagatgct gttctagaac aatgcacgct gtacaactag gggactgcct
     1681 ctcccacctg tatgcgctgt caaggtaaac ccgctgtcct agtgacgttc ctatgcctgc
     1741 cctggtcttc tatcccaact gctgttagga aggtggggat gggggctttg ctttcctcct
     1801 ccattctcca tggaaaccca tcaggtcaga atcatgatta cctcataacc ttttcctaat
     1861 cttctctcgt gctcactcac tcagtcagtg agattctgta ccccagcacg tccccagcag
     1921 acgccaccta ccctacatga gagatctcaa actcaaaagc ctataggacc caggccagtg
     1981 atttaaatag caaatgtttc catgctgtaa tatgacagag agaggtaggg tcatgaaaga
     2041 tggagagcgg gataacagcc cgccccttcc aggcagtggt ttccttgatg cctgggcatc
     2101 tggtttgttt gtttgtttgt ttgtttgttt ttcttggttg ttgttttatg agaaacaaaa
     2161 atataattgg aattttaaca agttaatttt aaatacaata tgcggaccaa atgcaatgta
     2221 tccaaattta acctgtgtgg tagtaagttg caatatcagt tttttacatt aatgagccca
     2281 gatttgctaa gaaattggtc tcactttttt tttaatggtc ttggcctatt ttttgtggtt
     2341 tcccaaccgc atcttatcac tttgaaatgt cttaagtcat cagtccagag atgcccagag
     2401 ttcttgctca cctttctgtt gggtcactgt gccccagggt ttaaagcaat cgagcctttg
     2461 tatcagaaag aattcagccc agagcacagt gagagaacaa gtgagctatc agacctctcc
     2521 ctgtgaaatg aaaagttttt aggctttcaa gccaattgca gttttcttcc ctgaagcaac
     2581 aaaaatcctt ttttaaaatt ctcttcttcc aacatcagtc aacctaaaat ctgcatctcc
     2641 tttcaccaag gctgggagct ggacaagttc caaactggaa gatatctgcc cataggcctc
     2701 tcctgaggga ggcagctctc aagcattgct cagacagaat gagcaccaaa aagctgccag
     2761 cagaaatgtg acagggggga aacatgtctt actctattac ctacaaactg gacgctcagt
     2821 cagtacccag tccaagctgt atccctaaaa gagatgcaga gactagaggg gcctctcata
     2881 ggcctcatcc cctataagcc ttctacaaac ctcactgagc ccatcagggg aaaaaaatat
     2941 caagtatacg tatatatttc acacagggaa caatttcact caatcaatcg atttttcaga
     3001 acagatgata tatagataga taaatgatag ttggatagat agatcagtag atagagcaat
     3061 gatagataga tagatagata gatagataga tagatagata gatagataga tatagataga
     3121 tatagataga cagatagaca aacatgttag atgatatgaa taaaatcagg cgatggtgac
     3181 cttgatatct aactatctga ttttaaaaaa aaagaaggca gaaattcatg gcaagaggct
     3241 tcctgatcag cccaggatag ccatgcatgc agcccaacac aaaatcataa aagtacagtc
     3301 ttagtttgtg taactattgc tatgatgaaa caccatgatc aaaaagcaag ctagggagga
     3361 aagggtttat ttggcttatg tatccacact gtcatctatc actgaaggaa acccagacag
     3421 caatttaaac agggcaggaa ccaggaggca ggagctgatg cagaagccac agagggggaa
     3481 ctgcttattg tcatgcttgc tcctcatggc ttcgcacagc ttgcttgctt atagacccca
     3541 ggcctaccac ctacaatggg ctgggccttc catatcaatc actaattaag aaaatgtcct
     3601 acaggttcac ctacagccag atcttatgga ggcattttct caattgagga tccctcctct
     3661 caaatgactg cagcttatat caagttgaca taaaactctc cagcacactt acttaaaata
     3721 ttatgagatt tcttttgtaa ctttttttgt gcttaaaatg catgagtctc tagtgtgaac
     3781 actgtagagg atgggatcat ggtgtcaaga gtctgaacac acctgaaccc tatgcctcat
     3841 ggaccacatg ccaaggatgg gtagggagta gagaaactaa gttaacaggg cagagacttg
     3901 aacaggatac cagggtaccc agcaaaactg ccctttgatt ggcctcacta cctgccagca
     3961 tcacccaagg tgagaaagtc cttttcaacc ttctggctac catcttcatt ttaacatatt
     4021 tgctctggat aagccttcag aaactatccc tccctctgaa agttctcatt gcctggtgcc
     4081 acattctcag ccataacagt ttcaaagata aactcactga atggtttagt tttatttttt
     4141 tataagtatg cctaggaaat ctatagttaa aagaaaagga ctaactatat atgtatctag
     4201 aaaaccaaag taacaaactt tcagtggagg aatgtttcag gcaatggaca gtgagtagaa
     4261 ggagacatcc acctgcccgg cacgggactt tctgcttact gctggaatct gaccccacca
     4321 gtcaatagca tccaggcaca gaccctgccg tgtaccatga gaacggtaac caaatcccaa
     4381 gcctgagacc ccagagtgtt cttgaccctc acaggctgcc ctctcaccct atagttatgc
     4441 ttcccattta aaaagaacct tctttctcaa ggctgagaga gttcaagctg ggcacctcaa
     4501 ctaagaccaa attttaaaaa gaacaaaatc ctgtttcaag gtgatgttgc cagtggtgtt
     4561 tgaaactagt tgcagagctg tgagggaggg gtgctggcct ccttgccttg gctggacgct
     4621 agcaagttac tccctcgcat gaaagaagca gtgtctagct tccctgccca cacctgcttg
     4681 gagcctcaca tatcctgcca tttccttttg cccttcccaa caaaaaggca tataggttgg
     4741 aagaagcaga cacaggcacc aacaacaggg actttcttcc tacttctgcc agttagtggc
     4801 tatgtcatca ctgcctctta ccctctctat acctccattt cctgtctaca aacaggtaat
     4861 ggcactactg ccttgctacc tccagggtta tcgaaaccca tgagttggct accacacttg
     4921 gagcctgctt tgtgacgcca cttcctgaga gcttcctcca acagcagtag gacaggatag
     4981 agaacaggtg ggtggctgcc atctttctca gaaaaaactc agcaaaatct tggttccata
     5041 taatgccggg gataggtagg cggttactta agtgattgtt acacgacatc tcttcttcag
     5101 ctgccgctgg gctcctgtca ggtcccttca gaggtacctg tgtgtctgtg ctctttgtag
     5161 ctcatccctg ccctctgttt gggggacaaa taatcatgac tgacccagga gtatgatgct
     5221 ctggattcca ttgggtccag ctttacttcc aactcagtcc ctcattagat aaatccctag
     5281 agcacatcgt cctaccaatt aaagatgcct tggctatcaa gagaacagga gtgaggctcc
     5341 tcccagagga aaagaaggag ccattggctt gatagcggta gtaactccac catcgggaga
     5401 ggtctgtacc aggaagccta gaatcaggtt cagtcatgtt gtttgggtgc tccctagcac
     5461 tggggctttg taaaaagacc tatctggttg tcatgatttt agcccttgct ggtcagatga
     5521 tgcatccaac acagcattga acaaatgttc ctgaagatca ggatggctta gagactcaca
     5581 agactcactg acatttgtaa ggtgtggcag ctgtctcttt ctttccaact ctggagatgc
     5641 caagttatca tttctcagct ctctatgaca gttttctcta ggttgatagg gtatatgagc
     5701 ccccctcctt tacaaagtct aatttgtctt ctcaaaaact tactaatgtg aaaagaaaaa
     5761 gagaagaaag aaggcgctgt ccaaactgta gggatcacat tgtaaagtac tccttaagga
     5821 ccaatgcttg tccctcctga acaaagcaca gcttgttaac cggagagaca gatgaactgt
     5881 gggacagcaa gtgaaatgag ggatgatcca gaaggcctaa gaatcggacc acacacaaga
     5941 acagctttca tcccatgaga atctgttgca agtgatgcta aggggttcat cttaccagag
     6001 ctcatgtaca gacaaggaag gatatgtcaa cctctgagac tgacttgagc acacaggaac
     6061 tgctcagctg aagccccagg gaaggctatg ggcaagactg gtgcagacca ccattcttca
     6121 gttcctgagg ggacgaaggg cacacaaagg gtgagaatga gccaggaacc caacagaagc
     6181 tcttatgcca ctccatcctc ttccagtctt cttcctctga ctccagacca gccctttatg
     6241 tctatcattc acaaacaatt gcccagccaa agccggtaca gcctaggtaa aaagtgtggg
     6301 gagacaggtt gaatggaaaa ctcctctaag cataaatatg ttctgtaaga tgtagtgtgt
     6361 acctagaaat tatgagagac gagacaggga ggttccccta acttgaaatt attgtagtat
     6421 aaataaatat gtctttgggg aaagggagga gataaaggaa acataattct agattcagct
     6481 attaaataag aagataactc agtctccact gcttttggca ctgctgctcc cagagacagg
     6541 agaagatgcc attcaactct ggcagtggcc acacaggtgg tgggggcagg gtaggggcag
     6601 gaggaggggg acagactaga gtctctactg ccttctggaa gtttcgaggg cagaacacaa
     6661 attctagaag aattcagaaa tttgcccgaa gataaaagga acttgctcct ttgccttctg
     6721 tgtgtgcccc ctgccatctg agaaacataa gtaaagaagg agctaagaga tgcactcaaa
     6781 gttagccgca ctgggaggag ctaagagatg cactcaaagt tagcatcact gggaaaccct
     6841 catgagcagc cacactggga ggatgggtgg gatttttcaa tctgtatttt ccaaccctcc
     6901 ccactcctgt gtcagaacta ataactacgt tagattgtta acaaaacaaa taaaactaac
     6961 tcgactttta aaaaaaaaaa aaaaaaaaa
//
